Biosan CH-100 Bedienungsanleitung

Contents

About this edition of the operating manual...............................................................2

Safety precautions ...................................................................................................3

General information..................................................................................................4

Getting started..........................................................................................................5

Operation .................................................................................................................7

Calibration..............................................................................................................10

Specification...........................................................................................................12

Maintenance...........................................................................................................13

Warranty and Claims. Registration.........................................................................14

EU Declaration of conformity..................................................................................15

About this edition of the operating manual

The manual applies to following model and versions of heating/cooling system:

CH-100 versions V.7A01, V.7A02 and V.7A03

CH 3-150 versions V.2AD and V.2AE

Safety precautions

The following symbols mean:

Caution! Make sure you have fully read and understood the present Manual

before using the equipment. Please pay special attention to sections

marked by this symbol.

Caution! Surfaces can become hot or cold during use.

GENERAL SAFETY

Use only as specified in the Operating Manual provided.

Protect the unit from shocks or falling.

Store and transport the unit at ambient temperatures between -20°C and +60°C and

maximum relative humidity of 80%.

After transportation or storage, keep the unit under room temperature for 2-3 h before

connecting to electric circuit.

Before using any cleaning or decontamination methods except those recommended

by the manufacturer, check with the manufacturer that the proposed method will not

damage the equipment.

Do not make modifications to the design of the unit.

ELECTRICAL SAFETY

Connect only to external power supply (CH-100) or electric circuit (CH 3-150) with

voltage corresponding to that on the serial number label.

Use only the external power supply (CH-100) provided with the unit.

Do not use an ungrounded power socket or an ungrounded extension lead.

Ensure that the switch and the plug are easily accessible during use.

If liquid penetrates into the unit, disconnect it from electric circuit and have it checked

by a repair and maintenance technician.

Disconnect the unit from electric circuit before moving.

Do not operate the unit in premises where condensation can form. Operating

conditions of the unit are defined in the Specification section.

DURING OPERATION

Use only tubes of standard size.

Do not check the temperature by touch. Use a thermometer.

(For model CH 3-150) Remove the blocks only using the included extractor tool.

Do not operate the unit in environments with aggressive or explosive chemical

mixtures. Please contact manufacturer for possibility of unit operation in specific

atmosphere.

Do not operate the unit if it is faulty or has been installed incorrectly.

Do not use outside laboratory rooms.

Do not leave the operating unit unattended.

BIOLOGICAL SAFETY

It is the user’s responsibility to carry out appropriate decontamination if hazardous

material is spilt on or penetrates into the equipment.

General information

CH-100 and Combitherm-2 CH 3-150, dry-block thermostats with heating and cooling

function, are designed to thermally stabilize materials at a wide range of temperatures. Built-in

Peltier elements allow fast and precise cooling, and heating plates –heating of the materials

according to used methods.

Model CH-100 with built-in blocks (on request) is intended for micro quantities of

reagents, in volume range from 0.5 to 2.0 ml. Temperature setting range is from -10°C to

+100°C. Model features exceptional temperature maintenance stability and uniformity, as well

as high heating and cooling speed.

Model Combitherm-2 CH 3-150 with changeable blocks is designed for increased

versatility. Temperature setting range is from -3°C to +20°C for cooling block and from +25°C

to +150°C for heating block. Combitherm-2 consists of two independent modules for

interchangeable blocks,cooling and heating,combined ina single unit. Control panel isdivided

in two parts for separate control of cooling and heating blocks. Blocks are controlled

independently, with possibility of setting up to 16 custom programs for different temperature

and thermostating time. Seven thermoblocks are available, for different size and quantity of

tubes. Models can be used in:

Molecular and cell biology, for nucleic acid extraction and analysis.

Protein analysis (denaturation).

Biochemistry, for analysis of enzyme processes.

Chemical laboratories, in matter composition analysis.

Food and environmental investigation laboratories, in composition analysis of food

and cosmetics.

Depending on the model, different quantities of microtubes of different volumes can

be placed in the thermostat. Table 1. Block capacity, by model

Model and block

Capacity

Tube types

Model CH-100

CH-1

20 / 12

0.5 / 1.5 ml Eppendorf-type microtubes

CH-2

1.5 ml Eppendorf-type microtubes

CH-3

2 ml Eppendorf-type microtubes

Model CH 3-150

B23-1.5

1.5 ml Eppendorf-type microtubes

B18-12

Ø12 mm tubes

B10-13

Ø13 mm tubes

B10-16

Ø16 mm tubes

B6-25

Ø25 mm tubes

B5-29

Ø29 mm flasks

B2-50

Ø50 mm beakers

Getting started

4.1. Unpacking.

Remove packing materials carefully and retain them for future shipment or storage of

the unit. Examine the unit carefully for any damage incurred during transit. The

warranty does not cover in-transit damage. Warranty covers only the units

transported in the original package.

4.2. Complete set. Package contains:

4.2.1. CH 3-150 Combitherm-2

- CH 3-150 Combitherm-2 heating/cooling dry block system .............................1 pce.

- Transparent block lids......................................................................................2 pcs.

- Spare fuse (inside fuse holder) .......................................................................1 pce.

- Power cable .....................................................................................................1 pce.

