THORLABS OTM211 Bedienungsanleitung

OTM211

Optical Tweezer System

User Guide

Table of Contents

Chapter 1Warning Symbol Definitions..............................................................................................3

Chapter 2Safety....................................................................................................................................4

Chapter 3Description...........................................................................................................................5

3.1.Introduction .....................................................................................................................5

3.2.Theory ..............................................................................................................................5

3.3.Key System Features......................................................................................................6

3.4.System Components.......................................................................................................7

3.5.Optics Module..................................................................................................................8

3.6.Power Supply and Electronic Module ...........................................................................9

3.7.Interlock System..............................................................................................................9

Chapter 4Operation.............................................................................................................................10

4.1.Starting the System.......................................................................................................10

4.2.Graphical User Interface...............................................................................................10

4.3.SDK.................................................................................................................................11

Chapter 5Operating Conditions and Environmental Requirements............................................12

5.1.Selecting a Suitable Operation Environment .............................................................12

Chapter 6System Connections and Care.........................................................................................13

6.1.Module Connections.....................................................................................................13

6.1.1.Cable Descriptions .............................................................................................................. 13

6.2.Service............................................................................................................................14

6.3.Accessories and Customization..................................................................................14

Chapter 7Specifications.....................................................................................................................15

7.1.Laser Specifications .....................................................................................................15

7.2.Power Requirements.....................................................................................................15

7.3.Dimensions and Weights..............................................................................................15

Chapter 8Regulatory...........................................................................................................................16

8.1.Waste Treatment is Your Own Responsibility............................................................16

8.2.Ecological Background ................................................................................................16

Chapter 9Thorlabs Worldwide Contacts..........................................................................................17

OTM211 Chapter 1: Warning Symbol Definitions

Rev A, October 7, 2015 Page 3

Chapter 1 Warning Symbol Definitions

Below is a list of warning symbols you may encounter in this manual or on your device.

Symbol Description

Direct Current

Alternating Current

Both Direct and Alternating Current

Earth Ground Terminal

Protective Conductor Terminal

Frame or Chassis Terminal

Equipotentiality

On (Supply)

Off (Supply)

In Position of a Bi-Stable Push Control

Out Position of a Bi-Stable Push Control

Caution: Risk of Electric Shock

Caution: Hot Surface

Caution: Risk of Danger

Warning: Laser Radiation

Caution: Spinning Blades May Cause Harm

OTM211 Chapter 2: Safety

Rev A, October 7, 2015 Page 4

Chapter 2 Safety

All statements regarding safety of operation and technical data in this instruction manual will only

apply when the unit is operated correctly.

SHOCK WARNING

Before applying power to the system, make sure that the protective conductor of the three-

conductor mains power cord is correctly connected to the protective earth contact of the socket

outlet. Improper grounding can cause electrical shock resulting in serious personal injury or death.

Ensure that the line voltage switch is set correctly for your local supply and that the appropriate

fuses are installed. Fuses should only be changed by qualified service personnel.

The OTM211 contains no user-serviceable parts. Do not operate without cover installed.

Refer all maintenance to suitably qualified personnel.

Contact Thorlabs for assistance.

CAUTION

Do not obstruct the unit’s air ventilation slots and fan outlets.

WARNING

This equipment contains a high power infrared laser. Radiations from this laser are not visible to the

human eye and can cause serious damage to your eyesight.

All laser radiation is enclosed during normal operation.

Do not attempt to defeat interlocks.

LASER PRODUCT

CLASS 1

OTM211 Chapter 3: Description

Rev A, October 7, 2015 Page 5

Chapter 3 Description

3.1. Introduction

The Thorlabs OTM211 is an optical tweezers system designed for inverted microscopes. It provides two

independent trapping beams that allow the manipulation of nano- and micro particles. Each path can be used to

create several additional traps. The system is completely computer controlled and offers a graphical user interface

which provides the user with a set of tools for the most common trapping experiments. The detector module included

in the OTM211 allows users to perform quantitative force measurements and particle tracking. The included

Software Development Kit enables users to easily create applications optimized for their specific needs and allows

integration into more complex setups.

The OTM211 system integrates with existing imaging modalities, such as fluorescence, Raman, confocal, phase

contrast. Further on the system is compatible with all major microscope platforms.

3.2. Theory

Optical Tweezers, or traps as they are often called, are created by using a high numerical aperture objective to

tightly focus a laser beam thereby creating a spot where a dielectric particle with dimensions of the order of 0.3µm

to a few microns will experience a force due to transfer of momentum from the scattering of photons.

Arthur Ashkin in the early 1970s originally demonstrated that optical forces can manipulate micron-sized dielectric

particles in water (

Ashkin et al, Optics Letters, Vol 11, No. 5, may 1986

). This technique has become an

important tool in a wide range of fields such as bioengineering, material science, and physics due to their ability to

hold and manipulate micron-sized particles and to measure forces in the piconewton and femtonewton ranges.

