GE Monitor UV-900 Bedienungsanleitung

ÄKTA™

Monitor UV-900

User Manual

GE Healthcare

Important user information

All users must read this entire manual to fully under-

stand the safe use of Monitor UV-900.

WARNING!

The WARNING! sign highlights instructions

that must be followed to avoid personal

injury. It is important not to proceed until

all stated conditions are met and clearly

understood.

CAUTION!

The Caution! sign highlights instructions that must be

followed to avoid damage to the product or other equip-

ment. It is important not to proceed until all stated con-

ditions are met and clearly understood.

Note

The Note sign is used to indicate information important

for trouble-free and optimal use of the product.

CE Certifying

This product meets the requirements of applicable CE-

directives. A copy of the corresponding Declaration of

Conformity is available on request.

The

symbol and corresponding declaration of confor-

mity, is valid for the instrument when it is:

– used as a stand-alone unit, or

– connected to other CE-marked GE Healthcare

instruments, or

– connected to other products recommended or

described in this manual, and

– used in the same state as it was delivered from GE

Healthcare except for alterations described in this

manual.

Recycling

This symbol indicates that the waste of

electrical and electronic equipment must

not be disposed as unsorted municipal

waste and must be collected separately.

Please contact an authorized representa-

tive of the manufacturer for information

concerning the decommissioning of equip-

ment.

WARNING!

This is a Class A pruduct. In a domestic environment this

product may cause radio interference in which case the

user may be required to make adequate measures

Monitor UV-900 User Manual 18-1120-05 Edition AF

Contents

1 Introduction

1.1 General ................................................................................................7

1.2 Safety ...................................................................................................8

2 Installation

2.1 Unpacking ..........................................................................................9

2.2 General precautions .....................................................................9

2.3 Installing the flow cell ................................................................ 10

2.3.1 Fixing the flow cell .................................................................... 10

2.3.2 Connecting the optical fibers ............................................... 11

2.4 Connecting electrical signal cables .................................... 12

2.4.1 Connecting to recorder (if used).......................................... 12

2.5 Connecting to communication link ..................................... 12

2.6 Connecting to supply voltage ............................................... 13

2.7 Connecting the tubing .............................................................. 13

2.8 Mounting the cell holder cover ............................................. 14

3Operation

3.1 On/off ................................................................................................ 15

3.2 Menu selection and settings .................................................. 15

3.2.1 Menu selection ........................................................................... 15

3.2.2 Return to main menu .............................................................. 16

3.2.3 Select value.................................................................................. 16

3.3 Main menu overview ................................................................. 17

3.4 Setting lamp on/off ..................................................................... 17

3.5 Reading absorbance values ................................................... 18

3.6 Setting wavelength ..................................................................... 18

3.7 Autozero .......................................................................................... 19

3.8 Storage and shut-down ........................................................... 19

3.9 Using an external chart recorder ........................................ 20

3.9.1 Setting range and zero ........................................................... 20

3.9.2 Event mark ................................................................................... 20

3.10 Filtering noise ................................................................................ 21

3.11 UV cell calibration ....................................................................... 22

3.12 Changing flow cell ....................................................................... 24

3.13 Restart after power failure ...................................................... 24

4 Maintenance

4.1 Periodic maintenance ............................................................... 25

Contents

Monitor UV-900 User Manual 18-1120-05 Edition AF

4.2 Cleaning-in-place .........................................................................25

4.3 Checking the instrument ..........................................................26

4.3.1 Lamp intensity ............................................................................26

4.3.2 Lamp on–time .............................................................................26

4.3.3 Flip time..........................................................................................26

4.3.4 UV flow cell ...................................................................................26

4.4 Cleaning the flow cell and optical fiber connectors ....27

4.4.1 Cleaning the flow cell...............................................................27

4.4.2 Cleaning the optical fiber connectors...............................27

4.4.3 Instrument housing...................................................................27

4.5 ..................................................................Cleaning the system 27

4.6 Recycling ..........................................................................................28

5 Trouble-shooting

5.1 General .............................................................................................29

5.2 Faults and actions .......................................................................29

5.3 Error messages .............................................................................30

Reference information

A Description ........................................................................................................ 31

A.1

Instrument

............................................................................................................31

A.2

Flow cell

.................................................................................................................32

A.3

Monitor principle

................................................................................................32

B Menus .................................................................................................................. 34

B.1

Check menu

.........................................................................................................34

B.2

Setup menu

..........................................................................................................36

B.3

Setting and using the alarm timer

.............................................................37

B.4

Service displays

..................................................................................................37

B.5

Menu overview

...................................................................................................38

C Technical specifications ............................................................................. 39

D Accessories and spare parts ....................................................................42

Short instructions on side 39

About this manual

This manual comprises two parts; a practical part

(sections 1–5) and a reference part (sections A-D).

