CEM SPRINT Bedienungsanleitung

Corporation

This manual contains proprietary information, which shall not

be reproduced or transferred to other documents or disclosed to others

without prior written permission from CEM Corporation.

CEM is a registered trademark of CEM Corporation.

iTAG is a registered trademark of CEM Corporation.

SPRINT is a registered trademark of CEM Corporation.

SPRINTPAK is a registered trademark of CEM Corporation.

Patents Pending

CEM Corporation

POB 200

3100 Smith Farm Road

Matthews, North Carolina 28106-0200

(704) 821-7015 (phone)

[email protected] (email)

www.cem.com (web site)

www.cemservice.us (service)

Manufactured in the

United States of America

601255

Rev. 6

7/10

Table of Contents

Operating Precautions .......................................................................................................................................................................... iii

Warning and Cautions .......................................................................................................................................................................... iii

Accessories .......................................................................................................................................................................................... iv

Overview ............................................................................................................................................................................................... 1

Sprint Operation ................................................................................................................................................................................... 1

Balance Share................................................................................................................................................................................... 1

Computer Settings............................................................................................................................................................................. 1

System Setup ....................................................................................................................................................................................... 2

External Balance Setup .................................................................................................................................................................... 4

Solution Preparation ......................................................................................................................................................................... 5

Gravimetric Method....................................................................................................................................................................... 5

Volumetric Method......................................................................................................................................................................... 5

Standard Method........................................................................................................................................................................... 5

Fluid Tubing Preparation .................................................................................................................................................................. 6

Homogenizer Installation .................................................................................................................................................................. 6

Wash Station Strainer Installation ..................................................................................................................................................... 7

Printer Ribbon Installation ................................................................................................................................................................ 7

Assembled Sprint System ..................................................................................................................................................................... 8

Sprint Keypad........................................................................................................................................................................................ 9

Navigating with the Sprint Keypad..................................................................................................................................................... 9

Instrument Setup .................................................................................................................................................................................. 10

Administrator ..................................................................................................................................................................................... 10

System .............................................................................................................................................................................................. 10

iTAG® Weight .............................................................................................................................................................................. 10

Preferences ...................................................................................................................................................................................... 11

Data Output....................................................................................................................................................................................... 12

Serial Options.................................................................................................................................................................................... 12

Backup/Restore ................................................................................................................................................................................ 12

Backup .......................................................................................................................................................................................... 12

Restore ......................................................................................................................................................................................... 12

Delete ........................................................................................................................................................................................... 13

Import/Export Data ........................................................................................................................................................................... 13

Import Methods ............................................................................................................................................................................. 13

Export Methods ............................................................................................................................................................................. 13

Export Bundles.............................................................................................................................................................................. 14

Export Results .............................................................................................................................................................................. 14

User Management ............................................................................................................................................................................ 15

New User ...................................................................................................................................................................................... 15

Administrator ........................................................................................................................................................................................ 15

Folder Management .......................................................................................................................................................................... 16

Method Management......................................................................................................................................................................... 16

Sample ID Settings............................................................................................................................................................................ 17

Date/Time.......................................................................................................................................................................................... 17

Balance Settings................................................................................................................................................................................ 17

Balance Share............................................................................................................................................................................... 18

Computer Settings............................................................................................................................................................................. 18

Contrast............................................................................................................................................................................................. 19

SPRINTPAK Setup................................................................................................................................................................................ 20

SPRINTPAK Restandardization ............................................................................................................................................................ 21

Instrument Operation ............................................................................................................................................................................ 22

Load Method ..................................................................................................................................................................................... 22

Edit Method ........................................................................................................................................................................................... 22

Method Parameters........................................................................................................................................................................... 23

Method Name................................................................................................................................................................................ 23

Method Bias .................................................................................................................................................................................. 23

Result Predision ............................................................................................................................................................................ 24

Sample Details .............................................................................................................................................................................. 24

Run Details.................................................................................................................................................................................... 25

Create Method................................................................................................................................................................................... 28

Stop Method...................................................................................................................................................................................... 28

