VivoVerse VivoCube+ Bedienungsanleitung

Document Number: VVS-QA-016-02

support@vivoverse.com

vivoCube+ User Manual™

v1.1, Hardware Rev B – August 2023

vivoCube+ User Manual

— 2 —

Disclaimer

This instrucon manual is for the vivoCube+ system. To ensure safety, obtain opmum performance,

and to become suciently familiar with the use of this device, any user of the vivoCube+ should study

this manual thoroughly before operang the vivoCube+. Retain this instrucon manual in an easily

accessible place near the vivoCube+ for future reference. If the equipment is used in a manner not

specied by this manual, the safety of the user and he funconal integrity of the vivoCube+ may be

compromised. Always use the vivoCube+ as outlined in this instrucon manual.

Intended Use

This device has been designed to be used as a pressure source in various roune lab work and research

applicaons. Do not use this device for any purpose other than its intended use. This device is

intended solely for research use and is not to be used for any other purposes including, but not limited

to, unauthorized commercial purposes (including Contract Research, diagnoscs, or manufacture),

military purposes, veterinary or clinical use. Contact vivoVerse for full terms and condions.

Handling Precauons, Maintenance, and Storage

•The vivoCube+ is a precision scienc instrument. Use reasonable care in using, handling,

storing, transporng, and disposing of the vivoCube+ (including by wearing appropriate

protecve equipment). Ensure it is used only by qualied laboratory personnel who have been

trained to use it. Avoid subjecng it to sudden or severe impact.

•Connect any cables or tubing to the vivoCube+ gently.

•Do not use the vivoCube+ where it is subjected to direct sunlight, high temperature, high

humidity, dust, or strong vibraons.

•Do not disassemble any part of the vivoCube+.

•To clean ngerprints, dirt, or stains, use a piece of cleaning paper or ssue moistened with

commercially-available, aqueous, mild cleaning soluon.

oDo not use acetone or alcohol to clean the vivoCube+.

•When disposing of this product, follow any regulaons and rules on the disposal of electronics

set by your organizaon and/or the local government.

Warranty

Visit vivoverse.com/salesterms for limited warranty coverage for the vivoCube+.

whatsoever without the express wrien permission of the author except for the use in brief quotaons

vivoVerse

3303 Northland Dr Suite 308

Ausn, TX, 78731

support@vivoverse.com

vivoCube+ User Manual

— 3 —

User Manual Changelog

Version

Publish Date

Version Notes

v1.0

2022-06-14

First revision of the vivoCube+ manual for Hardware Rev A,

vivoCubeOS v1.0

v1.0.1

2022-08-18

Fixed typos. Added note about inspecng tubing for wear and

cauon that it is connected properly.

v1.1

2023-10-01

Updated to vivoVerse branding. Updated features and

instrucons for vivoCubeOS v1.1.5 and Hardware Rev B.

   
 
 
 
vivoCube+ User Manual 
— 4 — 
© 2023 vivoVerse, Inc. All rights reserved. 
/
Table of Contents 
1. Introducon ........................................................................................................................................... 5
1.1. Specicaons .............................................................................................................................. 5
2. vivoCube+ Basics.................................................................................................................................. 8
2.1. Front Panel................................................................................................................................... 8
2.2. Rear Panel .................................................................................................................................... 9
2.3. Pneumac Connecons..........................................................................................................10 
2.3.1. Using preconnected tubing with luer lock connectors ..............................................10 
2.3.2. Using push-to-connect ngs on the vivoCube+ rear panel ..................................11 
2.4. vivoCube+ Power.........................................................................................................................12 
2.5. vivoCube+ Quality Inspecon...................................................................................................12 
3. User Interface ......................................................................................................................................13 
3.1.  Home....................................................................................................................................13 
3.2.  Manual.................................................................................................................................14 
3.3.  Programs ..............................................................................................................................15 
3.3.1. Channel Selecon Menu..................................................................................................16 
3.3.2. Program Progress Menu ..................................................................................................17 
3.4.  Sengs .................................................................................................................................19 
3.5.  Info ........................................................................................................................................21 
4. Upcoming Features.............................................................................................................................22 
5. Potenal Issues and Troubleshoong .............................................................................................23 
Appendix......................................................................................................................................................25 
A. Using an External Pressure Source...........................................................................................25 
B. Updang vivoCube+ Soware..................................................................................................25 
C. vivoCubeOS changelog...............................................................................................................30 
D. Reporng Bugs and Subming Feature Requests................................................................31 
 
 

vivoCube+ User Manual

— 5 —

1.Introducon

vivoCube+ is a congurable pressure-driven uid controller

designed to drive steady, smooth ow of liquids. The vivoCube+ can

be used with custom-programmed pressure sequences or a library

of preset programs ready for immediate use.

