Fluigent ESS Bedienungsanleitung

USER’S

MANUAL

EASY

SWITCH

SOLUTIONS

MICROFLUIDIC VALVES

WARNINGS

Do not place the product in an unstable location with a level surface

and a strong and stable support

Do not use other power supply than the one provided with the ESSTM

The power supply provided with the ESSTM has been carefully selected

to meet the power requirements of the ESSTM in all conﬁgurations and

to comply with all safety standards.

If you are using the ESSTM platform with other ﬂow control systems,

please check that the pressure in your ﬂuidic system does not exceed

100 psi.

Aria is a perfusion system which automates perfusion or timed

injection protocols. It allows for the sequential delivery of up to 10

different solutions at the desired ﬂow rate into a microﬂuidic chip,

perfusion chamber or petri dish.

Do not open SWITCHBOARD, 2-SWITCHTM, M-SWITCHTM or L-SWITCHTM

devices. Please refer all servicing to after-sales service department

(support@ﬂuigent.com)

Prevent any objects or liquid from entering the SWITCHBOARD,

this may cause a short-circuit failure or other malfunction. Failing to

respect this advice would:

• Expose you to direct current/voltage in case the device is under

voltage which may lead to severe damages

• Void device's warranty

• Discharge our company from any liability regarding physical or

device damages.

INTRODUCTION

Fluidic circuits examples

2SWITCHTM VALVE

Fluidic principle

Product overview

Fluidic connections

Positionning

MSWITCHTM VALVE

Fluidic principle

Product overview

Fluidic connections

Positionning

LSWITCHTM VALVE

Fluidic principle

Product overview

Fluidic connection

Positionning

SWITCHBOARD

Product overview

Connection

HOW TO USE ESSTM WITH GAS

HOW TO START WORKING WITH THE ESSTM

FREQUENTLY ASKED QUESTIONS

TECHNICAL SPECIFICATIONS

SERVICING & WARRANTY

SUMMARY

The Easy Switch SolutionTM platform (ESSTM) provides solutions for path

selection in microﬂuidics. The Easy Switch SolutionsTM platform enables

one to implement 3 different kind of valves in a microﬂuidic circuit.

INTRODUCTION

1. The 2-SWITCHTM is a 3-port/2-position

solenoid valve: 2 ports can alternatively be

connected to a third one.

2. The M-SWITCHTM is a 11-port/10-position

rotary valve: 10 peripherical ports connected

to a central one.

3. The L-SWITCHTM is a 6-port/2-position valve

to inject precise volume of ﬂuid or for switching

ﬂuid to different channels.

4. The SWITCHBOARD is a hub that hosts up to 4 M-SWITCHTM or 4

L-SWITCHTM and up to 8 2-SWITCHTM. The device allows communication

between the PC with dedicated software : ESSTM Control for live control

or Microﬂuidic Automation Tool (MAT) to automate protocols, plus it

ensures power supply to the connected valves.

Combining the 2-SWITCH™, M-SWITCH™ and L-SWITCH™ valves

using the SWITCHBOARD allow one to access new possibilities to easily

design and build even the most complex microﬂuidic circuits. It is also

a powerful tool to simplify chip design and automate experiments.

INTRODUCTION

The ESS™ platform has been designed to work at its best

performances with FLUIGENT pressure-based ﬂow control solutions

(LineUpTM series MFCS™ series and Flow-Rate Platform).

It is however possible to use the ESS™ platform with other ﬂow

control systems provided that the pressure applied to the ESS™

devices does not exceed 7 bar (~ 100 psi)

Note: To ensure product longevity, the M-SWITCHTM should not be

used with an operational pressure above 5 bar (~72,5 psi)

The ESS™ user manual explains how to use the ESS™ series for

ones daily work. It will describe all the ESS™ functionalities that will

help rationalize microﬂuidic circuits and automate any experiments.

Examples of applications using the ESS™ are also described as well

as answers to the frequently asked questions about the ESS™. With

these elements one will be able to exploit fully the performance of the

Easy Switch Solutions™ platform for dedicated application.

INTRODUCTION

F  

In this application example, up to 10 liquids (4 on the schematic) are

selected sequentially inside the 1-channel chip by the M-SWITCHTM.

The samples at the outlet of the chip are sorted by a 2-SWITCHTM either

into a collector tube or into a waste. Each step can be automated

either by using Microﬂuidic Automation Tool (MAT) or partially with

live control and monitoring using ESSTM Control dedicated software.

S 

This type of set-up suits for applications such as:

• Cell analysis

• Cell lysis and DNA extraction (for PCR or NGS analysis)

• Drug testing

• Calibration curve

M-SWITCHTM

LineUpTM Flow EZ

FLOW UNIT

2-SWITCHTM

Manifold

Reagents

Chip

Recover Waste

Pressure

& power

supply

P-CAP

2-SWITCHTM

INTRODUCTION

In this application example, different concentration of the molecule

of interest are injected into the chip thus generating droplets with

different concentrations. These droplets are then sorted at the outlet

of the chip by the M-SWITCHTM depending on their concentrations.

Each step can be automated either by using Microﬂuidic Automation

Tool (MAT) or partially with live control and monitoring using ESSTM

Control dedicated software.

S   

M-SWITCHTM

Molecule

of interest

Water

Oil

Concentration

LineUpTM Flow EZ

This type of set-up suits for applications such as:

• Droplet generation

• Concentration dosage

• Sample preparation

INTRODUCTION

Several samples are injected simultaneously or separatly within a

Y-shaped chip by changing the position of the three 2-SWITCHTM

placed at the chip inlets. The injected solutions into the chip are

mixed by diffusion and the samples at the outlet are then sorted by

the fourth 2-SWITCHTM. Each step can be automated either by using

Microﬂuidic Automation Tool (MAT) or partially with live control and

monitoring using ESSTM Control dedicated software.

S 

This type of set-up suits for applications such as:

• Chemical mixing reactions

• Stoichiometry study

LineUpTM Flow EZ

FLOW UNIT

2-SWITCHTM

Reagents

Diffusive

mixing chip

Recover Waste

Pressure

& power

supply

P-CAP

2-SWITCHTM

INTRODUCTION

The L-SWITCH™ can be used as a useful cell culture tool: a small

volume of buffer can be recirculated within a closed loop into

the chip for several hours or days. Combined with the LineUpTM

pressure regulation it can achieve a highly stable ﬂow with a

positive impact on the shear stress. The Microﬂuidic Automation

Tool (MAT) allows to simultaneously and automatically switch

the valve position and the pressurized reservoir so that the ﬂow is

maintained unidirectionally without being stopped.

F 

P 1 P 2

This type of set-up suits for applications such as:

• Cell culture

• Shear-stress control

L-SWITCHTM

Buffer Buffer

LineUpTM Flow EZLineUpTM Flow EZ

MATMAT

INTRODUCTION

Sample from the reservoir is loaded into a "sample loop" between

two outlets of the L-SWITCHTM while carrier buffer is directly injected

into the chip. When the L-SWITCHTM position is switched the

controlled sample loop volume is injected into the chip along with

carrier buffer. The sample is then sorted by the 2-SWITCHTM at the

outlet of the chip.

C  

This type of set-up suits for applications such as:

• Cell culture

• PCR

LineUpTM Flow EZ

FLOW UNIT

RecoverWaste

P-CAP 2-SWITCHTM

Pressure

& power

supply

Waste

Carrier

Sample

L-SWITCHTM

S 

Detector

P 1 P 2

Chip

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