MyGo Mini Bedienungsanleitung

USER GUIDE

PERSONAL PRECISION

MyGo

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WELCOME

Thank you for choosing the MyGo Mini.

The MyGo Mini is the world’s most compact

real-time PCR instrument, with no moving parts

providing silent operation and maximum reliability.

With outstanding precision, it is capable of providing

great results over a broad range of applications.

Personal precision... SETUP

Learn how to install your

MyGo Mini - from setting it

up on your workbench, to

configuring the instrument

and installing the software.

TIPS

Learn some useful tips when

using your instrument.

EXPERIMENT

Learn the basics of setting

up an experiment on your

MyGo Mini.

ANALYSIS

Learn how to easily

analyse your results.

EXPORT

Learn how to export your data

for use with other software.

MAINTENANCE

Learn how to look after

your MyGo Mini.

TROUBLESHOOTING

If you ever have a problem

with your instrument, learn

how to troubleshoot it here.

CONTENT

This quick start guide will teach you what you need

to know to start running your MyGo Mini. It describes

everything from opening and closing the lid, to data

analysis for your qPCR experiments.

Contents include...

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1. MYGO MINI QPCR INSTRUMENT

2. MYGO MINI USER GUIDE

3. POWER SUPPLY UNIT AND MAINS CABLE

4. ETHERNET CABLE

5. USB DRIVE

6. USB DRIVE EXTENSION CABLE

SETUP

INSTALLING YOUR MYGO MINI

You should have the following items in the MyGo Mini box:

Please keep the box the MyGo Mini came in, in case you need to

protect your MyGo Mini during storage or shipment in future.

Heatsink

Lid

Foot

Display LEDs

LID

The lid of the MyGo Mini

can be removed from the

instrument and must be

attached at all times when

not in use. To remove the

lid push down and turn

anticlockwise. To attach

the lid, first line up the lugs

on the lid with the grooves

on the mount plate as

shown below. Once aligned

press down, and then turn

clockwise to secure until you

feel a hard stop.

If the instrument is

connected to power the

instrument will say “Lid on”

and “Lid off” when you attach

and remove the heated lid.

The instrument will display

green when the lid is fitted,

and display pulsating yellow

when the lid is removed.

The lid may get warm during

operation; this is normal.

HEATSINK

This is the outer body of

the instrument and enables

efficient cooling of the

system during thermal

cycling. Ensure that there

is free airflow around the

instrument - do not cover

or wrap the instrument,

and keep it at least 5 cm

away from objects or walls.

FOOT

The foot should always be

placed facing down on your

worktop during operation.

Grooves

Lugs

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USB PORT

MYGO MINI CONNECTIONS

If you turn your MyGo Mini upside down you will see

three connections as shown below:

Insert the USB drive here

to run an experiment from

the USB drive, or to update

firmware using a USB drive.

You can use the supplied USB

drive extension cable.

This is for connecting your

MyGo Mini to your LAN or

computer.

USB Port

Ethernet Port

Power

ETHERNET PORT

POWER

This is to connect your MyGo Mini to the provided power supply unit.

Connect one end of

the ethernet cable to

the instrument and the

other end to a LAN port,

or directly to your computer.

Once your MyGo Mini is

connected to a LAN, you can

connect to your instrument via

Wi-Fi if your network supports it.

POWERING ON YOUR MYGO MINI

Connect the power supply to the MyGo Mini, place

the MyGo Mini on your lab bench, and then connect

AC power to the power supply unit. Your instrument

will now turn on after a few seconds. The display LEDs

will light up multi-coloured, and then turn green if a lid

is attached, or yellow if not. Your MyGo Mini is ready

to run.

CONNECTING

YOUR MYGO MINI

TO A NETWORK,

PC OR LAPTOP

A summary of these connections

is shown on the following page...

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MyGo Mini Connection Modes

1. Direct network connection

2. Local area network connection

3. USB drive connection

MYGO MINI LIGHTS AND SOUNDS

The MyGo Mini uses display LEDs and sounds to

help you understand what the instrument is doing.

