Table of Contents
Introduction ................................................................................................................................6
1. General Information ...............................................................................................................7
1.1 Intended Use and modifications of the instrument...............................................................7
1.2 Delivery Set..........................................................................................................................8
1.3 Technical specification.........................................................................................................8
1.4 Operating Conditions..........................................................................................................10
1.5. PC Requirements...............................................................................................................10
2. Instrument description and principle of operation................................................................10
2.2 Fast thermal control system................................................................................................11
2.3 Optical system ....................................................................................................................12
2.4 Control and display system ................................................................................................12
2.5. Marking. Symbols used for marking.................................................................................13
3. Setting up the instrument......................................................................................................15
3.1 Instrument unpack ..............................................................................................................15
Instrument Carrying...........................................................................................................15
3.2 Instrument installation........................................................................................................15
3.3 Software installation...........................................................................................................16
3.3.1. Instrument Driver Installation..................................................................................16
3.3.2. Installation of RealTime_PCR software..................................................................22
4. Operating procedure .............................................................................................................25
4.1. Instrument Switching-on and RealTime_PCR Launch.....................................................25
4.2. Preparing the Instrument for PCR.....................................................................................27
4.2.1. Checkup of geometrical settings..............................................................................27
4.2.2. Optical measurement exposure setting ....................................................................27
4.2.3. Checking the Cleanness of Thermal Plate Wells.....................................................28
4.2.4. Tubes Height Measurement.....................................................................................28
4.3 Creation of plate protocol and amplification program .......................................................29
4.3.1. Filling in of plate protocol .......................................................................................29
4.3.2. Creation/editing of amplification program ..............................................................30
4.4. Placement of tubes with samples.......................................................................................31
4.5 Startup of the amplification program..................................................................................33
4.6 Amplification program running..........................................................................................34
5. Maintenance..........................................................................................................................35
5.1 General guidelines..............................................................................................................35
5.2 Maintenance procedure ......................................................................................................36
5.3 Disinfection requirements of the instrument prior to maintenance and repair procedures.36
5.4 Troubleshooting..................................................................................................................37
6. Storage and shipment ...........................................................................................................38
7. Recycling..............................................................................................................................38
8. EMS declaration...................................................................................................................38
9. Warranties.............................................................................................................................39
11. Acceptance certificate.........................................................................................................41
12. Cards of warranty repair.....................................................................................................43
13. List of claims and instrument disinfection procedure ........................................................45
