Star Lab ErgoOne E Bedienungsanleitung

STARLAB International GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

info@starlab.de

www.starlabgroup.com

STARLAB GmbH

Neuer Höltigbaum 38

22143 Hamburg

Germany

T: +49 (0)40 675 99 39 0

F: +49 (0)40 675 99 39 20

info@starlab.de

www.starlab.de

STARLAB (UK), Ltd

5 Tanners Drive

Milton Keynes MK14 5BU

United Kingdom

T: +44 (0)1908 283800

F: +44 (0)1908 283802

infoline@starlab.co.uk

www.starlab.co.uk

STARLAB FRANCE

30 Rue Jean Rostand

91400 Orsay

France

T: +33 (0)1 60 1371 70

F: +33 (0)1 69 4148 65

info@starlab-france.com

www.starlab-france.com

STARLAB S.r.l.

Via Pinturicchio 1

20133 Milano

Italy

T: +39 (0)2 7020 1040

F: +39 (0)2 7020 1033

info@starlabitalia.it

www.starlabitalia.it

www.starlabgroup.com

User Manual · Bedienungsanleitung · Mode d‘emploi

Istruzioni per l‘uso · Instrucciones de manejo

  

9974 79 • Printed in Germany • 21/0115/1

STARLAB_ErgoOne E SC_6.5 Rücken_2014 12 09_v2.indd 1 09.12.2014 13:50:33

EC Declaration of Conformity

STARLAB certifies that all variants of

ErgoOne®E

including charging adapter

has been manufactured and inspected in accordance

with the following European standards:

2004/108/EC: EN 61326-1:2006

2006/95/EC: EN 61010-1:2010 - EN 60950-1:2006+A11:2009

As an ISO 9001 and ISO 13485 certified company,

our working procedures and processes are checked

and audited regularly

and the quality of our products is continuously monitored.

STARLAB International GmbH · Neuer Höltigbaum 38 · 22143 Hamburg

www.starlabgroup.com

the content of this publication is correct. STARLAB can not take responsibility for any errors or omissions.

STARLAB_ErgoOne E SC_6.5 Rücken_2014 12 09_v2.indd 2 09.12.2014 13:50:33

English

TABLE OF CONTENTS

Page

Safety Instructions 4

Functions and Limitations of Use 5

Operating Exclusions 5

Disposal 5

Operating Elements 6

The First Steps 7

Setting the Volume 8

Setting the Aspiration and Discharging Speed 9

Correct Pipetting 10

The Pipetting Programs 11

PIP mode 12

PIPmix mode 14

revPIP mode 16

Electrophoresis (GEL) mode 18

DISP mode 20

Checking the Volume 22

Accuracy Table 23

Easy Calibration (adjustment) 24

Autoclaving 26

Reference Run (rEF) 26

Servicing and Cleaning 27

Charging and Replacing the Battery 29

Battery Regeneration Function 30

Troubleshooting 31

Ordering Information · Accessories · Spare Parts 32

Repairs and Warranty Information 34

ErgoOne E · User Manual · 3

Table of Contents

Please read the following carefully!

This instrument may sometimes be used with hazardous materials, operations, and equipment. It is be-

yond the scope of this manual to address all of the potential safety problems associated with its use in

such applications. It is the responsibility of the user of this pipette to consult and establish appropriate

safety and health practices and determine the applicability of regulatory limitations prior to use.

SAFETY INSTRUCTIONS

Improper use of the instrument or the batteries (short circuit, mechanical damage,

overheating, incorrect AC adapter, etc.) can lead to battery explosion.

WARNING!

1. Every user must read and understand this

operating manual prior to using the instrument

and observe these instructions during use.

2. Follow general instructions for hazard preven-

tion and safety instructions; e.g., wear protec-

tive clothing, eye protection and gloves.

When working with infectious or other hazar-

dous samples, all appropriate regulations and

precautions must be followed.

3. Observe all specifications provided by reagent

manufacturers.

4. Never use the instrument in an atmosphere

with a danger of explosion. Highly flammable

liquids must not be pipetted.

5. Only use the instrument for pipetting liquids

that conform to the specifications defined in

the operating exclusions and limitations (see

page 5). If in doubt, contact the manufacturer

or supplier.

6. Always use the instrument in such a way that

neither the user nor any other person is

endangered. Avoid splashes. Only use suitable

vessels.

