THORLABS OTM211 Bedienungsanleitung
OTM211
Optical Tweezer System
User Guide
Table of Contents
Chapter 1Warning Symbol Definitions..............................................................................................3
Chapter 2Safety....................................................................................................................................4
Chapter 3Description...........................................................................................................................5
3.1.Introduction .....................................................................................................................5
3.2.Theory ..............................................................................................................................5
3.3.Key System Features......................................................................................................6
3.4.System Components.......................................................................................................7
3.5.Optics Module..................................................................................................................8
3.6.Power Supply and Electronic Module ...........................................................................9
3.7.Interlock System..............................................................................................................9
Chapter 4Operation.............................................................................................................................10
4.1.Starting the System.......................................................................................................10
4.2.Graphical User Interface...............................................................................................10
4.3.SDK.................................................................................................................................11
Chapter 5Operating Conditions and Environmental Requirements............................................12
5.1.Selecting a Suitable Operation Environment .............................................................12
Chapter 6System Connections and Care.........................................................................................13
6.1.Module Connections.....................................................................................................13
6.1.1.Cable Descriptions .............................................................................................................. 13
6.2.Service............................................................................................................................14
6.3.Accessories and Customization..................................................................................14
Chapter 7Specifications.....................................................................................................................15
7.1.Laser Specifications .....................................................................................................15
7.2.Power Requirements.....................................................................................................15
7.3.Dimensions and Weights..............................................................................................15
Chapter 8Regulatory...........................................................................................................................16
8.1.Waste Treatment is Your Own Responsibility............................................................16
8.2.Ecological Background ................................................................................................16
Chapter 9Thorlabs Worldwide Contacts..........................................................................................17
OTM211 Chapter 1: Warning Symbol Definitions
Rev A, October 7, 2015 Page 3
Chapter 1 Warning Symbol Definitions
Below is a list of warning symbols you may encounter in this manual or on your device.
Symbol Description
Direct Current
Alternating Current
Both Direct and Alternating Current
Earth Ground Terminal
Protective Conductor Terminal
Frame or Chassis Terminal
Equipotentiality
On (Supply)
Off (Supply)
In Position of a Bi-Stable Push Control
Out Position of a Bi-Stable Push Control
Caution: Risk of Electric Shock
Caution: Hot Surface
Caution: Risk of Danger
Warning: Laser Radiation
Caution: Spinning Blades May Cause Harm
OTM211 Chapter 2: Safety
Rev A, October 7, 2015 Page 4
Chapter 2 Safety
All statements regarding safety of operation and technical data in this instruction manual will only
apply when the unit is operated correctly.
SHOCK WARNING
Before applying power to the system, make sure that the protective conductor of the three-
conductor mains power cord is correctly connected to the protective earth contact of the socket
outlet. Improper grounding can cause electrical shock resulting in serious personal injury or death.
Ensure that the line voltage switch is set correctly for your local supply and that the appropriate
fuses are installed. Fuses should only be changed by qualified service personnel.
The OTM211 contains no user-serviceable parts. Do not operate without cover installed.
Refer all maintenance to suitably qualified personnel.
Contact Thorlabs for assistance.
CAUTION
Do not obstruct the unit’s air ventilation slots and fan outlets.
WARNING
This equipment contains a high power infrared laser. Radiations from this laser are not visible to the
human eye and can cause serious damage to your eyesight.
All laser radiation is enclosed during normal operation.
Do not attempt to defeat interlocks.
!
!
LASER PRODUCT
CLASS 1
OTM211 Chapter 3: Description
Rev A, October 7, 2015 Page 5
Chapter 3 Description
3.1. Introduction
The Thorlabs OTM211 is an optical tweezers system designed for inverted microscopes. It provides two
independent trapping beams that allow the manipulation of nano- and micro particles. Each path can be used to
create several additional traps. The system is completely computer controlled and offers a graphical user interface
which provides the user with a set of tools for the most common trapping experiments. The detector module included
in the OTM211 allows users to perform quantitative force measurements and particle tracking. The included
Software Development Kit enables users to easily create applications optimized for their specific needs and allows
integration into more complex setups.
The OTM211 system integrates with existing imaging modalities, such as fluorescence, Raman, confocal, phase
contrast. Further on the system is compatible with all major microscope platforms.
3.2. Theory
Optical Tweezers, or traps as they are often called, are created by using a high numerical aperture objective to
tightly focus a laser beam thereby creating a spot where a dielectric particle with dimensions of the order of 0.3µm
to a few microns will experience a force due to transfer of momentum from the scattering of photons.
Arthur Ashkin in the early 1970s originally demonstrated that optical forces can manipulate micron-sized dielectric
particles in water (
Ashkin et al, Optics Letters, Vol 11, No. 5, may 1986
). This technique has become an
important tool in a wide range of fields such as bioengineering, material science, and physics due to their ability to
hold and manipulate micron-sized particles and to measure forces in the piconewton and femtonewton ranges.