The operang owrates and pressures are specied so that

vivoCube+ can be used not only for C. elegans experiments1 using

vivoVerse vivoChip-2x devices but also for a wide variety of other

microuidic applicaons:

•Organoids and 3D organotypic cultures

•2D organ-on-a-chip models

•Zebrash models

•Drosophila larvae

•Other worm models

•Single cell models

1.1. Specicaons

Table 1 outlines pressure control performance specicaons for the

vivoCube+.

Table 1: vivoCube+ specicaons for pressure control performance.

Performance Specs

Pressure range (built-in pump)*

0 to 10 psi (gauge)

Pressure range (oponal

external pressure source)

0 to 30 psi (gauge)

Sensing accuracy

±0.1 psi

Sensing resoluon

±0.002 psi

Control accuracy

±7% from 0.1 to 0.5 psi

±2% from 0.5 to 1 psi

±1% greater than 1 psi

Control stability

±0.01 psi

Maximum ow rate (built-in

pump)*†

1000 mL/min

1 Every vivoCube+ is pre-

programmed with

patented pressure cycles

developed to immobilize

C. elegans within a

vivoChip-2x for high-

resoluon imaging

* Congurable upon

request

† total ow rate is divided

among the two channels

if operated

simultaneously

vivoCube+ User Manual

— 6 —

The specs outlined in Table 1 are dened as follows:

•Pressure range (built-in pump): the output pressure range

that the pump built into the vivoCube+ can provide at zero

ow rate.

•Pressure range (external pressure source): the range of

pressures that can be connected to the Pext port on the

vivoCube+ and controlled via either pressure channel. Before

using an external pressure source with the vivoCube+, it is

crical to read Appendix A.

•Sensing accuracy: the range within which the average

pressure readings from the vivoCube+ pressure sensors will

fall with respect to a (hypothecal) known pressure value.

•Sensing resoluon: the minimum incremental pressure

change that the pressure sensors built into the vivoCube+

can detect.

•Control accuracy: the range within which the average

pressure readings from the vivoCube+ pressure sensors will

fall with respect to a desired pressure setpoint set by the

user. Reported value is based on a representave load

connected to the vivoCube+; control accuracy is ulmately

applicaon-dependent and will vary.

•Control stability: the range within which any given pressure

reading from the vivoCube+ pressure sensors will fall with

respect to a desired pressure setpoint set by the user.

Reported value is based on a representave load connected

to the vivoCube+; control stability is ulmately applicaon-

dependent and will vary.

•Maximum ow rate (built-in pump): the maximum ow rate

the built-in pump can provide.2

2 vivoCube+ is intended to

be connected to a uid

reservoir to drive uid

ow using air pressure.

vivoCube+ User Manual

— 7 —

Table 2 outlines general specicaons for the vivoCube+.

Table 2: General vivoCube+ specicaons.

General Specs

Supply voltage

12 VDC,

Supply current (minimum)

1 A

Dimensions

250 mm x 155 mm x 54 mm

Weight

820 g

Independent pressure channels

2 (A and B)

Flow media compability

Non-corrosive, non-ionic gases,

such as air

Input pneumac connecon

(oponal)

1/8” inner diameter (ID), 1/4”

outer diameter (OD) tubing

(Pext)

Output pneumac connecons

1/16” ID, 1/8” OD tubing (PA,

PB)

The vivoCube+ requires electrical power to funcon. A power

adapter is provided with interchangeable N-, E-, B-, and A-type

blades shown in Figure 1, covering most power outlets in North

America, Europe, United Kingdom, and Australia, respecvely.3

Figure 1: Diagram of the dierent power blade adapters packaged with

the vivoCube+ system.

3 If you require a dierent

type of power adapter for

your electrical power

supply, please contact

support@vivoverse.com

with your country

informaon.

vivoCube+ User Manual

— 8 —

2.vivoCube+ Basics

This secon consists of a descripon of the various physical

features, controls, and indicators on the vivoCube+.

Each vivoCube+ system includes the following components:

•vivoCube+

•12 VDC, 1 A power supply with interchangeable power

blades

•USB Type-B cable (white)

2.1. Front Panel

A top-down view of the vivoCube+ is depicted in Figure 2, showing

the vivoCube+ front panel. The front panel contains the user

interface/control panel of the vivoCube+.

Figure 2: Annotated top-down view of vivoCube+ (front panel).

1. Screen: high-contrast, monochromac OLED screen that

displays menus and informaon during vivoCube+ operaon.

