Biosan IW-8 Bedienungsanleitung

Contents

1. About this edition of user instructions.......................................................................3

2. Safety precautions ...................................................................................................4

3. General information..................................................................................................6

4. Getting started..........................................................................................................7

5. Operation ...............................................................................................................11

6. Operation closedown .............................................................................................15

7. Program error messages........................................................................................16

8. Specifications.........................................................................................................16

9. Ordering information ..............................................................................................17

10. Maintenance and troubleshooting..........................................................................18

11. Warranty.................................................................................................................21

12. Glossary.................................................................................................................22

13. EU Declaration of conformity..................................................................................23

1. About this edition of user instructions

The current edition of the user instructions applies to the following models and

versions of plate washers:

IW-8, Intelispeed Washer...................................................................version V.4AW

2. Safety precautions

Caution! Make sure you have fully read and understood the present Manual

before using the equipment. Please pay special attention to sections

marked by this symbol.

2.1. GENERAL SAFETY PRECAUTIONS

The protection provided can be ineffective if the operation of the appliance does not

comply with the manufacturer's requirements.

Save the unit from shocks and falling.

After transportation or storage and before connecting it to the electric circuit, keep the

unit under room temperature for 2-3 hrs.

Store and transport the unit at ambient temperatures between -20°C and +60°C and

maximum relative humidity of 80%.

Before diagnostics, study carefully the possibilities and operation procedures of the

unit in order to obtain reproducible and credible results. Check any unclear issues

with the supplier.

Do not leave the operating unit unattended for an extended period.

Change the bottles or connecting tubes only when the unit is switched off.

In case of doubt about the quality and uniformity of the completed washing program,

disregard the results obtained and repeat the washing procedure.

Accuracy of the obtained testing results depends on the quality of the microplate

rinsing procedure. To assure correctness of the rinsing procedure, regularly exercise

visual control over reproducibility of liquid volume dosing.

Before using any cleaning or decontamination methods except those recommended

by the manufacturer, check with the manufacturer that the proposed method will not

damage the equipment.

Do not make modifications to the design of the unit.

2.2. ELECTRICAL SAFETY

Connect only to an external power supply unit with voltage corresponding to that on

the serial number label. Use only the external power supply unit provided with this

product.

Do not plug the power supply unit into an ungrounded power socket, and do not use

an ungrounded extension lead.

Ensure that the switch and external power supply connector are easily accessible

during use.

Disconnect the unit from the mains before moving.

If liquid penetrates into the unit, disconnect it from the mains and do not use it until it

is checked by a repair and maintenance technician.

Do not operate the unit in premises where condensation can form. Operating

conditions of the unit are defined in section 8. Specifications.

2.3. DURING OPERATION

Do not operate the unit in environments with aggressive or explosive chemical

mixtures. Please contact manufacturer for possible operation of the unit in specific

atmospheres.

Do not operate the unit if it is faulty.

Always have the cover protecting against aerosol spread (hereinafter protective

cover) installed during operation.

Do not operate the unit if any of the needles is clogged or malfunctions.

Never touch the needles or place fingers or other parts under the needles during

operation. Manifold needles are sharp and can cause injury.

Always switch off the unit before changing the manifold.

If liquid spills on the guiding rail, stop the operation, clean and wipe the surface dry.

Do not allow the waste bottle to overflow the maximum level during operation (there

is a mark on the side surface of the bottle). Take necessary precautions utilizing

waste liquid in accordance with general laboratory standards.

2.4. AFTER OPERATION

Сarry out the cycle of system washing with distilled water and do not dry. This will

keep the system in permanent operation condition. Follow instructions outlined in

section 6. Operation closedown of this manual.

Clean the guiding rail under the platform and wipe it dry to prevent oxidation layer

formation and to prolong working life of the unit.

2.5. BIOLOGICAL SAFETY

It is the user’s responsibility to carry out appropriate decontamination if hazardous

material is spilt on or penetrates into the equipment.

In diagnostic researches, potentially dangerous biological materials can be used.

When working with such materials, always use protectiveclothing and eye protection.

Always have the protective cover installed during operation.

3. General information

Intelispeed Washer IW-8 is designed for washing of standard flat-bottom 96-well

plates during IFA, etc., in diagnostical laboratories. IFA is also performed in other areas,

including scientific research. The unit is fully programmable ensuring multi-step solution

ripening, aspiration (aspiration, combination of aspiration/liquid dispensing and soaking, as

well as soaking cycle during a particular period of time).

Standard version is supplied with 8-channel manifold for dispensing/aspiration, 3

channels with 3 bottles for washing and rinsing solutions and 2 bottles for waste and aerosol

collecting.

The unit has 101 user-defined editable programs (from 00 to 100).

Optional 4-channel washing solution weight logger 4CHW Logger is available. After

connecting it to IntelispeedWasher IW-8, the unit provides automatic control of reagent total

volume in all bottles that is necessary for washing and waste collecting and estimates the

volume of reagents necessary for washing.

Microbiologic suction filter eliminates risks of contamination caused by bacteria,

viruses, and infected particles from patient. Such contamination can be distributed by suction

pump or central vacuum units. Microbiological suction filter is hydrophobic and has very high

bacterial efficiency –up to 99.99% of particles bigger than 0.027 micrometres (which are

smaller than Hepatitis A, B and C).

The unit includes:

washing mode;

rinsing mode;

double aspiration;

additional solution mixing (shaking) mode during time gap between two work cycles;

soaking mode;

possibility to use plates of different manufacturers. This is ensured by automated

plate setup (adjusting to different depths of plate wells);

round-bottom plate and strip washing mode;

user-defined programs with adjustable parameters;

automatic storing of work programs.

