Agilent Technologies InfinityLab 1290 Infinity II Bedienungsanleitung
Agilent InfinityLab
Evaporative Light Scattering Detectors
User Manual
Agilent InfinityLab ELSD User Manual
Agilent InfinityLab ELSD User Manual
Notices
Document Information
Document No: D0013647 Rev. A
Edition: 08/2022
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© Agilent Technologies, Inc. 2022
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Agilent InfinityLab ELSD User Manual 3
In This Book
This manual contains information on:
•Agilent 1260 Infinity II ELSD (G4260B)
•Agilent 1290 Infinity II ELSD (G7102A)
1 Introduction to the Detector
This chapter gives an introduction to the module, instrument overview, and internal
connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical, and
performance specifications.
3 LAN Configuration
This chapter provides information on connecting the module to the control
software.
4UsingtheModule
This chapter explains the operational parameters of the ELSD.
5 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
6Troubleshootingand Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions on how to recover from error
conditions.
Agilent InfinityLab ELSD User Manual 5
Contents
1 Introduction to the Detector 7
Introduction to the ELSD 8
System Overview 9
2 Site Requirements and Specifications 16
Pre-installation Requirements 17
Site Requirements 18
Physical Specifications 21
Performance Specifications 23
3 LAN Configuration 27
What You Have to Do First 28
TCP/IP Parameter Configuration 29
PC and OpenLab CDS, OpenLab ChemStation or OpenLab EZChrom
Software Setup 36
4UsingtheModule41
Before Using the Detector 42
Instrument Controls 43
Operational Parameters 48
Controlling the ELSD During an Injection 52
Controlling the ELSD within OpenLab CDS, OpenLab ChemStation or
OpenLab EZChrom 54
General Considerations 55
5 Optimizing Performance 59
Do’s and Don’ts of ELS Detection 60
Location of the Detector Module 61
Pumping Systems 62
Mobile Phase Priming 63
Agilent InfinityLab ELSD User Manual 6
6 Troubleshooting and Diagnostics 64
Troubleshooting 65
Troubleshooting an HPLC System 66
General Problems 67
7 Error Information 74
What Are Error Messages 75
Module Specific Error Messages 76
8 Maintenance 83
Introduction to Maintenance 84
Cautions and Warnings 85
Cleaning the Module 87
Inspection of Cables 88
Drying the Diffuser 89
Cleaning the Nebulizer 90
Cleaning Evaporator Tube 91
Putting the Instrument into Storage 92
Updating Detector Firmware 93
9 Parts and Materials for Maintenance 98
Identifying Parts and Materials 99
10 Appendix 100
General Safety Information 101
Waste Electrical and Electronic Equipment (WEEE) Directive 105
Radio Interference 106
Agilent Technologies on Internet 107
1 Introduction to the Detector
Introduction to the ELSD
Agilent InfinityLab ELSD User Manual 8
Introduction to the ELSD
The Evaporative Light Scattering Detector is a unique and highly sensitive
detector for semi-volatile and non-volatile solutes in a liquid stream. It is mainly
used as a concentration detector for High Performance Liquid Chromatography
(HPLC). The solvent stream containing the solute material is nebulized and
carried by a gas flow through an evaporation chamber. The solvent is volatilized,
leaving a mist of solute particles that scatter light to a photosensitive device. The
signal is amplified and a voltage output provides the concentration of the solute
particles passing through the light.
The Agilent 1260 Infinity II ELSD or Agilent 1290 Infinity II ELSD may be used
alone, or as one of several detectors in a GPC or HPLC system. As the solvent or
eluent is evaporated during the analysis, the Agilent 1260 Infinity II ELSD or
Agilent 1290 Infinity II ELSD must be the last in series if used with other
detectors. If the Agilent 1260 Infinity II ELSD or Agilent 1290 Infinity II ELSD is
being used as the last detector in a series, care must be taken not to exceed the
recommended backpressure in detector cells in other units.
This manual instructs the user in the installation and operation of the Agilent
1260 Infinity II ELSD or Agilent 1290 Infinity II ELSD for standalone use and
control using Agilent OpenLab CDS, OpenLab ChemStation, or OpenLab
EZChrom.
Agilent InfinityLab ELSD User Manual 9
1 Introduction to the Detector
System Overview
System Overview
Basic Principles of Operation
Nebulization
The eluent inlet is connected to the nebulizer via a short length of stainless steel
capillary tube. The incoming eluent stream passes through the heated nebulizer
and is mixed with the incoming nebulizer gas stream. The mixed gas and eluent
stream form an aerosol plume containing a uniform dispersion of droplets that
then passes as a continuous flow into the evaporator section. Any larger droplets
or the inefficiently nebulized droplets collect in the nebulizer chamber waste trap
and then drain off via the waste outlet into a collection bottle.
Evaporation
After nebulization, the atomized spray is propelled through the evaporation tube
assisted by the carrier gas. In the evaporator section, the solvent is removed
leaving a stream of dry particles of the analyte. A diffuser located in the
evaporator assists in the drying of the particles, acting as an efficient heat
exchanger, prevents ballistic particles reaching the scattering chamber and
randomizes the particle plume. The ELS Detector uses patented gas flow
technology in the evaporation zone to aid evaporation at low temperatures. By
adding a stream of dry nitrogen (evaporation gas) at the entrance of the
evaporator tube less volatile solvents (for example, water) are easily evaporated.
This evaporation gas is controlled by the user and facilitates subambient
operation.
1 Introduction to the Detector
System Overview
Agilent InfinityLab ELSD User Manual 10
Detection
Light in the optical chamber is passed through the instrument at right angles to
the direction of particle flow. A light trap is located opposite the source of light to
capture the transmitted incident beam eliminating internal reflections within the
instrument body. When pure solvent is being evaporated, only its vapor passes
through the light path and the amount of light scattered to the photomultiplier is
small and gives a constant baseline response. When a nonvolatile solute is
present a particle cloud passes through the light path, causing light to be
scattered. This scattered light enters the optical aperture of the detection system
and generates a signal response from the photodiode in real time. The quantity
of light detected depends on the solute concentration and solute particle size
distribution.
Figure 1 Principles of Operation
1. Nebulization 2. Evaporation 3. Detection
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