2. Mulfuncon dial control: primary input control for the user

to operate the vivoCube+. The dial can be rotated either

clockwise or counterclockwise to move the cursor

throughout the menus displayed on the screen. The dial can

vivoCube+ User Manual

— 9 —

also be pressed to select the opon highlighted in white in

the displayed menu.

3. Ring light indicator: LED-illuminated ring that acts as an

addional visual indicator (for example, the ring briey

ashes white whenever the user selects a menu opon).

In general, navigaon through the vivoCube+ graphical user

interface (GUI) is achieved by using the mul-funcon dial control.

Rotate the dial clockwise to advance through a menu or an opon;

rotate the dial counterclockwise to step back through a menu or an

opon. Press the dial to “click” and select the currently highlighted

opon. When certain events occur, the ring light indicator might

ash, change color, or fade in or out as an addional visual

indicator.

In addion to the controls and indicators depicted in Figure 2, the

vivoCube+ contains a speaker, which produces chime sounds under

certain condions. For example, a chime plays when the pump is

powered on or o or when a program commences or completes.

Screen and ring indicator brightness sengs and sound sengs can

be modied in the Sengs menu, described in secon 3.4.

2.2. Rear Panel

A rear view of the vivoCube+ is depicted in Figure 3, showing the

vivoCube+ rear panel. The rear panel contains power, pressure, and

data ports.

Figure 3: Annotated rear view of vivoCube+.

1. Power switch: the rocker switch that powers on or o the

vivoCube+ when power is supplied through the power jack

vivoCube+ User Manual

— 10 —

(Figure 3 item 6). When the top part of the switch is

depressed, a blue LED light within the switch is illuminated,

indicang that the vivoCube+ is powered on.

2. Channel A pressure port (PA): the pressure port controlled

within vivoCube+ soware as pressure channel A. 1/8” OD

tubing can be connected to this port.

3. Channel B pressure port (PB): the pressure port controlled

within vivoCube+ soware as pressure channel B. 1/8” OD

tubing can be connected to this port.

4. USB port: a USB connecon to a desktop computer is

possible with this port using the USB Type-B cable provided

with the vivoCube+. This manner of connecon is required

for vivoCube+ soware updates. More informaon on

soware updates can be found in Appendix B.

5. External pressure source port (Pext): the pressure port that

can oponally be connected to an external pressure source.

1/4” OD tubing can be connected to this port. For more

informaon on how to use an external pressure source with

the vivoCube+, see Appendix A.

6. Power jack: the socket to which the vivoCube+ power supply

(provided with the vivoCube+) should be connected. Ensure

that the power switch is in the o posion before connecng

the power supply to the power jack. Do not use a power

supply other than the one provided with the vivoCube+.

2.3. Pneumac Connecons

This secon outlines methods to connect pneumac tubing to the

vivoCube+. The vivoCube+ outputs controllable air pressure that

can be used to pressurize a liquid reservoir (such as a ported GL45

bole) to drive liquid ow.

2.3.1.Using preconnected tubing with luer lock connectors

The vivoCube+ system includes tubing connected to the Channel A

and Channel B pressure ports at the end of which is a plug/male

luer lock. For convenience and reliability of the uidic connecon, it

is recommended to use a socket/female luer connector to connect

your applicaon to the tubing connected to the Channel A and

Inhaltsverzeichnis

Beliebte Controller Handbücher anderer Marken

Digiplex

Digiplex DGP-848 Betriebsanleitung

YASKAWA

YASKAWA SGM series Bedienungsanleitung

Sinope

Sinope Calypso RM3500ZB Bedienungsanleitung

Isimet

Isimet DLA Series Style 2 Installationshandbuch

LSIS

LSIS sv-ip5a Bedienungsanleitung

Rockwell Automation

Rockwell Automation 1769-L31 Bedienungsanleitung

Airflow

Airflow Uno hab Bedienungsanleitung

ABB

ABB ACS580-01 drives Bedienungsanleitung

Panasonic

Panasonic GM1 Series Bedienungsanleitung

Emerson

Emerson SW93 Bedienungsanleitung

AutoAqua

AutoAqua Smart ATO micro SATO-120P Bedienungsanleitung

Panasonic

Panasonic FP? Positioning Unit RTEX Bedienungsanleitung

Samson

Samson Type 5824 Service-Handbuch

Alpha IP

Alpha IP MIOB 21001 Bedienungsanleitung

Siemens

Siemens OpenAir GMA 1 Series Bedienungsanleitung

Surecom

Surecom SR-629 Bedienungsanleitung

Fishman

Fishman TriplePlay Bedienungsanleitung

Regulus

Regulus SRS1 T Bedienungsanleitung

vivoVerse vivoCube+ Bedienungsanleitung

Beliebte Controller Handbücher anderer Marken