THORLABS RSB1 Bedienungsanleitung

Raman Spectroscopy

Bundle

RSB1(/M,) RSBR1(/M),

RSBC1(/M), RSBA1

Operation Manual

Version:

Date: 1.0

17-Sep-2021

Foreword Table of Contents

1 General Information 1

21.1 Ordering Codes and Accessories 41.2 Requirements

2 Getting Started 5

52.1 Parts List

3 Operating Elements 7

73.1 RSB1(/M) Base Unit 83.2 RSBR1(/M) Front End for Reflective Measurements 93.3 RSBC1(/M) Front End for Cuvette Measurements 103.4 RSBA1 Mounting Brackets for Cage System

4 System Setup 11

114.1 Mounting RSB1(/M) 134.2 Mounting the Front End 134.2.1 Mounting RSBR1(/M) 174.2.2 Mounting RSBC1(/M) 194.2.3 Mounting Cage Systems 204.2.4 Mounting on the SM1.5 External Thread of RSB1(/M) 204.3 Laser and Laser Driver 214.3.1 Laser 214.3.1.1 Alternative Excitation Wavelength 214.3.2 Optical Fiber 214.3.3 Laser Driver 224.3.4 Mounting the Laser 234.4 Connecting the RSB1(/M) to the PC

5 Operating Instructions 24

245.1 Laser Driver Settings 255.2 Software and Installation 275.3 Raman Measurements with the OSA Raman Software 275.3.1 Software Startup Screen 285.3.2 Software Configuration Procedure 295.3.3 Settings 315.3.3.1 Info Panel 325.3.3.2 Main Panel 375.3.3.3 Dark Image 375.3.4 System Calibration 445.3.5 Export Data 455.4 Experimental Measurements

4.1 Polystyrene System Test 465.4.2 Sample Measurement 475.4.3 Compatible Cuvettes for RSBC1(/M) 
Operating Principle 48
1 Analysis of Raman Spectra 506.2 Acquisition of Raman Spectra 526.3 Coded-Aperture Raman Spectroscopy 546.4 Parameters Affecting Spectra 
Maintenance and Service 58
1 Troubleshooting 
Appendix 62
1 Technical Data 638.1.1 RSB1/M and RSB1 Technical Data 648.1.1.1 RSB1/M and RSB1 Dimensions 668.1.2 RSBR1/M and RSBR1 Technical Data 678.1.2.1 RSBR1/M and RSBR1 Dimensions 698.1.3 RSBC1/M and RSBC1 Technical Data 708.1.3.1 RSBC1/M and RSBC1 Dimensions 728.1.4 RSBA1 Technical Data 728.1.4.1 RSBA1 Dimensions 738.1.5 Thorlabs Raman Bundle Technical Data 738.1.6 Laser Requirements 748.1.7 Technical Data Explained 748.1.7.1 Signal-to-Noise Characterization 758.1.7.2 Spectral Accuracy and Spectral Resolution 788.1.7.3 Amplitude Correction 808.2 List of Acronyms 818.3 References 828.4 Application Note Polarization Dependent Raman Spectroscopy 848.4.1 Optimizing Raman Signal via Collimator Rotation 858.4.2 Including Polarization Optics in the Excitation Path 878.4.3 Including Polarization Optics in the Excitation and Raman Scattering Path 888.4.4 References 898.5 OSA Raman Software: General Instructions 898.5.1 Interface Overview 908.5.2 Customizing the Display 908.5.2.1 Zooming and Scrolling the Horizontal Axis 918.5.2.2 Zooming and Scrolling the Vertical Axis 918.5.2.3 Setting the Unit of the Horizontal Axis 918.5.2.4 Setting the Unit of the Vertical Axis 928.5.2.5 Displaying the Secondary Vertical Axis

938.5.2.6 Hiding the Menus and Full Screen Mode 938.5.2.7 Displaying a Split View 948.5.2.8 Displaying a Legend in the Data Display Area 958.5.2.9 Dark Room Mode 968.5.3 Acquiring Data 968.5.3.1 Finding the Spectrometer 968.5.3.2 Selecting the Instrument to Use 978.5.3.3 Acquiring a Single Spectrum 988.5.3.4 Repeated Measurements 988.5.3.5 Averaging Spectra 988.5.4 Movable Markers 998.5.4.1 Moving Markers 998.5.4.2 Marker Values 998.5.4.3 Locking the Markers 1008.5.5 Fixed Markers 1008.5.5.1 Adding and Removing Fixed Markers 1018.5.5.2 Automatically Mark Peaks 1018.5.5.3 Value of Markers 1028.5.6 Working with Data in Traces 1028.5.6.1 Active Trace 1028.5.6.2 Trace Update Options 1048.5.6.3 User-Defined Update Options 1058.5.6.4 Viewing Properties of a Trace 1068.5.6.5 Reordering Traces 1068.5.6.6 Math Operations 1118.5.6.7 Functional Traces (Traces Defined by a Function) 1118.5.7 Saving and Loading Data 1128.5.7.1 Saving Spectral Data 1138.5.7.2 Loading Spectral Data 1138.5.8 Analyzing data 1148.5.8.1 Floating Windows 1148.5.8.2 Displayed Range and Threshold 1168.5.8.3 Peak Track 1188.5.8.4 Valley Track 1198.5.8.5 Statistics Analysis 1218.5.8.6 Curve Fit 1248.5.8.7 Baseline Correction 1258.5.9 Keyboard Shortcuts 1268.5.10 General OSA Raman Settings 1268.5.10.1 Basic Settings 1288.5.10.2 Saving and Loading Settings 1318.6 Safety 

