Wealtec CB-1 Bedienungsanleitung

Block Cooler System

(CB-1)

Installation and Operation Manual

Version 1.0

Item# 03060

*This instrument is intended for laboratory use only

Block Cooler V1.0

Index

A. Important Notice ---------------------------------------------------------------- 1

A-1. Warranty

A-2. Technical and Service Contact

A-3. Safety Notice

A-3-1. Certification

A-3-2. Safety Information

B. Introduction ---------------------------------------------------------------------- 3

B-1. Specifications

B-2. Product Description

B-2-1. Block Cooler System Hardware Overview

C. Installation of Block Cooler System (CB-1) ----------------------------- 9

C-1. Package List

C-2. Installation of Block Cooler System

C-2-1. Installation Procedure

D. Operation ------------------------------------------------------------------------ 11

E. OFFSET Function -------------------------------------------------------------- 12

F. Troubleshooting ---------------------------------------------------------------- 14

F-1. General symptom

F-2. Installation of Block Cooler System

G. Care and Maintenance -------------------------------------------------------- 16

H. Order Information -------------------------------------------------------------- 17

Block Cooler V1.0

A. Important Notice

Before setting up and operating the instruments of Block Cooler (CB-1) system, please

carefully read these instructions to get familiarized with the installation and operation process.

Instructions should be read by experienced individuals before operating the instruments.

Any improper usage of the instruments may cause damage. Please refer to the safety

notice included with this equipment.

The instruments shall not be modified or altered in any way. Any modification or alteration

will void the warranty, void the regulatory certifications and create potential safety hazard.

Wealtec is not responsible for any injury or damage caused by using the instruments for any

non-intended purpose or injury as a result of modification of the instruments by any person who

is not authorized by Wealtec Corp.

A-1. Warranty

Block Cooler system is warranted to be free from defects in materials or workmanship for a

period of one year from the original invoice date, under normal usage. Any defects occurring

during warranty period, Wealtec Corp. will repair or replace defective products or parts without

charge unless the defects arise from conditions outlined below. The defects described below are

specially excluded from Wealtec warranty policy.

1. Improper operation of the instrument.

2. Repair or modification by any person who is not authorized by Wealtec Corp.

3. Damage caused by any (in)-direct accident, neglect or misuse.

4. Damage caused by disaster.

5. Damage caused by any improper solvents or samples

A-2. Technical and Service Contact

Most of the operation details are described in this instruction manual to assist and guide

operator for an appropriate solution. For any other technical/ service questions, please contact

your local representative or contact Wealtec international technical/ service specialist by E-mail:

[email protected].

Block Cooler V1.0

A-3. Safety Notice

A-3-1. Certification

The block cooler system is designed to meet the international electrical safety standards

EN61010-1 and EMC regulations. This product designed to meet CE requirements and if

operated according to the guidance of the instruction manual, is certified safe. Any modification

or alteration will void the warranty, void the regulatory certifications and create potential safety

hazards.

A-3-2. Safety Information

¾Do not apply more or lower than the rated voltage specified on the instrument or it may

cause damages to the instrument.

¾Do not splash water or liquid onto the block cooler system, the spilled water or liquid

may cause malfunction to the block cooler system.

¾Do not touch or remove the 48-Well aluminum block directly with hand while heating,

cooling or when the block is still hot or cold.

¾Do not directly pour water/liquid/ice on the high or low temperature heating block to

cool down or stabilize the temperature of the block to the room temperature. It may

cause damage to the instrument and fast-evaporated water/liquid may be harmful to

the operator.

Block Cooler V1.0

B. Introduction

Block Cooler System (CB-1) is a standard cooling unit for laboratory use. The compact

cooling unit temperature ranging 0 – 75℃allows customizing settings for specific temperatures

or utilizing the nine frequently used pre-set temperatures for various molecular biology or

chemistry applications. The universal 48-well aluminum block is a single block designed to suit

various types of microtubes without the need of the multiple blocks. An applicable samples for

block cooler accordingly for various temperatures is given in the table below:

Selectable Temperature

Range Recommended Applications

0 °C Replace the ice buckets for samples & enzymes storage;

4 °C

Sample cooling & storage during operation –

i.e: Store nucleic acid at 4°C in long PCR at final stage, DNA

purification kit rinsing and sample with 70% ethanol at 4°C,

hybridization process when adding polyAto hold at 4°C in nucleic

acid.

cDNA synthesis experiment –

i.e: Placing oligonucletides or polymerase at 4°C.

Pre-thaw the primary antibody for continuous uses –

i.e: western blotting procedure.

Enzyme reaction –

i.e: ligation steps of gene construction.

Cell lysis experiment –

i.e: Resuspend the protein in lysis buffer at 4°C.

General immunoprecipitation procedure.

16 °C Enzyme reaction –

i.e: Restriction enzyme activity for DNAligation.

25 °C

Biological response assay.

Enzyme catalysis activity reaction.

In long PCR reaction at final stage “hold”.

Block Cooler V1.0

37 °C

In cell culture procedure to dissociate the cell with

Typsine/DNAase I solution in HBSS medium or resuspend with

DNase I solution only in DMEM medium.

