Thermo Scientific Microm HM355S Bedienungsanleitung

Thermo Fisher Scientiﬁc Operation Manual HM335S 1

Part of Thermo Fisher Scientiﬁc

Microm HM355S

Rotary Microtome

Operation Manual – English / German

ex Ser. No 44478

Thermo Fisher Scientiﬁc

Operation Manual HM335S

Thermo Fisher Scientiﬁc Operation Manual HM335S 3

Microm HM355S

Rotary Microtome

Operation Manual – English

ex Ser. No 44478

Thermo Fisher Scientiﬁc

Operation Manual HM335S

ermo Fisher Scientic Microm International GmbH is an ISO 9001 Company.

ermo Scientic is the trading name of ermo Fisher Scientic Microm International GmbH.

All other trademarks are the property of ermo Fisher Scientic and its subsidiaries. ermo Fisher

Scientic makes every attempt to ensure that the information contained in this support documentation is

correct and clearly stated but does not accept responsibility for any errors or omissions. e development of

ermo Scientic products and services is an ongoing process. Please ensure that any published information

you use as a reference is up to date and relates to the condition of the product. If necessary, check with ermo

Fisher Scientic or your local ermo Fisher Scientic representative.

is Operation Manual may not, in whole or in part, be copied, photocopied, reproduced, translated or con-

verted to any electronic or other form without prior written consent of ermo Fisher Scientic.

All information contained in this manual is proprietary and condential, and the exclusive property of ermo

Fisher Scientic. is manual is protected by copyright and any reproduction is prohibited. It is only be used

by the individuals to whom it has been made available through ermo Fisher Scientic.

Contact addresses Anatomical Pathology

Microm International GmbH

part of ermo Fisher Scientic

Otto-Hahn-Strasse 1a

69190 Walldorf

Germany

Tel: +49 (0) 6227 836-0

Fax: +49 (0) 6227 836-111

www.ap-walldorf.com

info.dxd.dewal@thermosher.com

4481 Campus Drive

Kalamazoo

MI 49008, USA

Tel: 1-800-522-7270

Fax: +1 269-372-2674

www.thermo.com/pathology

e ermo Scientic Microm HM355S meets the following CE Mark requirements:

In Vitro Diagnostic Directive 98/79/EC

11/11

Preface

Thermo Fisher Scientiﬁc Operation Manual HM335S 05

Dear valued customer,

ank you for buying a ermo Scientic instrument.

Before using the instrument you purchased, please read these operating

instructions carefully to familiarize yourself with the proper operation

and functions. To avoid risks to the operator and technical damage to

the instrument, it must only be used in accordance with its intended

use (page 11). Any other use of the instrument is considered improper!

All warranties and guarantees would then be null and void.

is Operation Manual was specially written for the routine user work-

ing with the Rotary Microtome HM355S. e chapters contain useful

and important information and should be considered during the daily

routine work.

Only skilled or specially trained personnel should operate the micro-

tome and perform such tasks as clamping the specimens, trimming and

rst-cuts, sectioning and transferring sections onto a slide. e safety

instructions given in this manual, the regular safety standards as well as

the regulations and hygiene measures of your respective lab should be

strictly observed.

Please keep this Operation Manual near to the instrument for later

consultation.

is Operation Manual will be supplied together with each instrument.

Additional copies can be ordered at the nearest ermo Fisher Scientic

sales oce by giving the serial number of the instrument (placed on the

rear panel) the part number of the instruction manual and the number

of version.

