10x Genomics Chromium X Series Referenzhandbuch

10xgenomics.com

Quick Reference Cards | CG000471 | Rev D

Chromium X Series (X/iX)

For use with:

Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331

Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327

Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332

Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327

Chromium iX & Accessory Kit, 12-Month Warranty, PN-1000328

Chromium iX Accessory Kit, PN-1000323

Chromium iX Accessory Kit, 24-Month Warranty, PN-1000329

Chromium iX Accessory Kit, PN-1000323

Chromium iX/X Refurbished, PN-1000326R

10xgenomics.comQuick Reference Cards | CG000471 | Rev D 2

d. Plug the Chromium X/iX into a properly

grounded outlet of proper voltage.

Ensure power source is grounded before

plugging in the instrument’s power cord.

e. If using an Ethernet cable (not provided),

connect directly to the instrument.

Refer to User Guide (CG) for complete information

Chromium X Series (X/iX)

Step 1: Installation

Location requirements:

• Not in direct sunlight

• Level, sturdy laboratory bench that is not

subject to movement or vibration

• Provides maximum bench space (front to back)

• Allows access to the wall plug and to the

detachable power cord at the rear

• Minimum of 4” (10 cm) clearance around unit

• Minimum of 6” (15 cm) directly in front of the

instrument for movement of the tray door

The tray door is capable of moving an object

in its path, which could fall, creating a hazard.

Heavy Load: Total box weight is 49.9 lb (22.6

kg). Two-person lift required. Use tote bag to

lift the instrument itself (41.4 lb [18.8 kg]).

Unbox

a. Open box according to box label “this side up.”

Remove tape and open. Deep cuts may damage the

Upgrade Kit box (present with upgrade purchase).

b. Remove the Upgrade Kit box (if present) and

packaging material. Lift inner box out and

open according to box label “this side up.”

c. Remove the Chromium iX Accessory Kit

box and packaging material.

d. Lift out tote bag holding the instrument and

place on bench top. Remove instrument from

tote bag. Remove outer packaging materials.

Remove Shipping Lock

a. Grab red sticker on the tray door by the

“pull” tab and pull down to open tray.

Unscrew (by hand) the red metal shipping

lock. Retain shipping lock for future use.

b. Close the tray door.

c. Find universal power cord and plug adaptors

in the Accessory Kit box. Attach correct

country adaptor to the universal adaptor.

DO NOT POWER ON UNTIL READY FOR

FIRST-TIME USE SETUP

Inner Box

Outer Box

Chromium

X Series

Instrument

Chromium X Upgrade Kit

(only present when X upgrade purchased)

Chromium iX

Accessory Kit

10xgenomics.comQuick Reference Cards | CG000471 | Rev D 3

Chromium X Series (X/iX)

Step 2: First-Time Use

The instrument will walk through a ﬁrst-time

setup protocol. Setup requires the following:

• 10x Genomics Account

Onscreen instruction will guide account setup.

Alternatively, visit cloud.10xgenomics.com to create an

account beforehand.

• Instrument serial number and instrument ID

Located onscreen during setup, and also on the label at

the back of the instrument

• A computer with Internet access

Internet connection to the instrument is highly

recommended.

Turning on the system

a. Power on the instrument using the power

switch at the back of the unit.

If using Ethernet, connect the cable to the instrument

Ethernet port.

b. Navigate to register.10xgenomics.com using

the onscreen QR code on a computer or phone

with internet access. Enter the serial number

+ instrument ID. Read and accept the legal

agreement.

c. Update the ﬁrmware.

Internet connection: After registration, the instrument

will automatically search for the latest firmware update.

Confirm data transmission settings for automatic

upload/download.

No Internet connection: Download the latest firmware

update from your 10x Genomics account to the USB

provided in the Accessory Kit. Insert USB into the back

of the instrument. Reference the Firmware Update

Procedure section in the User Guide for more details.

d. Reboot to complete installation. Proceed to

Readiness Test.

Back

Power Supply USB Ports

Ethernet Port

Fan

Front

Touchscreen

Tray

Screen

Refer to the Chromium X Series (X/iX) User Guide (CG000396) for complete information

10xgenomics.comQuick Reference Cards | CG000471 | Rev D 4

Chromium X Series (X/iX)

Step 3: Readiness Test

The readiness test verifies that all systems are working

optimally.

• During ﬁrst-time setup, follow screen

instruction to launch the readiness test.

a. Verify that the shipping/readiness test block is

properly placed on the tray:

• Press the eject button.

• Ensure that the blank gasket is properly

attached.

• Replace the assembled block on the tray.

• Press the eject button again to close the tray.

b. After the test is complete, follow screen

instruction to remove the block with blank

gasket from the tray.

c. Store the assembled block and gasket in a

plastic bag, away from sun-light and extreme

temperature. Retain for future use.

d. A successful readiness test marks the end of

setup. The instrument is now ready for use.