4. Getting started

4.1. Unpacking. Remove packing materials carefully and retain them for future shipment

or storage of the unit. Examine the unit carefully for any damage incurred during

transit. The warranty does not cover in-transit damage.Warranty covers only theunits

transported in the original package.

4.2. Complete set. Numbers in the first column correspond to numbers on Figure 1.

Name

Quantity

IW-8 Intelispeed washer

1 piece

Platform for plates

1 piece

Manifold

1 piece

Protecting cover

1 piece

External power supply

1 piece

Tubes (outside/inside diam./length 6/3/600 mm)

5 pieces

Tube for manifold (outside/inside diam./length 3.2/1.6/400 mm)

1 piece

Tube for manifold (outside/inside diam./length 5/3/440 mm)

1 piece

Tube for hydrophobic filter (outside/inside diam. 9/6 mm)

1 piece

Half-litre bottle with connectors for aerosol collection

1 piece

1-litre bottles with sieve filters and connectors for reagents

3 pieces

2-litre bottle with connector for collecting of waste liquid

1 piece

Hydrophobic filters for half-litre bottle

2 pieces

Manifold cleaning set

1 piece

Syringe for liquid flushing in hoses

1 piece

Power cable

1 piece

User instructions, declaration of confirmity

1 copy

4-channel washing solution weight logger, 4CHW Logger

on request

Figure 1. Complete set

4.3. Storage and transportation.

- During long-term storage, keep the unit and all its accessories in original packaging

in a dry dust-free place.

- If the unit will be subjected to low temperatures, dry the tubes as described in 6.

Operation closedown.

- Use the original packaging for transportation whether transported by air, water or

land.

Attention! We do not accept warranty claims related to damages caused by

improper packaging.

- To pack the unit properly, see 10.8.

Figure 2. Unit side and back overview. Setting up

4.4. Setup.

4.4.1. Place the unit on a strong horizontal surface, which can safely support weight of the

unit. To provide optimal ventilation, ensure 100 mm clearance on each side.

Operation conditions are described in the Specification section.

4.4.2. Remove the protecting cover.

4.4.3. Unpack the plate platform.

4.4.4. Place the plate platform on the railing (fig. 2/1) so that the plate holder (flat spring)

faces the rear side of the unit (fig. 2/2). Magnet on the other side of the rail locks the

platform into start position.

Note: If the plate platform is installed improperly, the magnet does not hold

it in place and the platform will be able to move freely. Move the

platform along the railing until the magnets lock. Increase of

resistance to movement indicates that the magnets are locked.

4.4.5. Unpack the manifold.

4.4.6. Install the manifold in the slots of the holder arm (fig. 2/3). Magnets in the manifold

and the holder arm connect and align the manifold.

4.4.7. Connect the manifold inlet connector supplying the liquid and the blue-coloured

Washing Head Dispense connector at the rear panel of the unit (fig. 3/2) with a tube

(fig. 1/7).

4.4.8. Connect the manifold outlet connector and the yellow-coloured Washing Head

Aspirate connector at the rear panel of the unit (fig. 3/4) with a tube (fig. 1/8).

Note: Use the provided labels for marking bottles with washing solutions

and bottles for collecting waste liquid and aerosol.

4.4.9. Connect the #4 green-coloured Waste bottle outlet on the rear side of the unit to the

connector of 2-litre bottle #4 for waste liquid collecting (fig. 3/1).

4.4.10.Place the tube for the hydrophobic filter (fig. 1/9) on the connector of the half-litre

bottle #5, then place the hydrophobic filter on the tube (fig. 1/13). Check that the “IN”

marking on the filter is facing the bottle #5 (fig. 11).

4.4.11.Connect the Waste bottle #4 to the half-litre bottle #5 (fig. 3/10).

Figure 3. Assembly diagram

4.4.12.On the rear side of the unit:

4.4.13.Connect the bottle #1 to the inlet connector (#1 –red, fig. 3/3) with a tube (fig. 1/6).

4.4.14.Connect the bottle #2 to the inlet connector (#2 –black, fig. 3/5) with a tube (fig. 1/6).

4.4.15.Connect the bottle #3 to the inlet connector (#3 –white, fig. 3/6) with a tube (fig. 1/6).

4.4.16.Insert the manifold tube (fig. 3/2) into the valve opening (fig. 2/4). Press the valve in

the direction of the arrow and hold the valve while stretching the tube and pulling it

through the opening. Release the valve.

Note: Make sure that all connections are secured tightly.

4.4.17.Install the protective cover. Ensure that all tubes are covered, but are not squeezed.

4.4.18.Place bottles near the unit.

4.4.19.If 4-channel weight logger is included in the kit, remove pads from underside of scale

cups. Place bottles on the 4-channel weight logger according to the numbering.

Connect the RS-232 with the cable to the plug connector on the rear side of the unit

(fig. 3/7).

4.5. Switching on.

Warning! Before connecting the unit to the mains outlet, check that the power

switch (fig. 3/9) is in position O(off).

4.5.1. Connect the external power supply to the power socket (fig. 3/8) on the rear panel of

the unit.

4.5.2. Connect the power cord to the external power supply (fig. 1/16)

4.5.3. Connect the external power supply to the grounded power socket.

4.5.4. Switch on the unit (position I) using the power switch on the rear panel.

4.5.5. When switched on, the unit performs full initialisation cycle and displays the message”

Power on reset”

Note: Full initialisation cycle takes no longer than 5 seconds.

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