Millipore Pellicon Single Pass TFF Bedienungsanleitung
The life science business of Merck
KGaA, Darmstadt, Germany
operates as MilliporeSigma in the
U.S. and Canada.
Installation and User
Guide
Pellicon®Single-Pass TFF Accessories
®
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Pellicon®Single Pass TFF Accessories Installation and User Guide 3
Contents
Introduction...........................................................................5
Catalog Numbers ............................................................5
Pellicon®Single-Pass TFF Installation.........................................6
Pellicon®2 Mini Cassette Installation..................................6
Pellicon®2 Cassette Installation ........................................9
Pellicon®3 88 cm2or 0.11 m2Cassette Installation ............ 12
Pellicon®3 0.57 m2or 1.14 m2Cassette Installation........... 15
Flushing and Cleaning ........................................................... 18
Flushing....................................................................... 18
Cleaning ...................................................................... 19
Sanitization ......................................................................... 20
Depyrogenation.................................................................... 21
Integrity Testing................................................................... 22
Measurement of Normalized Water Permeability (NWP).............. 24
Storage............................................................................... 27
Storing the Filters in the System ..................................... 27
Storing the Filters without the System ............................. 27
Standard Product Warranty.................................................... 28
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Pellicon®Single Pass TFF Accessories Installation and User Guide 5
Introduction
This guide provides installation and maintenance procedures
for the Pellicon®Cassette Single-Pass TFF Accessories. It is not
intended to provide validation protocols or supporting data for
validation purposes. Refer to the appropriate Pellicon®Cassette
Validation Guide for this information. Refer to the Certificate of
Quality supplied with each Pellicon®cassette or hardware for
specifications. Refer to the appropriate cassette user guide for
additional information on installation and use.
The Pellicon®Cassette Single-Pass TFF Accessories must be
installed in the appropriate Pellicon®Cassette holder.
For supporting information on cassette installations, refer to the
following:
• Pellicon®2 Cassettes Maintenance, Installation and User Guide
• Pellicon®3 Cassettes Installation and User Guide
• Torque and Compression Force of Pellicon®2 and 3 Cassettes
Catalog Numbers
This user guide supports the following catalog numbers.
Catalog
Number Description Cassette Size
XXSPTFF01
Pellicon®Single-Pass TFF
Mini Kit
Includes four diverter
plates and two silicone
gaskets
Pellicon® 3 88 cm2
and 0.11 m2
Pellicon®2 0.1 m2
XXSPTFF02 Pellicon®Single-Pass TFF
cassette diverter plate
Pellicon® 3 0.57 m2and
1.14 m2
Pellicon®2 0.5 m2
XXSPTFF03
Pellicon®Single-Pass
TFF cassette retentate
collection plate
Pellicon® 3 0.57 m2and
1.14 m2
Pellicon®2 0.5 m2
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Pellicon®Single-Pass TFF
Installation
Pellicon®2 Mini Cassette Installation
Required Equipment
Items listed in this table are required for a three-section single-
pass TFF installation with Pellicon®2 Mini Cassettes. Sections may
be added or removed as needed for each specific application.
Item Description Quantity
Pellicon®Single-Pass TFF Mini Kit
(includes four diverter plates and two silicone gaskets) 1
Pellicon®2 Mini Cassette (includes two silicone gaskets) 3
Pellicon®Mini holder with extended tie rods 1
NOTES
Refer to the Pellicon®2 Cassette Installation Guide for cassette
installation instructions.
Install only identical cassettes in the filter holder. Do not mix
cassettes with different screens, membrane types, filtration
areas, or nominal molecular weight cutoffs.
Installation of Pellicon®2 Mini Cassettes requires inter-
cassette gaskets. Each cassette is provided with two gaskets.
The Pellicon®Single-Pass TFF Mini Kit provides two additional
gaskets, all of which are required for installation.
The diverter plates must be installed between individual
cassettes with the port locations alternating position as
shown in the following illustration.
