Bright Instruments 8000 Bedienungsanleitung

Instruments

8000 Retracting

Sledge Microtome

Manual

Bright Instruments 2

Contents

SAFETY WARNINGS 3

Low Temperature 3

Knives / Blades 4

SAFETY INFORMATION 5

Consumer Protection 5

Mechanical 5

Operation 5

Accessories 5

Product Safety Suggestions 6

Decontamination Certicates 6

WARRANTY 7

1 INTRODUCTION 8

1.1 Receipt of Product 8

1.1.1 Receipt and unpacking 8

1.1.2 Receipt 8

1.1.3 Unpacking 8

1.2 Assembly & Installation 8

1.2.1 Positioning 8

2OPERATING INSTRUCTIONS 9

2.1 Operating handle (3) 9

2.2 Thickness control (5) 9

2.3 Coarse advance/trim control (4) 9

2.4 Specimen holder 9

2.5 Knife clamps 9

2.6 Knife guards 9

2.7 Side pillars [Fig.1 13 & 16] 9

3 OPERATION 10

3.1 Operating handle (3) 10

3.2 Thickness control (5) 10

3.3 Coarse advance/trim control (4) 10

3.4 Specimen holder 11

3.5 Knife clamps 11

4. MAINTENANCE 12

4.1 Cleaning 12

4.2 Lubrication 12

4.3 Decontamination 12

4.4 Removing the Sledge 12

5 SPECIFICATION 13

6 ACCESSORIES & CONSUMABLES 14

MATERIAL SAFETY DATA SHEET 16

Microtome Lubrication oil 17

REFERENCES & DRAWINGS 19

Figure 1. Front & Side View of Microtome 20

Figure 2. Bottom View of Microtome 21

Figure 3. Knife Holder 22

Figure 4. Setting Blade Angle 23

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SAFETY WARNING

Low temperatures are present in this

equipment. Extreme care should be taken.

DO NOT let bare skin come into contact

with metal surfaces.

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SAFETY WARNING

Extremely sharp knives/blades.

Use knife/blade guards at all times.

Use correct tools for removal and insertion of knives/blades.

DO NOT leave knives/blades laying around.

Place knives/blades not in use, into box/wallet provided.

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Safety Information

CONSUMER PROTECTION

The Consumer Protection Act 1987 Part 1.

refers to Product Liability. This legislation was

issued as a direct result of an EC Directive to

all member states and has been in force with

eﬀect from 1 March 1988.

The Bright Instrument Company Limited,

ever mindful of the need to ensure that their

products are not subject to misuse and/or

incorrect handling, have made it their aim to

communicate any possible dangers to their

customers.

Whilst the Bright Instrument Company Limited

markets products manufactured to the highest

safety standards, it is in the interest of the

purchaser that he is aware of the resultant

dangers of misuse and/or incorrect handling

of these products.

Your attention is therefore drawn to the

following precautions:

MECHANICAL

MICROTOME KNIVES/BLADES:

The microtome knife/blade can be a hazard

in the laboratory. Personnel should be made

aware of the dangers and observe the

following warnings:

a. DO NOT leave the microtome unattended

with an exposed knife/blade in position

Remove the knife/blade, or cover with the

guards or visor provided.

b. DO NOT leave unboxed knives/blades lying

around. Place knives/blades that are not

in use in their boxes or packets.

c. DO NOT carry knives/blades unless secure

in the box or packet provided.

d. DO NOT clean the knife/blade along its

length. Wipe the knife from the back edge to

the cutting edge.

e. REMEMBER that even used knives and

blades are dangerous. The are still sharp

and may have been used to cut potentially

infectious specimens.

f. DISPOSE of used knives and blades with

the same care as other sharp objects. On

no account should used knives or blades be

placed in waste bins.

OPERATION

When placing object holders in the vice,

when orientating, manipulating or in any way

placing ﬁngers in a position above the knife/

blade edge – ensure that knife guards are

in position.

ACCESSORIES

Fluids supplied as accessories with Bright

instruments, such as Cryospray 134 and

microtome oil are strictly for laboratory

use only.

They should not be taken by mouth and

precautions aﬀorded to other laboratory

chemicals should be adhered to. Please refer

to the material safety data information,

towards the back of this instruction manual

for further details.

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PRODUCT SAFETY INFORMATION

All Bright Instrument Company Limited

personnel are encouraged to make suggests

regarding product safety. We also welcome

such suggestions from our customers.

