Teledyne Installation Guide Bedienungsanleitung

CombiFlash®Rf

Installation Guide

For indoor use only

Teledyne Isco, Inc.

4700 Superior St., Lincoln, Nebraska, U.S.A. 68504

Phone: (402) 464-0231

Toll Free: (800) 228-4373

FAX: (402) 465-3022

Revision A: August 15, 2007

Part #60-5233-385

iii

CombiFlash®Rf

Installation Guide

Table of Contents

1. Introduction

1.1 Documentation Overview . . . . . . . . . . . . . . . . . . . . . . 1-1

1.2 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

1.3 Operating Overview . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

1.3.1 Multiple Control Possibilities . . . . . . . . . . . . . 1-3

1.3.2 File Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

1.4 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

1.5 Controls, Indicators, and Features . . . . . . . . . . . . . .1-5

1.6 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

1.6.1 Hazard Severity Levels . . . . . . . . . . . . . . . . . .1-9

1.6.2 Hazard Symbols . . . . . . . . . . . . . . . . . . . . . . .1-10

1.7 For Additional Information . . . . . . . . . . . . . . . . . . .1-11

2. System Preparation

2.1 Unpacking the Unit . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

2.2 Instrument Location . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

2.3 Connect Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

2.4 Connect Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . 2-2

2.5 Connect Waste Lines . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

2.6 Optional External Gas . . . . . . . . . . . . . . . . . . . . . . . .2-9

2.7 Connect and Route Drain Lines . . . . . . . . . . . . . . . .2-9

2.8 Position the System . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9

2.9 Install Collection Tube Racks . . . . . . . . . . . . . . . . .2-10

2.10 Turn on Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12

2.11 Configure the System . . . . . . . . . . . . . . . . . . . . . . . . 2-12

2.11.1 Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12

2.11.2 General Settings . . . . . . . . . . . . . . . . . . . . . . . 2-13

2.11.3 Vapor Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13

2.11.4 Automatically Print Report at End of Run . .2-14

2.11.5 Enable Run Length Extension . . . . . . . . . . . . 2-14

2.11.6 Set Default Tube Volumes . . . . . . . . . . . . . . .2-15

2.11.7 Set Date/Time . . . . . . . . . . . . . . . . . . . . . . . . .2-15

2.11.8 Default Run Units . . . . . . . . . . . . . . . . . . . . .2-16

2.11.9 Gradient Method . . . . . . . . . . . . . . . . . . . . . .2-16

2.12 Prime the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . 2-17

CombiFlash® Rf Installation Guide

3. Operation

3.1 Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.1.1 Liquid Sample Injection . . . . . . . . . . . . . . . . . 3-1

3.1.2 Solid Samples . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.1.3 Preloading on Column . . . . . . . . . . . . . . . . . . 3-3

3.2 Loading a RediSep RfColumn . . . . . . . . . . . . . . . . . 3-3

3.3 Start a Default Method . . . . . . . . . . . . . . . . . . . . . . . 3-4

3.4 During the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

3.4.1 Run Control Buttons . . . . . . . . . . . . . . . . . . . . 3-7

4. Remote Interfaces

4.1 Network Configuration . . . . . . . . . . . . . . . . . . . . . . . 4-1

4.1.1 Network PC Access . . . . . . . . . . . . . . . . . . . . . 4-2

4.1.2 Network Printing . . . . . . . . . . . . . . . . . . . . . . 4-3

4.1.3 Automatically Print Report at End of Run . . 4-4

4.2 Direct Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

4.2.1 Windows 2000 Settings . . . . . . . . . . . . . . . . . . 4-5

4.2.2 Windows XP Settings . . . . . . . . . . . . . . . . . . . 4-6

4.2.3 Windows Vista Settings . . . . . . . . . . . . . . . . . 4-8

4.2.4 Completing the Direct Connection . . . . . . . . 4-10

5. Maintenance

5.1 Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . 5-1

5.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

5.2.1 Collection Rack and Tray Cleaning . . . . . . . . 5-2

5.3 Tubing Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

5.4 Flow Cell Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

5.4.1 Post Separation . . . . . . . . . . . . . . . . . . . . . . . . 5-4

5.4.2 Quick Cleaning when Recommended . . . . . . . 5-5

5.4.3 Monthly Flow Cell Cleaning . . . . . . . . . . . . . . 5-9

5.5 Electrical Troubleshooting . . . . . . . . . . . . . . . . . . . 5-10

1-1

CombiFlash®Rf

Installation Guide

Section 1 Introduction

1.1 Documentation Overview

This Installation Guide provides:

• Safety Information

• Unpacking instructions

• Installation instructions, including placing the

instrument on a network and direct connections with a

Windows computer

• Certification and warranty information.

Once you are operating the CombiFlash Rf, you may refer to

the Help menu for operating instructions and further

assistance.

The CD that accompanies this installation guide contains this

guide in Adobe Acrobat PDF format and the PeakTrak®

On-line Help in PDF format.

1.2 Product Overview

The Teledyne Isco CombiFlash Rfflash chromatography

system gives you high-productivity automation,

programmable gradients, full-spectrum UV detection and

peak separation, and automatic detection of columns and

collection tube racks. Its small size makes it a great “personal

system” and well suited for operation within chemical hoods

and other limited spaces.

WARNING

Avoid hazardous practices! If you use this instrument in any

way not specified in this manual, the protection provided by

the instrument may be impaired; this may increase your risk

of injury.

CombiFlash® Rf Installation Guide

1-2

Figure 1-1 CombiFlash Rf

Control of the system is through a simple to use interface. This

interface can be accessed through the touch screen panel, or a

Windows computer via a direct or network Ethernet

connection. These options give you the flexibility to operate

and monitor the CombiFlash Rfsystem at the instrument,

from a computer in the lab, or from remote locations.