Recovery Clean................................................................................................................................................................................. 29

Statistics ............................................................................................................................................................................................... 29

Maintenance.......................................................................................................................................................................................... 29

System Information................................................................................................................................................................................ 30

Maintenance, Troubleshooting and Service .......................................................................................................................................... 32

Routine Maintenance and Cleaning .................................................................................................................................................. 32

Cleaning Procedures for the Homogenizer Probe............................................................................................................................. 32

Replacement of Peristaltic Pump Tubing (Waste and Optics)........................................................................................................... 33

Preparation for Shipment................................................................................................................................................................... 34

Spill Removal..................................................................................................................................................................................... 35

Optics Cleaning................................................................................................................................................................................. 35

Troubleshooting................................................................................................................................................................................. 36

Ordering Information.............................................................................................................................................................................. 37

Speciﬁcations ........................................................................................................................................................................................ 38

Warranty................................................................................................................................................................................................ 39

Operating Precautions

The SPRINT® System must be grounded. In the event of an electrical short circuit, grounding reduces the risk of electric

shock by providing an escape wire for electric current. This instrument is equipped with a cord having a grounding wire

with a grounding plug. The plug must be plugged into an outlet that is properly installed and grounded. Consult a qualiﬁed

electrician or service technician if the grounding instructions are not completely understood or if doubt exists as to whether

the instrument is properly grounded. If it is necessary to use an extension cord, use only a 3-wire extension cord that has

a 3-blade grounding plug and a 3-slot receptacle that will accept the plug from the instrument. The marked rating of the

extension cord must be equal to or greater than the electrical rating of the instrument.

This instrument utilizes high voltages. Only those trained in repair and maintenance of high voltage systems should

perform instrument service and repair. Ensure that the instrument power cord is removed either from the electrical outlet

or the back of the instrument prior to any maintenance or service and prior to opening the instrument cover.l

Use of the Sprint instrument in any manner not speciﬁed by CEM Corporation could render the instrument opera-

tion unsafe for the operator.

FCC regulations state that any unauthorized changes or modiﬁcations to this equipment, not expressly approved

by the manufacturer could void the user’s authority to operate this equipment.

This device complies with part 15 of the FCC Rules. Opertion is subject to the following two coinditions: (1) This

device may not cause harmful interference, and (2) this device must accept any interference received, including

interference that may cause undesired operation.

Warnings and Cautions

Warnings, cautions and notes are included throughout this manual and should be read thoroughly and strictly followed.

WARNING: A warning is inserted for essential information used to emphasize dangerous or hazardous conditions to the

operation, cleaning and maintenance of the instrument which may result in personal injury.

CAUTION: A caution is inserted for essential information used to emphasize procedures which, if not strictly followed,

may result in damage or destruction to the instrument or improper instrument operation.

NOTE: A note is inserted for emphasis of procedures or conditions that may otherwise be misinterpreted or overlooked

and to clarify possible confusing situations.

WARNING

If damage to the instrument is noted upon receipt, do not attempt to operate the instrument.

This instrument utilizes high voltages. Only technicians trained in repair and maintenance of high voltage

systems should perform instrument service and repair.

Disconnect the instrument from the AC power source prior to performing any maintenance or service pro-

cedure requiring removal of the instrument top cover.

CAUTION

Control for the bottle stirrer and external balance is through cables that connect directly to the Sprint sys-

tem. These connectors should be connected or disconnected only with the Sprint power switch in the “off”

position. If the bottle stirrer cable or balance cable is connected with the system on, damage to the I/O

board may occur.

iii

Accessories

Verify that all accessories are included with the Sprint

instrument.

