Leica PAULA Bedienungsanleitung

From Eye to Insight

Personal AUtomated Lab Assistant

Instructions for use

Leica Microsystems CMS GmbH •Instructions for use 11934164, Revision 1.0, 2018-06-15

PAUL A

Coyprights

Revision 1.0, published 2018-06-15 by:

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße 17-37

D-35578 Wetzlar (Germany)

http://www.leica-microsystems.com

Verantwortlich für den Inhalt:

Marketing CMS

Copyrights

All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustra-

tions (in whole or in part) by print, photocopy, microﬁlm or other method (including electronic systems) is not

allowed without express written permission from Leica Microsystems CMS GmbH.

The instructions contained in the following documentation reﬂect state-of-the-art technology. We have com-

piled the texts and illustrations as accurately as possible. Still, we are always grateful for comments and sug-

gestions regarding potential mistakes within this documentation.

The term „Windows“, “Apple”, and “Intel” may appear in the following text without further identiﬁcation. The

names of companies and products used herein may be trademarks of their respective owners.

The information in this manual is subject to modiﬁcation at any time and without notiﬁcation.

Contents

1. Important Notes about this Manual ................... 4

1.1 Text symbols and their meanings........................ 4

2. Intended Use........................................................... 6

3. Safety notes............................................................ 8

3.1 General safety notes ............................................. 8

3.2 Electrical Safety..................................................... 9

3.3 Ambient conditions.............................................. 10

3.4 Transport and storage......................................... 11

3.5 Notes on Cleaning ............................................... 11

3.6 Disposal ................................................................ 11

4. System Overview................................................. 12

4.1 Digital Imager ....................................................... 12

4.2 Intel NUC PC ......................................................... 13

5. Notes for the user................................................ 14

6. Local area network considerations................. 15

6.1 Integration into existing LAN ............................. 15

6.2 Local, ad-hoc (pairing) network ....................... 15

7. Supported control devices ................................ 16

8. Getting started...................................................... 17

8.1 Scope of Delivery:................................................ 17

8.2 Unpacking ............................................................. 18

8.3 Placement of the Digital Imager........................ 18

8.4 Connectivity set-up.............................................. 19

8.5 Software overview............................................... 23

8.6 Log-in ..................................................................... 26

9. User management................................................ 27

9.1 User management rules ..................................... 27

9.2 Create and modify accounts.............................. 28

10. Workﬂow............................................................... 29

10.1 Deﬁnition of specimen ........................................ 29

10.2 T-lapse .................................................................. 30

10.3 Cell based assays ............................................... 32

10.4 Data Management............................................... 34

11. Licensing of optional apps

for cell-based assays.......................................... 35

12. Trouble shooting .................................................. 36

13. Abbreviation and glossar................................... 37

14. Care........................................................................ 38

14.1 Cleaning................................................................. 38

14.2 Handling acids and bases .................................. 38

15. EC Declaration of Conformity............................ 39

1. Important notes about thIs manual

1. Important Notes about this Manual

Numbers in brackets, e.g. (1.2), refer to illustrations,

in the example to Fig. 1, item 2.

Numbers with an arrow, e.g. →p. 20, refer to a spe-

ciﬁc page of the manual.

WARNING indicates a hazard with a medium degree

of risk that, if not avoided, can result in death or seri-

ous injury.

CAUTION indicates a hazard with a low degree of

risk that, if not avoided, can result in minor or moder-

ate injury.

Caution!

In this manual, additional safety notes are indicated

with the triangle symbol shown here, and have a gray

background.

Caution! The instrument and accessories can be

damaged when operated incorrectly.

Caution!

These Instructions for Use are an essential component of the product; they must be read carefully before the

product is assembled, put into operation or used, and must be kept for later reference.

These Instructions for Use include important instructions and information related to the operating safety and

maintenance of the instrument.

(1.2)

→p. 20

WARNING

CAUTION

1.1 Text symbols and their meanings

1. Important notes about thIs manual

Warning of hazardous electrical voltage! Risk of

electrical shock!

Warning of electromagnetic ﬁeld

Warning of hazardous laser radiation – take appro-

priate safety precautions!

