Qiagen PyroMark Q96 ID Bedienungsanleitung

Version 5 January 2016

Sample & Assay Technologies

PyroMark®Q96 ID User Manual

Contents

PyroMark Q96 ID User Manual 01/2016 Contents-1

Contents

1Safety Information 1-1

1.1 Proper use 1-1

1.2 Electrical safety 1-2

1.3 Chemical hazards 1-3

1.4 Mechanical hazards 1-3

1.5 Heat hazards 1-4

1.6 Symbols used on the PyroMark Q96 ID 1-4

2Introduction 2-1

2.1 About this user manual 2-1

2.2 General information 2-2

2.2.1 Technical assistance 2-2

2.2.2 Policy statement 2-2

2.2.3 Version management 2-2

2.3 Intended use of the PyroMark Q96 ID 2-3

2.3.1 Requirements for PyroMark Q96 ID users 2-4

3General Description 3-1

3.1 PyroMark Q96 ID definitions 3-1

3.2 Pyrosequencing principle 3-1

3.3 PyroMark Q96 ID principle 3-3

3.4 External features of the PyroMark Q96 ID 3-5

3.5 Internal features of the PyroMark Q96 ID 3-6

3.6 Process chamber 3-6

3.7 Dispensing unit 3-6

3.8 Controls and indicators 3-7

3.8.1 Front panel 3-7

Contents

Contents-2 PyroMark Q96 ID User Manual 01/2016

3.8.2 Rear panel 3-7

3.9 Computer and software 3-8

4Installation Procedures 4-1

4.1 Instrument delivery and installation 4-1

4.2 Requirements 4-1

4.3 Moving the PyroMark Q96 ID 4-3

4.4 Reinstalling the PyroMark Q96 ID: Stand alone 4-4

4.5 Reinstalling the PyroMark Q96 ID: Network installation 4-4

4.6 Connecting mains power 4-5

4.7 Setting up network properties 4-5

4.7.1 Used IP addresses f or instrument and computer 4-5

5Operating Procedures 5-1

5.1 Required items 5-1

5.2 Starting the system 5-1

5.2.1 Starting the PyroMark Q96 ID 5-1

5.2.2 Starting the PyroMark Q96 Software 5-2

5.3 Preparing a run 5-2

5.3.1 Setting up an assay 5-2

5.3.2 Setting up a run 5-3

5.3.3 Managing instrument parameters 5-4

5.4 Sample preparation 5-6

5.4.1 Assembly of the PyroMark Q96 Vacuum Workstation 5-7

5.4.2 DNA amplification 5-7

5.4.3 Immobilization of the PCR product to beads 5-8

5.4.4 Strand separation 5-9

5.4.5 Annealing of sequencing primer to samples 5-14

5.4.6 Reagent preparation 5-14

5.5 Run 5-15

5.5.1 Loading the PyroMark Q96 Plate Low 5-15

Contents

PyroMark Q96 ID User Manual 01/2016 Contents-3

5.5.2 Loading the reagent cartridge 5-16

5.5.3 Starting the run 5-17

5.5.4 Monitoring the run 5-17

5.6 Post-run procedures 5-18

5.6.1 Analyzing the run 5-18

5.6.2 Viewing the analysis results 5-19

5.6.3 Analysis reports 5-22

5.7 Finishing work 5-23

5.7.1 After a run is finished 5-23

5.7.2Leaving the instrument on 5-23

5.7.3 Exiting PyroMark Q96 Software 5-24

5.7.4 Cleaning the instrument 5-24

5.7.5 Emptying the waste container and troughs 5-24

6PyroMark Q96 Software 6-1

6.1 System requirements 6-1

6.1.1 Operator’s and office computers 6-1

6.1.2 Printer 6-1

6.1.3 Data storage device 6-1

6.2 Installing PyroMark Q96 Software 6-1

6.3 Uninstalling PyroMark Q96 Software 6-2

6.4 Upgr ading from PyroMark Q96 ID Sof tware 6-2

6.4.1 Installing the PyroMark Data Converter Tool 6-3

6.4.2 Using the PyroMark Data Converter Tool 6-3

6.5 Data backup 6-5

6.6 Installation testing 6-5

7Maintenance 7-1

7.1 Shutdown procedure 7-2

7.2 Routine maintenance of the PyroMark Q96 ID 7-2

7.3 Cleaning the lens array 7-3

Contents

Contents-4 PyroMark Q96 ID User Manual 01/2016

7.4 Cleaning the heating block 7-3

7.5 Changing the fuses 7-4

7.6 Cleaning and testing the reagent cartridge 7-5

7.6.1 Standard cleaning procedure 7-5

7.6.2 Clogged cartridge cleaning procedure 7-6

7.6.3 Cartridge function check 7-6

7.7 Cleaning the PyroMark Q96 Vacuum Workstation 7-8

7.8 Testing and replacing the filter probes 7-9

7.8.1 Function test for filter probes 7-9

7.8.2 Replacing filter probes 7-10

