Turner AquaFlash Bedienungsanleitung

P/N: 998-8601

Revision: A

January 14, 2016

TURNER DESIGNS

845 W. Maude Ave.

Sunnyvale, CA 94085

Phone: (408) 749-0994

FAX: (408) 749-0998

AquaFlash User’s Manual

Table of Contents

Introduction

1.1

Description

1.2

Pulse Amplitude Modulated (PAM) Fluorometry

1.3

Quick View Diagrams

1.4

Inspection

1.5

General Information, Precautions & Cleaning

Instrument Operation and Calibration

2.1

Button Identification and Function

2.2

Instrument Power Up

2.3

Calibration Check

2.4

Measuring a Sample

2.5

Data Displayed

2.6

Downloading Data

2.7

Installing the Interface/Integration Adaptor Cable

Sample Analysis Guidelines

Measurement Parameters

4.1

Chlorophyll

4.2

Photosynthetic Efficiency

Warranty

5.1

Terms

5.2

Warranty Service

5.3

Out of Warranty Service

Appendixes

Specifications

Warning Messages

Calibrating the AquaFlash

AquaFlash User’s Manual

WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) DIRECTIVE

Turner Designs is in the business of designing and selling products that benefit the well-being of

our environment. Accordingly, we are concerned with preserving the surroundings wherever

our instruments are used and happy to work with customers by complying with the WEEE

Directive to reduce the environmental impact resulting from the use of our products.

WEEE Return Process:

To arrange the return of an end-of-life product, proceed as follows:

If you purchased your instrument through a Turner Designs Distributor please contact your

local representative. They will instruct you where to return the end-of-life product.

If you purchased your instrument directly from Turner Designs please contact Turner

Designs Customer Service

By Phone: 1-408-212-4041 or Toll Free: (877) 316.8049

By Email: Customer Service at [email protected]

Turner Designs will provide a WEEE RMA Number, a Shipping Account Number, and a Ship

to Address. Package and ship the product back to Turner Designs.

The product will be dealt with per Turner Designs’ end-of-life recycling program in an

environmentally friendly way.

AquaFlash User’s Manual

1 Introduction

1.1 Description

The AquaFlash is a handheld active fluorometer that provides estimates for Total

Chlorophyll (µg/L) and photosynthetic efficiency of algae (Yield). This information helps

users rapidly assess the photosynthetic capability of phytoplankton in both oligotrophic and

mesotrophic environments.

1.2 Pulse Amplitude Modulated (PAM) Fluorometry

Pulse amplitude modulation is a process that uses varying light intensities, delivered in a

series of pulses, to look at specific phytoplankton fluorescence characteristics which are

used to directly estimate the photosynthetic efficiency of algae or plant material. There are

two measurement modes, single and multiple turnover. The AquaFlash is a multiple

turnover PAM fluorometer in that one saturating pulse of light is delivered to the sample to

quickly reach the maximum fluorescence value with the interest of looking at how efficient

algae are at photosynthesizing, essentially determining “health” of the organisms. Nutrient

limited or light stressed algae are examples of an “unhealthy” organism.

1.3 Quick View Diagrams

The AquaFlash uses four standard or rechargeable AAA user-replaceable batteries.

Battery panel

O-ring

Battery panel

O-ring

AquaFlash User’s Manual

1.4 Inspection

Upon receiving your instrument, please inspect everything carefully and make sure all

accessories are present. All shipments include:

The AquaFlash inside a storage pouch (batteries installed)

Laminated Quick Start Guide (QSG)

Data download cable and serial to USB adapter cable

USB Flash Drive with User’s Manual, QSG, AquaFlash Software, and serial to

USB cable driver

Optional Accessories available:

Wrist strap P/N 030-8500

10mm x 10mm Square Glass Cuvette (1 ea) P/N 7000-955

Adjustable Solid Secondary Standard P/N 8000-951

AquaFlash Calibration Solution P/N 8600-225

Replacement Download Cable P/N 021-0830

Replacement Storage Pouch P/N 142-8000

Replacement Communication Cable P/N 2300-115

1.5 General Information, Precautions and Cleaning

The plastic battery tab at the back of the AquaFlash must be removed before use.

Grasp it firmly and pull gently to remove it to activate the batteries that power the

AquaFlash.

Use caution around solvents, they may degrade the plastic case of the AquaFlash.

If a sample is accidentally spilled inside the sample compartment, invert the AquaFlash

to drain out the excess liquid. Then wipe the inside area dry with a clean soft towel or

tissue followed by a quick wipe using non-abrasive material, such as Kim Wipes.

