Turner Designs Trilogy Bedienungsanleitung

Trilogy Laboratory Fluorometer

User’s Manual

Version 1.2

September 15, 2010

P/N 998-7210

Turner Designs

845 W. Maude Ave.

Sunnyvale, CA 94085

Phone: (408) 749-0994

FAX: (408) 749-0998

www.turnerdesigns.com

This Page Intentionally Left Blank

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 2 of 38

Highlights of the Trilogy Fluorometer

Multifunctional,

Compact and Modern

Design provides

flexibility and precision

Color Touch-Panel

Graphics User Interface

provides intuitive ease

of use

Sample Chamber accepts

snap-in Application Modules

for Fluorescence,

Absorbance and Turbidity

measurements

Trilogy saves time by storing

up to 18 named calibrations

Automatically calculates and

displays Extracted Chl a

concentrations using filtered

and solvent volumes

Spreadsheet Interface

Software provides real

time display and

logging of data to Excel

Spreadsheet

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 3 of 38

Table of Contents

CHAPTER DESCRIPTION PAGE

Highlights of the Trilogy Fluorometer 3

I. Getting Started 5

Description 5

Unpacking and Inspection 5

Setup 6

Getting to the Home Screen 6

Precautions 7

II. Fluorometer Operation 8

Touch screen Basics (Fluorescence) 8

Measuring Samples 9

III. Absorbance Operation 11

Touch Screen Basics (Absorbance) 11

Measuring Samples 12

IV. Turbidity Operation 14

Touch Screen Basics (Turbidity ) 15

Measuring Samples 15

V. Calibration Overview 16

V. Calibration Overview 17

A. Why Calibrate 17

B. When to Calibrate 17

C. Trilogy Calibration Options, (Fluorescence and Turbidity) 17

D. Trilogy Calibration Procedures 17

E. “Direct Calibration” Procedure – a) Fluorescence and Turbidity Modules, Single Point and Multi Point Cal 18

F. Extracted Chlorophyll Measurements with the Chl a– Acidification Fluorescent Module 20

G. Calibrating and Displaying Corrected Readings for the Chl aAcidification and Non-Acidification Modules 20

H. “Direct Calibration” Procedure – b) Extraction, Acidification, Single Point and Multi Point 20

J. “Direct Calibration” Procedure – c) Extraction, Non-Acidification, Single Point and Multi Point 23

K. Using the Secondary Standard 23

L. Using the Solid Secondary Standard for in vivo Chlorophyll Applications 23

M. Use of the Solid Secondary Standard for Dye Applications: 24

N. Trilogy Calibration Procedure, (Absorbance Module) 24

VI. Touch Screen Basics 26

Touch Screen 26

VII. Systems Connections 27

Serial Output Functional Check 27

VIII. Spreadsheet Interface Software Installation 28

Excel Spreadsheet Example 28

Appendix A - Troubleshooting 29

Fluorescence Troubleshooting 29

Absorbance Troubleshooting 29

Turbidity Troubleshooting 30

Spreadsheet Interface Software Troubleshooting 30

Appendix B - Warranty and Obtaining Service 32

Warranty 32

Out-of-Warranty Service 32

Appendix C – Specifications 33

Appendix D – Principles of Fluorescence 34

Principles of Fluorescence 34

Appendix E – Recommended Measurement Practices 35

Linear Range and Quenching 35

Temperature Considerations 35

Appendix F - Chlorophyll a Acidification and Non-Acidification calculations 36

Appendix G - In vivo fluorescence measurements using Trilogy 37

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 4 of 38

I. Getting Started

Description

The Trilogy Laboratory Fluorometer is a compact, multifunctional laboratory instrument that can

be used for making fluorescence, absorbance, and turbidity measurements using the appropriate

snap-in Optical Module. A color touch screen with simple menus makes for an intuitive user

interface.

Fluorescence Modules are available for discrete sample measurement of various fluorescent

materials including chlorophyll (in vivo and extracted), rhodamine, fluorescein, cyanobacteria

pigments, ammonium, CDOM, optical brighteners and other fluorescent compounds.

The Absorbance Module accepts interchangeable filter paddles so measurements can be made

at different wavelengths in order to identify or place a sample in a particular class of compounds.

The standard filter paddle wavelengths/bandwidths are: 560/10; 600/10 and 750/10 nm.

The Turbidity Module uses an Infrared (IR) LED with a wavelength of 850 nm as required for

reference method: ISO 7027/DIN EN 27027, “Water Quality – Determination of Turbidity”. Using

Infrared allows Turbidity to be measured at wavelengths that are not normally absorbed by

organic matter thereby reducing susceptibility to interference by sample color.

When properly calibrated, the Trilogy Fluorometer will read out the actual concentration of the

solution. Optical Modules contain the necessary light source and filters for the relevant

application

Unpacking and Inspection

Upon receiving the Trilogy, please inspect it carefully and make certain all accessories are

present. Refer to the checklist shipped with the instrument for order-specific items.

A typical Trilogy shipment includes:

- Checklist

- Trilogy Laboratory Fluorometer

- CD – Trilogy, includes files for: User’s Manual (pdf); Quick Start Guide (pdf);

Spreadsheet Interface Software (pdf)

- Solid Secondary Standard, (not intended for use with UV or Turbidity optical modules)

- Power Supply Kit

- RS-232 Cable

- Quick Start Guide, (hardcopy)

- 10 x 10 mm Methacrylate Cuvettes, (quantity of 10)

- 12 x 35 mm Glass Round Vial, (quantity of 5)

- 12 mm Round Adaptor

- 50-Well Microtube Storage Box

- Optical Module(s) as ordered

Fluorescence Module

Absorbance Module

Turbidity Module

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 5 of 38

Setup

Place the Trilogy Fluorometer on a flat, level surface. Allow at least 6 inches (16 cm) of clearance

above the instrument to open and close the lid. Position the instrument so that the touch screen

faces you .

