Ankom FLEX Bedienungsanleitung

Operator’s Manual

2052 O’Neil Rd, Macedon NY 14502

Telephone: (315) 986-8090

Fax: (315) 986-8091

www.ankom.com

Rev 3/2/2023

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Operator’s Manual 
pg. 1 
Rev 3/2/2023 
 
 
Table of Contents 
Table of Contents...................................................................................................................................................1 
1. Introduction..................................................................................................................................................3 
2. Warranty.......................................................................................................................................................3 
3. Contact Information .....................................................................................................................................3 
4. Instrument Description ................................................................................................................................4 
5. Safety Precautions........................................................................................................................................5 
6. Instrument Installation.................................................................................................................................6 
 Site Requirements and Operating Environment...............................................................................6 
 Unpack Instrument ...........................................................................................................................6 
 Unpack Attachments and Accessories..............................................................................................6 
 Wipe Components ............................................................................................................................7 
 Make Connections ............................................................................................................................8 
 Power On.........................................................................................................................................10 
 Setting Nitrogen Pressures..............................................................................................................11 
 Setting Nitrogen Flow Rate of Evaporation Chamber.....................................................................14 
 Prime Reservoir and Pump Lines ....................................................................................................15 
7. HMI Navigation...........................................................................................................................................21 
 Home Screen...................................................................................................................................21 
 Methods..........................................................................................................................................22 
 Diagnostics......................................................................................................................................23 
 Records and Updates......................................................................................................................25 
 Training and Maintenance..............................................................................................................26 
 ANKOM Products ............................................................................................................................26 
 Who we are....................................................................................................................................26 
8. Starting an Assay ........................................................................................................................................27 
 Confirm Nitrogen Pressure .............................................................................................................27 
 Fill Solution Reservoir Bottles .........................................................................................................27 
 Assemble Digestion Vessels............................................................................................................28 
 Load Method...................................................................................................................................29 
 Install Digestion Vessels and Weigh Sample...................................................................................30 
 Manually Close Digestion Oven Door .............................................................................................33 
 Install the SPE Columns...................................................................................................................33 
 Install Round Bottom Flasks............................................................................................................35 
 Confirm Solution Reservoirs are Full...............................................................................................35 
 Start Run .........................................................................................................................................35 

Operator’s Manual

pg. 2

Rev 3/2/2023

Track Progress.................................................................................................................................36

Abort Run........................................................................................................................................36

8.13 Clean Vessel Tops............................................................................................................................37

8.14 End of Run.......................................................................................................................................39

8.15 Powering Down Instrument............................................................................................................41

9. Adjust Method............................................................................................................................................42

10. Custom Method..........................................................................................................................................43

11. Uploading Methods or Software................................................................................................................44

12. Faults.......................................................................................................................................................45

13. Periodic Maintenance.................................................................................................................................46

Solution Reservoirs and Filters........................................................................................................46

Spray Nozzles in the Digestion Oven...............................................................................................46

Instrument Surfaces........................................................................................................................46

Maintenance Schedule for Parts.....................................................................................................46

14. Troubleshooting .........................................................................................................................................47

15. QC and Calibrations....................................................................................................................................48

Solution Delivery Calibration ..........................................................................................................48

16. Appendix A –Reagents...............................................................................................................................52

Best Practices for Solution Preparation: Total and Crude Fat Analysis ..........................................52

Best Practices for Solution Preparation: Vitamin & Cholesterol Analysis ......................................52

17. Appendix B –FLEX Maintenance: Rinsing Slide Valve................................................................................53

Operator’s Manual

pg. 3

Rev 3/2/2023

1. Introduction

Congratulations on your purchase of the ANKOMFLEX Analyte Extractor (FLEX). We are confident that this product will effectively

serve your needs.

The FLEX is designed to simplify fat-soluble vitamin, cholesterol, crude fat, and total (hydrolysis) fat determinations. Supporting

up to four samples at a time, the system will automatically complete digestion (saponification or hydrolysis), solid phase

extraction (SPE), and evaporation of solvent in about two to three hours. By automating these labor-intensive procedures,

accuracy and precision is improved, chemical exposure is limited, and hands-on labor is reduced. This manual will provide you

with details that will help you operate the FLEX efficiently and effectively for precise and accurate results.