558005 Bottle Stirrer

246075 Bottle Stirrer Cable

176965 Printer Paper (5 Rolls)

558200 Solution 3 Bottle Assembly

558205 Solution 2 Bottle Assembly

558210 iTAG® Solution 1 Bottle Assembly (2)

177500 Wash Station Strainer

BR851035 Wash Station Cap

177045 Pitcher

BR119690 External Balance Cable

177165 Homogenizer Upper Bearing

177170 Homogenizer Teﬂon Bearing

1771601 Homogenizer Tool (2)

177325 Stirring Bar (2)

277200 Jump Drive

558150 Water Line Assembly

177260 Silicone Tubing

177150 Optics Pump Tubing

177151 Waste Pump Tubing

168575 Funnel

177310 Spatula

177315 Spatula

BR188270 10 AMP Fuse (2)

BR188280 5 AMP Fuse (2)

177450 Power Adaptor

BR875000 Rectangular Container, Filter, and Lid (2)

601255 Sprint Operation Manual

912490 Anti-Static Ionizer (120V) or

912495 Anti-Static Ionizer (240V)

572015 Standard Homogenizer or

572025 Meat Homogenizer (Beef/Special Meats)

SPRINTPAK® (Sold Separately)

912550 SPRINTPAK (4 Pack)

Optional accessories:

912455 External Pump Assembly Kit

920926 External Balance

912485 Grain Sample Preparation Kit

Cleaning Kits:

912420 Homogenizer Cleaning Kit

912445 Optics System Cleaning Kit

912425 Filter Kit

iTAG Solution Mixing Kits:

912510 Gravimetric Kit

912520 Volumetric Kit

Maintenance Kits:

720700 6-Month Maintenance Kit

720705 12-Month Maintenance Kit

912440 Tubing Kit

912430 Homogenizer Bearing Kit

Instrument components considered consumables:

Tubing

Fuses

Bottle Assemblies

Stirring Bars

Pitcher

Funnel

Printer Paper

Rectangular Containers

Spatulas

Overview

The SPRINT® Protein Analyzer is automated and determines

the amount of protein in a sample in approximately 2 minutes.

This rapid technique is easy to use and utilizes a proprietary

iTAG solution, which attaches to protein molecules, to mea-

sure the concentration of protein in a sample.

Unlike other conventional methods, Sprint does not require

the use of harmful chemicals, such as sulfuric acid, sodium

hydroxide, potassium sulfate, mercury oxide or copper sul-

fate. Following the protein test, there is no hazardous waste

for disposal. The sample and non-toxic iTAG® Solution 1 are

continued within the sample cup for easy disposal.

The iTAG® solution is completely non-toxic and is not con-

sidered a hazardous material. No special waste disposal is

required, and there are no known health hazards associated

with the solution. The cleaning solution (Solution 3) is used

after analysis to prepare the system for subsequent tests.

This solution is also non-toxic, not hazardous and requires no

special handling for disposal.

The following is a description of each component and subsys-

tem in the Sprint system as it is used in a typical analysis.

The system uses three input solutions: iTAG® Solution 1, So-

lution 2 to be mixed with deionized water, and Solution 3 to be

mixed with water for cleaning. These solutions are contained

in three bottles that are positioned in a bottle stirrer beside the

system. The iTAG® Solution 1 bottle is positioned in the back

position; Solution 2 is in the middle position, and Solution 3 is

in the front position. The positioning of these bottles is critical.

The bottle stirrer contains an electromagnetic stirrer for posi-

tion 1 to stir the iTAG® solution.The position for each bottle

connector is labeled on the rear of the instrument and on the

side of the bottle stirrer.

CAUTION

Control for the bottle stirrer is through a cable

that connects directly to the Sprint system.

This connector should be connected or

disconnected only with the Sprint power

switch in the “off” position. If the bottle stir-

rer cable is connected with the system on,

damage to the I/O board can occur.

A water connection is required for system operation. The

minimum ﬂow rate is 30 mL/sec at 1.5 Bar (22 psi). A pres-

sure regulator and water ﬁlter is mounted to the back of the

instrument. A water line is provided with a ¼” Swagelok ﬁtting

on a ¼” (outer diameter) tube. The water supply can either

be plumbed to a pressurized water supply or connected to the

external water option available from CEM.

The external water option consists of a carboy, pump and

connections for control and power. External waste is routed

to the back of the Sprint system. A 3/8” ﬂexible tube is provide

to connect to a waste drain or waste carboy. All Sprint waste

solutions are non-toxic and non-hazardous.

