SCIEX ExionLC 100 Installationshandbuch
ExionLC™100 System
Hardware User Guide
May 2018RUO-IDV-05-1839-C
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1 Operational Precautions and Limitations...............................................................................................6
Electrical Precautions.........................................................................................................................................................6
AC Mains Supply..........................................................................................................................................................6
Protective Earth Conductor..........................................................................................................................................7
Environmental Precautions................................................................................................................................................7
Electromagnetic Environment......................................................................................................................................8
Decommissioning and Disposal....................................................................................................................................8
Ventilation Precautions......................................................................................................................................................9
Chemical Precautions.........................................................................................................................................................9
System Safe Fluids.....................................................................................................................................................10
Static Electricity Precautions ...........................................................................................................................................11
Typical Cause of Static Electricity Accidents...............................................................................................................11
Preventing Static Electricity Accidents.......................................................................................................................14
Equipment Use and Modification.....................................................................................................................................16
Maintenance, Inspections, and Adjustment.....................................................................................................................17
Storage and Handling......................................................................................................................................................18
2 Overview ...............................................................................................................................................19
Front Side.........................................................................................................................................................................20
Side View.........................................................................................................................................................................22
Rear Panels......................................................................................................................................................................24
Interior View....................................................................................................................................................................25
Flow Line Diagram...........................................................................................................................................................27
Entire Flow Line.........................................................................................................................................................27
Inner Volume of Each Flow Line.................................................................................................................................28
Flow Line During Sample Injection Operations................................................................................................................29
Ready.........................................................................................................................................................................29
Release of Pressure from the Flow Line.....................................................................................................................31
Needle Movement.....................................................................................................................................................32
Needle Rinse Before Sample Aspiration.....................................................................................................................33
Sample Aspiration......................................................................................................................................................35
Needle Rinse After Sample Aspiration.......................................................................................................................36
Start Analysis.............................................................................................................................................................37
Initialize the Measuring Pump...................................................................................................................................39
Rinse Solution Aspiration...........................................................................................................................................40
Rinse Solution Discharge............................................................................................................................................41
3 Configuration ........................................................................................................................................43
Configure Initial Settings.................................................................................................................................................43
Show the Calibration Tab...........................................................................................................................................43
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Contents
Set a Safety Lock........................................................................................................................................................52
Set Password Request to Deactivate the Lock............................................................................................................54
Configure Network Settings.......................................................................................................................................57
Set the Date and Time...............................................................................................................................................61
Change the System Pass ID........................................................................................................................................64
Change the Login Pass ID..........................................................................................................................................66
Initialize Settings........................................................................................................................................................69
Configure the System to Shut Down after Analysis....................................................................................................71
4 Operating Instructions...........................................................................................................................75
Sample Workflow.............................................................................................................................................................75
Turn on the HPLC System.................................................................................................................................................76
Log on to the HPLC System..............................................................................................................................................77
Prepare the Mobile Phase and Rinse Solution..................................................................................................................77
Select a Rinse Solution...............................................................................................................................................79
Install the Mobile Phase and Rinse Solution on the Reservoir Tray..................................................................................84
Perform an Autopurge.....................................................................................................................................................85
Install the Column............................................................................................................................................................90
Install the Column in the Column Oven.....................................................................................................................90
Equilibrate the Column..............................................................................................................................................95
Prepare the Sample..........................................................................................................................................................96