1328.7 Return of Devices 1328.8 Manufacturer Address 1328.9 Warranty 1328.10Copyright and Exclusion of Liability 1338.11Thorlabs Worldwide Contacts - WEEE Policy

We aim to develop and produce the best solutions for your

applications in the field of optical measurement techniques. To help

us to live up to your expectations and constantly improve our

products, we need your ideas and suggestions. We and our

international partners are looking forward to hearing from you.

Thorlabs GmbH

Warning

Sections marked by this symbol explain dangers that might result in

personal injury or death. Always read the associated information

carefully before performing the indicated procedure.

Please read this advice carefully!

This manual also contains "NOTES" and "HINTS" written in this form.

Attention

Paragraphs preceded by this symbol explain hazards that could

damage the instrument and the connected equipment or may cause

loss of data.

Note

Raman Spectroscopy Bundle Chapter 1: General Information

MTN022733-D02

Page 1

1 General Information

This manual describes the use of the Thorlabs components for Raman Spectroscopy, a spec-

trograph and different front ends, suitable for varying applications.

Raman spectroscopy is a technique that detects and analyzes inelastic photon scattering,

known as Raman scattering. Because Raman scattering is material specific and provides a mo-

lecular fingerprint, Raman spectroscopy can be used to identify specific chemicals or mo-

lecules.

One challenge in Raman spectroscopy has long been the low signal-to-noise ratio (SNR) due to

the very weak Raman scattering signal. The low SNR can be overcome with the use of Coded-

Aperture (CODA) technology: The CODA technology uses a two dimensional matrix with a

defined pattern of multiple slits instead of a single slit input aperture. This coded input aperture

with multiple slits allows data acquisition from a larger sampling volume than a single slit input

aperture. This in turn, enables higher sampling rates and an increased SNR. Please find more

general information on Raman spectroscopy and background on Coded-Aperture systems in

the chapter Operating Principle .

RSB1(/M) Raman Spectrograph

The Thorlabs RSB1(/M) Raman Spectroscopy Base Unit features such a highly optimized spec-

trograph based on the CODA technology with a pseudo Hadamard mask of order 64 and an at-

tached CMOS camera. The geometric size of the coded-aperture's active area is 3.159 mm x

2.304 mm. Due to the coded-aperture technology and the spatial averaging in the analysis, the

Thorlabs Raman spectrograph RSB1(/M) is superior in the analysis of complex mixtures.

Beyond the Coded-Aperture technology, the RSB1(/M) Raman Spectroscopy Base Unit is de-

signed for 785 nm excitation and a detection range of 500 cm-1 to 1800 cm-1. The RSB1(/M)

Base Unit can be purchased in an imperial (RSB1) or metric version (RSB1/M). For the purpose

of this manual, RSB1(/M) refers to both versions.

To assemble a full Raman spectroscopy system, the RSB1(/M) needs to be equipped with a

front end which collects the scattered Raman signal for detection and analysis, an excitation

light source at 785 nm, and a PC.

Front Ends RSBR1(/M) and RSBC1(/M)

Thorlabs offers different front ends for variable applications:

The RSBR1(/M) reflective front end is designed to examine powder and solid samples. It

carries the optical modules which guide the laser light to the sample and collect back-scattered

Raman signal from the sample and focus it onto the RSB1(/M) input aperture. For laser light in-

put, the RSBR1(/M) features an FC/PC connector. A sample table is provided to place a speci-

men at the optimal focal plane. The RSBR1(/M) even allows to analyze samples that are trans-

parently wrapped. The wrapping component can be eliminated from the output spectrum

provided that the focal volume is located within the sample.