Here is a summary of these display LEDs and sounds:

DISPLAY LEDs

SOUNDS

Green Idle / Ready to run

Yellow Lid off

Blue Experiment is starting/

cooling down

Blue Running, low temperature

Pink Running, medium temperature

Red Running, high temperature

Multi-Coloured Experiment complete

Red-Flashing Fault detected

“Lid On” The lid has been attached

“Lid Off” The lid has been removed

“Analysis Starting” An experiment has begun

“Analysis Complete” An experiment has been

completed

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INSTALLING YOUR

MYGO MINI SOFTWARE

Your MyGo USB drive contains software for Windows,

Mac OS X and Linux operating systems. Please open

the software le matching your chosen operating

system. The latest version of the software can also be

downloaded from our website mygopcr.com.

WINDOWS

Double-click on the

windows installer and follow

the on-screen instructions

to install your MyGo Mini

software on Windows.

MAC OS X

Double-click on the

disk image and follow

the on-screen instructions

to install your MyGo Mini

software on your Mac PC.

CONFIGURING YOUR MYGO MINI

With your MyGo Mini software open and your

MyGo Mini connected to the network please connect

to your MyGo Mini Instrument.

Please select Configuration

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To add your new MyGo Mini

select the Add button. You

will now be presented with

a list of available instruments.

Please select MyGo Mini

Double-click on the

instrument you wish to

connect to, or press Select

with the instrument selected.

The instrument will now

be added to the list of

Registered Instruments

for use in the software.

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Intensity (RFU)

Cycle

TIPS

Here are some great tips to keep in mind whilst running your

MyGo instrument.

1. LID GETS WARM

2. DO NOT LEAVE THE LID OFF

3. SPIN YOUR TUBES

4. REMOVE ALL BUBBLES

5. KEEP YOUR LAB CLEAN

6. KEEP YOUR INSTRUMENT CLEAN

7. PC SETTINGS

LID GETS WARM

The MyGo Mini lid can get warm during operation, this is

completely normal.

SPIN YOUR TUBES

This will ensure all well contents are at the bottom of the

wells and will improve performance.

REMOVE ALL BUBBLES

Bubbles can cause optical artefacts as shown in the graph

below. Ensure that no bubbles are present in reaction volumes.

KEEP YOUR LAB CLEAN

Please keep your work space clean including all lab equipment

like surfaces, pipettes, and tube racks. This will keep the

instrument clean and help maintain good results.

KEEP YOUR INSTRUMENT CLEAN

We recommend a routine cleaning of your instrument. To do

so follow the cleaning guide in the Maintenance section.

PC SETTINGS

Please disable all power saving settings e.g. sleep and hibernate.

DO NOT LEAVE THE LID OFF

If the lid is off dust may fall into the wells and affect the

performance of your instrument.

Artefact

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CREATE A NEW EXPERIMENT

EXPERIMENT

This section will teach you everything you need to know

to get started with MyGo Mini experiments. You will

learn how to create, save, open and close experiments.

You will also learn how to set up an experiment including

thermal prole, sample information and optical settings.

Finally we will show you how to run your new experiment

from the software and from the USB drive provided.

Below is the MyGo mini home screen.

Click New to create

a new experiment.

EXPERIMENT SUMMARY

Once you have created a new experiment the

Experiment Summary will be displayed as shown below:

By default the experiment

name will be “New

Experiment” with a date

and time stamp, which

can be edited.

The summary will give

you information about the

instrument you are running,

the settings chosen and any

useful notes you choose to

add about your experiment.

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The Files of Type drop down

menu gives you the option to

open an experiment normally

or as a template that can be

used for a new run.

OPEN AN EXPERIMENT

By selecting Open you can open an experiment

as shown below:

FILES OF TYPE

EXPERIMENT FILE

An experiment file will contain

all the data and analyses but

can not be re-run.

To open as an Experiment

File select the first option

shown on the opposite page.

EXPERIMENT FILE

AS TEMPLATE

Opening an experiment file

as a template allows you to

use the same profile, samples,

settings and analyses to

re-run a previous experiment,

generating new data. When an

experiment file is opened as

a template, it has the same:

1. Temperature profile.

2. Optical settings.

3. Samples and targets.

4. Analyses, including settings.

If you wish to open an

Experiment File as Template

select the second option.

USB RUN

To open a USB run navigate to the USB location using the

Open dialog window shown to the left. Select Experiment File

in the Files of Type drop down menu and then double click on

the USB run.

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