Germfree BBF 2 Bedienungsanleitung
Germfree - Class II, Type A2 Biosafety Cabinet User Manual - v2.1 - 09SEP2021 - RMH
Class II, Type A2 Biosafety Cabinet
User Manual
Class II, Type A2 Biosafety Cabinet
User Manual
2
Contents
Introduction ........................................................... 3
Installation ............................................................ 4
Electrical Services ...................................................... 5
Control of Airflow ...................................................... 6
Airflow Diagram........................................................ 7
Filters ................................................................. 8
Certification and Repair........................................... 8
Contoured Viewing Panel ............................................... 9
Cleaning the Viewing Panel ....................................... 9
Stainless Steel Work Deck............................................... 10
Motor Blower .......................................................... 11
Lights ................................................................. 12
Performance Testing.................................................... 13
Use of the Biosafety Cabinet ............................................ 14
Advance Planning: ............................................... 14
Good Technique:................................................. 14
Biological Safety Cabinet Operation “Don’ts”....................... 15
Troubleshooting ....................................................... 16
Dimensions............................................................ 18
Parts List .............................................................. 19
Quality Statement ..................................................... 23
Our Company ......................................................... 23
Germfree’s Mission .................................................... 23
Appendix A - Line Drawings
Appendix B - Wiring Diagrams
Appendix C - Sensocon User Manual
Class II, Type A2 Biosafety Cabinet
User Manual
3
Introduction
The Class II, Type A2 Biosafety Cabinet is a minimal turbulence, unidirectional, vertical down flow re-circulating
laminar flow cabinet, providing a safe work area for handling particles and aerosols down to the sub-micron size
range. This chamber eliminates cross-contamination and accidental release of pathogenic microorganisms or
carcinogenic aerosols to the outside atmosphere. The unit can be used wherever sterile air is required. Other
applications include handling materials such as pollen, powders, allergens, dust, pathogen-free animals and
plants—actually in any situation where both clean air and containment are required. All of the air that bathes the
work area and all the air that is exhausted is passed through High Efficiency Particulate Air (HEPA) filters that are
parallel to each other and to the work surface. These filters remove particulates 0.3 micron in size with 99.99%
efficiency, and they even more efficient for both larger and smaller particulates.
Perhaps the most valuable parameter of the Class II, Type A2 Biosafety Cabinet is containment without the
necessity of working through gloves. This is accomplished by discharging approximately 30% of the total filtered
air each cycle through the exhaust HEPA filter in the top of the unit.
An equal amount of make-up air is pulled into the chamber through the boom of the window under negative
pressure from the blower and positive pressure from the air curtain. The viewing panel has a unique, recessed,
contoured shape, which creates an aerodynamic flow paern. It is this combination that produces the protective
barrier for the operator by containing hazardous materials within the chamber.
Biomedical engineering and technology have introduced safeguards into the design of laboratory equipment,
but these safeguards do not prevent human error. Danger to personnel from carelessness or improperly used
equipment cannot be overemphasized. There is no substitute for safe, proper technique.
Contact Germfree Customer Service
+1 386.265.4300
Class II, Type A2 Biosafety Cabinet
User Manual
4
Installation
The Class II, Type A2 Biosafety Cabinet is ordinarily transported and moved in the upright position. The 3’, 4’ and
6’ units will fit through any opening 32” wide and 58” high. If narrower doors are encountered, the light housing
can be taken off by removing four screws. Alternatively, the viewing panel with the aached light housing can
be completely removed by sliding the split hinges to the right. Removing this panel will reduce the depth of the
biosafety cabinet to 28¾”.
2’ units will fit through any standard doorway.
Class II, Type A2 Biosafety Cabinet
User Manual
5
Electrical Services
The Class II, Type A2 Biosafety Cabinet operates on an 115v, 1-phase, 60 Hertz, 10 Amp electrical system. During
shipment, the power cord is coiled within the control panel. The plug of the power cord is a NEMA 5-15P
3-pronged hospital grade and should be connected to the appropriate 3-prong 115v receptacle. For convenience
of operations and service, all circuitry is located within the control panel.
Standard services include the Motor ON/OFF switch, light switch, a Sensocon or Magnehelic (see front cover
image) gauge at the front of the unit and a speed control located in the control panel behind a plastic cap.
Optional duplex electric outlets may be located in the rear of the work area and optional data ports and/or
stopcocks may be placed at either side at the front of the unit, according to your specifications.
Class II, Type A2 Biosafety Cabinet
User Manual
6
Control of Airflow
To be sure your unit is functioning properly, the air velocity should be measured directly by a qualified third party
certification professional. Readings of air velocity, measured in feet per minute, can be made with a velometer
or thermoanemometer to determine if the unit is within specification and when the filters should be changed.
This certification should be performed at least annually, and many regulatory bodies recommend semi-annually.
The unit must also be recertified any time that it is relocated. The following chart represents the acceptable
range of air velocities that are measured in your unit.