- Operating manual, declaration of conformity ..................................................1 copy

- B23-1.5 block ........................................................................................on request

- B18-12 block .........................................................................................on request

- B10-13 block .........................................................................................on request

- B10-16 block .........................................................................................on request

- B6-25 block ...........................................................................................on request

- B5-29 block ...........................................................................................on request

- B2-50 block ...........................................................................................on request

- Extractor tool ...................................................................... 1 pce. with each block





4.2.2. CH-100

- CH-100 heating/cooling dry-block system........................................................1 pce.

- Transparent block lid........................................................................................1 pce.

- External power supply......................................................................................1 pce.

- Power cable .....................................................................................................1 pce.

- Operating manual, Certificate..........................................................................1 copy

4.3. Setup.

- Place the unit upon even horizontal non-flammable surface at least 20 cm away

from any flammable materials;

- Do not place any objects 40 cm behind the unit to ensure unimpeded air circulation;

- Remove protective film from the display;

- (Model CH 3-150) Plug the power cable into the socket on the rear side of the unit,

and position the unit with easy access to the power switch and plug.

- (Model CH-100) Connect the external power supply and the power cable. Plug the

external power supply into the socket on the rear side of the unit and position the

unit with easy access to the power switch and plug.

4.4. Heating or cooling block installation and replacement (model CH 3-150).

Caution! Heating/cooling block surface may become very hot or cold during

the operation. Avoid touching the block or heating/cooling surfaces.

Change the blocks only at room temperature.

- Use the extractor tool for removing blocks. Screw the tool into a threaded hole in

the middle of a heating/cooling block and lift it out. Unscrew the tool.

- Ensure that the heater bed and the block are clean in order to ensure good thermal

contact between unit and the block, resulting in optimal temperature control

performance.

Operation

Recommendations during operation

Please check the tubes before using, be sure that tubes are heat resistant. Don’t heat

the tubes over the melting point of the material they are made of. Remember that

thin-walled tubes have a higher thermoconducting factor.

Microtube caps can open under the action of high temperature (> 85°C), thus causing

sample volume shrinkage or potential health risk when working with infected material.

To prevent such cases, use microtubes with cap lock of Safe-Lock®type.

Do not fill tubes more than 3-5 mm over the level they are immersed in the heat block.

5.1. Working with model CH-100.

Fig. 1. Control panel of CH-100

5.1.1. Connect the external power supply to a grounded power socket. Switch on (position I)

the power switch on the rear panel of the unit.

5.1.2. The unit will turn on and the following readouts will be shown on the display:

- In the upper line Set: previously set time and temperature parameters;

- In the lower line Actual: operation status (S –stopped), timer indication STOP and

current temperature.

5.1.3. Temperature setting. Use the ▲ and ▼ T (ºC) keys (fig. 1/5) to set the required

temperature. Values are shown on display (upper line, fig. 1/3). Temperature incre-

ment is 0.1ºC. Pressing the key down for more than 2 s makes the values change

quicker.

5.1.4. Press the RUN/STOP T (ºC) key (fig. 1/8) to start heating or cooling. The corres-

ponding operation status is indicated on the display, Hfor heating, Cfor cooling

(fig. 1/1). Current temperature value is shown on display (lower line, fig. 1/5).

5.1.5. To stop the heating or cooling, press the RUN/STOP T (ºC) key again. It takes a few

moments before the heating or cooling process stops and the operation mode

indicator shows S, for stopped.

5.1.6. The set temperature can be changed during operation without stopping the process.

5.1.7. After thermal stabilisation of the unit (i.e. after set and current temperatures equalize,

fig. 1/3), open the block lid, place samples and close the lid.

5.1.8. Timer setting. The unit is equipped with an independent timer for convenient control

over the samples heating/cooling time.

5.1.9. Use the ▲ and ▼ TIME keys (fig. 1/4) to set the required time period. Values are

shown on display (upper line, fig. 1/2). Time increment is 1 minute. Pressing the key

down for more than 2 s makes the values change quicker.

5.1.10.Press the RUN/STOP TIME key (fig. 1/7) once to start the timer. The elapsed time is

indicated in the lower line of the display (fig.1/2). When the set time is reached, the

timer stops and a sound signal is heard.

Caution! After the set time elapses and the timer stops, the device does not

stop the heating or cooling process. Press the RUN/STOP T (ºC) key

(fig. 1/7) manually to stop the heating or cooling.

5.1.11.The timer can be stopped before the set time elapses if required by pressing the

RUN/STOP TIME key. Pressing the key again restarts the timer.

5.1.12.The set time value can be changed during operation without stopping the timer.

5.1.13.After finishing the operation, turn off (position O) the unit switching off the power

switch on the rear panel. Disconnect the external power supply from electric circuit.

5.2. Working with model CH 3-150.

Fig. 2. Control panel of CH 3-150

5.2.1. Connect the power cord to a grounded power socket and switch on (position I) the

power switch located on the rear panel of the unit.

5.2.2. The unit will switch on and the following readouts will be shown on the display:

- In the upper line Set p.: previously set time and temperature parameters;

- In the lower line Actual p.: program indication STOP and current temperature.

Caution! Temperature starts changing automatically after switching on

according to selected settings. Time of temperature stabilization

depends on the initial temperature.

5.2.3. The unit has 16 cooling and 16 heating programs for temperature and time setup.

One cooling and three heating programs are factory pre-set. The programs have

following settings:

Program

Temperature

Time, hh:mm

Program

Temperature

Time, hh:mm

Cooling [P1]

-3 °C