VistaLab Ovation QS Handbuch

Operator’s Guide for

Adjustable Volume-Set Pipettes

(Ovation QS)

L057-0144-001 Rev. H

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide

Product Description & Specifications 1-1

Pipette Components 1-2

Introduction & Ergonomics 2-1

Ovation pipetting 2-3

Picking Up & Holding 2-3

Changing Volume Setting 2-3

Acquiring Tips 2-4

Aspirating and Dispensing 2-4

Ejecting Tips 2-5

Pipetting Hints for Optimal Performance 2-5

Introduction 3-1

Determining a New Calibration Factor 3-2

Entering a Calibration Factor 3-2

Cleaning Exterior 4-1

Disinfecting Nozzles 4-1

Replacing a Nozzle 4-1

Replacing a Nozzle Filter 4-2

Plunger Assembly Seals 4-3

Replacing the Battery 4-3

Troubleshooting 4-4

Tips 5-1

Warranty, Safety Compliance & Contact Information A-1

eneral Product Information A-2

TABLE F C NTENTS

Introduction

BioNatural

Pipetting

Calibration

Maintenance &

Troubleshooting

Tips & Accessories

Troubleshooting

Appendix A

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 1 - 1

Ovation BioNatural™pipettes are the ideal ergonomic solution for liquid handling

tasks that contribute to repetitive stress injuries in

today's laboratory. Ovation

pipettes increase laboratory efficiency through improved

liquid handling routines,

while reducing fatigue and the effect of poor postures.

The Ovation QS is intended for use as a liquid transfer device, capable of aspirating

and dispensing precise fluid volumes.

This manual describes how to use and care for your Ovation pipette. As you can see

from its appearance, it is different from every pipette that you have used before,

therefore please read the instructions carefully.

Six models of the Ovation pipette are available to cover liquid dispensing needs

ranging from 0.2µL to 1000µL.

Please activate the pipette’s warranty on the VistaLab Technologies web site –

www.vistalab.com. The required on-line form can be found in the “service” menu.

Retain all packing materials in the event that the pipette is to be sent back to VistaLab

Technologies for calibration verification or service.

Product Description

Specifications

VATI N QS ADJUSTABLE V LUME-SET PIPETTES

Volume Dispensing

Range Increments

Model (µL) (µL) Accuracy Precision

vation QS 2µL 0.2 – 2 .002 ±1.5% at 2µL 0.9% at 2µL

(1057-0002) ±2.7% at 1µL 1.8% at 1µL

±9% at 0.2µL 7.6% at 0.2µL

vation QS 10µL 1 – 10 .01 ±1% at 10µL 0.4% at 10µL

(1057-0010) ±1.5% at 5µL 0.6% at 5µL

±2.5% at 1µL 1.2% at 1µL

vation QS 20µL 2 – 20 .02 ±1% at 20µL 0.3% at 20µL

(1057-0020) ±1.5% at 10µL 0.5% at 10µL

±7% at 2µL 2% at 2µL

vation QS 100µL 10 – 100 .1 ±0.8% at 100µL 0.15% at 100µL

(1057-0100) ±0.8% at 50µL 0.24% at 50µL

±3.5% at 10µL 1% at 10µL

vation QS 200µL 20 – 200 .2 ±0.8% at 200µL 0.15% at 200µL

(1057-0200) ±0.8% at 100µL 0.25% at 100µL

±2.5% at 20µL 1% at 20µL

vation QS 1000µL 100 – 1000 1.0 ±0.8% at 1000µL 0.15% at 1000µL

(1057-1000) ±0.8% at 500µL 0.2% at 500µL

±3% at 100µL 0.6% at 100µL

Note: Performance data is based on the use of Ovation tips from VistaLab Technologies. If using

tips from another manufacturer, the above performance claims may not be achieved.

Specifications subject to change. See www.vistalab.com for current information.

Ejec or Sleeve

Nozzle

Volume Adjus men UP Body

Tip Ejec Bu on

Adjus able

Hook

Plunger

Selec Bu on

Volume Adjus men DOWN

LCD Screen Display

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 1 - 2

Description Function

Plunger Initiates pipetting action and changes volume setting

Tip Eject Button Ejects disposable pipette tips with minimal thumb force

Adjustable Hoo Accommodates left and right handed users

Ejector Sleeve Stored energy from tip installation releases tips

Nozzle Tapered, chemically resistant coupling that secure pipette tips

Body Contoured grip surface for comfort, eliminating tight hand grip

Volume Adjustment UP Adjusts volume setting up

Select Button Allows user to step through pipette’s menu

Volume Adjustment DOWN Used to easily open PCR and microtubes

LCD Screen Display Displays curent setttings

Pipette Components

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 2 - 1

BI NATURAL PIPETTING

Studies have shown that pipetting is the #1 cause of musculoskeletal disorders in the

laboratory – it is, by nature, a repetitive process that puts strain on the body. While

we can’t change how repetitive pipetting is, we can and did change how comfortable

you are while doing it.