Put the Sample In a Sample Vial................................................................................................................................96
Put the Sample in the Autosampler...........................................................................................................................99
Removal of Sample Racks During Analysis....................................................................................................................101
Post-Analysis Procedures...............................................................................................................................................102
Rinse the Flow Line..................................................................................................................................................102
Turn Off the System.................................................................................................................................................102
5 Service and Maintenance....................................................................................................................104
Replace the Mobile Phase..............................................................................................................................................105
Replace the Mobile Phase with a Miscible Solvent..................................................................................................107
Replace the Rinse Liquid (if applicable)..........................................................................................................................111
Purge the Pump Manually..............................................................................................................................................115
Prime the Autosampler Rinse Solution Manually...........................................................................................................119
Change Sample Racks....................................................................................................................................................124
Set the Rack ID.........................................................................................................................................................124
Install the Plates on the Sample Rack......................................................................................................................130
Open the Rack Setting Screen..................................................................................................................................134
Set the Plate Type and Needle Stroke from the Top.................................................................................................138
Adjust the Rack Position..........................................................................................................................................142
Connect the System to a Computer................................................................................................................................159
Determine the Amount of Mobile Phase and Rinse Solution Remaining........................................................................160
Lock the System.............................................................................................................................................................162
Clean the Leak Sensor and Leak Tray.............................................................................................................................165
Clean the Automatic Rinsing Kit (if applicable)..............................................................................................................167
LC Column.....................................................................................................................................................................167
Clean the Monitor Screen..............................................................................................................................................168
Clean the Module Surfaces............................................................................................................................................170
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Contents
Storage and Handling....................................................................................................................................................171
6 Troubleshooting...................................................................................................................................172
Hardware Issues.............................................................................................................................................................172
Analysis Issues...............................................................................................................................................................177
Causes of and Countermeasures for Clogging in Flow Lines..........................................................................................182
A Consumables, Options, and Spares....................................................................................................184
Consumables.................................................................................................................................................................184
Sample Vials for Use with the Autosampler.............................................................................................................185
Septum for Use with the Autosampler.....................................................................................................................188
Microtiter Plate........................................................................................................................................................189
Pump Spares..................................................................................................................................................................191
Autosampler Spares.......................................................................................................................................................192
Column Oven Spares......................................................................................................................................................193
Options..........................................................................................................................................................................193
Other..............................................................................................................................................................................193
B Error Messages.....................................................................................................................................195
C Glossary of Symbols............................................................................................................................214
D Glossary of Warnings..........................................................................................................................219
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Contents
This guide describes the basic operation and troubleshooting for the ExionLCTM 100 system . Read this guide
thoroughly before using the product and operate the product in accordance with the instructions in this guide.
This guide provides safety instructions and precautions to make sure that the user operates the system safely.
Follow all Warning and Caution instructions provided in the guide.
Keep this guide for future reference. Make sure that it is accessible to the operator of the system.
Electrical Precautions
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the
covers might cause injury or malfunctioning of the system. The covers need
not be removed for routine maintenance, inspection, or adjustment. Contact
a SCIEX Field Service Employee (FSE) for repairs that require the covers to be
removed.
•Follow required electrical safe work practices.
•Use cable management practices to control electrical cables. This will reduce the chance of a tripping hazard.
AC Mains Supply
Connect the system to a compatible AC mains supply as instructed in this guide.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the
installation of all of the electrical supplies and fixtures, and make sure that
all of the installations adhere to local regulations and safety standards.
WARNING! Electrical Shock Hazard. Make sure that the system can be
disconnected from the mains supply outlet in an emergency. Do not block
the mains supply outlet.
WARNING! Electrical Shock Hazard. Use only the power cables supplied with
the system. Do not use power cables that are not properly rated for the
operation of this system.
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1
Operational Precautions and
Limitations
CAUTION: Potential System Damage. Do not unpack or connect any system components.
The FSE will unpack, connect, and configure the system for the proper operating voltage.
Guidelines:
•Do not connect the wiring in a manner other than that prescribed by the manufacturer.
•Do not rest heavy objects on the power cable.
•Do not bend or pull on the power cable. To disconnect the system, pull on the plug and not the cable.
•Do not route the power cable near heat-generating equipment.
•Do not modify the power cable in any way.
Protective Earth Conductor
The mains supply must include a correctly installed protective earth conductor. The protective earth conductor
must be installed or checked by a qualified electrician before the system is connected.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the
protective earth conductor. Any interruption of the protective earth conductor
creates an electrical shock hazard.