ANKOM Technology designs, manufactures, and markets analytical instruments and support products to laboratories around

the world in the food, feed, agricultural, bioenergy, and environmental industries. Our instruments allow for the analysis of

fat-soluble vitamins, cholesterol, dietary fiber, crude and detergent fiber, crude and total fat, digestibility, microbial

fermentation (anaerobic or aerobic) and more.

ANKOM Technology is committed to total customer satisfaction, designing every product based on a thorough assessment of

customer needs.

2. Warranty

ANKOM Technology warrants the ANKOMFLEX Analyte Extractor against any defects in workmanship or material for one year

after the original date of purchase. This warranty does not include damage to the instrument resulting from neglect or misuse.

During the warranty period, should any failure result from defects in workmanship or materials, ANKOM Technology will, at

its discretion, repair or replace the instrument free of charge. Extended warranties are available upon request.

3. Contact Information

We are committed to supporting you and value your feedback.

For any questions or suggestions regarding your instrument, please contact us at:

For Sales Support: s[email protected] or https://www.ankom.com/contact-us

For Technical Support: www.ankom.com/contact/technical-services

For Analytical Support: www.ankom.com/contact/analytical-services

Telephone: (315) 986-8090

Fax: (315) 986-8091

NOTE: Please review the entire manual before you begin operating this product.

Operator’s Manual

pg. 4

Rev 3/2/2023

4. Instrument Description

The ANKOMFLEX Analyte Extractor simplifies fat-soluble vitamin, cholesterol, crude, and total (hydrolysis) fat determinations

and other possible applications. This fully automated system integrates all the steps involved in these analyses in one

instrument. Supporting up to four samples at one time, the system will automatically complete digestion (saponification or

hydrolysis), solid phase extraction and evaporation of the solvent. The system has four solution reservoirs which can be filled

with various chemical solutions as defined by method requirements (e.g. ethanol, hexane, HCl, KOH, water). The FLEX’s

software allows for method development and customization.

Digestion vessels have been designed to allow for sample to be weighed directly into the vessels, thereby eliminating transfer

error. Filtration takes place without user intervention, further reducing technician labor. A nitrogen atmosphere, positive flow,

and low temperature evaporation protects sensitive analytes against oxidation and optimizes solvent evaporation. Exhaust

lines can be connected to an exhaust system therefore eliminating the need to position the entire instrument in a ventilation

hood. Once an assay is complete, the isolated analyte is ready for gravimetric analysis (fat analysis) or reconstitution for

quantitation by HPLC or GC (vitamin, cholesterol, fatty acid profile analysis).

Evaporation

Chamber

Digestion Oven

Human Machine

Interface (HMI)

Touchscreen

Reservoir Door

and Tray

SPE Columns

(Vitamin Column

Pictured)

Reservoir Bottles

Round Bottom Flasks

Digestion Vessels

Operator’s Manual

pg. 5

Rev 3/2/2023

5. Safety Precautions

Hot Surfaces –Do NOT open the Digestion Oven during operation. The

interior surfaces can reach 100°C (212°F). Failure to observe this caution may

result in burns.

Hazardous Voltages –Do NOT operate the instrument with the back of the

main electrical cabinet removed. Hazardous voltages are present during

operation. Failure to observe this caution may result in electrical shock or

electrocution.

Hazardous Materials –Follow SDS warnings and recommendations for all

materials to be used in this instrument, for example: Ethanol, Hexane, and SPE

sorbent. Follow both local and federal regulations for vent hood requirements

when operating this instrument. Failure to observe this caution may be

hazardous to your health.

WARNING: Attempts to override safety features or to use this instrument in a

manner not specified by ANKOM Technology voids the warranty and may result

in serious injury or even death. This system is designed to meet and/or exceed

the applicable standards of CE and CSA.

The power switch must be in the OFF position before plugging the instrument

power cord into the power source.

Operator’s Manual

pg. 6

Rev 3/2/2023

6. Instrument Installation

Site Requirements and Operating Environment

To install and operate the ANKOMFLEX Analyte Extractor you will need the following:

•Adequate Power: 100V−120V ~ 50/60Hz 8A

220V−240V ~ 50/60Hz 8A

•Must have ability to connect to an exhaust system

•Bench space that can accommodate 77.5 cm (30.5”) W x 81.3 cm (32”) H x 45.7 cm (18”) D and a weight of 54.4 kg

(120 lbs.)