The Ovation BioNatural Pipette is the only pipette designed to keep your hand in the

neutral position recommended by ergonomics experts. We call this BioNatural™

pipetting – it allows a lower hand location to ease stress in the shoulder, and a

relaxed wrist angle eliminates uncomfortable extension and radial deviation

movements in the arm. Force, velocity and exertion from repetition or duration have

also been neutralized because of the Ovation pipette’s unique working position and

reduced forces required during operation.

Some practice may be required to change years of posture and habits developed

using standard axial pipettes; the physical benefits of BioNatural pipetting are worth

the practice! When using the Ovation pipette, arm/hand elevation should remain low

to the work surface, wrist rotation should not exceed 90º, and hand posture should

remain relaxed with the wrist and back of hand slightly flexed.

Introduction

Posture

Arm elevation remains low,

minimizing stress to elbow,

shoulder and neck

A loose, relaxed grip increases

available strength in the hand,

improving endurance and

productivity during pipetting

Wrist remains in a neutral

range of motion throughout

all pipetting operations

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 2 - 2

Ergonomics experts recommend establishing a “comfort zone” of

movement for the task of pipetting. Position your body within 9” of the

counter surface, and keep the items needed within easy reach. Ideally this

should be 14” or less, and should not exceed 24” of occasional reaching.

Wrist rotation should not exceed 90˚ from the work surface, and arm/hand

elevations should remain low to the counter surface.

Keep a “comfort zone”

for pipetting

14”

Maximum

Reach

“Comfort Zone”

14”

24”

5. Low arm and

elbow height

4. Neutral forearm

and wrist rotation

3. Relaxed hand grip

2. Low thumb forces

1. Relaxed shoulders

and upper arms

vation comfort-zone benefits:

1. Minimized tension and fatigue

2. Minimized exertion and contact stress

3. Low contact pressure

4. Lowest pressure in carpal tunnel and to median nerve

5. Minimized tension to shoulder and elbow

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 2 - 3

The Ovation QS has been designed to

allow the body of the pipette

to fill your

palm. Rotate the adjustable

hook to rest

comfortably on your

forefinger. The

texture of the unit’s back

helps reinforce

correct hand positioning.

The unique ergonomic design and

adjustable hook is compatible for both

right and left-handed use.

Keep hand posture relaxed – there’s no

need for a “firm grip” when using an

Ovation pipette.

One Ovation pipette takes the place of

many regular pipettes. That’s because

your laboratory’s most frequently-used

volumes can be entered and stored for

easy recall and use at any time. Any

precise volume throughout the pipette’s

volume range can also be selected.

To change a volume setting, press the

or buttons until the desired volume

appears on the LCD display (volume

display will flash until it is “Locked In”).

To “Lock In” a selected volume setting

depress the plunger to the first stop and

hold until three “beeps” are heard. The

volume display stops flashing and Ovation

is ready for use at the new setting.

The Ovation pipette comes with five (5)

pre-programmed volumes. These are

identified on the LCD as 1 through 5.

You can change any or all of these pre-

programmed volumes to those

frequently used in you laboratory.

To select a pre-programmed volume

setting, press the button until the

desired volume appears on the LCD.

“Lock In” this setting by holding the

plunger down at the first stop until three

“beeps” are heard.

To modify a pre-programmed volume

setting, press and release the button

until the volume setting to be changed

appears on the LCD. Press the or

buttons to scroll the display to the new

volume setting (volume display will be

flashing). Press and hold the button

(approximately 3 seconds) until a “chirp”

is heard. This “locks in” the new setting.

Picking Up the

Pipette

Changing

Volume Setting

Select Button

Volume DOWN

Volume UP

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 2 - 4

While maintaining a flat wrist-hand

posture, insert the Ovation pipette

nozzle into a tip. Press down until you

hear or feel a “click”. This “click”

indicates that the tip is properly seated

and ready for use.