The anti-static ionizer emits a stream of alternating positive

and negative ions which effectively eliminate static charge on

the sample cups prior to placing them in the external balance.

The result is more accurate sample weights, therefore, more

accurage sample results.

SPRINT® Operation

1. A sample cup is waved in front of the anti-static ionizer to

eliminate static charge, then placed on the external bal-

ance. “TARE” is pressed.

2. A sample is placed in the cup on the balance and

weighed.

3. The cup containing the sample is removed from the bal-

ance and placed in the turntable.

4. A ﬁlter is placed in the turntable.

5. Sprint dispenses a known amount of iTAG® solution in

the sample cup.

6. The sample and iTAG® solution are homogenized, re-

leasing all proteins to bind with the iTAG® and forming an

insoluble complex that drops to the bottom of the cup.

7. The ﬁlter is lifted from the ﬁlter holder.

8. The homogenizer is cleaned with warmed jets of mild

detergent.

9. A portion of the remaining iTAG® solution is drawn

through the ﬁlter into the detector to determine the

amount of iTAG® that was bound to the protein.

10. Sprint calculates the % protein present in the sample.

11. The ﬁlter is released by the detector inlet and placed in

the sample cup.

12. The cup containing the sample, ﬁlter and iTAG® solution

is removed, capped and disposed of.

Balance Sharing

The Sprint can be conﬁgured to share one external balance

with two instruments. One instrument is designated as the

“Main” instrument, and the second instrument is designated

as the “Remote” instrument. This permits the weighing of a

second sample for testing in the “Remote” instrument while

the ﬁrst sample is being tested in the “Main” instrument. Setup

for balance share is described in the “Instrument Setup” sec-

tion of this manual

Computer Settings

The Sprint has an assigned IP address which is used to iden-

tify it when it is connected to a network or when it is sharing

a balance with another Sprint. If the Sprint is connected to a

network, the network administrator will assign an IP address

to be entered in “Computer Settings.” This requires comple-

tion prior to system setup for sharing a balance.

1. Carefully open the shipping

carton. Lift the foam protec-

tor from the carton, expos-

ing the plastic-covered

Sprint instrument.

2. Lift the protective plastic,

exposing the instrument.

3. Using the provided lifters,

carefully lift the instrument

from the carton and place it

on a sturdy work bench with

the back of the instrument

positioned so that all con-

nections can be accessed.

CAUTION

Do not attempt to lift

the instrument by the

top cover.

4. Using a small ﬂat blade

screwdriver, open the cover

of the power entry module,

exposing the fuse holder.

5. Using a small ﬂat blade

screwdriver, remove the

fuse holder from the power

entry module.

6. Based on electrical require-

ments, install the two (2)

applicable fuses in the fuse

holder.

10 AMP for 120V (BR188270)

5 AMP for 240V (BR188280)

System Setup

7. Install the fuse holder into

the power entry module and

close the cover.

8. Unscrew the cap from the

connector of the water/ﬁlter

regulator. Note: Retain the

cap for reuse if the instru-

ment requires relocation

or return to CEM for repair.

Refer to the “Maintenance,

Troubleshooting and Ser-

vice” section of this manual.

9. Locate the 10’ length of tub-

ing shipped with the instru-

ment. Connect one end of

the tubing to the connector

on the water ﬁlter/regula-

tor assembly, and the other

end to a laboratory water

source.

10. Turn on the water source to

supply water to the instru-

ment.

11. The pressure gauge on

the ﬁlter/regulator assem-

bly is factory set to 10 psi.

If necessary to reset the

pressure, rotate the pres-

sure adjusting nut clockwise

(right facing back of instru-

ment until the gauge on the

ﬁlter/regulator assembly

indicates 10-15 psi. Note:

If any water leakage is de-

tected, discontinue system

installation and contact

CEM Service.

12. Remove the black cap

from the connector labeled

“Waste.” Note: Retain the

cap for reuse if the instru-

ment requires relocation

or return to CEM for repair.

Refer to the “Maintenance,

Troubleshooting, and Ser-

vice” section of this manual.

