Sequoia LISST-HOLO Bedienungsanleitung


Technical assistance
To obtain technical assistance related to your LISST-HOLO, please
contact your local distributor (a list of our international distributors can
be found on www.SequoiaSci.com) or Sequoia Scientific, Inc. directly.
Please be sure to include the instrument serial number with any
correspondence.
Sequoia Scientific, Inc. Contact Information:
Telephone: +1(425) 641-0944
Email: in[email protected]


Waste Electrical and Electronic Equipment
Smaltimento di apparecchiature elettriche ed elettroniche da rottamare


Table of Contents
1Introduction................................................................................................................................9
2Contents and Technical Specifications.................................................................................11
2.1 General Features.......................................................................................................................11
2.2 Parameters Measured and Derived...........................................................................................11
2.3 Particle Size and Concentration ................................................................................................11
2.4 Technology ................................................................................................................................11
2.5 Mechanical and Electrical..........................................................................................................11
Drawings.............................................................................................................................................12
Bulkhead connector Pin-outs..............................................................................................................13
3Getting Started.........................................................................................................................15
3.1 Introduction ................................................................................................................................15
3.2 Connect cables and power up ...................................................................................................15
3.3 Take a hologram........................................................................................................................17
4Hologram Processing with HOLO_Batch and HOLO_Detail ...............................................21
4.1 Intro............................................................................................................................................21
4.2 Install the hologram processing software ..................................................................................21
4.3 Process holograms using HOLO_Batch and HOLO_Detail ......................................................22
4.4 HOLO_Batch –automated batch processing of multiple holograms.........................................23
.TIFF files output by HOLO_Batch .....................................................................................................32
.CSV files output by HOLO_Batch......................................................................................................32
.MAT file output by HOLO_Batch .......................................................................................................33
.Tiff files of individual slices ................................................................................................................34
Summary of files created by HOLO_Batch.........................................................................................34
4.5 HOLO_Detail –processing of individual holograms..................................................................36
Select Button ......................................................................................................................................36
Advanced Settings..............................................................................................................................36
Set ROI, Regen Kernel and Reconstruct Buttons ..............................................................................37
Clean Stack Slider..............................................................................................................................37
Improfile Button...................................................................................................................................39
Zoom and Reset Buttons....................................................................................................................39
Grid Button..........................................................................................................................................39
Save Image Button.............................................................................................................................40
Measure Button ..................................................................................................................................40
4.6 LISST-HOLO User’s Community ...............................................................................................40
5LISST-Holo Field Deployment ................................................................................................41
5.1 Introduction ................................................................................................................................41
5.2 Programming .............................................................................................................................41
Selecting the start/stop program using the PC...................................................................................43
5.3 Preparing for Deployment..........................................................................................................43
with the EMM installed........................................................................................................................43
without the EMM installed...................................................................................................................43
5.4Wake up / power up LISST-HOLO ............................................................................................44
5.5 Start Data Collection..................................................................................................................44
5.6 Stop Data Collection..................................................................................................................44
5.7 Downloading Raw Holograms ...................................................................................................44
Downloading from EMM .....................................................................................................................44
Downloading and deleting from LISST-HOLO internal drive (EMM not installed) .............................44
5.8 Viewing raw holograms in OpenSeeIt........................................................................................47
5.9 Next Deployment or Powering Down.........................................................................................48
6Reviewing Instrument Info and Other Settings ....................................................................49
6.1 Intro to Other Settings................................................................................................................49
6.2 Home page ................................................................................................................................49

System Status ....................................................................................................................................49
Select program ...................................................................................................................................49
Programming Information...................................................................................................................49
6.3 Images page..............................................................................................................................49
6.4 Log page....................................................................................................................................50
6.5 Configure page ..........................................................................................................................51
6.6 Tools page.................................................................................................................................51
Current conditions ..............................................................................................................................51
Take test image..................................................................................................................................51
Image file name..................................................................................................................................51
Sampling control.................................................................................................................................51
Storage maintenance .........................................................................................................................52
Sleep or reboot...................................................................................................................................52
Power profile.......................................................................................................................................52
Set time ..............................................................................................................................................52
Set time zone......................................................................................................................................52
Set time manually...............................................................................................................................52
Change external trigger polarity .........................................................................................................52
Restore default values........................................................................................................................52
Submit log for analysis .......................................................................................................................53
Generate log file .................................................................................................................................53
Check for updates ..............................................................................................................................53
Network parameters...........................................................................................................................53
Delete all images................................................................................................................................53
Laser...................................................................................................................................................54
Camera brightness, gain, shutter, and power ....................................................................................54
Manual exposure................................................................................................................................54
Command to subsystem.....................................................................................................................54
Watchdog shutdown...........................................................................................................................54
Motherboard log .................................................................................................................................54
6.7 About page ................................................................................................................................54
7Handling, Cleaning, Maintenance and Storage ....................................................................56
7.1 Handling.....................................................................................................................................56
7.2 Cleaning.....................................................................................................................................56
7.3 Maintenance ..............................................................................................................................56
7.4 Storage ......................................................................................................................................56
8Ethernet Communications......................................................................................................58
8.1 Router Use.................................................................................................................................58
Connecting to the router via your network..........................................................................................58
Connecting to the router wirelessly ....................................................................................................58
Updating LISST-HOLO software via the Internet...............................................................................58
8.2 Changing the IP address of the Linksys E1000 router..............................................................58
8.3 Changing the LISST-HOLO IP address.....................................................................................59
9FAQs .........................................................................................................................................61
10 Endcap LED Status Light Indicators .....................................................................................63
11 LISST-HOLO Size Ranges.......................................................................................................65
11.1 Size Ranges based on LISST-100X SPHERICAL particles......................................................65
11.2 Size Ranges based on LISST-100X RANDOMLY shaped particles.........................................67
12 Hologram .PGM File Format Structure ..................................................................................69
13 Warranty ...................................................................................................................................71
14 Index..........................................................................................................................................73

LISST-HOLO User’s Guide 9
1 Introduction
Thank you for purchasing the LISST-HOLO Submersible Digital Holographic Camera!
The intent with this introduction is to get you started using the LISST-HOLO as quickly as possible. Sequoia’s goal with the
LISST-HOLO is to make the user interface so friendly that no manual would be required for normal operation, except as a first-
time introduction to the instrument
The LISST-HOLO was shipped to you in a Pelican case with custom foam padding. Included in the shipping box are the LISST-
HOLO, clamps, stands, External Memory Module with USB cable, Ethernet cable, Wireless Router, Serial Cable with Power
Supply, and a spare 5-pin cable for connecting the LISST-HOLO to alternate power source such as a CTD.
Sequoia Scientific, Inc. is pleased that you have chosen one of our products. Thank you! We strive to make our instruments
rugged, reliable, and simple to operate. If you should have any difficulties or questions regarding this product, please feel free to

10 LISST-HOLO User’s Guide
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