If extra cleaning is needed, use a mild detergent to dampen a towel for cleaning.

Although the AquaFlash floats, do not submerge it in water.

Do not expose the AquaFlash to temperatures outside the specified range of

5 to 40 °C or damage may occur to the unit that will not be covered under warranty.

Grasp the plastic tab firmly

and pull gently to remove.

AquaFlash User’s Manual

2. Instrument Operation and Calibration

2.1 Button Identification and Function

CAL –Is used to calibrate the instrument. When selected it will prompt the user to insert

blank. After blank is read, it will prompt user to insert CAL solution. After CAL solution

is read, the screen will display “Calibration Done”.

NOTE: SEE APPENDIX C BEFORE CALIBRATING THE AquaFlash.

CAL VAL –Is used to assign a value to the calibration solution used when calibrating

the AquaFlash. When selected it will prompt the user to set a calibration value from 1 to

50. After the value is set, the user will press enter to save the setting.

READ –Begins the sample analysis procedure detailed in section 2.4.

ON/OFF –Turns the instrument ON or OFF.

ENTER –Allows users to proceed to the next screen/menu.

DATA –When the DATA button is pressed users can choose between either

sending/clearing data or setting date/time. Use the  keys to choose between the two

options; a marker will indicate the selection made.

If SEND/CLEAR DATA is selected, press ENTER and a submenu will display. Users

can choose, using  keys, to either send data to a connected computer, see section

2.6 or clear logged data; a marker will indicate the selection made.

oIf SEND DATA is selected and ENTER is pressed, the instrument will send all logged

data out as an ASCII file, see section 2.6.

oIf CLEAR DATA is selected, the following message will display; “ENTER TO

CONFIRM CLEAR DATA”; when ENTER is pressed all logged data will be deleted.

AquaFlash User’s Manual

If SET DATE/TIME is selected, press ENTER and date and time will display on the

screen as (MM/DD/YYYY) and (hh:mm). Use the  keys to select either parameter; a

marker will indicate selection.

oIf MM/DD/YYYY is selected, press ENTER and you will be allowed to adjust the

month, date, and year using the  keys. Press ENTER to save the set date.

If ESC is pressed prior to saving the set date, the screen is exited and nothing is

saved. Date is logged per sample.

oIf hh:mm is selected, press ENTER and you will be allowed to adjust the hour and

minutes using the  keys. Press ENTER to save the set time. If ESC is

pressed prior to saving the set time, the screen is exited and nothing is saved. Time

is logged per sample.

ESC –Exits to the previous or home screen

CHK STD –Is used to determine instrument drift. “Cal. Check Passed” or “Cal. Check

Failed” will be displayed after measuring an Adjustable Solid Secondary Standard (SSS)

using the CHK STD button indicating whether there is drift in the AquaFlash’s calibration.

SSS is available for purchase as accessory P/N 8000-951.

2.2 Instrument Power Up

Press the ON/OFF button to power on the AquaFlash. After a 5 second warm up, the

AquaFlash is ready for operation. Pressing the ON/OFF button again will turn the unit off or

if left idle on the HOME screen for 3 minutes the unit will automatically turn off to save

battery power.

Note: The AquaFlash will NOT automatically turn off if left idle while running a

sample or if on any screen other than the Home Screen.

HOME SCREEN

The home screen will display the unit’s current date, time, AquaFlash firmware version and

remaining battery power as a percentage. These parameters will be displayed after

powering the instrument on and warm up has completed as well as every time the ESC

button is pressed to exit to the home screen.

Battery power is checked whenever users turn the instrument on using the ON/OFF button.

After the 5 second countdown,

-If the battery power is < 20%, the following warning message will display

“Battery <20%! Press <ENTER>” you can continue making measurements

after pressing ENTER.

MM/DD/YYYY HH:MM

FW1.00 Batt: 100%

AquaFlash User’s Manual

-If the battery power is < 10%, the following message will display “Battery

Low Pwr! Replace Battery!” you will not be able to make any

measurements until batteries are replaced.

2.3 Calibration Check

The AquaFlash is factory calibrated and should remain calibrated for many years.

Calibration can be checked using an Adjustable Solid Secondary Standard (SSS). To check

instrument calibration using a SSS:

1 Press the ON/OFF button to turn the AquaFlash on and wait until the warm up has

completed

2 Press the CAL VAL button to see what the calibration value is set to

3 Obtain an Adjustable Solid Secondary Standard (SSS) and loosen the small black

locking hex screw located on the back of the SSS.