Unit Inflow (fpm) Downflow (fpm) Volume (cfm) Exhaust Flow (fpm)
BBF 2 87-102 65-80 114-134 154-181
BBF 3 102-112 67-82 147-161 123-134
BBF 4 102-112 67-82 215-236 130-142
BBF 6 102-112 70-85 353-388 106-117
Eventually, the HEPA filters will be loaded with particulates to a point where the blower can no longer overcome
the resistance to provide the proper air velocities. At this point new HEPA filters must be installed. Replacement
HEPA filters can be ordered ahead of time so that they are available in advance of changing. Because viable
particles may be trapped and survive for a period of time in the filters, the Class II, Type A2 Biosafety Cabinet
must be decontaminated, if any biological agents have been manipulated within the cabinet, before any HEPA
filters are changed.
A low flow alarm will indicate when the inflow has fallen below acceptable levels. This can indicate that
maintenance needs to be performed on the cabinet.
Class II, Type A2 Biosafety Cabinet
User Manual
7
Airflow Diagram
Class II, Type A2 Biosafety Cabinet
User Manual
8
Filters
All biosafety cabinets are provided with both a supply HEPA filter over the work area and an exhaust HEPA filter
in the top of the unit. All filters were subjected to a particle challenge leak test at the time of manufacturing and
again immediately before shipment and are certified to be 99.99% effective on a .3 Micron particle, the hardest
to capture. Loading time for the filters will depend primarily on the number of particles in the ambient air and
ordinarily, to a lesser extent, on particles generated within the chamber. Under most conditions the life of the
filters will be three to five years; rarely do they become obstructed in three years or less. The biosafety cabinet
may be le on continuously or turned off and on as needed with a ten-minute start up and cool down period
prior to and aer use.
An independent certification agency or a qualified technician specializing in the repair and testing of this type
of equipment must perform all maintenance to the Class II, Type A2 Biosafety Cabinet, especially the filters. The
Germfree service department can assist with or perform most repairs.
Certification and Repair
All models of Class II, Type A2 Biosafety Cabinets have a large supply and a smaller, exhaust HEPA filter. These
are sized to provide the entire work area with ultra clean air and to filter particulate maer. Approximately 30%
of the circulated air is exhausted during each cycle. HEPA filters are placed and sealed within the cabinet to
prevent any bypass of air. Filters are certified to be 99.99 % effective against 0. 3 micron sized particles and free
of any leaks discernible by this method. In the majority of instances, filters received from the manufacturer are
free of leaks. Those that are not are repaired and retested. Testing also includes making sure that the junctions
of the filter medium and framework must be completely free of leaks. This testing also includes checking the
seal around the filter itself. Should damage occur to the filters or if aer replacement, defects are found in new
filters, these defects can be repaired. This requires sealing with RTV (Room Temperature Vulcanizing) silicone.
Commercial organizations with proper equipment and personnel are available in most areas of the world
for testing and servicing. If, you need us to refer you to one of these agencies please contact Germfree (cs@
germfree.com).
Class II, Type A2 Biosafety Cabinet
User Manual
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Contoured Viewing Panel
The shape of the viewing panel was designed to put the operator above the work with a glare free view of all
parts of the work area.
The viewing panels are hinged and can be raised to more than a 90-degree angle to provide easy access for
larger items (shocks or chains are provided to support the window in the open position). To remove the panel, it
is recommended that two people work together, one at each end. Before removal, unplug the light housing cord
from the electrical outlet located at top right corner and remove the small snap ring from the right-most hinge.
If the unit has shocks, they must be detached prior to removal. Next, raise the viewing panel until it is parallel
to the floor and slide the panel toward the right side, completely separating the split hinges. Be sure to replace
snap ring aer use.
Cleaning the Viewing Panel
• The standard viewing panel is acrylic and can be cleaned with a so cloth.
• Do not use abrasive cleansers or organic solvents on this viewing panel.
• The acrylic should not be cleaned with anything stronger than 50% Ethyl or 70% isopropyl alcohol solution.
• Do not use glass cleaner with ammonia.
Class II, Type A2 Biosafety Cabinet
User Manual
10
Stainless Steel Work Deck
With the viewing panel removed (or raised and supported by the shocks / liing chains), the work platform and
its support can be easily separated and removed. If the Class II, Type A2 Biosafety Cabinet has been used with
potential pathogens, the cabinet should first be decontaminated before disassembling.
During routine cleaning of the work platform, its perforated support and the rear and side walls can be
decontaminated by using a sponge or squeeze bole containing a biocide specific to the organisms being
worked with. Spilled material within the chamber should be made safe by the addition of an appropriate
germicide, followed by the appropriate cleaning procedures. For initial cleaning and daily maintenance, the
interior of the chamber should be completely wiped with 70% alcohol, using a so cloth. Aer cleaning, surfaces
should be wiped down with sterile water to prevent chemicals from damaging the surfaces. The outside of the
all stainless steel units may be similarly maintained.
Dieses Handbuch passt für folgende Modelle
7
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