7. Avoid touching the tip orifices when working

with hazardous samples.

8. Never use force on the instrument!

9. Use only original spare parts. Do not attempt

to make any technical alterations. Do not

dismantle the instrument any further than is

described in the operating manual!

10. Before use check the instrument for visible

damages. If there is a sign of a potential

malfunction (e.g., piston difficult to move,

mechanically damaged connections), immedi-

ately stop titrating. Consult the 'Troubleshoot-

ing' section of this manual (see page 31), and

contact the manufacturer if needed.

11. The original battery must not be replaced with

non rechargeable batteries or rechargeable

batteries of other manufacturers.

12. To charge the NiMH battery pack, use only the

original AC adapter.

13. The AC adapter has to be protected against

moisture and must be used only for this instru-

ment.

14. Dispose of batteries only when discharged and

according to applicable regulations.

4· ErgoOne E · User Manual

English

FUNCTIONS AND LIMITATIONS OF USE

The ErgoOne®E is a microprocessor-controlled,

battery-operated piston-stroke pipette which uses

the air-displacement principle for the pipetting of

aqueous solutions with an average density and

viscosity.

When the instrument is used properly, the sample

only comes into contact with the tip and not with

the ErgoOne®E.

The ErgoOne®E is intended for the pipetting of

liquids within the following limitations:

– Temperature of both the instrument and solution

should be between 15 °C and 40 °C (59 °F and

104 °F) for the instrument and the reagent. Con-

sult the manufacturer for use in temperatures

outside of this range.

– Vapor pressure up to 500 mbar

– Viscosity: 260 mPa s (260 cps)

Battery

Nickel-metal hydride battery with 3 cylindrical

individual cells with size AAA,

3.6 V, 700 mAh

AC adapter

Output voltage 6.5 V DC, 200 mA

Limitations of use

Operating exclusions Battery and AC adapter speciﬁcations

Viscous and highly adhesive liquids may impair

volumetric accuracy. Volumetric accuracy may also

be impaired when pipetting liquids that differ from

ambient temperature by more than ± 5 °C / 41 °F.

Operating Limitations

The user has to ensure the compatibility of the

instrument with the intended application.

Never use the instrument for pipetting liquids

that react adversely with polypropylene (PP: shaft

and tips), polycarbonate/polybutyleneterephthalate

(PC/PBT: casing). Avoid reactive vapors due to the

danger of corrosion.

The handle is not autoclavable.

DISPOSAL

The adjoining symbol means that storage batteries and electronic devices

must be disposed of separately from household waste (mixed municipal

waste) at the end of their service life.

- According to the Directive 2002/96/EC of the European Par-

liament and of the Council on Waste Electrical and Electronic

Equipment (WEEE) of 27 January 2003, electronic equipment

requires disposal according to the relevant national disposal

regulations.

- Batteries contain substances that can have harmful effects on the environ-

ment and human health. Therefore according to the Directive 2006/66/

EC of the European Parliament and the Council on Waste Batteries of 6

September 2006 batteries require disposal according to the relevant national

disposal regulations. Dispose of batteries only when completely discharged.

Do not short-circuit the battery to discharge it!

WARNING!

ErgoOne E · User Manual · 5

Functions and Limitations of Unse

Display

Program keys

Tip ejection key

Handgrip

Pipette shaft

Tip cone

Pipetting key

Charging jack

OPERATING ELEMENTS

selection

key

Entry confir-

mation/

Power 'On'*

Pipetting key

UP key (+)

DOWN key (-)

The ErgoOne®E is a microprocessor-controlled, battery-

operated, piston-stroke pipette, which has been opti-

mized for ergonomic operation

and ease of use.

Battery compartment

Finger rest The ErgoOne®E rests

easily and ergono-

mically in your hand.

The finger rest is height

adjustable with a screw,

which allows fine-tuning of

the hand position for effort-

less operation of the function

buttons.

*) The instrument is activated by pressing the ENTER key.

Then press the pipetting key, and the instrument is ready

for pipetting.

The ErgoOne®E shuts off 10 min after the last instru-

ment operation (Auto-Power-Off).

Key Functions

6· ErgoOne E · User Manual

English

c) Replace the battery compartment.

1. Insert the

battery

Is everything in the package?

Confirm that your package includes: ErgoOne®E pipette, battery, AC adapter

with battery charging cable, an AC adapter for UK, silicone oil and operating

manual.

a) Open the cover of the battery compart-

ment.

b) Ensure that the plug for the battery is firmly

connected to the pipette. Insert the battery.

