Cantium Scientific MicroBio MB1 Bedienungsanleitung

MicroBio Operating Manual

MB1 and MB2 Models

Contents

....................................................................................................................Regulatory Compliance!3

....................................................................................EC DECLARATION OF CONFORMITY!3

.........................................................................................................................................RoHS!3

....................................................................................................................WEEE & Recycling!3

................................................................................................................................Introduction!4

.....................................................................................................................Technical Speciﬁcation!4

...........................................................................................................................................Warranty!5

..............................................................................................................Notes on using this manual!5

............................................................................................................................Sampler Operation!5

........................................................................................................................Installing Battery!5

.................................................................................................................Inserting a Plate/Dish!6

..................................................................................................................................................MB1!6

.................................................................................................................Switching On and Off!6

..............................................................................................................Setting Sample Volume!6

.........................................................................................................Starting/Stopping Samples!6

..................................................................................................................................................MB2!7

..............................................................................................................................Take Sample!7

.............................................................................................................................Delayed Start!8

..............................................................................................................................User Presets!8

.................................................................................................................................Unit Status!8

..............................................................................................................................Battery Level!8

....................................................................................................................Total Volume Count!9

..................................................................................................................Total Samples Taken!9

................................................................................................................................Unit Version!9

...........................................................................................................Restore Factory Defaults!9

.......................................................................................................................Determining Results!10

.................................................................................................................Change Plate/Dish Type!11

.........................................................................................................................................Cleaning!11

......................................................................................................................................Calibration!12

....................................................................................................................MicroBio Air Sampling!12

..................................................................................................................................Sampling!12

....................................................................................................................................Hygiene!13

..........................................................................................................Temperature & Humidity!13

...................................................................................................................Selection of Media!13

.............................................................................................................................Troubleshooting!14

...........................................................................................................................Technical Support!14

.....................................................................................................................................Appendix A!15

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Regulatory Compliance

EC DECLARATION OF CONFORMITY

This is to certify that MicroBio MB1 and MB2 products manufactured from January 2006 meet with

the following European Community Directives:

Electromagnetic Compatibility (EMC) Directive 89/336/EEC repealed by 2004/108/EC to the

following standards:

EN 61000-6-3 - Generic Residential & Light Industrial Emissions

EN 61000-6-1 - Generic Residential & Light Industrial Immunity

Signed:

S Plumridge CEng MIET MIEEE FRSA

Managing Director

Cantium Scientiﬁc Limited

Clarendon Gardens

Dartford

Kent

DA2 6EY

United Kingdom

as manufacturer of the appliance.

Document Number 09012006/MB1

Document Number 09012006/MB2

RoHS

We have taken the step of ensuring our products meet these requirements as part of our

commitment to manufacturing goods with the least environmental impact.

WEEE & Recycling

Applicable to all EC States only:

At the end of this product's useful life, please dispose at an appropriate recycling collection point

provided in your country, or return to your distributor who is obliged to take the product for safe

recycling and disposal under the European Directive 2003/108/EC.

Outside of the European Community, consult your local regulations or local distributor.

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Introduction

The MB1 and MB2 MicroBio air samplers are the most economical samplers in the world for

monitoring airborne micro-organisms or bioaerosols.

They have been fully validated by the UK Department of Trade and Industry Validation of Analytical

Methods (VAM) programme to meet the standards required for a reference sampler.

These samplers collect airborne micro-organisms by drawing a stream of air at a constant ﬂow rate

of 100 litres per minute through a series of small holes in a metal head. The air stream then impinges

onto a sterile culture medium in a 55mm contact plate or 90mm Petri dish. After exposure to the air

stream for a ﬁxed period, the contact plate is removed and incubated. The numbers of colonies

which develop are counted, enabling a calculation to be made of the concentration of micro-

organisms in the air (CFU/m3 - colony forming units per cubic metre).

Technical Speciﬁcation

MicroBio MB1

MicroBio MB2

Flow Rate:!

