Waters 2489 Betriebsanleitung

2489 UV/Visible

Detector

Overview and Maintenance Guide

715004752/Revision A

June 11, 2015, 715004752 Rev. A

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June 11, 2015, 715004752 Rev. A

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General Information

BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE

PUBLISHER.

The information in this document is subject to change without notice and should not

be construed as a commitment by Waters Corporation. Waters Corporation assumes

no responsibility for any errors that may appear in this document. This document is

believed to be complete and accurate at the time of publication. In no event shall

Waters Corporation be liable for incidental or consequential damages in connection

with, or arising from, its use. For the most recent revision of this document, consult

the Waters Web site (waters.com).

Trademarks

ACQUITY, Empower, MassLynx, PIC, “THE SCIENCE OF WHAT’S POSSIBLE.”, and

Waters are registered trademarks of Waters Corporation, and ACQUITY Arc, e-SAT/IN,

FractionLynx, and TaperSlit are trademarks of Waters Corporation.

Tygon is a registered trademark of Saint-Gobain Performance Plastics Corporation.

Other registered trademarks or trademarks are the sole property of their owners.

Customer comments

Waters’ Technical Communications organization invites you to report any errors that

you encounter in this document or to suggest ideas for otherwise improving it. Help

us better understand what you expect from our documentation so that we can

continuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at

[email protected].

June 11, 2015, 715004752 Rev. A

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Contacting Waters

Contact Waters with enhancement requests or technical questions regarding the use,

transportation, removal, or disposal of any Waters product. You can reach us via the

Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose

chemical, biological, or radiological hazards (or any combination thereof). You must

know the potentially hazardous effects of all substances you work with. Always follow

Good Laboratory Practice, and consult your organization’s standard operating

procedures.

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is used to

find out the nature of the potential hazard and any actions which have to be taken.

Considerations specific to the 2489 UV/Visible detector

Power cord replacement hazard

Waters contact information

Contacting medium Information

Internet The Waters Web site includes contact information for

Waters locations worldwide. Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800 252-4752, or fax 508

872 1990.

For other locations worldwide, phone and fax numbers

appear in the Waters Web site.

Conventional mail Waters Corporation

Global Support Services

34 Maple Street

Milford, MA 01757

USA

Warning: To avoid electric shock, use the SVT-type power cord in the United

States and HAR-type (or better) cord in Europe. The main power cord must be

replaced only with one of adequate rating. For information regarding what cord

to use in other countries, contact your local Waters distributor.

June 11, 2015, 715004752 Rev. A

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FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for

compliance, could void the users authority to operate the equipment. This device

complies with Part 15 of the FCC Rules. Operation is subject to the following two

conditions: (1) this device may not cause harmful interference, and (2) this device

must accept any interference received, including interference that may cause

undesired operation.

Electrical power safety notice

Do not position the instrument so that it is difficult to disconnect the power cord.

Equipment misuse notice

If equipment is used in a manner not specified by its manufacturer, protections

against personal injury inherent in the equipment’s design can be rendered

ineffective.

Safety advisories

Consult Appendix A for a comprehensive list of warning advisories and notices.

Operating this instrument

When operating this instrument, follow standard quality-control (QC) procedures and

the guidelines presented in this section.

Applicable symbols

Symbol Definition

Manufacturer

Date of manufacture

Authorized representative of the European Community

Confirms that a manufactured product complies with all

applicable European Community directives

Australia EMC compliant

June 11, 2015, 715004752 Rev. A

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Audience and purpose

This guide is intended for personnel who install, operate, and maintain 2489

UV/Visible (UV/Vis) detectors.

Intended use of the 2489 UV/Visible detector

Waters designed the 2489 UV/Visible detector to analyze and monitor many

compounds.

Calibrating

To calibrate LC systems, follow acceptable calibration methods using at least five

standards to generate a standard curve. The concentration range for standards must

include the entire range of QC samples, typical specimens, and atypical specimens.

Quality control

Routinely run three QC samples that represent subnormal, normal, and above-normal

levels of a compound. If sample trays are the same or very similar, vary the location

of the QC samples in the trays. Ensure that QC sample results fall within an

acceptable range, and evaluate precision from day to day and run to run. Data

collected when QC samples are out of range might not be valid. Do not report these

data until you are certain that the instrument performs satisfactorily.

Confirms that a manufactured product complies with all

applicable United States and Canadian safety

requirements

Consult instructions for use

Alternating current

Electrical and electronic equipment with this symbol may

contain hazardous substances and should not be disposed

of as general waste.

For compliance with the Waste Electrical and Electronic

Equipment Directive (WEEE) 2012/19/EU, contact Waters

Corporation for the correct disposal and recycling

instructions.