Pellicon®Single Pass TFF Accessories Installation and User Guide 7
Installation
9
1
1
2
2
2
2
2
2
2
2
3
3
3
3
4
4
4
5
6
6
6
7
7
7
8
Components
Key Description
1 Holder end plate
2 Gasket
3 Pellicon®Single-Pass TFF Mini diverter plate
4 Pellicon®2 Mini Cassette
5 Tie rod spacer
Port Descriptions
Key Description User Supplied Connections
6 Feed/retentate ports Cap or pressure gauge with cap
7 Permeate ports Hose barb for tubing connection
and optional valve
8 Retentate port
Pressure gauge with hose barb
and pinch clamp, for retentate
outlet tubing
9 Blind permeate port Cap
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1. Loosen the nuts on the tie rods of the holder and remove the
nuts, washers, tie rod spacers, and end plate.
2. Slide the Pellicon®2 Mini Cassette gasket onto the holder so
that the tie rods pass through the cutouts in the gasket.
3. Slide the Pellicon®Single-Pass TFF mini diverter plate onto
the holder so that the tie rods pass through the cutouts in the
diverter plate. Note the location of the fittings on the diverter
plate.
4. Slide a gasket onto the holder.
5. Slide a Pellicon®2 Mini Cassette onto the holder.
6. Slide a gasket onto the holder.
7. Slide a diverter plate onto the holder so that the fittings on
the diverter plate are in the opposite location of the previous
diverter plate.
8. Slide a gasket onto the holder.
9. Install the next Pellicon®2 Mini Cassette.
10. Slide a gasket onto the holder.
11. Complete the installation with the required number of diverter
plates, alternating the location of the ports on the diverter
plates.
12. Slide a gasket onto the holder.
13. Install the end plate of the holder and torque the holder
using the specifications described in the Pellicon®2 Cassette
Installation Guide.
14. To nalize the serial ow path, cap or connect tubing to
the diverter plates ports (female luer lock) according to the
descriptions listed in the table in this section.
NOTE
For details on installation and torque specification, please
refer to the Pellicon®2 Cassette Installation Guide.
Pellicon®Single Pass TFF Accessories Installation and User Guide 9
Pellicon®2 Cassette Installation
Required Equipment
Items listed in this table are required for a three-section single-
pass TFF installation with Pellicon®2 Cassettes. Sections may be
added or removed as needed for each specific application.
Item Description Quantity
Pellicon®Single-Pass TFF Cassette diverter plate 3
Pellicon®Single-Pass TFF Cassette retentate
collection plate 1
Pellicon®2 Cassette (includes two silicone gaskets) 3
Pellicon®2 gasket (spare) 1
Pellicon®stainless steel holder 1
NOTES
Refer to the Pellicon®2 Cassette Installation Guide for cassette
installation instructions.
Install only identical cassettes in the filter holder. Do not mix
cassettes with different screens, membrane types, filtration
areas, or nominal molecular weight cutoffs.
Installation of Pellicon®2 Cassettes requires inter-cassette
gaskets. Each cassette is provided with two gaskets.
Installation with diverter plates requires one additional
gasket, which must be ordered separately.
The Pellicon®Single-Pass TFF Cassette retentate collection
plate is required to collect the retentate from the single-
pass TFF cassette installation.
The diverter plates must be installed between individual
cassettes with the tab locations alternating as shown in the
following illustration.
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Installation
1
2
3
5
1
2
2
2
2
2
3
2
3
4
4
4
Key Description
1 Holder end plate
2 Gasket
3 Pellicon®Single-Pass TFF Cassette diverter plate
4 Pellicon®2 Cassette
5 Pellicon®Single-Pass TFF Cassette retentate collection plate
1. Loosen the nuts on the tie rods of the holder and remove the
nuts, washers, tie rod spacers and end plate.
2. Slide the Pellicon®2 Cassette gasket onto the holder.
3. Slide the Pellicon®Single-Pass TFF cassette diverter plate onto
the holder so the tab on diverter plate is on the feed flow side
of the holder.
4. Slide a gasket onto the holder.
5. Slide a Pellicon®2 Cassette onto the holder.
6. Slide a gasket onto the holder.
7. Slide a diverter plate onto the holder so the tab on diverter
plate is on the opposite side of the previous diverter plate.
Dieses Handbuch passt für folgende Modelle
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