4 Holding the SSS by the tab, insert it with the tab at the back of the sample

compartment and close the sample compartment lid

NOTE: The AquaFlash should be on a flat surface when reading the

Adjustable Solid Secondary Standard (SSS).

5 Press the READ button

a. If the CHL value displayed is lower than the calibration value determined in step

2, adjust the SSS to give you a larger response by rotating the silver hex screw

located at the top of the SSS counterclockwise and re-read the SSS.

b. If the CHL value displayed is higher than the calibration value determined in step

2, adjust the SSS to give a smaller response by rotating the silver hex screw

located at the top of the SSS clockwise and re-read the SSS.

6 Continue adjusting and reading the SSS until the CHL value displayed matches the

calibration value +/-5% determined in step 2.

7 When finished, tighten the locking screw on the back by turning clockwise to lock the

SSS’s response.

8 Insert the SSS into the instrument.

9 Press the CHK STD button.

a. If the setting is valid, the AquaFlash will display “Cal. Check Passed”.

b. If the AquaFlash displays “Cal. Check Failed”, repeat steps 3-9.

The SSS can now be used to periodically check for instrument drift and can be stored in the

side pocket of the AquaFlash storage pouch.

AquaFlash User’s Manual

2.4 Measuring a Sample

NOTE: See Section 3 for Sample Analysis Guidelines before proceeding.

The following materials are required for measuring a sample:

-AquaFlash

-Glass or Quartz Cuvette

-Kim Wipes for wiping the outside of the cuvette

The following procedure is used to measure a sample:

1. Obtain a cuvette and fill it ¾ full with your water sample.

2. Dry and clean all faces of the cuvette using Kim Wipes.

3. Make sure the AquaFlash is on and ready for use. Press READ. You will be prompted

to insert your sample and press READ.

4. Insert the cuvette into your AquaFlash and close the lid.

5. Press READ again and the measurement will begin.

6. The measurement is complete, remove the cuvette and discard the sample.

7. Results will be displayed as shown below:

8. Press the down arrow key to view Fo (minimum), Fm (maximum), and Blank raw

fluorescence values.

9. When finished viewing data, or if ready to run the next sample, press ESC to get to the

Home screen and repeat the sampling procedure.

CHL: 5.25 HH:MM

YLD: 0.54 # 0001

Note: The figure to the left is an example of how results will

be displayed after measuring a sample. CHL is total

chlorophyll, YLD is Yield or photosynthetic efficiency, Time

is displayed as hours and minutes, and the sample number

is displayed as 4 digits representing sample numbers from

1 to 1000.

Fo: 250 Fm: 450

Blank: 50

AquaFlash User’s Manual

2.5 Data Displayed

Data can be viewed on the AquaFlash’s display or downloaded to a computer for viewing.

To view data use the  arrow keys to scroll to a desired sample, sample number will be

displayed in the bottom right corner of the screen. Press the down arrow key to view raw

fluorescence data for that sample.

The following parameters are recorded in the order specified below for a single

measurement and can be downloaded to a computer after they’ve been logged:

Date MM/DD/YYYY

Time hh:mm:ss

Serial Number 86XXXXX

Sample Number 1 - 1000

Chlorophyll 00.01 - 2000, “ND”

Yield 0.01 –0.75, “ND”

Fo Raw minimum fluorescence value

Fm Raw maximum fluorescence value

Blank Value Value recorded during cal for blank

STD Read Value Value recorded during cal for standard

STD Entered Value Standard value set during cal for CAL VAL

Note: “ND” indicates a Non Detect, meaning the sample is well below the lower

detection limit of the instrument.

2.6 Downloading Data

If your computer does not have a serial port available, you will need to install the

Interface/Integration Adaptor Cable (see section 2.7) before proceeding. Once installed

plug the Data Download Cable into the Adaptor Cable and continue with Downloading Data

instructions.

If your computer has a serial port, plug the Data Download Cable into the serial port and

continue with Downloading Data instructions.

The AquaFlash can log a total of 1000 data points which can be downloaded to a computer

using the provided Data Download Cable and Download Utilities Software.

1) Plug the USB drive into your computer

2) Open the Download Utilities Software folder

3) Double click the Setup file

4) Wait for setup to finish installing Download Software

5) Double click to open the Download Software

6) Assign the appropriate COM port by manually typing the designated port number into the

COM port box available

7) Make a connection between the AquaFlash and your computer using the Data Download

Cable

8) Click the Browse button and navigate to a folder or location where the downloaded data

will be saved

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