PolyScience Histology Bath Bedienungsanleitung

Operator’s Manual

Histology Bath

110-827

09.12.11

110-827 1

Table of Contents

Introduction............................................................................................................................................. 2

General Safety Information................................................................................................................................ 2

Safety Recommendations................................................................................................................................... 3

Regulatory Compliance & Testing..................................................................................................................... 3

Unpacking Your Histology Bath ........................................................................................................................ 4

Contents ................................................................................................................................................................. 4

Components & Controls ......................................................................................................................... 5

Installation .............................................................................................................................................. 6

General Site Requirements ................................................................................................................................ 6

Adding Liquid to the Reservoir.......................................................................................................................... 6

Electrical Power.................................................................................................................................................... 6

Normal Operation ....................................................................................................................................7

Routine Maintenance & Troubleshooting ............................................................................................ 8

Draining the Reservoir ........................................................................................................................................ 8

Cleaning the Bath .................................................................................................................................................8

Air Vents................................................................................................................................................................. 8

Air Filter ................................................................................................................................................................. 8

Troubleshooting Chart......................................................................................................................................... 9

Technical Information ............................................................................................................................ 9

Performance Specifications ............................................................................................................................... 9

Replacement Parts................................................................................................................................ 10

Equipment Disposal (WEEE Directive) ................................................................................................ 10

Service and Technical Support ............................................................................................................ 10

Warranty.................................................................................................................................................. 11

110-827 2

Introduction

Thank you for choosing this Histology Bath. Its low working temperature (-60°C / -76°F) freezes

samples quickly, preventing microscopic ice crystals from developing and samples from distorting. It

is ideal for fast-freezing tissues for enzyme studies. The Histology Bath is not a controlled cooling

source; it is designed to run at maximum cooling.

Standard features include:

• Continuous cooling to -60°C / -76°F

• 1.9 liter stainless steel reservoir and reservoir cover

• Immersion basket and chuck holder

• Front-mounted reservoir drain

• DuraTop™ insulating top deck

General Safety Information

When installed, operated, and maintained according to the directions in this manual and common

safety procedures, your Histology Bath should provide safe operation. Please ensure that all

individuals involved in the installation, operation, or maintenance of this Histology Bath read this

manual thoroughly prior to working with the unit.

This symbol marks chapters and sections of this instruction manual that are particularly

relevant to safety. When attached to the unit, this symbol draws attention to the relevant

section of the instruction manual.

This symbol indicates that hazardous voltages may be present.

This symbol marks information that is particularly important.

This symbol indicates alternating current.

This symbol on the Power Switch / Circuit Breaker indicates that it places the unit into a fully

powered state.

This symbol on the Power Switch / Circuit Breaker indicates that it disconnects power to the

unit.

This symbol indicates a protective conductor terminal.

Read all instructions pertaining to safety, set-up, and operation.

Proper operation is the user’s responsibility.

110-827 3

Safety Recommendations

To prevent injury to personnel and/or damage to property, always follow your workplace’s safety

procedures when operating this equipment. You should also comply with the following safety

recommendations:

WARNING: The Histology Bath uses isopentane as the bath fluid. Due to the flammability of

this liquid, always use the Histology Bath under a fume hood.

WARNING:

• Always connect the power cord on this Histology Bath to a grounded (3-prong) power outlet.

Make certain that the outlet is the same voltage and frequency as your unit.

• Never operate the Histology Bath with a damaged power cord.

• Always turn the Histology Bath Off and disconnect mains power before performing any

maintenance or service.

WARNING:

• Never operate the Histology Bath without bath fluid in the reservoir. Periodically check the

reservoir to ensure that the liquid depth is within acceptable levels. Always refill the

reservoir using the same bath fluid type that is already in the reservoir.

• Use compatible bath fluids only.

• Always drain all fluid from the reservoir before moving or lifting your Histology Bath. Be

sure to follow your organization’s procedures and practices regarding the safe lifting and

relocation of heavy objects.