13. Using the length of 1/4”

silicon waste tubing, con-

nect one end of the tubing

to the waste connector on

the back of the instrument.

Position the other end of

the waste tubing in a waste

bottle, sink or other suitable

waste container.

14. Secure the waste tubing

to prevent spillage due to

pressure.

15. Carefully open the shipping

carton containing the bottle

stirrer assembly. Remove

the bottle stirrer from the

foam packaging.

16. Position the bottle stirrer on

the work bench on the right

side of the instrument (fac-

ing the front of the instru-

ment).

17. Ensure that the instrument

is not turned on. Connect

the cable supplied with the

instrument accessories to

the back of the bottle stirrer

by carefully aligning the pins

of the connectors. Once

the connector is properly

seated, tighten the screws

of the connector.

18. Connect the other end of

the cable to the 16-pin

connector on the back of

the instrument by aligning

the pins of the connec-

tors. Once the connector is

properly seated, tighten the

screws of the connector.

CAUTION

If the bottle stirrer cable

is connected with the

system on, damage to

theI/Oboardmayoccur.

19. Locate the three tubes

extending from the back of

the bottle stirrer. Connect

the tubing labeled “iTAG

1” to the connector on the

back of the Sprint instru-

ment labeled “iTAG Solution

1” by pushing the connector

on the tubing into the con-

nector of the instrument and

rotating it clockwise.

20. Connect the tubing labeled

“2” to the connector on the

back of the instrument la-

beled “Solution 2” by push-

ing the connector on the

tubing into the connector of

the instrument and rotating

it clockwise.

21. Connect the tubing labeled

“3” to the connector on the

back of the instrument la-

beled ”Solution 3” by push-

ing the connector on the

tubing into the connector of

the instrument and rotating

it clockwise.

23. Using an index ﬁnger

positioned as shown in the

illustration, carefully lift the

instrument cover.

22. Using a Phillips screwdriver,

remove the screw securing

the instrument cover. The

screw is located on the un-

derside of the cover to the

right of the barcode reader.

24. Either by hand or with a

ﬂat blade screwdriver as

required, rotate the spring

loaded screw securing the

Z axis counterclockwise

(loosen) until the tension of

the spring is released. Note:

Do not completely remove

the screw.

25. Carefully lower and close

the instrument top cover.

26. Remove the tape securing

the instrument turntable

door.

Inhaltsverzeichnis

Andere CEM Analytisches Instrument Handbücher

CEM

CEM WT-70S Bedienungsanleitung

CEM

CEM LDM-40 Bedienungsanleitung

Beliebte Analytisches Instrument Handbücher anderer Marken

Cocraft

Cocraft NLT04 Bedienungsanleitung

M&C

M&C GENTWO PMA1000 Bedienungsanleitung

MICRO-EPSILON

MICRO-EPSILON optoncdt 1700 Bedienungsanleitung

CONDTROL

CONDTROL XP4 PRO Bedienungsanleitung

Extech Instruments

Extech Instruments BR200 Bedienungsanleitung

Dewetron

Dewetron DEWE-30-16 Produkthandbuch

EXTOL PREMIUM

EXTOL PREMIUM 8820043 Montage- und Bedienungsanleitung

RIDGID

RIDGID SeeSnake Compact Bedienungsanleitung

Teledyne

Teledyne 3000PA Bedienungsanleitung

YDLIDAR

YDLIDAR TIA Bedienungsanleitung

Olympus

Olympus IPLEX FX Series Bedienungsanleitung

Bosch

Bosch GLM 50 C Professional Bedienungsanleitung

Reflecta

Reflecta DigiEndoscope Bedienungsanleitung

Chinavision

Chinavision CVACZ-G696 Montageanleitung

Fuji Electric

Fuji Electric ZKMA Bedienungsanleitung

Teledyne

Teledyne 3300TA Bedienungsanleitung

VOLTCRAFT

VOLTCRAFT IC-100HD Bedienungsanleitung

RIDGID

RIDGID micro CA-25 Bedienungsanleitung