100 l/min

Sample Volume:

25 to 1,500 litres in 14 preset steps

25 to 10,000 litres in 25 litre steps

Sampling Volume

Capacity:

Up to 30,000 litres before recharge*

Up to 60,000 litres before recharge*

Sample Delay range

10 seconds to 3 hours

d50 Particle size

1.7µm (220 x 1mm hole head)

1.35µm (400 x 0.7 mm hole head)

Mean particle velocity

9.62m/s (220 x 1mm hole head)

10.7m/s (400 x 0.7 mm hole head)

Other Features:

Auto switch off

4-digit 7-seg LED display

Sample cancel feature

Padded carry bag supplied

User programmable preset volumes

Delay start sampling

16 character x 2 line backlit LCD display

Tripod mounting bracket

Padded carry bag supplied

Audible indication of operation/status

Sample cancel with volume taken display

Weight (excluding

charger and carry bag):

650g

700g

Dimensions:!

196 x 100 x 110mm (inc. head)

196 x 100 x 40mm (case only)

196 x 100 x 110mm (inc. head)

196 x 100 x 40mm (case only)

Power:!

4 x AA Cells Alkaline or NiMh

6V at 350mA (maximum)

4 x AA Cells Alkaline or NiMh

6V at 250mA (maximum)

Noise Level:!!

< 75dB @ 1m

Environmental Operating

Range:

-10 to 40°C up to 90% RH

Sampling Plate :

55mm contact plate or 90mm Petri dish

Sampling Head:!!

316 grade stainless steel 220 x 1mm diameter

holes

316 grade stainless steel 220 x 1mm diameter

holes or anodised aluminium 400 x 0.7mm diameter

holes

* Based upon random volume samples at varying intervals until low battery warning given.!These tests were undertaken on

units ﬁtted with new and fully charged Ansmann Max-e NiMh cells.!Actual battery life may vary due to volume taken per

sample, interval between samples, age of cells, and other environmental effects, such as humidity and temperature. Results

obtained from other brands of cells may vary.

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Warranty

The manufacturer warrants this product to be free from defects in materials and workmanship for 12

months from the date of purchase.!Your local distributor may offer extended warranties in addition to

this period.

If your product is found to be defective within that period, please contact your local distributor who

will arrange for repair of the instrument, or if necessary a replacement.

This warranty does not cover accidental damage, wear and tear, consequential or incidental loss.

The warranty excludes rechargeable batteries supplied with the sampler. Damage caused by use of

cleaning materials not recommended by the manufacturer will void the warranty.!Any use beyond

the speciﬁcation of the unit will void the warranty.!Any modiﬁcations to the unit without permission or

advice from the manufacturer will void the warranty.

This warranty does not affect your statutory rights.

Notes on using this manual

Operating instructions that follow colour to indicate the following:

RED!:!Control panel button

BLUE!:!Display window message

Sampler Operation

Both MB1 and MB2 models are easy to use hand held instruments.

The MB1 may be hand held or placed on a surface.

The MB2 may be hand held or mounted on a tripod at any height.

Installing Battery

The batteries are held in a small compartment at the back of the MicroBio. The unit is supplied with

4 x AA NiMH high capacity cells. The user can replace these at any time with similar NiMh, NiCd, or

even non-rechargeable Alkaline Batteries. The batteries are removed for re-charging in the "plug in

the wall charger" supplied.

DO NOT ATTEMPT TO RECHARGE ALKALINE BATTERIES using the supplied charger.

When the batteries need re-charging the display will show the words LOW BAT when the unit is ﬁrst

switched on.

We do NOT recommend the use of Zinc-Carbon Batteries. These can leak and when left in a

discharged state in the battery compartment and can cause corrosion, which can damage the

printed circuit board. We also recommend that batteries are removed from the unit if it is to be left

unused for extended periods to avoid corrosion.

Ensure any cells used are designed for high drain applications.

NOTE: Please read instructions for the supplied charger before charging the NiMh cells.

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Inserting a Plate/Dish

For both MB1 and MB2 models, remove the sampling head cover carefully, unless it is being carried

separately in a sterile container. Only hold the edge of the head. Do not touch the front or rear

(inside) surface close to the holes. If necessary the head should be sterilised. Insert a contact plate

inside the springs so that its base sits ﬁrmly on the three support posts; then re-ﬁt the sampling

head.