Instructions for disposing of the instrument, its ac-

cessories and consumables.

Explanatory note

Item not contained in all equipment conﬁgurations

Manufacturing date, e.g. 11 / 2011 for November 2011

China RoHS 50 years EFUP

(Environmentally friendly use period)

MM/YYYY

2. Intended Use

PAULA the Personal Automated Lab Assistant has been designed for cell culture routine cell checks and ba-

sic cell assays. It can be placed inside the cell culture incubator or outside on a clean bench or standard lab

bench. Up to 4 digital imagers can be combined to acquire simultaneously images and t-lapse series.

Directives of European Community (EC-Directives)

The PAULA system described here fulﬁlls the requirements of EU Directive 2014/53/EU concerning Radio

Equipment Devices and has been declared accordingly. The microscope also fulﬁlls the EU Directive 2014/35/

EU concerning the safety of electrical equipment and 2014/30/EU concerning electromagnetic.

The complete system does not conform to the requirements of an IVD device according to 98/79/EC According

to the VO1194/2012 regulation regarding the implementation of the Ecodesign Directive 2009/125/EC, micro-

scopes are considered special products. The light sources in this instrument are special lamps which are only

suitable for microscopes. They are not suitable for general lighting purposes or room lighting.

Reasonably foreseeable misuse

The following are prohibited:

Using PAULA system for any purpose not in accordance with the Declaration of Conformity (e.g. using it as

an in-vitro diagnostic product according to Council Directive 98/79/EC or as a medical product according to

Council Directive 93/42/EEC)

Cleaning PAULA ( digital imager and NUC PC) in a way other than speciﬁed in the manual

Using another power supply that Leica Microsystems CMS GmbH has not provided or permitted

Allowing unauthorized personnel to open the device

Installing PAULA in a way other that Leica Microsystems CMS GmbH has speciﬁed in this manual

Using cables that Leica Microsystems CMS GmbH has not provided or permitted

Using PAULA in other environmental conditions than speciﬁed in this manual

Using combinations with non-Leica components that go beyond the scope of the manual

To put the power supplies, Intel NUC PC or Monitor/Tablet in the incubator

Caution!

The manufacturer assumes no liability for damage caused by, or any risks arising from, using the microscope

for other purposes than those for which they are intended or not using them within the speciﬁcations of Leica

Microsystems CMS GmbH.

In such cases, the Declaration of Conformity shall be invalid.

2. Intended Use

WARNING

Notes on handling laser devices

In the standard design without additional laser safety precautions, the microscopes are not suitable for cou-

pling laser radiation (such as to the camera ports), since this radiation poses a hazard for the user (eye injury

in particular).

For use of the microscope with lasers, Leica Microsystems CMS GmbH offers special microscope variants

with additional safety features. Laser couplings require corresponding safety devices that have to be inspect-

ed and installed by trained personnel.

For further information, please contact your authorized Leica Microsystems CMS GmbH representative.

Due to the special sources of danger of the laser system, only those persons may use the systems who have

received special training in working with the system, observing the speciﬁc hazards of a laser system.

3. safety notes

3. Safety notes

3.1 General safety notes

Caution!

PAULA is a device of protection class 3 which is constructed and tested in accordance with EN 61010-1/IEC

61010-1, Safety requirements for electrical equipment for measurement, control and laboratory use and EN

61326-1/ IEC 61326-1, Electrical equipment for measurement, control and laboratory use – EMC requirements.

It also meets the requirements ETSI EN 301489, Electro Magnetic Compatibility (EMC) standard for radio equip-

ment and services Part 1 and Part 17, ETSI EN303446-1 ElectroMagnetic Compatibility (EMC) standard for com-

bined and/or integrated radio and non-radio equipment. – EMC requirements.

It also complies with EN 62471 / IEC 62471, Photo-biological safety of lamps and lamp systems, and belongs to

the exempt level (no photobiological risk).

Attention!

In order to maintain this condition and to ensure safe operation, the user must follow the instructions and

warnings contained in this user manual.

The instruments and accessories described in this manual have been safety-tested and checked for possible

hazards. The responsible Leica afﬁliate or the main plant in Wetzlar must be consulted whenever the instru-

ment is altered, modiﬁed or used in conjunction with non-Leica components that are outside of the scope of

this manual.