The RSBC1(/M) cuvette front end for 10 mm cuvettes is designed to examine transparent,

translucent, or semi-transparent materials, since Raman scattered light is collected in a 90°

geometry with respect to excitation. Compared to the reflective front end, due to the 90° geo-

metry, this reduces the amount of scattered laser light or Rayleigh scattering that enters the

spectrograph. As for the RSBR1(/M), the laser is connected via a FC/PC connector.

Raman Spectroscopy Bundle Chapter 1: General Information

Both front ends can be purchased as an imperial version (RSBR1, RSBC1) or a metric version

(RSBR1/M, RSBC1/M). For the purpose of this manual, RSBR1(/M) or RSBC1(/M) refers to

both versions, respectively.

For custom-made solutions, Thorlabs offers the RSBA1 adapter to connect the RSB1(/M) to

Thorlabs 60 mm cage system components. Other custom-made front ends can be connected

to the RSB1(/M) using the SM1.5 thread at the input aperture.

For data acquisition and analysis, Thorlabs provides the OSA Software, Raman Edition

(Thorlabs OSA Raman Software). The software is shipped with the RSB1(/M) on an USB flash

drive which also contains all required drivers. For software updates, please see the chapter

Software and Installation .

As an excitation light source, Thorlabs recommends the FPV785M laser with the CLD1015

laser driver. The modular design of the Thorlabs Raman bundle also allows use of alternative

lasers, fulfilling the laser requirements stated in this manual.

Attention

Please find all safety information and warnings concerning this product in the chapter Safety

in the Appendix.

Please note that safety of the assembled system is the responsibility of the assembler. This

refers in particular to laser safety for high-power lasers as used for Raman spectroscopy.

1.1 Ordering Codes and Accessories

RSB1

Base Unit for Raman Spectroscopy (Imperial Threads) with Coded Aperture

(CODA Technology) and CMOS-Camera Sensor. Included are a MB1824

Breadboard with BBH1 Lifting Handles, RS2.5P Mounting Posts, CF125C

Clamping Forks, and a USB Stick Carrying the OSA Raman Software and

the Camera Calibration Data for Spectra Reconstruction.

RSB1/M

Base Unit for Raman Spectroscopy (Metric Threads) with Coded Aperture

(CODA Technology) and CMOS-Camera Sensor. Included are a

MB4560/M Breadboard with BBH1 Lifting Handles, RS2.5P/M Mounting

Posts, CF125C/M Clamping Forks, and a USB Stick Carrying the OSA Ra-

man Software and the Camera Calibration Data for Spectra Reconstruction.

RSBR1

Front End for Reflective Measurements of Solid Samples with the RSB1

Base Unit (Imperial Type). Included are a Sample Table for Imperial Sys-

tems with a Stray Light Shield and a RPC Polystyrene Calibration Chip.

RSBR1/M

Front End for Reflective Measurements of Solid Samples with the RSB1/M

Base Unit (Metric Type). Included are a Sample Table for Metric Systems

with a Stray Light Shield and a RPC Polystyrene Calibration Chip.

RSBC1

Front End for Measurements of Liquid Samples in a Cuvette with the RSB1

Base Unit (Imperial Type). Included are a TR1.5 Mounting Post and PH2E

131

Raman Spectroscopy Bundle Chapter 1: General Information

MTN022733-D02

Page 3

Post Holder, a CF125C Clamping Fork, 3500 ml Quartz Cuvette, and a RPB

Polystyrene Calibration Block.

RSBC1/M

Front End for Measurements of Liquid Samples in a Cuvette with the

RSB1/M Base Unit (Metric Type). Included are TR40/M Mounting Post and

PH50E/M Post Holder, a CF125C/M Clamping Fork, 3500 ml Quartz

Cuvette, and a RPB Polystyrene Calibration Block.

RSBA1

Adapter for Mounting of a 60 mm Cage System to the Input Aperture of

either RSB1 or RSB1/M.

Required Accessories:

Laser Driver CLD1015 is the Recommended Laser Driver

785 nm Laser We recommend the Thorlabs FPV785M laser. If using a different laser, please

see the recommended laser specifications Technical Data .

Replacement parts:

RPB Raman Spectroscopy Polystyrene Standard Block for Instrument Calibration

with the RSBC1(/M) Cuvette Measurement Front End.

RPC Raman Spectroscopy Polystyrene Standard Chip (Æ25 mm) for Instrument

Calibration with the RSBR1(/M) Reflective Measurement Front End.

CV10Q35F 3500 ml Macro Fluorescence Cuvette with Cap

To purchase the above replacement parts, please contact Thorlabs.

Recommended Accessories:

Safety Glasses

ADAFC1 Mating Sleeve: To connect the laser pigtail to the armored fiber patch

cable.

MR16L01 Armored Fiber Patch Cable: To connect the laser to the FC/PC port of the front

end.

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