Figure 1 Theory of Optical Trapping

The capability of optical tweezers to exert measurable forces on dielectric particles offers a unique and valuable

tool for studying cell components such as biological proteins and molecular motors. In many investigations, optical

tweezers apply force to functionalized microspheres that have been attached to molecules of interest. Optical

tweezers apply a force toward the focus of the trapping laser beam with a magnitude proportional to the distance

of the particle from the focus, for small displacements from the center of the trap. This allows the optical tweezers

to be modeled by Hooke’s law, F= -kd, where d is the displacement from the center of the trap and k is the force

constant. As the laser beam passes through a trapped particle, it will be deflected by an amount that depends on

the position of the particle. Accurate force measurements depend on precise calibration of the spring constant, k,

and the particle position which varies with laser power and particle properties.

Three methods for ascertaining the force constant, k are: Equipartition, PSD Rolloff and Stokes’ drag. Since each

method relies on a different physical principle, the combined results provide a convenient way to verify the

calibration.

OTM211 Chapter 3: Description

Rev A, October 7, 2015 Page 6

The Equipartation method equates the average potential energy of the particle in the trap to the thermal energy of

the solvent molecules. In this method, a sample of particle positions is obtained by recording the position detector

output over time and converting the voltages to distances using the detector responsivity. For PSD roll off, the power

spectral density of a time series of trapped particle positions is computed and fitted to the response of a harmonic

oscillator with known damping due to the viscosity of the solvent. In the Stokes method, the sample is translated in

a range of velocities. A force balance between viscous drag on the particle and the trap force is computed.

3.3. Key System Features

 Multiple traps with computer control

 Force Measurement Capability

 Software user interface with enhanced mouse control of multiple time shared traps

 Multiple trap pattern creation with arbitrary spacing – circles, lines, rectangles

 IR laser with independent power control

 Two independent trap paths with multiple time-shared traps per path

 Active intensity stabilization of each trap beam with integrated noise eaters

 Concurrent standard microscopy techniques like fluorescence

 Compatible with major inverted microscope platforms

 Fully enclosed beam path – Class 1 certified

 Galvo-scanning beam control

 SDK package

OTM211 Chapter 3: Description

Rev A, October 7, 2015 Page 7

3.4. System Components

The OTM211 includes the following modules:

 Optics Module

 1.3NA Trapping Objective and Condenser

 1600 x 1200 CCD Camera with Imaging Adapter

 Electronics Control Module

 Power Supply Module

 Nikon Eclipse Ti-S Inverted Microscope with high resolution XYZ Piezo Stage

 Force Module

 Computer with Monitor and Accessories

Figure 2 Modules of Complete OTM211 System

Optics Module

Electronics

Control Module

Force Module

Safety Interlocks

Microscope

Stage Controller

Power Supply

Module

OTM211 Chapter 3: Description

Rev A, October 7, 2015 Page 8

3.5. Optics Module

The optics module includes all the optical and opto-electronic components for creating and controlling multiple

optical traps. The conditioned output beam is coupled into the microscope. A schematic for this module is shown

below.

Figure 3 Schematic of Optics Module

OTM211 Chapter 3: Description

Rev A, October 7, 2015 Page 9

3.6. Power Supply and Electronic Module

These two modules provide power and contain the control circuitry for the components in the optics module. The

rear panel of the power supply module has connection to the mains power supply and to the electronics module.

The front panel has an LED indicator when power is ON.

Figure 4 Power Supply Module Front Panel

The Electronics module houses the laser source . The rear panel has the interfaces for all cable connections to the

optics and power supply modules. The bottom right of the front panel of the electronics control module has a key

and an emergency shut-off switch for the laser. Three LEDs at the top are used to indicate main power, PC

connection, and USB data transfer.

Figure 5 Electronic Module Front Panel

3.7. Interlock System

For user safety, the system has four interlocks. There is the illumination pillar interlock, the viewport interlock,

sample cover interlock. Additionally a remote interlock connection is provided for user integration. All four interlocks

must be closed for laser emission.

The system is classified as a Class 1 laser.

Figure 6 Interlock System: (left) Viewport (and (right) Sample Cover

Laser Key and

indicator

Emergency

shutoff

OTM211 Chapter 4: Operation

Rev A, October 7, 2015 Page 10

Chapter 4 Operation

4.1. Starting the System

To power up the OTM211 system, follow the steps below.

1. Start the Power Supply Module by placing the POWER switch on the back of the unit to the “I” position.

Verify that the “POWER ON” indicator turns green on the lower front side of the unit.

2. At this time, the “Power” and “Ready” indicators on the Electronics Control Module will turn on.

3. Turn the key switch on the Electronics Module to the “ON” position.

4. Check the emergency stop button and make sure it is in the “Enable” position.

5. Make sure all interlocks are closed.

4.2. Graphical User Interface

The OTM211 system comes with a pre-installed software which contains everything needed for system control and

data acquisition. In addition an SDK is provided to allow users to easily create applications optimized for their

particular requirements.

The GUI has five main areas:

 The Camera Display

 The Laser Control

 The Stage Control

 The Trap Control

 Tools

Figure 7 OTM211 Software User Interface

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