WPI 765LP Bedienungsanleitung
765LP & 765HP
Standard and High Performance Peltier Tissue Bath Temperature
Controllers for use with WPI Vibroslice Models NVSL and NVSLM1
INSTRUCTION MANUAL
Serial No._____________________
061801
World Precision Instruments
www.wpiinc.com
765LP & 765HP
WORLD PRECISION INSTRUMENTS 11
Warnings and Cautions
Spillage — If the cutting lubricant/preserving liquid,
e.g.
physiological saline, is
spilled over the instrument it is important for electrical safety reasons to ensure that
the instrument remains safe to use. To avoid the possibility of electrical shock if a
spillage occurs, the unit should be switched off at the mains electrical outlet and
disconnected before touching the instrument. The instrument should be inspected
and tested if necessary by a suitably qualified technician before it is put into further
use.
This instrument must not be operated unless it is adequately grounded.
Cold Water Supply —
It is essential that the Bath Assembly be connected to an
adequate cold water supply before the unit is operated.
Failsafe devices ensure
that the unit will be shut down, and therefore cannot be damaged, if either the
cooling water flow is omitted by mistake or interrupted, or in the case of use as a
heater, if the water freezes in the heat exchanger.
i
Vibroslice
NVSL
Standard
Peltier Temperature
Controller
765LP
Motorized
Vibroslice
NVSLM1
High Performance
Peltier Temperature
Controller
765HP
Standard tissue baths,
replaced by Peltier baths
765LP & 765HP
WORLD PRECISION INSTRUMENTS 12
Copyright © 2001 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
ii
Contents
Warnings and Cautions ..................................................... i
Introduction ..................................................................... 1
Instrument Description ................................................... 2
Set-up Instructions ........................................................................................... 2
Parts List .................................................................................................. 2
Unpacking ............................................................................................... 2
Returns .................................................................................................... 2
Assembly ................................................................................................. 3
Operating Instructions .................................................... 4
Sequence of Operations .................................................................................. 4
Tissue Bath Assembly ...................................................................................... 4
Heat Sink Modules ........................................................................................... 5
Cold Water Supply ........................................................................................... 5
Temperature Control ......................................................................................... 5
Temperature Setting ......................................................................................... 6
Pre-cooling or heating ...................................................................................... 6
Maintenance .................................................................... 7
Power Supply ................................................................................................... 7
Bath Assembly ................................................................................................. 7
Specifications .................................................................. 8
Replacement Parts .......................................................... 8
Warranty .......................................................................... 9
765LP & 765HP
WORLD PRECISION INSTRUMENTS 1
Introduction
The 765 Peltier Tissue Bath Temperature Controllers are intended for use with WPI
Vibroslice models NVSL (manual) and NVSLM1 (motorized) and allow heating or
cooling of the solution contained in the bath assembly. The 765LP and 765HP
Tissue Bath Assembly replace the standard Vibroslice bath; simply remove one
bath assembly and replace it with the other. The tissue blocks of all three are
identical. The 765LP enables the cooling of physiological fluid and tissue from
normal room temperature to 0°C in approximately 5 to 6 minutes. The higher-
powered 765HP will achieve 0°C in about 4 minutes and has the capacity to go as
low as -10°C. Both units have the capability to heat fluid to +40°C. The advantages
of being able to select the most appropriate temperature for tissue sectioning
include improvement in the slice quality and, at lower temperatures, the ability to
maintain viability of unfixed tissue for longer
in vitro
recordings. Additionally, some
enzyme histochemical techniques give better staining results when sectioned at
low temperatures.
765LP and
765HP Control
Units and Bath
Assemblies.
765LP & 765HP
WORLD PRECISION INSTRUMENTS 2
Instrument Description
The 765 Peltier Tissue Bath Temperature Controller is comprised of a mains
operated Control Unit, a Bath Assembly and Bath Heat Sink Modules. The Control
Unit houses a power supply and temperature control circuitry. The Bath Assembly
has a tissue bath with a stainless steel bottom plate, thermoelectric elements and a
cold water fed heat exchanger.
Note: the 765LP and 765HP baths are NOT interchangeable.
Current from the power supply flows through one side of the thermoelectric
elements that act as heat transfer units. Heat is drawn off, cooling the solution in
the tissue bath. The heat generated by this process is removed by the cool water
supply fed through the heat exchanger. The reverse of this process takes place if
the unit is used to heat the solution in the tissue bath.
Set-up Instructions
Parts List
Standard Unit
Standard Power Supply and Control Unit (part number 765/2LP)
Standard Bath Assembly (part number 765/1LP)
Heat Sink Module (1) (part number 765/TFB)
High Performance Unit
High Performance Supply and Control Unit (part number 765/2HP)
High Performance Bath Assembly (part number 765/1HP)
Heat Sink Modules (2) (part numbers 765/TFB and 765/HS)
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should
be noted on the delivery receipt before signing. Concealed loss or damage should
be reported at once to the carrier and an inspection requested. Please read the
section entitled “Claims and Returns” on the Warranty page of this manual.
Returns
Do not return any goods to WPI without obtaining prior approval and instructions
765LP & 765HP
WORLD PRECISION INSTRUMENTS 3
from our Service Department. Goods returned (unauthorized) by collect freight may
be refused. If a return shipment is necessary, use the original container. If the
original container is not available, use a suitable substitute that is rigid and of
adequate size. Wrap the instrument in paper or plastic surrounded with at least
100 mm (four inches) of shock absorbing material. Please read the section entitled
“Claims and Returns” on the Warranty page of this manual.
