Agilent Technologies 1260 Infinity Bedienungsanleitung

Agilent 1260 Infinity

Evaporative Light

Scattering Detector

User Manual

Agilent 1260 Infinity ELSD User Manual

Notices

2010

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Manual Part Number

G4218-90002

Edition

06/2010

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Agilent 1260 Infinity ELSD User Manual 3

In This Guide...

This manual is designed to describe the installation; operation,

maintenance and basic troubleshooting of the G4218A Agilent

Evaporative Light Scattering Detector. It includes:

1 Introduction

2 Site Requirements and Specifications

Site requirements and specifications of the Agilent 1260 Infinity

Evaporative Light Scattering Detector

3 Installing the Detector

Installation of the detector.

4 Using the System

This chapter describes how to operate the Low Temperature

Evaporative Light Scattering Detector. It includes information

about starting the unit on a routine basis, collecting data and

shutting down the unit.

5 Optimizing Performance

This chapter shows how to optimize the detector to achieve best

results.

6 Troubleshooting and Diagnostics

This chapter describes a series of activities that should be

performed on a periodic basis to ensure maximum

performance. In addition, this chapter includes a protocol that

can be used to determine the cause of problems that are

observed with the instrument.

7 Maintenance

Introduction to maintenance and repair, instructions on simple,

routine repair procedures

4 Agilent 1260 Infinity ELSD User Manual

8PartsforRepair

Detailed illustrations and lists for identification of parts and

materials for repair

9 The Control Panel

This chapter describes the role of the various controls and

displays on the detector.

10 Appendix

This chapter contains safety information.

Agilent 1260 Infinity ELSD User Manual 5

Contents

1 Introduction 9

The Low Temperature Evaporative Light Scattering Detector 10

Principle of Operation 12

Nebulization 14

Evaporation of the Solvent 17

Detection 17

2 Site Requirements and Specifications 19

Site Requirements 20

Power Considerations 20

Gas Considerations 21

Power Cords 21

Exhaust venting and drain requirements 22

Location of the Detector in the Laboratory 23

Environment 23

Specifications 24

3 Installing the Detector 27

Lifting and Carrying the Module 28

Unpacking the System 30

Installing the Detector 32

Gas Supply 33

Vent the Exhaust Line to a Fume Hood 37

Electrical Connections 38

Additional Options 40

6 Agilent 1260 Infinity ELSD User Manual

Contents

Installing the Nebulizer and Nebulization Chamber Assembly 43

Connecting the Siphon Overflow 46

Connecting the Nebulization Gas to the Nebulizer 47

Connecting the Column 47

Powering Up the Unit 47

4UsingtheSystem49

Preparing the System for Operation 50

Auto-zeroing the Detector 51

Manual Auto-zeroing of the Detector 51

External Auto-zeroing of the Detector 51

Routine Operation of the System 52

Installation Test Procedure 54

Powering Down and Shutting Down the System 56

5 Optimizing Performance 59

Selecting the Optimum Temperature 60

Optimizing the Mobile Phase 62

Sample Pre-Treatment 62

Column Treatment 63

Optimizing the Filter 64

6 Troubleshooting and Diagnostics 65

Basics of Troubleshooting 66

Initial Troubleshooting Activities 67

If there is no response from the system 68

Perform the Noise Tests 69

Specific Detector Troubleshooting 70

Noise test procedures 71

Preliminary Activities 72

Contents

Agilent 1260 Infinity ELSD User Manual 7

Electronic Noise Test 73

Background Noise (Stray Light) Test 74

Solvent Noise Test 75

Column Noise Test 77

Troubleshooting based on diagnostic tests 78

Electronic noise test 78

Stray light test 78

Failed run of standard sample 78

Troubleshooting based on instrument signals 79

No signal is observed 79

Decrease in sensitivity 80

A noisy signal is observed 80

7 Maintenance 81

General inspection 82

Cleaning and Decontamination 83

Cleaning the Detector 83

Decontaminating the detector 83

Removing the nebulization chamber and nebulizer 84

Cleaning the Nebulizer 86

Adjusting stray light settings 89

8PartsforRepair91

Evaporative Light Scattering Detector Parts 92

9 The Control Panel 95

The Control Panel 96

The Digital Display 97

The User Interface 98

8 Agilent 1260 Infinity ELSD User Manual

Contents

10 Appendix 107

Safety Information 108

Solvent Information 112

The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002/96/EC) 113

Lithium Batteries Information 114

Agilent Technologies on Internet 115

Index 117

Agilent 1260 Infinity Evaporative Light Scattering Detector

User Manual

Agilent Technologies

Introduction

The Low Temperature Evaporative Light Scattering Detector 10

Principle of Operation 12

10 Agilent 1260 Infinity ELSD User Manual

1 Introduction

The Low Temperature Evaporative Light Scattering Detector

The Agilent 1260 Infinity Evaporative Light Scattering Detector (Figure 1) is

designed to detect compounds in the eluent from high performance liquid

chromatography (HPLC), micro-HPLC, gel permeation chromatography (GPC)

or counter current chromatography (CCC). It is capable of monitoring eluent

flow rates from 5 µl/min to 5 ml/min. Evaporative light scattering detection is

a universal technique which can detect any non-volatile analyte. Detection

does not depend on the absorbtion of radiation and is not affected by the

absorbtion characteristics of the solvent; thus solvents which absorb UV

radiation can be used.

Figure 1 The Agilent 1260 Infinity

Evaporative Light Scattering Detector

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