WARNING: Always allow the bath fluid to return to ambient temperature before draining.

WARNING: It is the user’s responsibility to properly decontaminate the unit in the event

hazardous materials are spilled on exterior or interior surfaces. Consult manufacturer if there

is any doubt regarding the compatibility of decontamination or cleaning agents.

Regulatory Compliance & Testing

EC Low Voltage Directive 2006/95/EC

EC Electromagnetic Compatibility Directive 2004/108/EC

IEC 61010-1-2001

IEC 61010-2-2001

IEC 61326:2005 / EN 61326 : 2006

110-827 4

Unpacking Your Histology Bath

Your Histology Bath is shipped in a special carton. Retain the carton and all packing materials until

the unit is completely assembled and working properly. Set up and run the unit immediately to

confirm proper operation. Beyond one week, your unit may be warranty repaired, but not replaced.

If the unit is damaged or does not operate properly, contact the transportation company, file a

damage claim, then contact the company where your unit was purchased.

WARNING: Keep unit upright when moving. Be sure to follow your company’s procedures and

practices regarding the safe lifting and relocation of heavy objects.

Contents

The items included with your Histology Bath are:

• Histology Bath

• Reservoir Cover

• Immersion Basket and Chuck Holder

• Power Cord

• Operator’s Manual

110-827 5

Components & Controls

Power Switch / Circuit Breaker

Reservoir Cover

Drain Valve

Drain Port

IEC Power

Connection

Reusable Air Filter

(behind grille)

110-827 6

Installation

Your Histology Bath is simple to setup and install. The only tool required is a container for adding a

suitable fluid to the bath reservoir.

General Site Requirements

WARNING: The Histology Bath uses isopentane as the bath fluid. Due to the flammability of

this liquid, always use the Histology Bath under a fume hood.

Locate your Histology Bath on a level surface in an area that is free from drafts and wide ambient

temperature variations, such as near heater or air conditioning vents. Do not place it where there

are corrosive fumes, excessive moisture, or in excessively dusty areas.

Position the unit to allow for unobstructed air flow through the side and rear vents. A space of at

least 4 inches (10.2 cm) should be provided behind the unit.

Avoid voltage drops by using properly grounded power outlets wired with 14 gauge or larger

diameter wire and if possible, be close to the power distribution panel. The use of extension cords is

not recommended; this will reduce the potential for problems caused by low line voltage.

Adding Liquid to the Bath Reservoir

WARNING: Fill reservoir with fluid before applying power to the unit.

WARNING: Read the safety data sheet for the bath fluid being used carefully before filling

reservoir.

The Histology Bath is designed for use with isopentane (also known as 2-methlybutane).

Fill bath so that the liquid level is approximately 1 inch (2.54 cm) from the top for best refrigeration

performance. Be sure to allow for the fluid displacement that will result when the immersion basket

and samples are placed in the reservoir.

WARNING: Always drain all fluid from the reservoir before moving or lifting your Histology

Bath. Be sure to follow your organization’s procedures and practices regarding the safe lifting

and relocation of heavy objects.

WARNING: To avoid the potential for burns, allow the Histology Bath to return to ambient

temperature before cleaning or performing any maintenance.

Electrical Power

WARNING: The Histology Bath’s power cord must be connected to a properly grounded

electrical receptacle. Make certain that this electrical outlet is the same voltage and frequency

as your unit. The correct voltage and frequency for your Histology Bath are indicated on the

identification label on the rear of the unit.

CAUTION: The use of an extension cord is not recommended. If one is necessary, it must be

properly grounded and capable of handling the total wattage of the unit. The extension cord

must not cause more than a 10% drop in voltage to the unit.

Plug the Histology Bath’s power cord into a properly grounded electrical outlet of the correct

voltage and frequency.

110-827 7

Normal Operation

WARNING: Due to the flammable nature of isopentane, the Histology Bath should always be

used under a fume hood. Be sure to wear appropriate protective equipment, such as splash

goggles and gloves.