Figure 1 Theory of Optical Trapping
The capability of optical tweezers to exert measurable forces on dielectric particles offers a unique and valuable
tool for studying cell components such as biological proteins and molecular motors. In many investigations, optical
tweezers apply force to functionalized microspheres that have been attached to molecules of interest. Optical
tweezers apply a force toward the focus of the trapping laser beam with a magnitude proportional to the distance
of the particle from the focus, for small displacements from the center of the trap. This allows the optical tweezers
to be modeled by Hooke’s law, F= -kd, where d is the displacement from the center of the trap and k is the force
constant. As the laser beam passes through a trapped particle, it will be deflected by an amount that depends on
the position of the particle. Accurate force measurements depend on precise calibration of the spring constant, k,
and the particle position which varies with laser power and particle properties.
Three methods for ascertaining the force constant, k are: Equipartition, PSD Rolloff and Stokes’ drag. Since each
method relies on a different physical principle, the combined results provide a convenient way to verify the
calibration.
OTM211 Chapter 3: Description
Rev A, October 7, 2015 Page 6
The Equipartation method equates the average potential energy of the particle in the trap to the thermal energy of
the solvent molecules. In this method, a sample of particle positions is obtained by recording the position detector
output over time and converting the voltages to distances using the detector responsivity. For PSD roll off, the power
spectral density of a time series of trapped particle positions is computed and fitted to the response of a harmonic
oscillator with known damping due to the viscosity of the solvent. In the Stokes method, the sample is translated in
a range of velocities. A force balance between viscous drag on the particle and the trap force is computed.
3.3. Key System Features
Multiple traps with computer control
Force Measurement Capability
Software user interface with enhanced mouse control of multiple time shared traps
Multiple trap pattern creation with arbitrary spacing – circles, lines, rectangles
IR laser with independent power control
Two independent trap paths with multiple time-shared traps per path
Active intensity stabilization of each trap beam with integrated noise eaters
Concurrent standard microscopy techniques like fluorescence
Compatible with major inverted microscope platforms
Fully enclosed beam path – Class 1 certified
Galvo-scanning beam control
SDK package
OTM211 Chapter 3: Description
Rev A, October 7, 2015 Page 7
3.4. System Components
The OTM211 includes the following modules:
Optics Module
1.3NA Trapping Objective and Condenser
1600 x 1200 CCD Camera with Imaging Adapter
Electronics Control Module
Power Supply Module
Nikon Eclipse Ti-S Inverted Microscope with high resolution XYZ Piezo Stage
Force Module
Computer with Monitor and Accessories
Figure 2 Modules of Complete OTM211 System
Optics Module
Electronics
Control Module
Force Module
Safety Interlocks
Microscope
Stage Controller
Power Supply
Module
OTM211 Chapter 3: Description
Rev A, October 7, 2015 Page 8
3.5. Optics Module
The optics module includes all the optical and opto-electronic components for creating and controlling multiple
optical traps. The conditioned output beam is coupled into the microscope. A schematic for this module is shown
below.
Figure 3 Schematic of Optics Module
OTM211 Chapter 3: Description
Rev A, October 7, 2015 Page 9
3.6. Power Supply and Electronic Module
These two modules provide power and contain the control circuitry for the components in the optics module. The
rear panel of the power supply module has connection to the mains power supply and to the electronics module.
The front panel has an LED indicator when power is ON.
Figure 4 Power Supply Module Front Panel
The Electronics module houses the laser source . The rear panel has the interfaces for all cable connections to the
optics and power supply modules. The bottom right of the front panel of the electronics control module has a key
and an emergency shut-off switch for the laser. Three LEDs at the top are used to indicate main power, PC
connection, and USB data transfer.
Figure 5 Electronic Module Front Panel
3.7. Interlock System
For user safety, the system has four interlocks. There is the illumination pillar interlock, the viewport interlock,
sample cover interlock. Additionally a remote interlock connection is provided for user integration. All four interlocks
must be closed for laser emission.
The system is classified as a Class 1 laser.
Figure 6 Interlock System: (left) Viewport (and (right) Sample Cover
Laser Key and
indicator
Emergency
shutoff
OTM211 Chapter 4: Operation
Rev A, October 7, 2015 Page 10
Chapter 4 Operation
4.1. Starting the System
To power up the OTM211 system, follow the steps below.
1. Start the Power Supply Module by placing the POWER switch on the back of the unit to the “I” position.
Verify that the “POWER ON” indicator turns green on the lower front side of the unit.
2. At this time, the “Power” and “Ready” indicators on the Electronics Control Module will turn on.
3. Turn the key switch on the Electronics Module to the “ON” position.
4. Check the emergency stop button and make sure it is in the “Enable” position.
5. Make sure all interlocks are closed.
4.2. Graphical User Interface
The OTM211 system comes with a pre-installed software which contains everything needed for system control and
data acquisition. In addition an SDK is provided to allow users to easily create applications optimized for their
particular requirements.
The GUI has five main areas:
The Camera Display
The Laser Control
The Stage Control
The Trap Control
Tools
Figure 7 OTM211 Software User Interface
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