• English/German (printed version): 387861

• CD-Rom (including English, German, Italian, Spanish, French):

387862

Welcome

Preface

Thermo Fisher Scientiﬁc

Operation Manual HM335S

Contents

Important Information and Safety

Symbols 08

Personal Safety 08

General Safety 10

Intended Use 11

Introduction 12

Description Rotary Microtome Microm HM355S 12

Scope of Delivery 13

Additional Equipment (optional) 14

Technical Data Sheet 16

Operating Instructions 17

Setting up the Microtome 17

Initial Start-up 20

Using the Food Pedal (optional) 21

Operating Panel 22

Display and Key Functions 23

Setting Cutting Window 23

Starting and Stopping of the Cutting Drive 23

Hand Wheel Brakes 24

Emergency Stop 25

Cutting Process Indicators 26

Section Counter 26

Section ickness Sum 27

Remaining Distance to Front End Position 27

Setting Section and Trimming ickness 28

Trimming and First Cuts 29

Fine Feed 30

Cutting Movement and Retraction 30

Motorized Cutting Drive 32

Specimen Coarse Feed 32

Operating Modes 33

Selection of Operating Modes 33

Time and Date 36

Display Mode 36

Turning o the “Retraction” Function 37

Language Selection for the Display 38

Setting Cutting Speed 39

Factory Defaults 40

Memory Function 40

Rocking Mode Function 41

Push-in Hand Wheel Handle 42

Adapters for Specimen Clamping 43

Adapter, non-orienting 43

Adapter, orienting, Specimen Orientation 43

Chapter 1

Chapter 2

Chapter 3

Contents

Thermo Fisher Scientiﬁc Operation Manual HM335S 07

Changing and/or Fastening Specimen Clamps 44

Readjusting Specimen Clamps 45

Specimen Clamping 46

Universal Cassette Clamp 46

Universal Cassette Clamp, adjustable 46

Standard Specimen Clamp 47

Insert for Round Specimens, V-Insert and V-Distance piece 48

Foil Clamp 49

Segment Arc and Universal Specimen Holder 50

Knife and Blade Carriers 50

Disposable Blade Carrier “ER” 51

Disposable Blade Carrier “E” 53

Knife Carrier “C” 55

Re-adjusting Knife and Blade Carriers 57

Section Waste Tray with Integrated Arm Rest 58

Large Field Magnier 58

Working with the Microtome 60

Sectioning Instructions 60

How to Avoid Errors 63

Possible Sources of Errors – Cause and Removal 64

Maintenance and Care 65

Cleaning and Care 65

Maintenance 66

Replacement Work 67

Conditions for Transportation 68

Returning the Instrument for Repair or Routine Maintenance 68

Disposal of the Instrument after Final Shutdown 69

Warranty Statement and Certiﬁcation 70

Chapter 4

Chapter 5

Chapter 6

Chapter 7

Contents

Thermo Fisher Scientiﬁc

Operation Manual HM335S

Chapter 1

Important Information

e following symbols and conventions are used throughout this

manual and on the instrument.

WARNING

CAUTION

S       

   . F       -

   ,     .

Note:

Special Instructions regarding operation of the instrument

azard of hand injuries

         

     .

CAUTION

– general danger spot

        

    .     

   .

Biohazard

   .

Waste disposal

       

     :     -

         

      .  

        .

     .  

  .

instrunctions for using microtome knife

           ,

         

     .  ,  

           

.     ,   

           .

        .   

Symbols

Personal Safety

Important Informations

Symbols

Thermo Fisher Scientiﬁc Operation Manual HM335S 09

            -

    . T     

hazard of Biological danger

S        

   .   ,   -

       

  .   

,  ,      

       :   

  .    

     .  -

   ,    -

     .    

    ,   (..

 , ,   )   .

        .

sticker

“stop“

A    -      

             -

. A      

        

. A       .

D     .     

  .

emergency stop

        

     .    

        .     

  .       

    ().     

   . T       

      .

sticker

“

Brake“ With arroW

A      “”     

  -         -

. T           

 . H        -

        –    

    .

Important Informations

Safety

STOP

Thermo Fisher Scientiﬁc

Operation Manual HM335S

caution!

        

.       

.       

        . 

          

      .

grounding the instrument

     ,   

       . T

       .  