It is not allowed to put the power supplies, PC, or monitor/tablet into the incubator. Only PAULA is appropriate

for this application.

Caution!

Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims and

product liability!

In addition to this manual, the safety notes of the other manuals provided must be observed!

Only personnel who has been instructed by authorized Leica personnel may operate the microscope!

PAULAs electrical accessory components are not protected against water. Water can cause electric shock.

Do not plug in or unplug data lines and control circuits unless the instrument is switched off; otherwise, the

instrument may be damaged.

3. safety notes

3.2 Electrical Safety

Power the PAULA system via an external power supply. Only use the (LPS-certiﬁed) Power Supply FSP060-

DIBAN2 or Mean Well GST60A12-P1J

For indoor use only.

General speciﬁcations PAULA digital imager (NUC PC excluded):

Supply voltage: 12V DC

Power consumption: max. 30VA

Storage temperature: -20°C ... +70°C

Operating temperature: +15°C ... +37°C

Relative humidity: 10% – 90%,

up to 40°C non-condensing

Technical Data of the external Power Supply FSP060-DIBAN2 and Mean Well GST60A12-P1J for PAULA system

Supply Voltage: 100-240 V AC

Frequency: 50-60 Hz

Power: max. 60 W

Output voltage: 12 V DC

Output current: max. 5,0A

Ambient temperature: 0°C – 40°C

Storage temperature: -20 to +70°C

Relative humidity: 10% – 90%, up to 40°C non-condensing

Power NUC6i7KYK via an external power supply. Only use the (LPS-certiﬁed) Power Supply Delta Electronics

ADP-120RH D

General speciﬁcations NUC6i7KYK PC

Supply voltage: 19V DC

Power consumption: max. 90VA

Storage temperature: -40°C ... +70°C

Operating temperature: +0°C ... +35°C

Technical Data of the external Power Supply (Delta Electronics ADP-120 RH D) for NUC

Supply Voltage: 100-240 V AC

Frequency: 50-60 Hz

Power: max. 120 W

Output voltage: 19 V DC

Output current: max. 3,43A

Ambient temperature: 0°C – 40°C

Storage temperature: -30 to +70°C

For detailed safety notes concerning the NUC PC please consult instruction for use of the device.

WARNING

Use the original power supply only. Other power supplies must not be used. If the original power supply fails or

is damaged, it must be replaced. Repair is not permitted. Original power supplies are available from your Leica

branch ofﬁce or Leica dealer.

Placing the NUC PC into an incubator is not allowed.

3. safety notes

Caution!

The electrical components are not protected against water and liquid solutions. Water can cause electric

shock.

It is not allowed to keep PAULA placed inside a cell culture incubator while running a sterilization procedure.

Electrical components must be assembled at least 10 cm from the wall and away from ﬂammable substances.

The PAULA device comes standard with a NUC with a W-LAN module integrated by the PC manufacturer.

Please note the documentation supplied with the NUC (technical data, safety information, regulatory informa-

tion, Quick Guide, etc.)

Further information can be found on the homepage of the manufacturer.

3.3 Ambient conditions

Temperature: 15°C – 37°C

Relative humidity: 10-90% up to 40°C non-condensing

Caution!

Do not use the device in altitudes exceeding 2000 m ASL/NL.Transport and storage in a range of -10°–70°C and

at a humidity not exceeding 80% (non-condensing). Make sure that a free air circulation.

WARNING

By deﬁnition, the main circuit breaker of this instrument is the connection between the power supply and the

socket. The user must ensure unobstructed access to the main circuit breaker at all times.

Caution!

Do not use this instrument near sources of high electromagnetic radiation (for example, unshielded, intention-

ally operated ultra-high frequency sources), because these can disrupt proper operation.

We recommend assessing the electromagnetic environment before operation of this instrument and then giv-

ing corresponding instructions.

This instrument has been developed and tested in accordance with CISPR 11 Class B

Caution!

Fully equipped, PAULA ( digital imager without NUC PC) weighs more than 6.5 kg. For placing the imager inside

the incubator the user has to make sure that the shelves are capable of holding this weight.

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