Covaris R230 Handbuch
R230 Focused-ultrasonicator
Setup & Instrucon Manual
Instrument and Soware Developed for High-performance Sample Preparaon
using Adapve Focused Acouscs® (AFA®) Energy
For Research Only
Product Name: Revoluon | Model Numbers: R230 | Soware: SonoLab 10
R230 Setup & Instrucon Manual
Covaris
Part Number: 010480 Rev C | August 2020
2R230 Setup & Instrucon Manual
Covaris
Part Number: 010480 Rev C | August 2020
UNIVERSAL PRECAUTIONS
Universal Precauons should be followed on all specimen samples, regardless of whether a sample is known to contain
an infecous agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following
government and clinical standards, or their equivalent in the country of use:
• Centers for Disease Control (CDC), Universal Precauons for Prevenon of Transmission of HIV and Other Bloodborne
Infecons, published 1987, updated 1996
• Clinical and Laboratory Standards Instute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline - Second
Edion, published 2004, ISBN 1-56238-530-5
• Clinical and Laboratory Standards Instute (CLSI), M29-A3 Protecon of Laboratory Workers from Occupaonally
Acquired Infecons; Approved Guideline, Third Edion, published 2005, ISBN 1-56238-5674
• Occupaonal Safety and Health Administraon (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens
• Internaonal Standards Organizaon (ISO) 15190:2003, Medical Laboratories – Requirements for Safety
Trademarks
Patented or patent pending and registered or registraon-pending trademarks of Covaris are protected.
Registered names and trademarks used in this document, even when not specically marked as such, are not to be considered
unprotected by law.
WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries.
Teon is a registered trademark of The DuPont Company.
Informaon subject to change without noce
For Research Use Only (RUO)
Not for use in Diagnosc Procedures
Publicaon P/N 010480
Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applicaons
Copyright © 2020 Covaris, all rights reserved.
Covaris, Inc.
14 Gill St, Unit H
Woburn, Massachuses
01801-1721 USA
Printed in U.S.A.
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Covaris
Part Number: 010480 Rev C | August 2020
Warnings
For the Safety of Operang Personnel:
• Make sure that the equipment is properly grounded. DO NOT operate if it’s not properly grounded.
• The unit is equipped with a power plug appropriate for the desnaon country. DO NOT, under any circumstances, remove the
grounding prong from the power cord.
• DO NOT run an acousc treatment with the sample cover in the OPEN posion or without a water bath - the acousc system
will not work.
• If there is any indicaon that the Safety System is not funconing properly, DO NOT operate the equipment and contact Covaris
immediately.
• If the equipment is used in a manner not specied by the manufacturer, the protecon provided by the equipment may be
impaired.
To Prevent Damage to the Equipment:
• The instruments are designed to operate in ambient laboratory condions e.g., 19 °C to 25 °C (66 °F to 77 °C).
DO NOT operate the instrument in a cold room environment. The system is designed to operate with a water bath and re-
circulang heater/chiller apparatus to control sample temperature.
• NEVER run a method without water in the bath since this could damage the transducer. The instrument is equipped with a
water level sensor to protect the transducer and degassing pump. The system will not allow the degassing pump to operate or
the acousc treatment to start unless an adequate volume of water is detected.
• Dislled or deionized water should be used to ll the water bath.
• Unless the Water Condioning System 3 (WCS) is acvely employed, empty the water bath and wipe it dry EVERY DAY with a
lint-free cloth. DO NOT leave water in the tank for an extended me as there is no water ltraon or water cleaning system
within the apparatus (unless WCS is employed).
• DO NOT employ isopropyl alcohol, ammonia-based or abrasive cleaners in the system, as these will damage the acrylic surfaces.
• Establish a standard of operaon and test the equipment periodically, as described in the maintenance secon of this manual.
CAUTION: All WCS 3 components have pressure rangs above Covaris’ specied maximum operang pressure
which have been extensively tested. However, if plant DI water is connected to the WCS, a catastrophic failure
may result in substanal plant water spillage. It is the user’s responsibility to specify and implement appropriate
containment/alarm schema for their facility.
4R230 Setup & Instrucon Manual
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Part Number: 010480 Rev C | August 2020
Warranty
When used in accordance with wrien instrucon and under normal operang condions, the Covaris instruments are guaranteed
to be free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original delivery by an authorized
representave. Any component proven defecve during the stated period will be repaired free of charge or replaced at the sole
discreon of Covaris, F.O.B., Woburn, Massachuses, U.S.A. provided that the defecve component is returned properly packaged with
all transportaon charges prepaid. The customer is expected to perform basic diagnoscs and component replacement via telephone
support from Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the
customer. A limited warranty as specied may apply to certain components of the equipment.