They may be submitted by completing the

appropriate (Safety) section of the Quality

Survey Record Form supplied with all Bright

instruments, or alternatively, by letter,

telephone, fax or email. All communications

should be direct to our Quality Assurance

Department and will be acknowledged.

DECONTAMINATION CERTIFICATES

IMPORTANT If the instrument or any part

of it is to be returned to Bright Instrument

Company Limited, a decontamination

certiﬁcate must accompany it, and please

note the following:

a. If the instrument or any part of it has been

exposed to or been in contact with potential

pathogenic or radioactive material, it is

essential that it be decontaminated.

b. Set procedures are laid down by the Health

and Safety Executive for decontamination.

For the avoidance of doubt, we ask that

instruments or parts returned to us should be

accompanied by a completed decontamination

certiﬁcate. A copy of this can be found at

the back of this instruction manual and we

suggest you use a photocopy of this when

returning parts. Alternatively we would be

pleased to either post or fax you another

copy should you require.

c. Should the instrument or any part of it be

received in a condition that Bright Instrument

Company Limited, consider to be a potential

biological hazard, the instrument or part will

be returned un-repaired at the expense of

the customer.

d. Customers declarations must indicate that

the package contains ‘British Returned Goods’.

Failure to do so will involve customs duty

payable by us, which will be invoiced to

the sender.

Safety Information Cont.

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Warranty

The Warranty relating to the instrument is

outlined in our Terms and Conditions of Sale

paragraph 6.

i. The Seller ‘s products are carefully inspected

and submitted to its standard tests.

ii. The Seller warrants all its products to be

free from defects in workmanship and

materials under normal conditions of use and

service provided always:

a. That if any of the goods so manufactured

is alleged to be defective in workmanship

and material and is returned carriage paid,

and protected against damage in transit to

the Seller’s works at Huntingdon within 12

months from the date of despatch and if

after examination by the Seller that goods

or part of them are found to be so defective

then the Seller will repair or replace them free

of charge and will return them to the Buyer,

carriage paid.

b. Where any part of the goods manufactured

by the Seller is repaired or replaced under

the terms of the foregoing warranty, such

warranty shall thereafter be limited to a

period of six months from the date when

the goods shall have been re-delivered

to the Buyer.

c. This warranty does not apply to any defects

caused by wear and tear, incorrect installation

abnormal conditions of working, accident,

misuse or neglect.

d. That save as in this clause herein before

expressed, the Seller shall not be under any

liability for negligence or otherwise in respect

of defects in goods delivered or for any injury,

damage or loss resulting from such defects

and the Seller’s liability under this clause shall

be in lieu of any warranty or condition implied

by law as to the quality or ﬁtness for any

particular purpose of such goods.

e. This warranty is expressly in lieu of all

other warranties, guarantees or liabilities

expressed or implied by any of the Seller’s

Representatives or Agents.

Please see our separate Product Warranty

sheet for deliveries to the mainland UK.

WARNING: Before proceeding to Operating

Instructions, ensure you are familiar with

the contents of the pages marked ‘Safety

Information’. This instrument must only be

used by competent persons.

Bright Instruments 8

1. Introduction

1.1 RECEIPT OF PRODUCT

1.1.1 RECEIPT AND UNPACKING

This instrument received a ﬁnal test and

inspection prior to despatch from the factory.

The following instructions are given for the

re-assembly of the instrument, adjustments

and its correct use. If the instrument is

received before preparations for installation

are completed, it should be stored in a clean,

dry place and not exposed to dirty or damp

conditions.

1.1.2 RECEIPT

Immediately upon receipt of the instrument,

make a careful examination for evidence of

damage encountered in transit. If any damage

is found or suspected, notify both the carrier

and Bright Instrument Company Limited.

1.1.3 PACKING

All packing must be carefully removed and

parts checked against the enclosed packing

list. If any damage or discrepancy is noted,

please inform our agent/distributor, or

bright Instrument Company Limited direct,

immediately. At least two people are required

during the unpacking. When removing the

main instrument from the packaging, grip the

instrument on the underside (one person to

each side) and lift out the microtome. Do not

lift by the sledge or attempt to separate the

sledge from the casting within the case as

damage can result.

1.2 ASSEMBLY & INSTALLATION

1.2.1 POSITIONING

The instrument should be positioned on a

level, sturdy surface at a height convenient

for the operator. It may be used with its long

axis parallel to the bench, at right angles or

obliquely. Ensure it has been positioned away

from direct, hot sunlight and is in a location

completely free from draughts.

