3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: 724-282-0745 • E-mail: [email protected] • Web: www.bioptechs.com 3560 Beck Road
• Butler
, P
A
16002
• V
oice: 724-282-7145
• Fax: 724-282-0745
• E-mail:
[email protected] • W
eb: www
.bioptechs.com
The alarm may sound as a result of the following conditions:
• Perfusion rate exceeds the rate which temperature stability can be maintained. To correct, press the red reset button
as soon as the alarm sounds. This will reset the logic back to cold start up mode. Setpoint temperature will return
within 15-20 seconds.
• An interruption in AC line power may cause the logic levels to change state.
• Physical displacement of the thermistor from the microaqueduct surface.
Note: The thermistor that regulates the chamber frame is sealed internally eliminating accidental displacement. Therefore,
there is no need for an alarm on the chamber frame circuit.
Chamber Loading
Your chamber has been inspected prior to packaging and is ready to use when you receive it. The chamber is shipped assembled
and should be opened using the following procedure.
Rotate the large knurled ring in the base of the chamber clockwise until it stops. This will release and disengage the chamber
top. Hold the white portion of the chamber while simultaneously (using thumb and forenger) rotating CCW the two smaller
knurled nuts located on either side of the black electrical contact unit. This will remove the black portion which contains sensitive
electronics so that they can remain dry. You now have the basic components of the chamber separated. Continue with the following
directions:
Directions for Closed System Use: (see drawing for part identication)
• Attach perfusion tubing to the inlet and outlet ports of the chamber. Bioptechs recommends 1/16” Tygon #2275
tubing for best biocompatibility and ease of use. See perfusion drawing.
• Turn the top of the chamber (white) upside-down. Place the upper gasket (.75mm thick) into the recess while
aligning the perfusion clearance holes with the perfusion tubes.
• Place the microaqueduct slide on the upper gasket so that it is aligned with perfusion tubes (grooved side up).
• Place the lower gasket (0.1mm - 1.0mm thick) onto the microaqueduct slide and press around the perimeter creating
a seal.
• Perfuse some media through the perfusion port producing a bead on the surface of the slide to displace air trapped in
the line.
• Lower the coverslip with cells onto the bead until it is resting on the gasket.
• Place the closure assembly on top of this stack while aligning the black electrical connector with the oval slot in the
chamber top. Maintain a gentle pressure while turning the chamber over so that it is right side up then can observe
as the four paws engage the top through the depressions about the perimeter. Turn the large knurled ring counter-
clockwise until tight. This will symmetrically tighten and seal the chamber.
• For applications involving rapid perfusion, a drop of immersion oil can be placed at the point where the base of the
chamber meets the coverslip in the exposed aperture of the chamber. This drop will be drawn in by capillary action
and enhance thermal conductivity to the coverslip. Place a light lm of immersion oil on the at surface of the
surface probe to enhance thermal conductivity.
• Place the black power/sensor connector back on the two threaded posts and symmetrically rotate CW (using thumb
and forenger) the two knurled nuts onto the post. Make sure the spring wires make contact with the bus bars on the
surface of the microaqueduct slide.
• It is important that the at side of the surface probe is making uniform contact with the microaqueduct slide because
the feedback loop that controls the microaqueduct temperature is reliant on this thermal contact point.
• Plug the FCS2 Chamber into the controller (6 Pin mini DIN).
• The display can be switched between Set, Slide and Chamber at any time to monitor temperature without interfering
with the regulation. If the Chamber was preheated it will come up to temperature in about 1 minute, otherwise it
will take longer.
• The Chamber should now be mounted onto the microscope adapter in the stage.
• The vertical portion of the drain tubing which extends down from the stage to your waste receptacle will create a
siphon and form negative pressure in the chamber. This negative pressure will cause the coverslip to ex. This can
be eliminated by breaking the siphon at a point equal to the height of the specimen with a “T” tting.