Sections 1–5 contain the necessary information for operating the

instrument.

Introduction 1

Monitor UV-900 User Manual 18-1120-05 Edition AF

1 Introduction

1.1 General

Monitor UV-900 is a UV-absorption monitor for use in liquid chromatography.

Monitor UV-900 features:

• Variable wavelength for detection in the range 190–700 nm.

• Up to 3 wavelengths can be monitored simultaneously.

• Two alternative flow cells with path length 2 mm and 10 mm.

• Accurate and reliable monitoring through self-test, self-calibration and

fiber optics.

Monitor UV-900

1Introduction

Monitor UV-900 User Manual 18-1120-05 Edition AF

1.2 Safety

• The instrument is designed for indoor use only.

• Do not use in a dusty atmosphere or close to spraying water.

IMPORTANT!

Monitor UV-900 is intended for laboratory use only, not for

clinical or in vitro use, or for diagnostic purposes.

WARNING!

When using hazardous chemicals, all suitable protective

measures, such as protective glasses, must be taken.

WARNING

! The instruments must not be opened by the user. They contain

high voltage circuits which can give a lethal electric shock.

WARNING

! The system must be connected to a grounded mains socket.

WARNING

! Always disconnect the power supply before attempting to

replace any item on the instrument.

WARNING!

The instrument uses high intensity ultra-violet light. Do not

disconnect the optical fibers while the monitor is running.

CAUTION!

The flow cell must not be used at pressures above 2 MPa (20 bar,

290 psi). At higher pressures the flow cell may break.

WARNING!

When using hazardous chemicals, take all suitable protective

measures, such as wearing protective glasses and gloves resistant to the

chemicals used. Follow local regulations and instructions for safe operation

and maintenance of the system.

WARNING!

There must always be a sample loop connected to ports 2 and 6

of the injection valve. This is to prevent liquid spraying out of the ports when

switching the valve. This is especially dangerous if hazardous chemicals are

used.

WARNING!

This is a Class A product. In domestic environment this product

may cause radio interference in which the user may be required to take

adequate measures.

Installation 2

Monitor UV-900 User Manual 18-1120-05 Edition AF

2 Installation

2.1 Unpacking

Unpack the instrument and check the items against the supplied packing list.

Inspect the items for obvious damage which may have occurred during

transportation

• The system should be installed on a stable laboratory bench providing a

suitable working area.

• To maintain correct ventilation, the system requires an appropriate

amount of free space. Do not block the ventilation inlets or outlets on the

system!

It is recommended that all packing materials should be retained if onward

transport of the instrument is expected.

2.2 General precautions

The instrument should not be installed in a corrosive atmosphere or in an

atmosphere where deposits are liable to form on the optical surfaces.

The instrument should be located in a place of low temperature variations,

away from heat sources, draughts and direct sunlight.

The instrument may be operated at normal ambient temperatures in the range

+4 to +40 °C.

The instrument should be installed on a stable laboratory bench or in

ÄKTA

™

explorer or ÄKTApurifier. To ensure correct ventilation a free space of

0.1 m is required behind and in front of the instrument. Place the instrument

directly on the bench. Do not use any soft material under the instrument, to

ensure that the ventilation inlet in the front is not blocked.

CAUTION!

The following information should be read carefully to ensure that

the instrument is installed correctly.

Monitor UV-900

2Installation

Monitor UV-900 User Manual 18-1120-05 Edition AF

2.3 Installing the flow cell

2.3.1 Fixing the flow cell

Handle the optical fibers with care,

do not bend excessively.

Two flow cells are available: UV-900/2 with 2 mm light path length

(cell volume 2 µl) and UV-900/10 with 10 mm path length (8 µl).

1 Unpack the flow cell. Do not remove the red protective caps from the inlet

or outlet, or the black protective caps on the optical fiber connectors.

2 Slide the rear white clip on the cell holder to its inner position for a 2 mm

cell and to its outer position for the 10 mm cell.

3 Place the flow cell in the opening between the white clips. The cell should

be positioned with the text and serial number facing upwards and to the

front. Press the cell into the clips to fasten it.

10 mm 2 mm

CAUTION!

To avoid damaging the optical fibers, press only on the cell body,

never on the optical fibers.

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