Warranty Excepons
This warranty is void if failure of the soware or hardware has resulted from accidents, abuse, improper maintenance or repair, or by
misapplicaon from the customer. It is also void if damage is caused by any unauthorized aachments or if modicaons are made to
the equipment. Removing or tampering with the Safety Enclosure will void the warranty, and the customer will assume all liabilies.
This warranty is limited to the original purchaser and is not transferable.
The soware will perform according to the accompanying wrien materials and the medium on which the soware is delivered is
free of defects in materials under normal use and service. The warranty is void if damage has resulted from third party soware not
intended for use with the system.
The high power focused transducer is designed to give maximal mechanical energy output in water. Permanent damage to the
transducer and electronic circuits could result if the transducer is operated without water. Operaon of the system without water in
the water bath voids the warranty.
CONTACT COVARIS, INC. SHOULD YOU HAVE QUESTIONS OR CONCERNS PERTAINING TO THE USE OF OUR EQUIPMENT.
Warranty Services
The purchased equipment is covered by a twelve (12) month warranty which includes all the necessary services and support necessary
so that the customer can successfully operate their equipment. Extended warranes are available at the end of the original 12 month
warranty period.
Services included with the original purchase of the system are:
Technical Support – Ongoing assistance with the operaon or applicaon of the equipment and/or troubleshoong is provided via:
• Telephone
- United States: Tel: +1 781.932.3959 during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,
Eastern Standard Time (EST), Greenwich Mean Time (GMT-05:00)
- Europe: Tel: 44 (0) 845 872 0100, during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Greenwich Mean Time
Parts Replacement – Replacement of parts (excluding consumables) from normal operaon of equipment are provided on a priority
basis. All labor and shipping charges are included. Failure due to accident, abuse, or improper operaon is not covered.
This manual includes maintenance guidelines and troubleshoong ps.
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Covaris
Part Number: 010480 Rev C | August 2020
Table of Contents
1. Introducon ...................................................................................................................................................................................... 6
1.1. Overview of the User Manual ........................................................................................................................................... 6
1.2. The R230 Instrument and the Covaris Process .............................................................................................................. 6
2. Unpacking and Setup ...................................................................................................................................................................... 7
2.1. Unpack and Idenfy Components & Accessories. ......................................................................................................... 7
2.2. Install the On-Deck Module .............................................................................................................................................. 8
3. Geng Started ................................................................................................................................................................................. 17
3.1. Start the System ................................................................................................................................................................... 17
3.2. Start SonoLab ....................................................................................................................................................................... 17
3.3. System Shut-down .............................................................................................................................................................. 18
4. AFA Operaon .................................................................................................................................................................................. 19
5. SonoLab Soware ............................................................................................................................................................................ 20
5.1. The Method Screen ............................................................................................................................................................. 20
5.2. The Schedule Screen ........................................................................................................................................................... 25
5.3. The History Screen .............................................................................................................................................................. 28
5.4. The About Screen ................................................................................................................................................................ 30
5.5. The Admin Screen ................................................................................................................................................................ 30
6. Creang & Eding Treatment Methods ....................................................................................................................................... 36
6.1. Create a New Method ........................................................................................................................................................ 36
6.2. Using the Repeat Funcon ................................................................................................................................................. 42
6.3. Scanning Treatments ........................................................................................................................................................... 45
6.4. Using Drag and Drop with Treatment Steps ....................................................................................................................46
7. System Specicaons ...................................................................................................................................................................... 48
8. Maintenance ..................................................................................................................................................................................... 49
8.1. Cooling Air Intake ................................................................................................................................................................ 49
8.2. Water Bath ............................................................................................................................................................................ 49
8.3. Transducer ............................................................................................................................................................................. 50
8.4. WCS 3.................................................................................................................................................................................... 50
8.5. Safety System ....................................................................................................................................................................... 50
8.6. Degassing System ................................................................................................................................................................ 50
8.7. Cleaning the System ............................................................................................................................................................ 50
9. Troubleshoong ............................................................................................................................................................................... 51
Appendix A: Replace the WCS 3.0 Parculate Filter ..................................................................................................................... 52
6R230 Setup & Instrucon Manual
Covaris
Part Number: 010480 Rev C | August 2020
1.1. Overview of the User Manual
This manual contains the operaon and maintenance instrucons for the Covaris R230 Focused-ultrasonicators. This document
contains informaon essenal to the proper use and care of the equipment.