          

    (IEC) -

.   ,        

  .         

    .

caution: high voltage

     . 

         

  .      

  .

caution – notify a service technician!

         

 .      

    .

danger in flammaBle environment

          -

 .

hazard of electromagnectic interference

        ,

       .

          

.      /  ,

         .

hazard of radiation

       

  .    

 ,    

    .      

    ,   -

  ,   (.. 

, ,   )   . 

        

 .

Important Informations

Safety

General Safety

Andere Handbücher für Microm HM355S

Inhaltsverzeichnis

Andere Thermo Scientific Laborausrüstung Handbücher

Thermo Scientific

Thermo Scientific Finnpipette Novus Bedienungsanleitung

Thermo Scientific

Thermo Scientific TSQ 8000 Evo Bedienungsanleitung

Thermo Scientific

Thermo Scientific Orion Versa Star Bedienungsanleitung

Thermo Scientific

Thermo Scientific Ion Trap Series Bedienungsanleitung

Thermo Scientific

Thermo Scientific Medifuge Bedienungsanleitung

Thermo Scientific

Thermo Scientific DensityPRO-T Bedienungsanleitung

Thermo Scientific

Thermo Scientific Sorvall Legend X1R Bedienungsanleitung

Thermo Scientific

Thermo Scientific 88881-003 Bedienungsanleitung

Thermo Scientific

Thermo Scientific NanoDrop One Bedienungsanleitung

Thermo Scientific

Thermo Scientific Sorvall LYNX 4000 Bedienungsanleitung

Thermo Scientific

Thermo Scientific Vanquish Charger Bedienungsanleitung

Thermo Scientific

Thermo Scientific Shandon TissueWave 2 Handbuch

Thermo Scientific

Thermo Scientific TEOM 1405 Bedienungsanleitung

Thermo Scientific

Thermo Scientific Orion 2111XP Bedienungsanleitung

Thermo Scientific

Thermo Scientific Applied Biosystems GeneChip 645 Bedienungsanleitung

Thermo Scientific

Thermo Scientific Precision U01366 Bedienungsanleitung

Thermo Scientific

Thermo Scientific Nicolet iS50 GC-IR Bedienungsanleitung

Thermo Scientific

Thermo Scientific BF51842C Bedienungsanleitung

Thermo Scientific

Thermo Scientific 700 Series Betriebsanleitung

Thermo Scientific

Thermo Scientific Sorvall ST40R Bedienungsanleitung

Thermo Scientific

Thermo Scientific Finnpipette Bedienungsanleitung

Thermo Scientific

Thermo Scientific Thermo Scientific 3906 Bedienungsanleitung

Thermo Scientific

Thermo Scientific Forma 8602 Betriebsanleitung

Thermo Scientific

Thermo Scientific TSQ Altis Bedienungsanleitung

Beliebte Laborausrüstung Handbücher anderer Marken

Qiagen

Qiagen DML 3000 Bedienungsanleitung

Queensgate

Queensgate NANOSCAN OP400 Programmierhandbuch

Conviron

Conviron GEN1000-GE Bedienungsanleitung

Parr Instrument

Parr Instrument 4560 Bedienungsanleitung

IKA

IKA IC control Bedienungsanleitung

Integra

Integra DOSE IT Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5800 ICP-OES Bedienungsanleitung

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Bedienungsanleitung

NI PXI-5422 Handbuch

Collomix

Collomix Aqix Bedienungsanleitung

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Bedienungsanleitung

Ocean Insight

Ocean Insight FLAME-NIR+ Bedienungsanleitung

Parker

Parker ALIGN-MG-NA Bedienungsanleitung

BD 644787 Bedienungsanleitung

DENTAURUM

DENTAURUM Compact Megaplus Bedienungsanleitung

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Bedienungsanleitung

VWR

VWR SAS Super IAQ Bedienungsanleitung

illumina

illumina MiSeqDx Bedienungsanleitung