Assembly
Power Cable—The power cable is permanently wired to the bath unit and is
connected to the control unit by a plug. The user simply plugs the cable into the
mating socket on the rear of the control unit. This allows for easy cleaning of the
bath unit and for replacement/additional baths.
Tissue Bath Assembly—The Bath Assembly will fit directly onto the WPI
Vibroslice without modification. It has the same volume as the standard WPI Bath
Chamber (VSLM1C) and the tissue holder is identical to the standard WPI
Specimen Holder (VSLM1H). See Fig. 1.
765LP & 765HP
WORLD PRECISION INSTRUMENTS 4
Operating Instructions
Sequence of Operations
The 765 Peltier Tissue Bath Temperature Controllers and Vibroslice are
independent — it does not matter which is turned on first, although it is usual to
stabilize the bath at the desired temperature before trying to cut slices.
Tissue Bath Assembly
The Bath Assembly should be filled with physiological buffer solution at room
temperature or less. The bath Heat Sink Module(s) must be present within the bath
(see Heat Sink Section below). Care should be taken to ensure that the cooling
water is circulating prior to initial start up and during operation, otherwise the unit
will be automatically shut-off. See Cold Water Supply Section below.
Note: The power supply to the thermoelectric elements shuts down if the water
supply is either inadequate or not present. When the supply is resumed, the power
supply is automatically re-connected.
Fig. 1—Tissue
bath assembly
bath assembly
tisue holder
locking screws
tisue holder
assembly
coolant connection ports
(cold water inlet & outlet)
electrical cable
(to power unit)
765LP & 765HP
WORLD PRECISION INSTRUMENTS 5
Heat Sink Modules
These are two stainless steel blocks 48mm long x 8mm wide x 14mm tall. One is a
plain block and the second has an embedded thermistor to provide temperature
feedback. Note that the 765LP unit is supplied with the temperature feedback
block only. The feedback block has a cable protruding from one end. The free
end of the cable plugs into the back of the control unit. If this connection is not
made the unit will not work. Place these blocks in the tissue bath — across the
bath, one at the front and one at the rear. Ideally, the feedback block should be at
the rear to remove the possibility of the cutting blade cutting into the feedback
cable. The heat sink modules perform two functions: They both assist in
temperature transfer through the liquid in the bath (thus speeding up the cooling
rate and promoting a uniform temperature gradient through the liquid) and the
block with the embedded thermistor provides a temperature feedback to the
control unit.
There are two functions of the temperature feedback mechanism: it provides the
user with the facility to request (via the control unit) a specific temperature, and it
allows the control unit to detect the presence of liquid in the bath and shut the
system down in the event of its absence.
Cold Water Supply
It is essential that the Bath Assembly be connected to an adequate cold water
supply before the unit is operated.
Typically, the unit may be connected to a cold
water tap and fed to waste. Either port can be used for “inflow” or “outflow”. The
flow of water through the heat exchanger should be greater than 400 mL/min. A
temperature feedback sensor is built into the Bath Assembly and monitors the
temperature of the heat exchanger.
Caution: In order to reach -10°C, the lowest temperature achievable by the
765HP, iced cooling waterflow is required.
Temperature Control
The unit uses proportional temperature control. This will take the bath temperature
to within 0.5°C at the point of measurement. The temperature feedback sensor is
located in one of the stainless steel heat sink blocks and will only indicate the
765LP & 765HP
WORLD PRECISION INSTRUMENTS 6
temperature at the point of measurement. There will, however, be a small variation
in temperature vertically through the bath. Experience will dictate the best
temperature for any given requirement and ambient temperature.
The underside of the bath base is also in contact with a temperature sensor; a
second temperature sensor is mounted on the cooling chamber. The purpose of
these two sensors is to monitor the presence of and the conditions of the buffer
solution in the bath and the cooling water in the cooling chamber. If either of these
detects any abnormality (
e.g.,
a lack of water flowing through the cooling chamber)
they will cause the control unit to shut down the power until acceptable conditions
are restored.
A third temperature sensor is placed in the bath and monitors the physiological
buffer temperature providing feedback for true temperature display (degrees
Celsius) on the control unit.
Temperature Setting
Tu r ning the display switch to
set (hand symbol) and
adjusting the temperature
control knob to the required
temperature sets the bath
temperature. The actual bath
temperature is displayed
when the display switch is
turned to read (eye symbol).
The unit will heat or cool the
solution in the bath (at the
point of measurement) to
within 0.5°C of the set temperature. It should be noted that once a solution starts to
freeze it might begin to act as a thermal insulator and inhibit further cooling.
Pre-cooling or heating
Pre-cooling or heating times will depend on many factors — ambient air
temperature, bath fluid temperature, cooling water temperature, etc., and are
therefore difficult to predict.
765LP & 765HP
WORLD PRECISION INSTRUMENTS 7
Maintenance
Power Supply
The 765/2 Power supply & control unit contains no user-serviceable parts and
requires no maintenance.
Bath Assembly
Caution: Do not autoclave.
Caution: Do not immerse unit in water.
The Bath Assembly requires cleaning with water after use. All steel, including
stainless steel, will rust if left immersed in saline solution. The effect is more
pronounced as the water bath solution evaporates and the salt concentration
increases. The Bath Assembly and Heat Sink blocks should therefore be regularly
cleaned with water to remove saline concentrates. However, do not immerse the
Bath Assembly in water.
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