Should any unforeseen problems occur with the normal operaon of the equipment, contact Covaris Technical Support immediately.
The following denions apply in this manual:
There is also a PDF version of this manual, typically available on the computer desktop. Open the manual by double clicking on its
icon.
1.2. The R230 Instrument and the Covaris Process
The R230 Focused-ultrasonicators are based on Covaris Adapve Focused Acouscs (AFA) technology. These instruments are
designed to provide an integrated tool to be used in a high-volume producon environment. This instrument is ideal for a variety of
applicaons including DNA shearing, mammalian cell lysis, low mass sample extracon from FFPE, ssue, whole blood, compound
screening, bead mixing, and pellet resuspension among other applicaons.
The system is comprised of the:
• Treatment System which delivers the acousc energy to the sample
• Safety System which protects users from inadvertently contacng the high intensity acousc energy
• Computer and Applicaon Soware which provides an interface to control the device via a standard user interface or API
• Water Management System, which consists of a cooler/heater and a UV treatment chamber with parculate lter to maintain
water quality in the instrument bath
The Covaris Process uses adapve focused acousc energy to precisely control cavitaon and acousc streaming within the sample
treatment vessel in a non-contact, isothermal fashion. Focused acousc energy is generated and delivered to individual samples. The
mechanical energy imparted on the sample results in a controlled series of compression and rarefacon events.
1. Introducon
NOTE: Inconvenience if disregarded.
CAUTION: Equipment damage may occur.
WARNING: Personal injury may occur.
7R230 Setup & Instrucon Manual
Covaris
Part Number: 010480 Rev C | August 2020
2.1. Unpack and Idenfy Components & Accessories
The following items are included in the packaging found with the instrument:
• Electronics Box 1.0 (eBox)
• On-Deck R230 Focused-ultrasonicator 1.0 (for placement on the liquid handler deck)
• Water Condioning System 3.0 (WCS 3.0)
2. Unpacking and Setup
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Part Number: 010480 Rev C | August 2020
• Notebook Computer with AC Adapter. Most installaons will transfer Sonolab to the dedicated computer that is connected to
the Liquid Handler.
• Cables
- Computer AC adapter power cord
- Two instrument power cords
- Ethernet cable from eBox to Computer
- Communicaons and power cables between eBox, WCS 3.0, and On-Deck AFA
• Accessories
- The WCS Tubing Kit contains 3 hoses
- The Reservoir Kit contains 2 carboys and 2 hoses
- Mount adapter, sample tubes and racks (varies by applicaon)
- Setup kit
2.2. Install the On-Deck Module
2.2.1. Install the appropriate mounng/locang plate to the liquid handler by following the instrucons provided with the
mounng plate.
2.2.2. Remove the shipping locks from the On-Deck module.
There are 2 bolts that lock the On-Deck moon system in place for shipping. The bolts are accessed from the boom of the
base plate.
• Tilt the On-Deck module up to access the boom of the plate. In the image below, the module is resng on its back
edge with the 2 shipping bolts circled. Be careful to remove only these 2 bolts.
• Lower the On-Deck module so that it is again resng at on the bench.
NOTE: The notebook computer should be available for IQ/OQ, PM and Service. Please DO NOT use this computer for
other purposes.
NOTE: A mount plate must be used for all integraons with liquid handlers.
CAUTION: Shipping locks must be removed before powering up the instrument.
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Part Number: 010480 Rev C | August 2020
2.2.3. Store the shipping bolts on the On-Deck base plate.
• Remove the 5 at head cap screws, 2 from the right, and 3 from the le lower edge of the On-Deck cover. Do not
remove the screws in the inverted “U” shaped cut-outs.
• Li the cover o and set aside.
Remove these bolts only
Remove 5 screws
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Part Number: 010480 Rev C | August 2020
• Thread the two shipping bolts into the holes provided at the le rear of the module’s base plate, near the cable
connectors.
2.2.4. Level the On-Deck module in the liquid handler.
• Place the On-Deck module, without its cover, into the mounng plate installed previously in the liquid handler.
• Perform any nal adjustments required to the mounng plate.
• Level the On-Deck module by adjusng the 4 leveling thumbscrews, as necessary, while monitoring the 2 levels
provided on the base. There is a leveling screw at each corner of the module base. Three of the screws and the le-
to-right level are shown in the image of the module without the cover above. The front-to-back level is on the le side
of the base plate, between the cable and hose connector panels. See image below.
On-Deck Module with outer cover removed
Le-to-Right Level
3 of 4 Leveling Screws
Shipping Bolts
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