MB1

The MB1 is an extremely simple to use entry level sampler with basic features to get the job done

with the minimum of fuss.

Switching On and Off

To switch the unit on press any of the four switches.!The display will show the last volume setting

used. The unit will switch itself off automatically if not used for 20 seconds.

Setting Sample Volume

To select a volume of air to be sampled use the +and -!buttons to increase or decrease the amount.

The MB1 has fourteen pre-set volumes of: 25, 50, 100, 150, 200, 300, 400, 500, 600, 750, 800, 1,000,

1,200 and 1,500 litres.

Starting/Stopping Samples

Press the START button to start the sample process.!When running, a red LED at the top end of the

sampler will illuminate. If at any point it is required to stop sampling early, press the CANCEL button.

The MB1 will stop sampling when the required volume of air has been sampled.!The red light will

extinguish at the top of the unit.

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MB2

The MB2 provides a rich set of features to provide a ﬂexible sampling solution with increased battery

life and sampling capacity.

To switch on, press ON/OFF!once. The LCD display will show the product name followed by the ﬁrst

menu option of "Take Sample?"!Press ON/OFF!again to switch off the MB2.!The MB2 does not have

an auto power off facility. The MB2 has a simple menu structure to reach all functions.!The options

are accessed by pressing the MENU button and when the menu item required is displayed on the

screen, START is pressed to select it.!

The main menu options are:

"Take Sample?"

This function performs the sample taking of a given volume of air.

"Delayed Start?"

This function adds a user deﬁnable time delay before starting a sample.!This gives

the ability to leave the MB2 in a sealed environment to start once the operator has left

the room.

"User Presets?"

The user can deﬁne six commonly used volumes of their own to save time in setting up

to take a sample.

"Unit Status?"

This gives information about the MB2 regarding battery, samples taken and version

information.

To take a sample, insert a dish as explained earlier, select a volume of air to sample using “Take

Sample” and proceed to sample the air as described in the following section.

Note:"DO NOT switch off the MB2 when sampling is in progress!

Take Sample

Before setting up to take a sample, remember to have a prepared dish ready in the sampling head.

When Take Sample!is shown on the display,!the user has the option to use one of six preset volumes

or manually set a volume.!The MB2 remembers the last used manual volume. To toggle between

preset or manual mode, use +and -buttons then press START to select the mode.

If in Preset Volume mode, use +and -to select one of the presets. When ready to use that set

volume, simply press START again. Sampling will now start and the fan will run and a red LED will

illuminate at the top of the sampler.

If in Manual Volume mode, use +and -!to set the required volume of air to be sampled.!This may be

between 25 and 10,000 litres of air in increments of 25 litres.! ! Pressing +and -together will set the

volume to 100 litres. When ready to take the sample, press START.

To exit from either manual or preset volume modes before having started a sample, press MENU. At

any stage to cancel sampling, press and hold MENU.

During sampling, the display will show the volume of air taken so far and how long to go, in seconds,

before sampling is complete.

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When sampling is complete, the display will show the message SAMPLING DONE!and the ﬁnal

volume taken. The red LED will ﬂash and a warning tone will be made.!To acknowledge the sample,

press START.

The display will revert back to Take Sample mode.

Delayed Start

The user may wish to undertake sampling remotely.

On some occasions, remote operation of the MB2 is required, such as in sealed clean areas.!To

avoid problems associated with infra red remote controls, i.e. the operator and sampler may not be

in line of sight and windows may not be present, or with wireless types where steel walls may block

transmission, the MB2 employs a time delay start for remote operation.

This allows the operator to set a time between 10 seconds and 3 hours, in variable increments,

before sampling of a user deﬁned volume commences.

When this menu option has been selected, use +and -to change the time delay and press START

when ready. The user can then select the volume required in the same way as in the Take Sample

mode described earlier.