Serial number

Part number catalog number

Symbol Definition

5()

June 11, 2015, 715004752 Rev. A

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EMC considerations

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-001.

Cet appareil numérique de la classe A est conforme à la norme NMB-001.

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with IEC CISPR 11 Industrial

Scientific and Medical (ISM) instruments requirements.

Group 1 products apply to intentionally generated and/or used conductively coupled

radio-frequency energy that is necessary for the internal functioning of the

equipment.

Class B products are suitable for use in both commercial and residential locations and

can be directly connected to a low voltage, power-supply network.

EC authorized representative

Waters Corporation

Stamford Avenue

Altrincham Road

Wilmslow SK9 4AX UK

Telephone: +44-161-946-2400

Fax: +44-161-946-2480

Contact: Quality manager

June 11, 2015, 715004752 Rev. A

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June 11, 2015, 715004752 Rev. A

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General Information .................................................................. iii

Trademarks ................................................................................................ iii

Customer comments ................................................................................... iii

Contacting Waters ...................................................................................... iv

Safety considerations .................................................................................. iv

Safety hazard symbol notice.............................................................. iv

Considerations specific to the 2489 UV/Visible detector ......................... iv

FCC radiation emissions notice........................................................... v

Electrical power safety notice............................................................. v

Equipment misuse notice .................................................................. v

Safety advisories ............................................................................. v

Operating this instrument ............................................................................ v

Applicable symbols........................................................................... v

Audience and purpose ...................................................................... vi

Intended use of the 2489 UV/Visible detector ...................................... vi

Calibrating ...................................................................................... vi

Quality control................................................................................. vi

EMC considerations .................................................................................... vii

Canada spectrum management emissions notice................................. vii

ISM Classification: ISM Group 1 Class B ............................................. vii

EC authorized representative ....................................................................... vii

1 Theory and Principles of Operation .......................................... 15

1.1 Detector description .................................................................................. 15

1.2 Principles of operation ............................................................................... 17

1.2.1 Detector optics................................................................................ 17

1.2.2 Wavelength verification and test........................................................ 21

1.3 Operational modes .................................................................................... 22

1.3.1 Single-wavelength mode .................................................................. 22

1.3.2 Dual-wavelength mode..................................................................... 24

1.3.3 Spectrum scanning .......................................................................... 25

1.3.4 Cuvette operations .......................................................................... 26

1.3.5 RatioPlot ........................................................................................ 26

Table of Contents

June 11, 2015, 715004752 Rev. A

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1.3.6 MaxPlot.......................................................................................... 26

1.3.7 Thermal-wander management........................................................... 26

2 Installing the Detector ............................................................. 27

2.1 Before you begin ...................................................................................... 27

2.2 Unpacking and inspecting .......................................................................... 27

2.2.1 Unpacking the detector .................................................................... 27

2.2.2 Inspecting the detector .................................................................... 28

2.3 Selecting a laboratory site ......................................................................... 28

2.4 Stacking system modules .......................................................................... 29

2.5 Connecting to the electricity source ............................................................. 30

2.6 Connecting signal cables ............................................................................ 31

2.6.1 Connecting I/O cables ...................................................................... 32

2.6.2 Connecting signal cables from the rear panel and Ethernet connectors to the

detector......................................................................................... 33

2.6.3 Making Ethernet connections............................................................. 33

2.6.4 Starting a method ........................................................................... 35

2.6.5 Turning the detector lamp on or off .................................................... 36

2.6.6 Connecting the detector to the 2695 separations module ...................... 36

2.6.7 Connecting the detector to a chromatography data workstation using an

e-SAT/IN module ............................................................................ 38

2.6.8 Connecting the detector to a 745/745B/746 data module...................... 41

2.6.9 Connecting the detector to a fraction collector ..................................... 42

2.7 Plumbing the detector ............................................................................... 43

2.7.1 Connecting columns in HPLC systems................................................. 44

2.7.2 Connecting columns in ACQUITY Arc systems ...................................... 44

2.7.3 Assembling the fittings (HPLC only).................................................... 45

2.7.4 Making tubing connections in HPLC systems........................................ 45

2.7.5 Making tubing connections in ACQUITY Arc systems ............................. 45

3 Prepare the Detector ............................................................... 47

3.1 Initializing the detector .............................................................................. 47

3.1.1 Diagnostic test failure ...................................................................... 48

3.1.2 Idle mode....................................................................................... 48

3.2 Using the operator interface ....................................................................... 49

3.2.1 Using the display............................................................................. 49

3.2.2 Using the keypad............................................................................. 51

3.2.3 Navigating the user interface ............................................................ 55

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