Disposable pipette tips are to be used

once, then discarded. For proper fit and

to achieve stated precision and accuracy

specifications, use the VistaLab pipette

tips recommended in this manual. Use of

other manufacturer’s tips can cause the

pipette performance to differ from stated

specifications, can damage the nozzle or

ejector sleeve, or impede the performance

of the tip ejection system.

1. Press the plunger down to the first

stop, and immerse pipette tip in the

sample.

2. Smoothly and slowly, release the

plunger allowing sample to enter the

pipette tip. Wait one second before

withdrawing the tip from the sample.

3. Place the pipette tip against the side

of the receiving vessel close to the

bottom of the vessel, or if it contains

liquid, just above the surface of the

liquid.

4. Smoothly depress the plunger to the

first stop. Wait one second, then fully

depress the plunger to the second

stop to dispense all liquid from the tip.

5. With the plunger depressed, slowly

withdraw the tip.

6. Release the plunger when the tip is

away from the receiving vessel.

See “Pipetting Hints for Optimal

Performance” for additional information.

Acquiring Tips

Aspirating and

Dispensing

Tip Immersion Depth

0.2µL to 10µL 1mm

11µL to 100µL 2-3mm

101µL to 1000µL 2-4mm

2 -5

Ovation pipettes store energy captured

during the acquisition of a tip, and use

it to discard tip when the tip eject button

is pressed. Point pipette tip into a

suitable

waste container and press the

tip eject button to discard the tip.

To minimize forearm elevation and

“reaching”, position the waste container

below worksurface level or use shallow

containers. Raising the arm to discard

tips into a tall receptacle on the

counter may negate some of the

benefits of Ovation’s low-profile design.

Ejecting Tips

• When using viscous or volatile reagents, pre-wetting the pipette tip may be

appropriate. To pre-wet, aspirate and dispense the liquid back into the original

vessel. Then fill the tip and dispense contents into receiving vessel.

• If an air bubble forms in the tip during aspiration, return the sample, discard the

tip, and use a fresh tip.

• Smoothly depress and release the plunger when pipetting, maintaining the same

speed of motion for all samples. Do not let the plunger “snap back” to the

uppermost position.

• When finished aspirating, touch the pipette tip against the side of the vessel as it

is being withdrawn to remove any liquid that may have adhered to the outside of

the tip. Wipe the pipette tip ONLY if there is liquid adhering to the outside. Be

careful not to “wick out” any of the contents from the pipette tip.

• Dispense against the side of the receiving vessel or above the liquid surface. To

remove any of the measured liquid that may have adhered to the pipette tip,

touch off the tip against the side of the receiving vessel.

•

Optional Type A nozzles for 100–1000µL models are designed to accommodate

some non-VistaLab tips.

•

If aspirating from 15mL or 50mL centrifuge or other tall tubes, an optional Nozzle

Extender accessory is available which lengthens the pipette’s overall reach.

If the

optional nozzle extender is being used, calibration verification should be checked.

Pipetting Hints for

ptimal Performance

Ovation pipette with Nozzle Extender

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide

Ovation QS BioNatural Pipette – Adjustable Volume-Set Operator’s uide 3 - 1

CALIBRATI N

Each Ovation pipette is factory calibrated to manufacturing specifications at 21.5˚C

(±2˚) using distilled water. It is recommended that performance be verified at least

every six (6) months, when internal maintenance has been performed, or on an as-

needed basis.

The Ovation pipette can be easily in-lab calibrated ±10% for optimum performance

for your operating conditions. In addition, the calibration factor can be pre-determined

and set at the appropriate value for a liquid to be dispensed. Changing the calibration

factor is quick and easy. Returning the calibration factor to 1.000 will re-set the

Ovation pipette to its original factory calibration.

Prior to performing calibration procedures, “home” the plunger by simultaneously

pressing and holding the and buttons until appears on the LCD.

Depress the plunger to the first stop, and release it. Then depress the plunger to

the first stop again and hold until three “beeps” are heard.

For optimum performance over the entire pipetting range, verify and calibrate the

pipette using the maximum volume setting. Note: Unless using a specific liquid at

one volume, always verify and calibrate at the maximum volume.

With the pipette set to the desired volume, determine the actual volume dispensed*.

Then use the Ovation’s calibration function to determine the appropriate calibration

factor.

It is recommended that whenever the calibration factor is changed from the factory

setting, that the current factor be recorded on a calibration label on the base of the

pipette. The user may also wish to record the new factor in the laboratory’s quality

control log.

* For additional information on calibration verification, call VistaLab Technical

Services or go to www.vistalab.com

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