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2. Operating Instructions

On receipt of your new Bright instrument,

please refer to section 1.1 (Receipt and

Unpacking) and section 1.2 (Assembly and

Installation).

As part of its policy of continual improvement,

Bright Instrument Company Limited, reserves

the right to incorporate changes, or make

additions to, without prior notice. There

may, therefore, be minor details diﬀerences

between the information in this manual and

your instrument. These diﬀerences will not

aﬀect the safety and use of the instrument.

Refer to Fig. 1.

2.1 OPERATING HANDLE [FIG. 1.3]

Semi automatic advance – when pushed

towards the knife the sledge will move

along the runners and will also advance the

specimen the selected amount (thickness).

When pulled back the specimen retraction

mechanism is activated.

2.2 THICKNESS CONTROL [FIG. 1.5]

A knob, calibrated in microns, is used to

select the thickness of sections to be cut from

1µm – 40µm, in 1µm steps. Always set the

thickness by turning the knob anticlockwise.

If the thickness has to be reduced then turn

clockwise past the required thickness, then

anticlockwise.

2.3 COARSE ADVANCE/TRIM CONTROL

Quickly raises or lowers the specimen 450µm

per turn. As this control is always engaged, it

can be used at any time.

2.4 SPECIMEN HOLDER

Specimens are clamped in the vice [7] or

alternative holder if ﬁtted. The specimen

holder can be raised and lowered after

releasing the clamp lever [21].

2.5 KNIFE CLAMPS

Two screws, turned by a lever [10], are used to

ﬁx the knife in the knife clamp and the knife

clamp in the knife block [9]. The cutting angle

of the knife is adjustable from 0-40º. A scale

is provided on the knife block. Slew angles of

the knife can be obtained by loosening the

knife block clamp levers [12] and the knife

clamp levers [10] and moving knife to slew

angle required.

2.6 KNIFE GUARDS

Each knife guard [1] can be moved to the

central position to cover the working area

of the knife, or to the outer position while

section cutting. The black knobs secure the

guards in the chosen position.

2.7 SIDE PILLARS (FIG. 1.13 & 1.16)

In normal use, these pillars slope inwards

slightly. For large area sections they can be

unbolted from the base, swapped left to right

and reﬁtted, so that they slop outwards, so

providing more space.

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3. Operation

The microtome should be installed on a sturdy

bench at a height convenient for the operator.

It may be used with its long axis parallel to

the bench, at right angles or obliquely.

There are many variations of the preparation

procedure according to the type of specimen

to be sectioned, the size of the specimen and

how the specimen is prepared.

The following general procedures can be

applied to all uses:

Refer to Fig. 1.

3.1 FITTING THE OBJECT HOLDER

Pull the sledge away from the knife holders.

Secure the required object holder by placing

it in the holder in the sledge slide [19]. Set to

correct height and lock by tightening clamp

lever [21].

Note that the clamp lever has a ratchet with

its movement limited to prevent the lever

being left in positions where it could impact

on other parts during sectioning.

3.2 FITTING AND ADJUSTING THE KNIFE

NB: The term ‘knife’ used here also includes

disposable blade holder.

a. Slacken the knife clamp levers (10) just

enough to let the knife slide into the knife

clamps from the side.

b. Insert the knife, taking care to avoid

touching the edge against the metalwork.

c. Check that the knife lies ﬂat on the pad

pieces in the knife clamps and that the heel

of the knife is not caught up on the slot in the

knife clamps.

d. Screw the knife clamp levers down just

suﬃciently to steady the knife in position.

e. Slacken the knife block clamp levers [12].

Set the knife holders to the desired slew

angle, ensure the knife clamps still cover

the ends of the knife. Set the knife to the

desired cutting angle and tighten the knife

clamping levers.

f. Check that the knife is still at the desired

slew angles and tighten the knife block clamp

levers.

NB: To ensure that the knife clamps are

correctly aligned in the holders,

the knife block clamp levers must

always be slackened before the knife

clamping levers are tightened.

3.3 TRIMMING THE SPECIMEN

Fit the specimen securely into the appropriate

specimen holder on the microtome.

To trim excess material from the surface of

the specimen block:

a. First move the sledge towards the knife and

adjust the specimen height using the coarse

advance so that the specimen is just below

the knife.

b. Either set the thickness control to cut

thick sections, eg: 20µm, and move the

sledge back and forth repeatedly to trim

the specimen.

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