Power Supply

Connect the power supply into the power connection of the instrument, see Figure 1, and plug it

into a wall outlet. See Specifications for power requirements.

Figure 1.

Getting to the Home Screen

1. With the unit turned off, lift the lid and insert an optical module into position, (see Fig 2).

Then press down on the module until you hear it snap into place. Be sure to close the

fluorometer lid so that the Trilogy power on step will complete successfully.

2. Turn on the On/Off switch located on the back of the Trilogy, see Figure 1. Verify the display

becomes active, and shows the module selection screen, see Figure 3 – Module Selection.

Figure 2 showing Optical Kit

installed in preparation for getting to

the Home Screen.

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 6 of 38

Figure 3 Module Selection Screen

3. Select the module inserted, and touch “OK” on the confirming screen.

4. When the Home Screen is displayed (Fig. 4), you are now ready to use Trilogy in its Raw

Mode (measurements are relative) or calibrate the Trilogy and the snapped-in Optical

Application Module to make quantitative measurements.

Figure 4: Trilogy Home Screen Display for Fluorescence Module Configuration.

Precautions

The Trilogy is intended for indoor use only. Wipe up spills immediately. The Trilogy contains

sensitive optical components and precision-aligned mechanical assemblies. Avoid rough

handling. Do not leave the lid open for extended periods of time. Turn OFF the Trilogy to change

or install Optical Application Modules.

Note: After 20 minutes without activity or user stimulation, the Touch Screen hibernates. Lightly

touch the screen to activate.

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 7 of 38

II. Fluorometer Operation

Fluorescence Optical Module

Installation

1. Power the Trilogy OFF

2. Grasp the handle of the Optical Application Module and align the kit with the sample

compartment.

3. Press down firmly to lock the Optical Application Module in place. You should hear or

feel a click indicating the module has snapped into place. (See Figure 5.)

4. Close the lid and power ON the Trilogy. Use the touch screen to identify the type of

Optical Application Module installed.

Figure 5.

Removal

1. Power OFF the Trilogy before removing the Optical Application Module

2. Grasp the handle and gently pull up to release the kit from the sample compartment.

Touch screen Basics (Fluorescence)

Home Screen

The "Home" screen appears after confirmation of the Optical Application Module. The "Home"

screen provides orientation for the multiple functions of the Trilogy. From the "Home" screen,

select "Calibrate," "Tools," "Mode," or "Help." The "Home" screen is also the measurement

screen. (See over for Figure 6 showing Home Screen with callouts).

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 8 of 38

Figure 6 – Trilogy Home Screen for Fluorescence Module Configuration.

Calibration

Refer to Section V.

Measuring Samples

There are two measurement modes available on the Trilogy when using the Fluorescence

Module:

Raw Fluorescence Mode – No calibration required

Direct Concentration Mode – Calibration required (see Section V)

Touch “Mode” on the Home Screen to select the measurement mode.

1. Raw Fluorescence Mode: The Raw Fluorescence Mode should be used for qualitative

measurement, relative changes in fluorescence rather than absolute concentration values.

Readings are displayed in Relative Fluorescence Units (RFU).

2. Direct Concentration Mode: The Direct Concentration mode makes absolute measurements

based on a calibration; see Section V for the Calibration Procedure.

The Trilogy accommodates 10 x 10 mm methacrylate and polystyrene cuvettes (minimum 2 mL

volume). Use 12 x 35 mm or 12 x 75 mm glass test tubes for measuring solvent applications,

such as extracted chlorophyll, and use methacrylate cuvettes for UV water applications.

1. Open the lid of the Trilogy and insert the cuvette. Close the lid.

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 9 of 38

2. Touch "Sample ID" to name your sample (optional).

3. Using the keypad, enter the sample name into the name field and touch "Save" to

save the sample ID.

4. Touch "Measure Fluorescence" to make a measurement. The Trilogy will measure

the sample for 6 seconds and report the average reading for the sample.

The Trilogy reports data on the "Home" screen and displays the results for the most recent 20

measurements. Use the arrow keys to scroll through the most recent measurements. The data

automatically exports to a printer or PC when properly connected, (see Section VII). Please note

the Trilogy does not store more than 20 measurements at one time. If more than 20 readings are

taken, the oldest reading will be overwritten. Measurements are not stored between power

cycles.

Tools (Fluorescence)

Touch the “Tools” key to access "Settings."

Tools - Settings

View Cal Details

Touch "View Cal Details" to see information on the current calibration for Direct Concentration

Mode. "View Cal Details" specifically provides information on the raw fluorescence for each

standard and the blank as well as the unit of measure and the Optical Application Module.

Continuous Sampling

The Continuous Sampling feature enables repeat measurements at user-defined intervals.

1. Touch "Continuous Sampling" and turn the feature ON. Highlight the frequency of

measurement and the number of total measurements. The maximum number of total

measurements is 9999.

2. Touch "OK" to return to the "Home" screen.

3. Connect the Trilogy to a printer or a PC to collect the data. Touching the screen

repeatedly causes an early-abort of Continuous Sampling measurements.

Measurement Tip:

On the Home Screen, touch Settings then touch the

Lid Start key to turn the feature ON. When the Lid

Start feature is ON, measurement begins as soon as

the lid closes. The lid start feature allows for immediate

measurement and eliminates the need to touch the

"Measure" key. Also, the touch screen does not

hibernate when Lid Start is ON.

Return to the Lid Start key under the Settings menu

to turn the feature OFF.

Trilogy Fluorometer User’s Manual P/N 998-7210 Page 10 of 38

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