Bertin Technologies Cryolys Evolution Bedienungsanleitung

Document reference: CLYS-800-DU001-A

Original Instructions

Cryolys®is a registered trademark of Bertin Technologies.

For research use only

Document reference: CLYS-800-DU002-A

Translated instructions from the French original version

Cryolys® is a registered trademark of Bertin Technologies.

For Research purposes only

2WWW.BERTIN-INSTRUMENTS.COM

Restricted User License:

By purchasing or using the Product, the user accepts the terms and conditions of

this License in full.

The purchase or use of the Product confers on the User the non-transferable right

to use the Product for Research purposes only. The term Research denotes any

scientific research programs conducted directly by the User for their own

purposes; the User renounces the right to use the Product for the purposes of

developing a treatment or diagnostic method as well as any use of the Product

for Commercial purposes.

The term "Commercial" includes in particular use of the Product or its

Components, (i) for manufacture, (ii) for the provision of services, information or

data to a Third Party, (e.g. paid sample testing), (iii) for resale, whether within the

context of Research or not, (iv) for quality control or providing quality assurance

for the manufacturer of a commercialized product.

The User is not authorized to modify, resell or transfer the Product or its

components.

3WWW.BERTIN-INSTRUMENTS.COM

Legal Information:

Bertin Technologies and/or its subsidiaries deny any liability either explicit or

implicit and particularly but not exclusively for commercial or individual

warranties linked to this document.

Except where otherwise provided for by law, neither Bertin Technologies nor its

subsidiaries can be held liable, either in contractual or criminal law, and in terms

of a guarantee or under any other regime that may be, for any damage in

whatever form which may occur particularly but not exclusively as a result of the

use of this document.

Neither Bertin Technologies, nor its employees, can be held liable for losses or

costs resulting from its use.

The information contained in this document is provided solely for use with this

Bertin Technologies product under the terms and conditions of the Restricted

User License. Bertin Technologies is not liable for any use of this information when

applied to other products.

Bertin Technologies reserves the right to modify this document and make any

changes at any time without prior notice.

This liability waiver clause is written and interpreted according to French law. Any

legal action relating to the content of this clause is the exclusive jurisdiction of the

Courts of Law in Paris, France.

This document is protected and contains information which is the property of

Bertin Instruments. The Bertin Instruments, Precellys® Evolution and Cryolys®

Evolution logos are registered trademarks of Bertin Technologies.

4WWW.BERTIN-INSTRUMENTS.COM

Table of Contents

............................................................................................................................................1

1Introduction..................................................................................................................5

1.1 Safety instructions and recommendations...................................................5

1.2 Warranty..............................................................................................................9

2Description of the Cryolys®Evolution ....................................................................10

2.1 Description of the equipment.......................................................................10

2.2 Specifications...................................................................................................11

2.3 Generating cold air.........................................................................................12

3Installing the Cryolys®Evolution..............................................................................14

3.1 Unpacking and installation............................................................................14

3.2 Packaging and transport...............................................................................19

3.3 Storage..............................................................................................................20

4Use...............................................................................................................................21

4.1 Description of user interface..........................................................................21

4.2 Starting temperature-controlled homogenization....................................22

5Cleaning and decontamination............................................................................26

5.1 Recommendations..........................................................................................26

5.2 Decontamination protocol............................................................................26

6Support and documentation .................................................................................27

6.1 Frequently asked questions ...........................................................................27

6.2 Troubleshooting................................................................................................28

6.3 Support..............................................................................................................29

6.4 Disposal..............................................................................................................29

7Appendix: Example of CE Declaration.................................................................30

8Appendix: Electrical diagram.................................................................................31

5WWW.BERTIN-INSTRUMENTS.COM

1INTRODUCTION

Thank you for purchasing a Cryolys®Evolution by Bertin Instruments.

The Cryolys®Evolution is a cooling system specifically designed for the Precellys®

Evolution. Based on the use of cooled atmospheric air through the sublimation of

dry ice, it provides optimal protection for temperature-sensitive molecules such

as proteins, small RNAs or even particular chemical compounds by maintaining a

temperature between 0 and 10°C. The Cryolys®Evolution is the solution for

preserving the molecules in your samples and therefore for obtaining the best

possible results.

This user manual includes all the information you need to use and maintain your

Cryolys®Evolution. The technical specifications of this product, along with all the

information contained in this manual may be modified at any time without prior

notice.

1.1 Safety instructions and recommendations

Read this user manual carefully before using your Cryolys®Evolution.

If you are in any doubt about the safety of the device, please contact us by

email at this address: sample-prep@bertin-instruments.com or contact your local

distributor, whose address is listed on our website: Bertin Instruments.

Bertin Technologies cannot be held liable for any damage or injuries caused by

using this device in a manner other than that described in this manual.

6WWW.BERTIN-INSTRUMENTS.COM

Reference labels are attached to the device's components. Users must comply

with the information printed thereon.

1.1.1 Risk of electric shock

As the Cryolys®Evolution is connected electrically to the rear panel of the

Precellys® Evolution, usage precautions and recommendations against electric

shocks are as described in the Precellys® Evolution User Manual (02520-800-

DU003-B).

1.1.2 Biological risks

Always wear the necessary personal protection equipment (PPE) when cleaning

any parts soiled by biological samples, for example inside the Precellys®lid.