2. Activate the

instrument

The ErgoOne®E automati-

cally requests a reference run

directly after the battery is

inserted. After the pipetting

key is pressed, the reference

run is carried out and the

instrument is now ready for

pipetting.

Battery charge

indicator

Up arrow,

indicating

aspiration

Pipetting mode

Volume setting

The display shows the

standard factory setting

(pipetting mode/PIP); and

the nominal volume (for

example, 200.0 µl). Default

aspiration and discharging

speeds are at maximum.

The adjustment of volume

and speed is described on

the following pages.

THE FIRST STEPS

Initializing the ErgoOne®E

The First Steps

ErgoOne E · User Manual · 7

–

Press the UP key (+) to

increase the volume. Press-

ing and holding the key

down accelerates the rate

of change. 'VOL' continues

to blink.

Press the ENTER key.

The display now shows the

new volume setting, in this

case, 102.8 µl in the PIP

mode.

2. Change the volume

3. Conﬁrm volume

setting

The volume for the ErgoOne®E is set at the factory to the nominal volume of the instru-

ment and can be changed quickly and easily.

Press the DOWN arrow key

(-) to reduce the volume.

Pressing and holding the key

down accellerates the rate

of change. 'VOL' continues

to blink.

Reduce volume

Press either the UP or DOWN

key to activate volume selec-

tion 'VOL' blinks.

1. Activate volume

setting

Increase volume

SETTING THE VOLUME

What to do How to do it Keys to press Display readout

By pressing the MENU key any procedure can be cancelled! The display then moves to

the next setting or back to the initial display (depending on actual selection.)

IMPORTANT:

8· ErgoOne E · User Manual

–

English

Press the MENU key once

to bring up the aspiration

speed menu. 'Speed' blinks.

Press either the UP or DOWN

key to select the desired

speed (in this case, level 5).

'Speed' continues to blink.

Press the ENTER key. The

display returns to the start

position for the current

pipetting mode (in this case,

the standard PIP mode).

1. Bring up the menu

2. Change the

aspiration speed

3. Conﬁrm speed level

The aspiration and discharging speeds can be individually adjusted. When the menu is called up, the last

speed setting is shown. Five speed levels are available.

Press the MENU key twice

to bring up the discharging

speed menu. 'Speed' blinks.

Press either the UP or DOWN

key to select the desired

speed (in this case, level 2).

'Speed' continues to blink.

Press the ENTER key. The

display returns to the start

position for the current pipet-

ting mode (in this case, the

standard PIP mode).

1. Bring up the menu

2. Change the

discharging speed

3. Conﬁrm speed level

Setting the aspiration speed

Setting the discharging speed

SETTING THE ASPIRATION AND DISCHARGING SPEED

What to do How to do it Keys to press Display readout

Setting the Aspiration and Discharging Speed

ErgoOne E · User Manual · 9

CORRECT PIPETTING with the ErgoOne®E

1. Attach the tip

Hold the pipette vertically and immerse the tip

2 to 3 mm into the liquid.

Press the pipetting key once

to aspirate the liquid into the

tip. The arrow in the display

points upwards to indicate

the aspiration of liquid.

2. Aspirate liquid

Hold the pipette at an angle between 30º and 45º,

place the tip against the vessel wall.

Press the pipetting key again

and the liquid is completely

discharged including au-

tomatic blow-out. Wipe

pipette tip against the vessel

wall.

3. Discharge liquid After the liquid has been aspirated, the arrow in the

display points downwards to indicate discharging.

4. Eject tip Hold the pipette shaft over

a suitable disposal container

and the press the tip ejec-

tion key. Tip ejection

key

The volume is set at the factory to the nominal volume for the ErgoOne®E and can be

changed quickly and easily. See page 8.

Quick start in the standard pipetting mode

To avoid the intake of air, leave the tip immersed

into the liquid for approx. 1 sec.

Use the correct tip according to the volume range or the color code. Ensure

that the tip is securely seated. When using the flexible pipette shaft, attach an

alternative ejector adjustment clip if necessary.

Pipette tips are disposables items!

ISO 8655 prescribes rinsing the pipette tip once with the sample liquid prior

to the actual pipetting process.

NOTE:

10 · ErgoOne E · User Manual

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