The denature DNAsteps in DNA extraction kit.

DNA/RNA probe preparing.

The incubation steps in cDNA synthesis.

Bacteria culture incubation or purification.

Enzyme reaction –

i.e: Restriction enzyme reaction and RNAase reaction by DNA

extraction or Flow cytometry ofApoptosis.

Incubate the plug at 37°C in Pulsed Field Gel Electrophoresis.

For gel digestion pre-treatment before MALDI-TOF MS analysis.

42 °C

The reverse transcription temperature by RT-PCR.

Enzyme reaction –

i.e: DNA ligase gene reaction for thermosensitive mutant

application.

The incubation procedure of phage purification.

Gene transformation incubation –

i.e: Transfer gene to E. Coli strain for expression.

55 °C

The steps required on cDNA synthesis.

Chemical reaction –

i.e: The gentle heating steps by DNAextraction kit solution.

Enzyme reaction –

i.e: RNAase reaction.

Gel digestion –

i.e: The oligonucleotides purify from SDS-PAGE gel.

Block Cooler V1.0

65 °C

In RT-PCR experiment, continuous to hydrolysis steps.

Inactivate enzyme reaction –

i.e: Inactivate the proteinase K reaction in DNA/RNAprobe

preparing.

The incubation temperature in Bacteriophage DNApurification

procedure.

Keep the melted Agar / Agarose medium warm to extract nucleic

acid.

72 °C

Chemical / enzyme reactions –

i.e: Inactivate enzyme reaction.

Sample dissolving in solution –

i.e: Agarose dissolving.

Denatured sample –

i.e: Pre-heating protein molecules before SDS-PAGE running.

Customize Temperature

Between 0-75 °C

Specific incubation temperatures for chemistry / molecular biology

experiment requirements.

Sample & chemical dissolving.

B-1. Specifications

Temperature range 0 – 75℃

Number of pre-set

temperature 9 Points (0, 4, 16, 25, 37, 42, 55, 65 and 72℃)

Temperature accuracy +/- 0.1℃

(Full temperature range except at 25℃)

Cooling performance 35 minutes (From 25℃to 0℃)

Temperature controller Microprocessor with PID algorithm

Temperature

control mode Digital

Temperature calibration Built-in

Display type LCD display

Block Cooler V1.0

Heating /

Cooling element Peltier

Heating power (Watt) 75 max.

Over temperature cutoff 100℃

Power 90–264 VAC, 47–63 Hz

Dimension (L x W x H) 200x304x184 mm (7.87”x11.97”x7.24”)

Weight

(With block) Net~6.5kg (14.3 pounds)

Gross~7.5kg (16.5 pounds)

Block capacity 1

Operating conditions Temperature : 0 – 26 ℃

Humidity : 10% to 90% R.H. Non-condensing

Aluminum block

Block type 48-Well aluminum block

Well type 8 x 0.2 ml 21 x 0.5 ml 15 x 1.5 ml 4 x 2.0 ml

Number of holes 8 21 15 4

Tube type 0.2 ml

microtube 0.5 ml

microtube 1.5 ml

microtube 2.0 ml

microtube

Hole diameter (mm) 6.5 8 11 13

Hole depth (mm) 20 29 34.5 34.5

Block material Solid anodized aluminum

Dimension (L x W x H) 107x71x38 mm (4.22”x2.8”x1.5”)

Block Cooler V1.0

B-2. Product Description

B-2-1. Block Cooler System Hardware Overview

Control panel

¾“RUN/STOP” key – Press this button in order to run or stop the block cooler.

¾“OFFSET” key – Press this button and adjust the offset value in order to alter the

temperature of the block cooler system to the desired temperature as per to users

Process indicato

Power switch

Protection lid

Protection lid handle

48-Well aluminum block “Run/Stop” key

“Offset” key

Ready indicator

Heating indicator

Power indicato

Coolin

indicato

LCD display

Up or down key

for selected parameter

Block Cooler V1.0

calibrated thermometer.

¾Power indicator will turn on if the block cooler system is turned on.

¾“ ” – Cooling indicator will turn on if the system is desired to cool down.

¾“ ” – Heating indicator will turn on if the system is desired to heat up. In the other

word when the set temperature equals to the actual block temperature.

¾“ ” – Ready indicator will turn on if the system has reached the desired

temperature. In the other word when the set temperature equals to the actual block

temperature.

¾“ ” – Process indicator will turn on while the system is progressing to the desired

temperature.

¾“ ” & “ ” – Up and down key is used to adjust the desired temperature value

and the desired mode of the temperature.

¾LCD display – Displays the temperature value and the mode settings. LCD display

allows for easy system setting operation and monitoring.

Protection lid

Protection lid design avoids possible contaminations and environmental heat transfer. This

indirectly helps to keep and maintain the desired temperature of the block cooler system which

could be influenced from the environment. Protection lid handle provides easy handling of the

protection lid.

48-Well aluminum block

48-Well aluminum block is a single block suitable for various types of microtubes which

includes 4 x 0.2ml, 21 x 0.5ml, 15 x 1.5ml and 4 x 2.0ml.

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