Note: Refer to the Precellys®Evolution manual (02520-800-DU003-B) as well as to

the procedures currently in place in your laboratory

Eye and face protection

Hand and skin protection

7WWW.BERTIN-INSTRUMENTS.COM

1.1.3 Risks linked to dry ice

Dry ice (cardice) is made from an accumulation of carbon dioxide in a solid

state with a temperature of -80°C.

It sublimates in air into CO2, a colorless and odorless gas. The principal risks linked

to the handling of this product are asphyxia and burns. In order to protect

yourself against projectiles and the risk of asphyxia, the user must wear protective

gear for the eyes, face and skin. Store and handle dry ice in a ventilated area.

CAUTION! Ensure you are in possession of the safety data sheet covering the risks

and precautions linked to the use of dry ice (handling using protective gloves,

transport and storage under particular conditions, etc.).

1.1.4 Mechanical risks

The lid is liable to self-closure. We recommend keeping it in the open position

with your hand during all operations requiring it to be opened. If not, we

recommend closing it.

When opening the lid, please be aware of the risk of pinching between the

hinge and the rear of the Precellys®Evolution.

Risk of burns: Dry ice at -80°C

Risk of asphyxia

RISK OF

PINCHING

8WWW.BERTIN-INSTRUMENTS.COM

1.1.5 Special Safety Symbol

Comply with the safety instructions associated with this symbol.

1.1.6 Recommendations

Any use of this device other than that described in this manual can alter its

external protection and damage its internal components.

We recommend that you:

-Do not install unauthorized components or accessories; this can alter the

protection afforded by the device and void its warranty.

-Do not drop the device: the Cryolys®Evolution module must always sit

atop the Precellys®Evolution or on a horizontal surface, or its

components may become damaged.

-Dot not handle the device if it is fully or partially dismounted or if it is

damaged.

-Do not invert the connections as this could irreparably damage the

connectors.

-Do not plug the Cryolys®Evolution in to a power supply other than the

one supplied by Bertin Instruments.

-Do not obstruct the fan.

-ONLY use sticks of dry ice (do not use nitrogen or H2O ice).

-Do not fill the dry ice container beyond its limit (see "4. How to Use")

-Do not ship the device if the container contains dry ice.

-Do not ship the device in anything other than the original packaging.

9WWW.BERTIN-INSTRUMENTS.COM

1.2 Warranty

The manufacturer's warranty is limited to one (1) year. This warranty is valid from

the date the equipment is registered on our website: (www.bertin-

instruments.com). This covers failure or malfunction of any of the components in

the device where it has been used in compliance with the recommendations

contained in this manual.

The warranty does not apply in the following cases:

- Use or maintenance which does not comply with the instructions contained in

this manual.

- Opening, repair or modification of the device by unauthorized persons.

- Alteration or removal of the label on which the device's serial number is printed.

Manufacturer:

Bertin Technologies

Parc d’activité du Pas du Lac

10bis Avenue Ampère

78180 MONTIGNY-LEBRETONNEUX

FRANCE

10 WWW.BERTIN-INSTRUMENTS.COM

2DESCRIPTION OF THE CRYOLYS®EVOLUTION

The Cryolys®Evolution is a device designed to keep biological samples in the

Precellys®Evolution at a temperature between 0°C and 10°C to avoid any

degradation linked to a temperature increase during homogenization.

The Cryolys®Evolution allows you to:

-Keep the sample temperature below 10°C throughout the

homogenization process.

-Control the temperature from the Precellys®Evolution’s screen.

-Protect temperature-sensitive molecules from degradation.

2.1 Description of the equipment

Andere Handbücher für Cryolys Evolution

Inhaltsverzeichnis

Andere Bertin Technologies Laborausrüstung Handbücher

Bertin Technologies

Bertin Technologies PRECELLYS 24 Touch Bedienungsanleitung

Bertin Technologies

Bertin Technologies PRECELLYS 24 Bedienungsanleitung

Bertin Technologies

Bertin Technologies CORIOLIS COMPACT Bedienungsanleitung

Bertin Technologies

Bertin Technologies PRECELLYS Evolution Touch Bedienungsanleitung

Bertin Technologies

Bertin Technologies CORIOLIS Compact Bedienungsanleitung

Bertin Technologies

Bertin Technologies Coriolis m Bedienungsanleitung

Bertin Technologies

Bertin Technologies Cryolys Evolution Bedienungsanleitung

Bertin Technologies

Bertin Technologies Coriolis m Bedienungsanleitung

Bertin Technologies

Bertin Technologies PRECELLYS 24 Bedienungsanleitung

Beliebte Laborausrüstung Handbücher anderer Marken

Qiagen

Qiagen DML 3000 Bedienungsanleitung

Queensgate

Queensgate NANOSCAN OP400 Programmierhandbuch

Conviron

Conviron GEN1000-GE Bedienungsanleitung

Parr Instrument

Parr Instrument 4560 Bedienungsanleitung

IKA

IKA IC control Bedienungsanleitung

Integra

Integra DOSE IT Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5800 ICP-OES Bedienungsanleitung

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Bedienungsanleitung

NI PXI-5422 Handbuch

Collomix

Collomix Aqix Bedienungsanleitung

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Bedienungsanleitung

Ocean Insight

Ocean Insight FLAME-NIR+ Bedienungsanleitung

Parker

Parker ALIGN-MG-NA Bedienungsanleitung

BD 644787 Bedienungsanleitung

DENTAURUM

DENTAURUM Compact Megaplus Bedienungsanleitung

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Bedienungsanleitung

VWR

VWR SAS Super IAQ Bedienungsanleitung

illumina

illumina MiSeqDx Bedienungsanleitung