•Nitrogen Supply Requirements: a. N2 purity, Industrial Grade (Grade 4.8) which is 99.998% or purer

b. N2 Flowrate of 10LPM at 6.9 bar minimum

c. Supply N2 pressure 5.5-6.9 bar (80-100 psi)

•Fitting adapter to connect nitrogen supply to the ¼” (6.35mm) outside diameter

•Black polyurethane nitrogen supply tubing that ANKOM supplies

•Ambient Temperature Range: 19°−30°C (66°-86°F)

Unpack Instrument

The instrument shipping container consists of three separate cardboard pieces: a top, a bottom, and a sleeve that forms the

body of the container. Remove the top and the sleeve of the instrument shipping container. Lift the instrument from the

shipping container bottom and place it on a surface that is firm and level. The instrument needs to be near a nitrogen supply

and within range to connect exhaust lines to a ventilation hood or exhaust system. The instrument must not be subjected to

excessive shock, vibration, dirt, moisture, oil, or other fluids.

Unpack Attachments and Accessories

Within the shipping container is a cardboard box containing attachments, accessories, and a tool kit. Open the box and verify

that the following items are present. The tool kit will have a separate packing list included.

Digestion Vessel Glass with port

(6 Qty)

part # FLEX55

Round Bottom Flask (6 Qty)

part # 9364

Vessel Bottom Plugs (12 Qty)

part # FLEX72

Vessel Bottom Assembly (6 Qty)

part # FLEX54

Magnetic Cross Stir Bar (6 Qty)

part # 9415

FLEX Tool Kit

Rubber Cleaning Strip (1 Qty)

part # 9479

Black Nitrogen Supply Tubing (1 Qty)

part # 8216

3mm Tee Handle

Hex Wrench

14mm Open

End Wrench

90 Degree

Angled Pick

1/8” Hex Driver

Hex Wrench Set

Operator’s Manual

pg. 7

Rev 3/2/2023

500 ml Solution Reservoir Bottle

(3 Qty)

part # 5605

2000 ml Solution Reservoir Bottle

(1 Qty)

part # 9365

Waste Bottle Assembly

(1 Qty)

part # FLEX49

Sorbent Disposal Bags

(20 Qty)

part # FLEX-SDB

FLEX Support Items

The following support items can be purchased separately.

Item

ANKOM Part #

Vitamin & Cholesterol SPE Column

FLEX-SPE-01

Total Fat SPE Column

FLEX-SPE-02

Column Top

FLEX-9001

Vitamin & Cholesterol Filter

FLEX-VF

Total Fat Filter

FLEX-FF

Sorbent Disposal Bag

FLEX-SDB

FLEX Maintenance Kit

FLEX66

Wipe Components

6.4.1. Wipe the vessel bases, the spray nozzles and O-rings of the digestion vessel tops and the column top adaptors

with a towel wetted with water. Refer to FLEX Service Procedure FLS011.

Including 10 ft Exhaust Tubes

(2 Qty)

Operator’s Manual

pg. 8

Rev 3/2/2023

Make Connections

6.5.1. Connect waste bottle assembly. If your laboratory would like to connect the instrument to its own waste

reservoir, be sure to adhere to the standards below.

•Connect the shorter opaque tube on the waste bottle assembly to the vessel vent and waste port on the back

of the instrument. The instrument cannot drain uphill; it must drain by gravity. This means that the waste

reservoir cannot be higher than the waste port. This scenario would create a backflow to the instrument.

•Connect one of the 3 m exhaust tubes to the globe vent port on the back of the instrument.

•Connect the second 3 m exhaust tube to the waste assembly exhaust line connection.

•Vent the exhaust tubes to an exhaust system. The exhaust system should pull a slight vacuum so that solvent

fumes are properly removed from the FLEX during evaporation. These connections must never be closed or

restricted during operation of the instrument.

•Screw on the cap to the waste reservoir.

•Confirm all connections.It is important the waste reservoir is vented and

does not build pressure.

•All tubes from the instrument must be free of bends and kinks. If the

tubing is kinked, the instrument will not be able to drain properly and

will cause damage to the instrument.

Vessel Vent and

Waste port

Globe Vent

Exhaust line

connection

Waste Reservoir

Exhaust Tubes

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