GL-Tip™ SDB Instruction Manual
Thank you for purchasing GL-Tip™ SDB. GL-Tip SDB is a sample preparation tip column packed with styrene di vinyl
benzene polymer designed for desalting and enriching peptide samples, particularly hydrophobic phosphopeptides.
GL-Tip™ SDB is often used in combination with GL-Tip™ GC (available separately, P/N 7820-11201) to ensure that
peptides with the widest range of polarity are captured in high yields.
1. Product Description
○ Check for damage or missing parts.
2. Product Handling
○ Handle GL-Tips delicately; avoid dropping or tapping the tips, which may harm the column bed.
○ Do not autoclave GL-Tips
○ GL-Tips are designed for use with a centrifuge. Attempting to use GL-Tips with syringes, pipettes, or
any air-forced solvent flow methods may result in reduced or irreproducible results.
○ Actual maximum or minimum sample concentration and loading volume are sample-dependent, and can vary with
different detergents or denaturants present in the sample. Therefore, sample loading conditions optimum –for –your
–particular -sample must be determined empirically in your laboratory.
○ Make sure to use the purest acetonitrile available. Even some HPLC-grade acetonitrile has been shown to contain
both high and low MW impurities which could complicate MS results.
○ GL-Tip SDB are disposable and should not be reused.
○ GL-Tip SDB should be stored in an environment with relatively constant temperature and humidity.
3. Solvent / Solution Preparation*
①Recommended Chemicals
・Highly pure acetonitrile (HPLC grade or higher)
・Trifluoroacetic acid (HPLC grade or higher)
②Solutions Required
・Solution A: Water (0.1% TFA) or 5% acetonitrile aqueous solution (0.1% TFA)
・Solution B: 80% acetonitrile aqueous solution(0.1%TFA)
*Note: Prepare solutions A & B on the day of the experiment
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