Contherm MITRE 4150 Bedienungsanleitung

Scientific Ltd

SERVICE MANUAL

MITRE 4000 SERIES

Sterilising - Standard/Tropicool/Cooled Models

CO2CELL CULTURE INCUBATORS

CONTHERM SCIENTIFIC LIMITED

P O BOX 30-605 LOWER HUTT 5040

TEL: (0064-4) 568 8034

FAX: (0064-4) 568 8095

EMAIL: contherm@xtra.co.nz

WARRANTY STATEMENT

*******

CONTHERM Scientific Company will guarantee CONTHERM equipment for

aperiod of twelve months from the date of installation against faulty

workmanship and fabricated materials. This guarantee covers the

replacement of component parts found to be defective and authorised

labour charges during this period.

Should a malfunction occur or condition develop beyond reasonable

acceptance the company will accept responsibility for returning the unit to

its factory specification at no cost to the Purchaser providing that the

operating instructions have been observed and the defect is due solely to

faulty design, material and workmanship. That the defective part be

returned, freight paid to the nearest sales service office. Units outside the

warranty period will be accepted and repairs will be covered under an

extension of the above for 90 days.

In remote installations where it is not possible for the company's or

agents' engineers to attend, authority may be given to allow the Purchaser

to arrange such service.

The Purchaser is required to remit the purchase price of the unit to the

supplier within the terms of that supplier's condition of sale. CONTHERM

Scientific Company will accept no liability or shall its agents for

consequent damage of any kind due to a malfunction or component

failure.

*******

MANUALP/4000_SERVICE_0712 July2012 4

STATEMENT of CONFORMITY

This CONTHERM cabinet conforms to the following standards:

•Electrical Safety: Designed to NZS6200 / AS3100:1994

•EMC: Complies with AS/NZS 2064: 1997

MANUALP/4000_SERVICE_0712 July2012 5

CONTENTS

SECTION 1 Definition of Terms

SECTION 2 Introduction and Specifications

SECTION 3 Installation

SECTION 4 Operating Instructions

SECTION 5 Calibration

SECTION 6 Theory of Operation

SECTION 7 Customer Troubleshooting and Maintenance

SECTION 8 Spare Parts Lists

SECTION 9 Fault / Alarm Codes

SECTION 10 Servicing / Replacing Parts

IMPORTANT All electrical servicing must be carried out by

suitably qualified personnel only.

MANUALP/4000_SERVICE_0712 July2012 6

SECTION 1 DEFINITION OF TERMS

For the purpose of our standard specifications the following definitions shall

apply:

a) WORKING SPACE

That portion of the internal space which is above the lowest shelf and

not less than 5cm from any wall (including roof).

b) CHAMBER TEMPERATURE

That temperature at the centre of the working space.

c) SPATIAL VARIATION

The difference between the midrange of all measured temperatures

obtained at one site and that at another site for those sites which give the

greatest difference.

d) TEMPORAL VARIATION

The maximum value of the temperature range obtained for the standard

site with the greatest range throughout the test interval.

e) TEMPERATURE OVERSHOOT

Any excess of actual over desired chamber temperature during a

heating up period.

f) TEMPERATURE REPRODUCIBILITY

Temperature regained without alteration to controls.

g) TEST INTERVAL

Interval of time to which the steady state characteristics apply (Max 1

hour).

NB: All the above apply with an empty chamber.

For definitions and test methods refer: AS2853 : 1986 (and Appendices)

MANUALP/4000_SERVICE_0712 July2012 7

SECTION 2 INTRODUCTION AND SPECIFICATIONS

The CONTHERM CAT4000 Mitre Series of Co2 Culture Incubators has been

expressly designed to give the widest choice of operating conditions consistent

with high reliability and low cost, the addition of microprocessor control gives

additional versatility and resolution while improving the degree of temperature

control. These Incubators are fitted with a UVC (254nm) SERILISING UV lamp.

All specifications are quoted for an enclosure temperature of +37oCwith an

ambient Temperature of +20oCfor Standard and Tropicool cabinets and for an

enclosure temperature of +20oCfor cooled cabinets.

•Construction - Combination of High quality stainless steel and zinc plated

mild steel; epoxy coated exterior, full fibreglass insulation, High quality

stainless steel interior with full access door and magnetic door catch

•Safety - Fitted with an independent factory set Hi-Limit and user adjustable

Hi-Limit completely separate from normal controls.

•Convection - All units come with mechanical convection fan systems.

•Electrical - Designed to NZS6200 / AS3100:1994 240V AC M.E.N

•EMC - Complies with AS/NZS 2064: 1997

•All performances quoted at 20oC, ZP22 Microprocessor control System.

PERFORMANCE:

a)Temperature:

Nominal Range: Standard (Ambient +5oC) to +50oC

Tropicool (Ambient -5oC) to +50oC

*Cooled +15oCto +50oC

Temporal Variation Standard +_ 0.2oC

(Tropicool) +_ 0.3oC

(Cooled) +_ 0.4oC

Spatial Variation Standard +_ 0.4oC

(Tropicool) +_ 0.4oC

(Cooled) +_ 1.5oC

Initial Overshoot +2.0oC

Reproducibility +_0.5oC

Dial resolution 0.1oC

Operating Ambient 10oC - 35oC

Mains Voltage Range 230-250 AC 50Hz

*NB: Can only be operated on 60Hz when cooled units fitted with a special

refrigeration compressor.