NOTE: The Histology Bath operates at maximum cooling performance.

Place the Power Switch / Circuit Breaker on the front of the Histology Bath in the On position. The

compressor will start up and the unit will begin cooling. It will take about one hour for the unit to

achieve a -60°C / -76°F working temperature.

Once the unit has reached working temperature, remove the reservoir cover and slowly submerge

the immersion basket in the reservoir. Use the tissue mounts (not supplied) and chuck holder to

position the samples properly in the sample basket. Replace the reservoir cover.

Immersion Basket

Tissue Mounts

(user-supplied)

Chuck Holder

110-827 8

Routine Maintenance & Troubleshooting

WARNING: Hazardous voltages may be present. Disconnect power before performing

maintenance.

Draining the Reservoir

To drain fluid from the bath, attach a short length of suitable 11.5 mm ID / 0.45 inch ID tubing to the

drain port and secure it using a hose clamp with a minimum ID of 18 mm / 0.7 inch. Open the drain

valve using a flat blade screwdriver. When closing the valve, do not over tighten.

WARNING: Be sure to close the drain valve before refilling the bath reservoir. Do not over

tighten.

Cleaning the Bath

Thoroughly clean the bath before each use. Use only mild soap and water when cleaning. Do not use

steel wool as damage to the unit may result. Non-steel scouring pads are acceptable.

The entire unit is housed in a tough, well-insulated stainless steel casing that is corrosion and

chemical resistant.

Air Vents

The unit draws air through the rear and exhausts it through the side vents. The vents should be

inspected regularly for the accumulation of dust and cleaned as required.

Air Filter

To keep the refrigeration system operating at optimum cooling capacity, the reusable filter located

at the rear of the unit should be kept free of dust and dirt. It should be checked on a scheduled basis

and cleaned as required.

To access the air filter, remove the three screws securing the grille cover to the unit’s housing,

detach the grille, and then gently remove the air filter from the filter compartment. Use a mild

detergent and water solution to wash off any accumulated dust and dirt and then rinse and dry

thoroughly before reinstalling.

Grille

Air Filter

110-827 9

Troubleshooting Chart

Problem Cause Corrective Action

No power to unit Electrical power

disconnected

Power Switch / Circuit

Breaker in Off position

Compressor overload switch

has tripped

Check that electrical cord is secure and plugged

into an operating electrical outlet.

Place Power Switch / Circuit Breaker in On

position.

Allow approximately 10 minutes for unit to restart.

No or insufficient

cooling

Blocked air flow

High ambient temperature

Improper line voltage and/or

frequency

Check air vents for blockage.

Check that air flow around unit is not restricted

and that room temperature does not exceed 95°F

/ 35°C.

Verify that Mains voltage during start cycle is

within 10% of rated voltage.

Gradual loss of cooling Blocked air flow Check air vents for blockage.

Compressor shakes,

stalls, or continually

restarts

Compressor cycling or

overload

Check air vents for blockage.

Check that air flow around unit is not restricted

and that room temperature does not exceed 95°F

/ 35°C.

Verify that Mains voltage during start cycle is

within 10% of rated voltage.

Check compressor fan for operation (listen for fan

noise, check airflow through unit).

Technical Information

Performance Specifications

Working Temperature: Fixed at -60°C / -76°F

Reservoir Capacity: 1.9 liters

Tank: Insulated stainless steel

Bath Work Area: 4.8 ∅x 6.6 inches deep / 12.2 ∅x 16.8 cm deep

Overall Dimensions (L x W x H): 22.75 x 11 x 11 inches / 57.8 x 27.9 x 27.9 cm

Electrical Requirements: 60 Hz: 120V, 60Hz, 5.0A

50 Hz: 240V, 50Hz, 2.5A

Specifications subject to change without notice.

Environmental Conditions Indoor use only

Maximum Altitude: 2000 meter

Operating Ambient: 5° to 35°C (41° to 95°F)

Relative Humidity: 80%, non-condensing

Installation Category: II

Pollution Degree: 2

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