The CombiFlash Rfis optimized for use with Teledyne Isco’s

RediSep®Rfcolumns, which are pre-packed with a variety of

media. For example, the CombiFlash Rfcan purify samples

from 4 milligrams to greater than 33 grams using RediSep Rf

columns loaded with 4 to 330 grams of silica gel.

Applications include purification of organic compounds for

drug discovery, as well as research in agrochemicals,

petrochemicals, natural products, polymers, and catalysts.

Section 1 Introduction

1-3

1.3 Operating Overview

The CombiFlash Rfmay be controlled through its built-in

touch screen panel or by a Windows computer running

Microsoft Internet Explorer 6 or newer.

The CombiFlash Rfis equipped with the touch screen display

for local control. For Windows computer control, the

CombiFlash Rfmay be controlled by the computer in two

different ways using TCP/IP protocol—direct or network. A

direct connection uses a cross-over cable between Ethernet

ports on the CombiFlash Rfand computer. A network

connection allows control from a computer on an established

network.

Note

Teledyne Isco recommends that you obtain assistance from your

Information Technology department before attempting direct or

network connections to a Windows computer.

1.3.1 Multiple Control Possibilities

The system can be accessed from the built-in touch panel and

up to ten network computers. The touch panel shares control

with all connected computers. The system performs the most

recent command from any control input.

1.3.2 File Storage

To support operation from a variety of direct and network

connections, the software and all files are stored in the

CombiFlash Rfon an internal hard drive. This ensures that

your compound purification methods and run history files can

be viewed from any connection.

CombiFlash® Rf Installation Guide

1-4

1.4 Specifications

Table 1-1 Specifications 1

Overall Dimensions Height: 61 cm (24")

Width: 36 cm (14.1")

Depth: 43 cm (17")

Weight 24.5 kg (54 lbs)

Power Options Input voltage range from 100 to 240 VAC, 2.0

Amps maximum

Line Frequency 50/60 Hz

Ambient Temperature 20 to 40°C (maximum temperature must be

at least 15°C below the boiling point of the

solvent)

Humidity (when

connected to power)

95% relative humidity maximum at 20 to

40°C

Flow Rate Range 5 to 100 ml/min

Flow Rate Accuracy ±5%

Pressure Limit Up to 200 psi (column dependant)

For columns undetected by RFID, the

maximum pressure is limited to 50 psi.

Pressure Accuracy 5% of full scale

Peak Detection Modes Slope or threshold

UV Spectrum 200 to 360 nm

Wavelength Accuracy ±5 nm

Flow Cell Pathlength 0.1 mm, ±25%

Fraction Accuracy ±[2ml +(flow rate ÷60)]

Pollution Degree 2

Electrical Safety per EN 61010-1

Installation Category II

Maximum Altitude 2000 meters

Note 1. All specifications are preliminary and subject to change.

Section 1 Introduction

1-5

1.5 Controls, Indicators, and Features

Figures 1-2 through 1-4 illustrate the controls on the

CombiFlash Rf.

Figure 1-2 CombiFlash RfFeatures (Front)

1. Touch Panel LCD display – Large 10.5 inch display for

system monitoring and control.

2. USB Port – Convenient, front panel port that accepts USB

Flash memory drive. A Flash memory drive may be

inserted into this port for transferring files, importing and

exporting methods, and system software updates.

3. Adjustable Column Mount – The injection valve

assembly and upper column mount slide along this mount

so the system can accept a variety of column sizes.

4. On/Standby Switch – 1 = On, 0 = standby.

5. Fraction Collector Arm and Drop Former – The arm

and drop former move to deposit liquid in the collection

tubes.

CombiFlash® Rf Installation Guide

1-6

6. Racksand Collection Tubes – The racks hold the

fraction collection tubes. Racks include an RFID tag which

the system uses to read the rack type and collection tube

size.

7. Sample Injection Port – Luer-type fitting to accept the

sample though either a solid load sample cartridge (shown)

or a liquid injection using a syringe or similar device.

8. Injection Valve – This six-way valve is automatically

positioned by the CombiFlash Rfaccording to its current

mode of operation. Modes include column equilibration,

sample injection, elution, column flushing, valve cleaning,

and system purging.

9. Upper Column Mount – Secures the column inlet.

10.Lower Column Mount – Secures the column outlet.

11.Top Shelf – Allows storage of accessories and solvent

bottles.

12.Column Expansion Ports – These ports allow the system

to add column channels with an optional module. This

option is not available at the time of printing.

13.Upper Drain Tube – Liquids spilled on the top shelf are

carried away through this tube to a user-supplied

container.

14.Ethernet Port – An 8P8C jack for a network connection

using a standard CAT5 cable, or for a direct connection to a

computer using a cross-over cable.

15.RS-232 Port – Serial communication port to interface with

expansion modules.

16.USB Port – For control of peripheral equipment. This rear

panel port is primarily for service-related functions.

17.Cooling Fan – Cools the internal electronic assemblies.

18.Check Valves – Externally-mounted pump check valves to

simplify pump maintenance.

19.Solvent Level Sensing Outlet Ports – uses air or

user-supplied gas to measure the hydrostatic pressure of

the solvent above the weighted tubing outlet.

20.Solvent Inlet Ports – Accepts two A solvents and two B

solvents.

Section 1 Introduction

1-7

Figure 1-3 CombiFlash RfFeatures (Side)

Figure 1-4 CombiFlash RfFeatures (Back)

19 20

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