7.9 Replacing the tubing 7-10

8Troubleshooting 8-1

8.1 Unexpected results 8-1

8.2 Sequence errors 8-2

8.3 Peak errors 8-3

8.4 Instrument-related errors 8-5

8.5 Vacuum workstation-related errors 8-6

9Glossary 9-1

Appendix A A-1

Technical data A-1

Environmental conditions A-1

Waste Electrical and Electronic Equipment (WEEE) A-4

FCC declaration A-5

Appendix B B-1

Assay design and validation B-1

Contents

PyroMark Q96 ID User Manual 01/2016 Contents-5

Appendix C C-1

Liability clause C-1

Appendix D D-1

Informations de sécurité D-1

Appendix E E-1

Sicherheitshinweise E-1

Index Index-1

Contents

Contents-6 PyroMark Q96 ID User Manual 01/2016

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Safety Information

PyroMark Q96 ID User Manual 01/2016 1-1

1Safety Information

Before using the PyroMark Q96 ID, it is essential that you

read this user manual carefully and pay particular attention

to the safety information. The instructions and safety

information in the user manual must be followed to ensure

safe operation of the instrument and to maintain the

instrument in a safe condition.

The following types of safety information appear throughout

this manual.

WARNING

The term WARNING is used to inform you about situations

that could result in personal injury to you or other

persons.

Details about these circumstances are given in a box like

this one.

CAUTION

The term CAUTION is used to inform you about situations

that could result in damage to the instrument or other

equipment.

Details about these circumstances are given in a box like

this one.

The advice given in this manual is intended to supplement,

not supersede, the normal safety requirements prevailing in

the user’s country.

1.1 Proper use

WARNING/

CAUTION

Risk of personal injury and material damage

[W1]

Improper use of the PyroMark Q96 ID may cause personal

injuries or damage to the instrument.

The PyroMark Q96 ID must only be operated by qualified

personnel who have been appropriately trained.

Servicing of the PyroMark Q96 ID must only be performed

by QIAGEN Field Service Specialists.

Safety Information

1-2 PyroMark Q96 ID User Manual 01/2016

WARNING/

CAUTION

Risk of personal injury and material damage

[W2]

The PyroMark Q96 ID is too heavy to be lifted by one

person. To avoid personal injury or damage to the

instrument, do not lift the instrument alone.

Perform the maintenance as described in Section 7. QIAGEN

charges for repairs that are required due to incorrect

maintenance.

1.2 Electrical safety

Disconnect the line power cord from the power outlet before

servicing.

WARNING

Electrical hazard

[W3]

Any interruption of the protective conductor (earth/ground

lead) inside or outside the instrument or disconnection of

the protective conductor terminal is likely to make the

instrument dangerous.

Intentional interruption is prohibited.

Lethal voltages inside the instrument

When the instrument is connected to line power, terminals

may be live, and opening covers or removing parts is likely

to expose live parts.

To ensure satisfactory and safe operation of the PyroMark

Q96 ID and PyroMark Q96 Vacuum Workstation, follow the

advice below:

The line power cord must be connected to a line power

outlet that has a protective conductor (earth/ground)

Keep mains plugs easily accessible in case the

equipment needs to be disconnected quickly from mains

power

Use only power supplies and cords supplied with the

instrument

When replacing the mains fuse, replace only with the

type and current rating specified on the rating label

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