Lifecanvas Technologies SmartBatch+ Bedienungsanleitung
SmartBatch+
User’s Manual v1.11
1
Contents
Components & Accessories 2
Equipment Ratings 4
Installation 5
User Interface 9
Graphing & History 11
Operation 12
Clearing Mode 12
Labeling Mode 13
Switching Between Modes 14
Device Washing 14
Temperature Calibration 14
Replacing Electrodes 15
Replacing Fuse 15
Troubleshooting 16
Warranty 17
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2
Components & Accessories
The SmartBatch+ is a single device that electrophoretically delipidates and immunolabels
biological samples. During processing the samples are loaded into the Sample Chamber, where
an electric field is formed between the Electrodes to drive charged molecules into the samples.
For each operation, unique buffers are poured into the Sample Chamber (and drained out using
the Drain Valve). Finally, electrophoresis can only be applied once the Case Lid is closed.
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3
SmartBatch+ comes with the following accessories:
●1 x Clearing Cup (A)
●1 x Single Sample Staining Cup (B)
●1 x Medium Staining Cup (C)
●Power cord (D)
●1 x Batch Staining Cup (E)
●2 x Incubation Jar (F)
●1 x Extended Drain Tube (G)
●2 x Ring Stand (H)
●2 x Sample Ring and 2 x Lock Ring (I)
●2 x Extra Fuses (J)
●2 x Mesh Bag Set (K) - 2 x 12 bags for mouse brain size samples
●2 x Large Mesh Bag Set (L) - 2 x 4 bags for rat brain size samples
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Equipment Ratings
General Data:
Temperature Control
20-50°C
Electrophoresis Voltage
0-90 VDC
Electrophoresis Current Limit
0-1500 mA
Sample Specimen Size
30mm x 30mm x 20mm
Sample Specimen Capacity
Up to 12 mouse brains or 4 rat brains
Ambient Conditions:
Operating Temperature Range
18-26°C
Relative Humidity
80% Max
Storage Temperature
18-26°C
Storage Humidity
80% Max
Altitude
< 2,000 m
Electrical Data:
Rated Voltage Range (± 10%)
100-240 VAC
Rated Frequency (± 10%):
50-60 Hz
Power Consumption:
500W Max
Power Fuses:
T 10A 250V
Pollution Degree:
2
Electrical Overload Protection:
Yes
Dimensions:
L x W x H
250mm x 450mm x 350mm
Weight
~ 40 lbs
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5
Installation
Please take note of the following before installation:
●Do not use the instrument for purposes other than those indicated by the manufacturer.
●At least two people are required to move the instrument.
●The instrument must be placed in a stable and level location on a non-metal surface.
●The instrument must be placed such that the power cord and switch are easily
accessible at any time.
●Follow instructions contained in the manual when setting up and operating the
instrument, and when changing consumables.
●If deemed necessary, ask the manufacturer or your sales agent for help when setting up
the system.
●Connect the instrument to an electrical outlet according to local or country standard.
●Do not switch on the instrument without having waited at least 10 seconds after
switching it off.
●Only use buffers, sample cups, and parameter ranges provided by LifeCanvas
Technologies. See product warranty for further information.
●Use the volumes recommended by LifeCanvas Technologies.
●Do not leave the instrument in humid or wet conditions, as doing so could cause an
electrical short.
●Do not use the instrument outdoors.
●Leave at least 1 foot (30 cm) of space on the left and right sides of the device to allow for
proper airflow.
●Power requirements: 100-240 VAC, 50-60Hz, 5A
Note: In the event of unexpected problems, please contact your service representative or
LifeCanvas Technologies immediately:
info@lifecanvastech.com
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6
1. Carefully remove the device from the box, using one hand to grab the front of the device
and another hand to grab the back.
2. Remove the foam side panels and place the device down on a stable, level surface with
at least one foot of space (~30cm) on each side to allow for proper airflow.
Image:
3. Plug the power cord into the power entry module on the back of the device and then
plug it into the appropriate wall outlet.
4. Flip the power switch to the On position. The device will startup and the screen will turn
on.
5. Ensure that the drainage tube is in the closed position (the valve handle is perpendicular
to the tube as in image below):
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6. Remove the paper towels from the chamber and dispose of them. These are only here
to soak up any remaining liquid during shipping.
7. Pour ~400 mL distilled water directly into the Chamber.
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8. Ensure that there is water in the device and do not run the pump dry. Press “Auxiliary
Power” on the touchscreen. This will turn on the liquid pump, sample cup mixing, and
sample cup rotation. Upon startup the Chamber Hex Piece will rotate faster than normal
for 5 seconds to allow you to visually verify that the Hex Piece is indeed rotating. The
software will also deactivate so that power cannot be cycled too quickly for some
components.
9. If you wish to use the optional Extended Drain Tube, simply press the hard plastic end
into the bottom of the Drain Valve.
10. The device is now fully installed and you are ready to proceed!
11. If you will not be using the device immediately, turn off the Auxiliary Power, main power
switch and drain out the liquid in the chamber using the Drainage Valve.
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9
User Interface
The screen displays set values and present readings for various parameters of device
functionality. From this screen, the user can view and has control of: Voltage,Current Limit,
Timer Settings, and Temperature. The user can change between preset values for clearing and
labeling by pressing the “Preset” button. A display to the right of that button will indicate the
preset setting. To customize any of these settings, the user can enter a number into the main
keypad and press the “Set” button in the region of interest. Be aware of the number of decimal
places when changing these values for temperature, and the hours : minutes template for
changing the timer.
Complete description of UI used to control SmartBatch+ operation:
This is the Temperature panel. The top number shows the current
temperature reading as measured by the IR sensor on the lid on
top of the chamber. This directly measures the temperature of the
surface of the liquid underneath it. The device displays a rolling
average of measured temperatures, so it will take several seconds
to display the correct value on startup.
The bottom number shows the Set Temperature of the system.
The “Set” button will change the Set Temperature to the value that
is currently displayed in the keypad. If an invalid temperature
setting is input, a sound will play and the value will not change.
Note: The device is only heated by the electrophoresis, so without
that turned on the temperature will not increase. If the
temperature exceeds 60°C, the electrophoresis power will turn off
to prevent overheating.
This is the Voltage panel. The top number indicates the voltage that
is presently being applied across the electrodes. The bottom
number is the Set Voltage. The device normally operates in voltage
set, current limit mode. So, it will apply the Set Voltage unless the
current exceeds the Current Limit. If that is the case the Voltage
will decrease such that the Current Limit is not exceeded. To
change the Set Voltage, enter in the desired value in the keypad
and then press the “Set” button. If an invalid Set Voltage is input, a
sound will play and the value will go to the maximum allowable
value.
Note: The amount of heat generated is dependent on the power of
the electrophoresis. Exceeding the recommended values can
increase the thermal load too much and lead to overheating.
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