Singer Instruments PhenoBooth Bedienungsanleitung
USER GUIDE v1.5
·Remove plate lids before imaging. If lids are in place when the input
tray is closed, the machine will crash and may be damaged.
·Keep your hands clear while the input tray is moving. Catching your
hands in the input tray may cause injury.
·Make sure the PhenoSuite and PhenoBooth light settings match.
When capturing an image in PhenoSuite, be sure to match the
settings on the PhenoBooth’s blackout slider.
·Ensure power supply is the correct way round. Plugging the power
supply into the PhenoSuite upsidedown will cause damage to the
machine. See p.9 for details.
·Avoid plugging the USB 3.0 cable in slowly. A common error with all
USB 3.0 devices is that they are recognised as USB 2.0. This happens
when the USB plug is inserted too slowly.
BEFORE YOU START!
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Before you start!
Introduction
OUT OF THE BOX
Anatomy & features
Unboxing & installation
LET’S START!
PhenoBooth operation
PhenoSuite operation - New
project
Image acquisition
COLONY COUNTING PROJECT
Pre-processing
Detection
Filtering
SGA PROJECT
Pre-processing
Detection
Filtering
Analysis
Visualisation
RCP PROJECT
Pre-processing & Detection
Filtering
Stinger Export
ROTOR HDA / The Stinger
BEST PRACTICE
PhenoSuite icons
Feedback light
Batch processing
Export
History plug-in
Add new plate run
Change heat map colours
Using filters
Notes
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PHENOBOOTH™
INTRODUCTION
The PhenoBooth is a fast, semi-automated plate reader for imaging
and analysis of cell colonies. The PhenoBooth can image standard
SBS plates, Petri dishes and Singer PlusPlates.
USER GUIDE
Follow these instructions to get the most out of the PhenoBooth.
This guide outlines getting started and general operation, as well as
PhenoSuite tutorials for each package.
DISCLAIMER
At Singer Instruments, we are constantly seeking to improve our
products and adapt them to the requirements of modern research
techniques and testing methods. This involves modification to
the mechanical structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this user guide,
including all specifications are subject to change without notice.
To download the latest version of this user guide please visit:
singerinstruments.zendesk.com
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OUT OF THE BOX
Find out what comes with the PhenoBooth. We’ll take you
through the steps involved in unboxing and assembling
ready for the exciting world of colony imaging!
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ANATOMY & FEATURES
Input tray
function button
Filter tray
PHENOBOOTH
Input tray Feedback light Blackout screen
USB 3.0 socket
Power socket
Power switch
Capture
function button
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PHENOSUITE
Menu bar Stage selection
General tools
Plug-in tools
Plug-insLast task completed Current plate Plug-in
information
Plate preview
Plate information Progress bar
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UNBOXING & INSTALLATION
IN THE BOX
1. PhenoBooth
2. USB 3.0 Cable
3. Power Supply
4. 90mm Petri Adapter (Optional Extra)
1. 2.
3.
4.
MINIMUM REQUIREMENTS
· Intel Core i5 2.4GHz
· 4GB of RAM
· 512GB hard disk space
· Microsoft Windows 7 (64-bit)/Windows 8.1 (64-bit)
· USB 3.0 port
·
INSTALLATION STEPS
1. Go to http://si-deployment.azurewebsites.net/install/publish.htm
2. Click install to download PhenoSuite
3. Install PhenoSuite
4. After installation, PhenoSuite will start automatically and ask for
organisation name and licence code
5. Enter the organisation name and licence code that came with your
PhenoBooth packing documents and click Activate
6. After activation PhenoSuite will automatically download and install
dependencies
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· Insert the USB cable into the PhenoBooth USB 3.0
socket and to the your computer.
· You’re now good to go!
· Carefully remove the PhenoBooth from the box and
stand on your workspace.
· HEAVY: May require 2 people.
· Insert power supply and turn on the power switch.
· WARNING: Make sure the arrow diagram is on top
of the cable. Inserting the power supply upsidedown
will cause damage to the PhenoBooth.
· Download and install PhenoSuite onto your chosen
computer. Follow the steps on page 8.
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UNBOXING & INSTALLATION
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