MANUALP/4000_SERVICE_0712 July2012 8

NB: The lowest temperature performance is only achievable in a maximum

ambient of +20oC

NB: If fitted with a HEPA filter, the spatial specification is downgraded by a

further ±0.4oC

b) Relative Humidity -

With the standard tray of water inside a standard cabinet the humidity will

typically rise to 80% RH or more at a temperature of 37.0oC. The

maximum achievable humidity will be less in a Tropicool or Cooled

cabinet.

NB: On COOLED units the supplied stainless steel water tray should be placed

on the cabinet floor and pushed so that the sloping side firmly touches the rear

wall.

c) Carbon Dioxide:

Range: 0.5 - 20 % CO2

Fluctuation (@35Kpa / 5PSI) ±0.2% CO2

Accuracy: (@37oC, 85% RH) ±0.5% CO2

CAUTION: This chamber has been fitted with a SPECIAL UVC Sterilising lamp

located behind the stainless steel rear duct. Note that this ULTRA-VIOLET

lamp is HARMFUL to the eyes and MUST NOT be viewed directly or indirectly

for any period.

The lamp is automatically turned OFF when the outer door is opened and turns

on for 5 minutes when the outer door is reclosed.

If for ANY reason the glow from the lamp can be seen when the outer door is

opened, the cabinet must be turned OFF and the cause investigated by a

qualified service engineer.

SPECIAL NOTE for COOLED incubators

COOLED chambers may be fitted with a ‘FRIDGE’ switch located on the RHS

monitor panel. If fitted, this switch allows the user to turn the refrigeration

system OFF when operating at higher temperatures and low ambient condition

(for example at 37oCin an air-conditioned room).

If the ‘FRIDGE’ switch is turned ON or OFF when the chamber has stabilised at

temperature, the control temperature may be disturbed for a short period while

the chamber re-stabilises at the new load setting.

When operating at 37oCin an air-conditioned room, it is recommended to

set the ‘FRIDGE’ switch to OFF.

MANUALP/4000_SERVICE_0712 July2012 9

SECTION 3 INSTALLATION

The CAT 4000 Mitre Series Incubators are designed to be installed into a

suitable well ventilated room with a preferred minimum clear space of at least

150mm on all sides to allow access for servicing. Mount the enclosure in its

final place ensuring that adequate space is allowed for the door to open. The

enclosure may be placed on the floor or a bench.

NB: On Tropicool and Cooled units ensure a space of at least 150mm is

allowed at the rear of the cabinet for air circulation – On Tropicool units ensure

that the unit can not be pushed too close to a rear wall. Locate the two stainless

perforated “butterfly wings” at the rear sides of the Tropicool unit and gently

bend to an angle of 90o.

The chamber requires a 240Volt 50HZ 10Amp Single phase EARTHED

electricity supply.

Total electrical load is upto 0.75Kw (4400C).

Maximum shelf loading is 30Kg/shelf, maximum total for cabinet is 100Kg.

The chamber should be thoroughly inspected for any signs of mechanical

damage that may have occurred in transit before any attempt is made to apply

power to the unit.

1) Connect a bottle of DRY FOOD GRADE CO2fitted with a two stage

Pressure Regulator set at 35kpa (5psi) to the Hosetail No 1 at the rear

of the enclosure.

2) If the enclosure is fitted with the automatic CO2CHANGEOVER option fit

the second gas bottle to Hosetail No 2. This bottle will automatically be

selected when bottle No 1 is empty.

3) Install the shelf guides in the desired positions. Slide the shelves into

guides.

4) On cooled / Tropicool units a large stainless steel or plastic tray is used to

both provide and collect water from the refrigeration system evaporator.

Ensure this tray is pushed firmly against the rear wall of the cabinet. If

elevated humidity conditions are desired partially fill the tray with distilled

or deionised water.

NOTE: The hosetail is a nominal ¼” brass barb. Typically use 6.0mm ID PVC

beverage tubing or similar to connect to the gas bottle regulator output. The

tubing should be secured to the hosetail using S/S hose clips.

MANUALP/4000_SERVICE_0712 July2012 10

WARNING: To prevent FIRE or SHOCK hazard, DO NOT expose this

product to rain or any type of moisture.

FOR YOUR SAFETY

To ensure safe operation the three-pin plug supplied must be inserted

ONLY into a standard three-pin power outlet which is effectively earthed

through the normal building wiring.

Extension cords are NOT recommended.

The fact that the equipment operates satisfactorily does NOT imply that

the power outlet is earthed and that the installation is completely safe. For

your safety, if in any doubt about the effective earthing of the power outlet,

consult a qualified electrician.

This appliance should be regularly tested (or after any service work on

the appliance) according to the procedures prescribed in AS/NZS 3760.

The basic safety checks and tests on electrical appliances required by

AS/NZS 3760 are:

a) A visual check to ensure that there is no mechanical damage, that

controls etc. are in good working order and that no parts are

missing.

b) An earth continuity test. (Maximum allowed resistance is 1 )

An insulation resistance test. (Minimum insulation resistance is

1 M)

WARNING: This cabinet is fitted with a UV Sterilising Lamp

UVC refers to shorter UV wavelengths, usually 200 to 280 nM. Wavelengths

in the UVC range, especially from the low 200's to about 275 nM, are

especially damaging to exposed cells. Such shortwave UV is often used for

germ killing purposes. The typical wavelength for ‘sterilising’ purposes is

253nM.

CAUTION: This chamber has been fitted with a SPECIAL UVC Sterilising lamp

located behind the stainless steel rear duct. Note that this ULTRA-VIOLET

lamp is HARMFUL to the eyes and MUST NOT be viewed directly or indirectly

for any period.

The UV lamp is automatically turned OFF when the outer door is opened and

turns on for 5 minutes when the door is reclosed.

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