When the sample is started, the delay countdown will begin and the LED will ﬂash slowly, a beep will

sound every second and the display will indicate in how many seconds sampling will start.!Once

sampling has started, MENU can be pressed as normal to cancel.

At any time in setting a delay, press MENU to exit.!Pressing +and -together when setting the delay

will set the factory default delay of 60 seconds.!The MB2 will remember the last used preset delay

for next time, even if the unit has been switched off.

User Presets

Some users ﬁnd that for almost all the sampling they undertake the volume of air sampled is always

the same. At this menu item, the user can set six possible Preset Volumes which are stored even if

the MicroBio is switched off and on.

When this menu item is ﬁrst selected, the user is presented with Preset Volume [1]!and the current

set volume.!This value can be adjusted using the +and -"buttons.!When the desired volume is

reached, press the START button to store the value.

The display then moves onto the second preset volume.!If a particular preset is not to be altered,

skip past by pressing START"until the required preset is reached.!When all preset values have been

set and stored, leave this menu option by pressing MENU.

Unit Status

When Unit Status mode is selected, use the +and - keys to navigate between the various screens.!

Pressing START or MENU will take you back to the main menu list.

Battery Level

This display gives a percentage indication of battery capacity. It is only an approximation and

cannot be used to determine the exact operating hours of volume taking capacity remaining in the

battery.

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Total Volume Count

This displays the total litres of air the MB2 has taken since the last factory reset. This ﬁgure is useful

for the manufacturer’s maintenance and reliability statistics, and may also be used as an indicator for

the user’s regular calibration regime.

Total Samples Taken

This is a count of how many samples have been taken by the MB2 since the last factory reset.

This ﬁgure is useful for the manufacturer’s maintenance and reliability statistics and may also be

used as an indicator for the user’s regular calibration regime.

Unit Version

This displays the hardware and software revision information for the MB2.!It is used by the

manufacturer for repair and maintenance purposes.!The software in the MB2 cannot be upgraded

by the user.

Restore Factory Defaults

When Unit Status mode has been selected, it is possible to perform a factory reset on the MB2 by

holding down the +and -buttons simultaneously.!The display will show on the top line ** UNIT

RESET **!with a row of dots appearing on the bottom line.!When sixteen dots appear, the MB2 will

restart itself.

This has the effect of restoring all factory defaults to the pre-set user volumes and zeroing the

samples taken and total volume counts.

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Determining Results

Once the sample has been taken, the contact plate or Petri dish should be removed immediately

from the MicroBio and the lid replaced on the plate and sealed.

The plate should then be incubated for a period of time and temperature dependant on the

requirements of the media.!Further information can be found in the Microbiology section of this

manual.

Once incubated, the colony growths are then counted, either manually or using an automatic colony

counter. Due to the statistical nature of the sampling method and the chance more than one colony

impinged at one point on the dish, a count correction needs to be performed.!The table in Appendix

A gives the corresponding corrected value based on the sampling head used on the MicroBio.

Alternatively, the following equation can be used to determine a corrected count.

!!!!!

nc=nf

1.075

1.052 - nf

⎛

⎝

⎜

⎞

⎠

⎟

0.483

Where nh

is the number of holes on the sampling head, nfis the number of counted colonies and nc

the corrected count. If the counted colonies, (nf)exceeds the number of sampling head holes holes

(nh), then the mathematics will fail and the results cannot be trusted. If this is the case, then the

sample dish can be considered as overloaded with organisms and the user should consider lower

sampling volumes.

The colony concentration is then the corrected count, above, per volume of air sampled.!The results

are normally expressed in colony forming units per cubic metre.

To convert the corrected count to CFU/m3use the equation:

CFU

m3=1000. nc

Where ncis the corrected number of colonies counted and Vsis the sampled volume in litres.

Spreadsheets to automate the correction process and to collate and present results are available for

free download from:

Excel™: http://www.cantiumscientiﬁc.com/downloads/PHCA.xls

Apple™ Numbers™: http://www.cantiumscientiﬁc.com/downloads/PHCA.numbers

MicroBio Operating Manual [P0001W006]

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