Environmental Precautions
Use qualified personnel for the installation of electrical mains, heating, ventilation, and plumbing supplies and
fixtures. Make sure that all of the installations comply with local bylaws and biohazard regulations. For information
about the required environmental conditions for the system, refer to the Site Planning Guide.
Allow access space around the equipment when setting up the system.
WARNING! Fire Hazard. Do not operate the system in the presence of an open flame,
or in the same room as equipment that could potentially emit sparks.
WARNING! Biohazard. For biohazardous material use, always comply with local
regulations for hazard assessment, control, and handling. This system or any part
is not intended to act as a biological containment.
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Operational Precautions and Limitations
WARNING! Environmental Hazard. Follow established procedures for disposal
of biohazardous, toxic, radioactive, and electronic waste. The customer is
responsible for disposal of hazardous substances, including chemical, waste
oils and electrical components, in accordance with local laws and regulations.
WARNING! Fire Hazard. Do not use flammable sprays (such as hair sprays or
insecticide sprays) near the system. They could ignite and cause a fire.
CAUTION: Potential System Damage. Avoid exposure to corrosive gas and excessive dust.
CAUTION: Potential System Damage. Take precautions to prevent the system from falling
in the event of an earthquake.
Electromagnetic Environment
CAUTION: Potential Wrong Result. Do not use this device in close proximity to sources of
strong electromagnetic (EMC) radiation (for example, unshielded intentional RF sources),
because EMC radiation might interfere with the proper operation and cause a wrong result.
Make sure that a compatible electromagnetic environment for the equipment can be maintained so that the device
will perform as intended.
Decommissioning and Disposal
Before decommissioning, decontaminate the entire system following local regulations.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations. Refer to Storage and Handling.
Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE
to obtain a copy of the form.
Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.
Waste Electrical and Electronic Equipment
Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of
waste, electrical, and electronic equipment (WEEE). To safely dispose of this equipment, contact a local Customer
Service office for complimentary equipment pick-up and recycling.
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Operational Precautions and Limitations
Ventilation Precautions
The venting of fumes and disposal of waste must comply with all of the federal, state, provincial, and local health
and safety regulations. It is the responsibility of the customer to make sure that the air quality is maintained in
compliance with local health and safety regulations.
WARNING! Flammable Chemical Hazard, Biohazard, Ionizing Radiation Hazard,
and Toxic Chemical Hazard. Be sure to use the system in a well-ventilated
laboratory environment in compliance with local regulations and with
appropriate air exchange for the work performed. Solvents used in high
performance liquid chromatography are flammable and toxic.
Chemical Precautions
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
Determine whether decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with the system.
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
WARNING! Fire Hazard. If large amounts of flammable waste solvents are collected,
then take preventative measures to minimize the risk of electrical fire due to static
electricity.
CAUTION: Potential System Damage. Do not submerge the end of the drain tubing in the
waste liquid in the waste container.
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX
Safety Data Sheets can be found at sciex.com/tech-regulatory.
•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
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Operational Precautions and Limitations
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
•Make sure that all exhaust hoses are connected properly and that all connections are functioning as designed.
•Collect all spent liquids and dispose of them as hazardous waste.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
•(Recommended) Use secondary containment trays beneath the solvent bottles and the waste collection container
to capture potential chemical spills.
System Safe Fluids
The following fluids can safely be used with the system.
CAUTION: Potential System Damage. Do not use any other fluid until confirmation is
received from SCIEX that it does not present a hazard. This is not an exhaustive list.
•Organic Solvents
•MS-grade acetonitrile; up to 100%
•MS-grade methanol; up to 100%
•Isopropanol; up to 100%
•HPLC-grade or higher water; up to 100%
•Buffers
•Ammonium acetate; less than 1%
•Ammonium formate; less than 1%
•Acids and Bases
•Formic acid; less than 1%
•Acetic acid; less than 1%
•Trifluoroacetic acid (TFA); less than 1%
•Heptafluorobutyric acid (HFBA); less than 1%
•Ammonia/ammonium hydroxide; less than 1%
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Operational Precautions and Limitations
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