Agilent Technologies G1314B Bedienungsanleitung

Agilent Technologies

Agilent 1260 Infinity

Variable Wavelength

Detector

User Manual

Agilent 1260 Infinity VWD User Manual

Notices

2013

No part of this manual may be reproduced

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tronic storage and retrieval or translation

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Manual Part Number

G1314-90013 Rev. C

Edition

11/2013

Printed in Germany

Agilent Technologies

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76337 Waldbronn

This product may be used as a com-

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Otherwise, it is intended only for gen-

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Agilent 1260 Infinity VWD User Manual 3

In This Guide

This manual covers the Agilent 1260 Infinity Variable Wavelength Detectors

•G1314B Agilent 1260 Infinity Variable Wavelength Detector VL

•G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+

1 Introduction to the Variable Wavelength Detector

This chapter gives an introduction to the detector, instrument overview

and internal connectors.

2 Site Requirements and Specifications

This chapter gives information on environmental requirements, physical

and performance specifications.

3 Installing the Detector

This chapter provides information on unpacking, checking on

completeness, stack considerations and installation of the module.

4 Using the Detector

This chapter provides information on how to set up the detector for an

analysis and explains the basic settings.

5 How to optimize the detector

This chapter gives hints on how to select the detector parameters and the

flow cell.

6 Troubleshooting and Diagnostics

Overview about the troubleshooting and diagnostic features.

4 Agilent 1260 Infinity VWD User Manual

In This Guide

7 Error Information

This chapter describes the meaning of detector error messages, and

provides information on probable causes and suggested actions how to

recover from error conditions.

8TestFunctions

This chapter describes the detector’s built in test functions.

9 Maintenance

This chapter provides general information on maintenance and repair of

the detector.

10 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

11 Identifying Cables

This chapter provides information on cables used with the Agilent 1200

Infinity Series modules.

12 Hardware Information

This chapter describes the detector in more detail on hardware and

electronics.

13 Appendix

This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity VWD User Manual 5

Contents

1 Introduction to the Variable Wavelength Detector 9

Introduction to the Detector 10

Optical System Overview 11

System Overview 16

2 Site Requirements and Specifications 19

Site Requirements 20

Physical Specifications 24

Performance Specifications 25

3 Installing the Detector 31

Unpacking the Detector 32

Optimizing the Stack Configuration 34

Installation Information on Leak and Waste Handling 39

Installing the Detector 43

Flow Connections to the Detector 46

4 Using the Detector 49

Leak and Waste Handling 50

Setting up an Analysis 51

Special Settings of the Detector 66

5 How to optimize the detector 73

Optimizing the Detector Performance 74

Match the Flow Cell to the Column 75

Set the Detector Parameters 78

6 Troubleshooting and Diagnostics 79

Overview of the Detector’s Indicators and Test Functions 80

Status Indicators 81

Available Tests versus Interfaces 83

Agilent Lab Advisor Software 84

6 Agilent 1260 Infinity VWD User Manual

Contents

7 Error Information 85

What Are Error Messages 86

General Error Messages 87

Detector Error Messages 94

8 Test Functions 103

Intensity Test 104

Cell Test 106

Wavelength Verification-Calibration 108

ASTM Drift and Noise Test 110

Quick Noise Test 111

Dark Current Test 112

Holmium Oxide Test 114

9 Maintenance 117

Introduction to Maintenance 118

Warnings and Cautions 119

Overview of Maintenance 121

Cleaning the Module 122

Exchanging a Lamp 123

Exchanging a Flow Cell 126

Repairing the Flow Cells 128

Using the Cuvette Holder 130

Correcting Leaks 132

Replacing Leak Handling System Parts 133

Replacing the Interface Board 134

Replacing the Module’s Firmware 135

Agilent 1260 Infinity VWD User Manual 7

Contents

10 Parts and Materials for Maintenance 137

Overview of Maintenance Parts 138

Standard Flow Cell 10 mm / 14 µL 139

Micro Flow Cell, 5 mm / 1 µL (only for support) 140

Micro Flow Cell 3 mm / 2 µL 142

Semi-micro Flow Cell 6 mm / 5 µL 144

High Pressure Flow Cell 10 mm / 14 µL 146

Cuvette Holder 148

Leak Parts 149

Kits 150

11 Identifying Cables 151

Cable Overview 152

Analog Cables 154

Remote Cables 156

BCD Cables 159

CAN/LAN Cables 161

RS-232 Cable Kit 162

External Contact Cable 163

12 Hardware Information 165

Firmware Description 166

Optional Interface Boards 169

Electrical Connections 172

Interfaces 175

Setting the 8-bit Configuration Switch (without On-board) LAN 182

Instrument Layout 187

Early Maintenance Feedback (EMF) 188

8 Agilent 1260 Infinity VWD User Manual

Contents

13 Appendix 191

General Safety Information 192

Batteries Information 195

Radio Interference 196

Sound Emission 197

UV Radiation 198

Solvent Information 199

Declaration of Conformity for HOX2 Filter 201

Agilent Technologies on Internet 202

Agilent 1260 Infinity VWD User Manual

Agilent Technologies

Introduction to the Variable Wavelength

Detector

Introduction to the Detector 10

Optical System Overview 11

Flow Cell 12

G1314B/C Lamp 13

Source Lens Assembly 13

Entrance Slit Assembly 13

Filter Assembly 13

Mirror Assemblies M1 and M2 14

Grating Assembly 14

Beam Splitter Assembly 14

Photo Diodes Assemblies 15

Photo Diode ADC (analog-to-digital converter) 15

System Overview 16

Leak and Waste Handling 16

This chapter gives an introduction to the detector, instrument overview and

internal connectors.

10 Agilent 1260 Infinity VWD User Manual

1Introduction to the Variable Wavelength Detector

Introduction to the Detector

The Agilent 1260 Infinity variable wavelength detector is designed for

highest optical performance, GLP compliance and easy maintenance with:

•data rate up to , see “Peakwidth Settings” on page 71,

•13 Hz for standard HPLC with G1314B VWD VL,

•55 Hz for fast- HPLC G1314C VWD VL+,

•deuterium lamp for highest intensity and lowest detection limit over a

wavelength range of 190 to 600 nm,

•optional flow- cell cartridges (standard (10 mm, 14 µL), high pressure

(10 mm, 14 µL), micro (5 mm, 1 µL), semi- micro (6 mm, 5 µL)) are

available and can be used depending on the application needs,

•easy front access to lamp and flow cell for fast replacement, and

•built- in holmium oxide filter for fast wavelength accuracy verification.

For specifications refer to Table 4 on page 27.

Two version of the Agilent 1260 Infinity variable wavelength detector are

available:

G1314B Agilent 1260 Infinity Variable Wavelength Detector VL

G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+ high data rates for

fast HPLC

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