Initiate subsequent readiness tests through the

home screen by loading the shipping/readiness

test block with gasket into the instrument.

Operation Screens

After the readiness test, the instrument is ready for use.

Representative instrument operation screens are shown

below.

Refer to the Chromium X Series (X/iX) User Guide (CG000396) for complete information

Clock

Set up during first-time use.

Access through the menu

option > System/Date & Time

Main Button

Eject shown when

ready for use

Status Icons

Displays successfully-

connected devices.

Menu Options

Press icon to

navigate to that

menu option

10xgenomics.comQuick Reference Cards | CG000471 | Rev D 5

Chromium X Series (X/iX)

Firmware Update Procedure

Step 1: Download and install the update

Procedure with a network connection, auto-download

enabled:

Automatic downloads of updates are enabled after

firmware updates to ensure the instrument is up-to-date

with the latest assay products, features, and bug fixes.

a. A pop-up on the home screen will alert the

user of available updates.

b. Open pop-up and click to install.

Procedure with a network connection, auto-download

disabled:

a. A pop-up on the home screen will alert the

user of available updates.

b. Open pop-up and click to download.

c. Once the update has been downloaded,

install the update.

Procedure with an instrument not connected to the

internet:

a. Visit cloud.10xgenomics.com and log into

an account to which the instrument is

registered.

b. Navigate to the Instruments page using the

top left dropdown button.

c. Download the latest ﬁrmware update onto

a USB drive using the “Download Latest

Firmware” button.

Firmware updates are linked to specific instruments. If

the chosen account is linked to multiple instruments,

select the correct download link for the desired serial

number.

The Chromium X Series instrument is compatible with

VFAT, FAT, and exFAT formatted USBs.

d. Insert USB into the USB port.

e. Onscreen, navigate through the menu to

System > Firmware Update > Update via

USB.

f. Follow the onscreen instructions to update

your instrument using a USB drive.

g. After installation is complete, remove USB

from instrument.

Step 2: Reinitialize the instrument

After installing an update, run a Readiness Test. See

Readiness Test section for further guidance.

a. Retrieve the shipping/readiness test block

with attached blank gasket.

See the Readiness Test section for more information

on the block.

b. Initiate a readiness test through the home

screen by loading the block into the

instrument.

c. After the test is complete, follow screen

instruction to remove the block from the

tray.

d. Store the assembled block and gasket in

a plastic bag, away from sun-light and

extreme temperature. Retain for future use.

e. The instrument is now ready for use.

Contact:

support@xgenomics.com

x Genomics

 Stoneridge Mall Road

Pleasanton, CA  USA

Genomics, is strictly forbidden. Nothing contained herein shall constitute any warranty, express or implied, as to the performance of any products described herein. Any and all

warranties applicable to any products are set forth in the applicable terms and conditions of sale accompanying the purchase of such product. 10x Genomics provides no war-

ranty and hereby disclaims any and all warranties as to the use of any third-party products or protocols described herein. The use of products described herein is subject to

certain restrictions as set forth in the applicable terms and conditions of sale accompanying the purchase of such product. A non-exhaustive list of 10x Genomics’ marks, many of

which are registered in the United States and other countries can be viewed at: www.10xgenomics.com/trademarks. 10x Genomics may refer to the products or services offered

by other companies by their brand name or company name solely for clarity, and does not claim any rights in those third-party marks or names. 10x Genomics products may be

covered by one or more of the patents as indicated at: www.10xgenomics.com/patents. All products and services described herein are intended FOR RESEARCH USE ONLY and

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The use of 10x Genomics products in practicing the methods set forth herein has not been validated by 10x Genomics, and such non-validated use is NOT COVERED BY

10X GENOMICS STANDARD WARRANTY, AND 10X GENOMICS HEREBY DISCLAIMS ANY AND ALL WARRANTIES FOR SUCH USE. Nothing in this document should be construed

as altering, waiving or amending in any manner 10x Genomics terms and conditions of sale for the Chromium Controller or the Chromium Single Cell Controller, consumables or

software, including without limitation such terms and conditions relating to certain use restrictions, limited license, warranty and limitation of liability, and nothing in this docu-

ment shall be deemed to be Documentation, as that term is set forth in such terms and conditions of sale. Nothing in this document shall be construed as any representation by

10x Genomics that it currently or will at any time in the future offer or in any way support any application set forth herein.

10xgenomics.comQuick Reference Cards | CG000471 | Rev D 6

Chromium X Series (X/iX)

Document Revision Summary

Document Number

CG000471 | Rev D

Title

Chromium X Series (X/iX) Quick Reference Cards

Revision

Rev C to Rev D

Revision Date

October 2023

Specific Changes

Step 1: Installation now includes instructions on how to remove the shipping lock, on page 2.

General Changes

The wording for instructions in Step 1: Installation have been made more concise on page 2.

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