Qiagen QIAcube Bedienungsanleitung

Version 1.1 June 2008

Sample & Assay Technologies

QIAcube®User Manual

Trademarks: QIAGEN®, QIAamp®, QIAcube®, QIAprep®, QIAquick®, DNeasy®, EpiTect™, MinElute®, RNeasy®(QIAGEN Group); DNA-ExitusPlus™

(AppliChem); Gigasept®, Lysetol®, Mikrozid®(Schülke & Mayr GmbH); DECON-QUAT®(Veltek Associates, Inc.); RNaseZap® (Ambion, Inc.).

Contents

QIAcube User Manual 06/2008 Contents-1

Contents

1Safety Information 1-1

1.1 Proper use 1-1

1.2 Electrical safety 1-2

1.3 Environment 1-3

1.4 Biological safety 1-4

1.5 Chemicals 1-6

1.6 Waste disposal 1-7

1.7 Mechanical hazards 1-7

1.8 Heat hazard 1-8

1.9 Translations of warnings and cautions 1-8

1.10 Symbols on the QIAcube 1-26

2Introduction 2-1

2.1 About this user manual 2-1

2.2 General information 2-2

2.2.1 Technical assistance 2-2

2.2.2 Policy statement 2-2

2.2.3 Version management 2-2

2.3 Intended use of the QIAcube 2-3

2.3.1 Requirements for QIAcube users 2-3

3General Description 3-1

3.1 QIAcube principle 3-2

3.2 External features of the QIAcube 3-4

3.3 Internal features of the QIAcube 3-7

Contents

Contents-2 QIAcube User Manual 06/2008

4Installation Procedures 4-1

4.1 Requirements 4-1

4.2 AC power connection 4-2

4.3 Unpacking the QIAcube 4-2

4.4 Installing the QIAcube 4-4

4.5 Packing the QIAcube 4-7

5Operating Procedures 5-1

5.1 QIAcube software 5-2

5.2 Running a QIAGEN protocol 5-2

5.2.1 Starting a protocol 5-3

5.2.2 End of the protocol run 5-9

5.2.3 Stopping a protocol 5-9

5.3 Setting up the worktable 5-9

5.3.1 Loading the reagent bottle rack 5-9

5.3.2 Loading the tip racks 5-11

5.3.3 Loading the rotor adapters 5-12

5.3.4 Loading the samples 5-16

5.3.5 Loading accessory buffers 5-18

5.4 Installing and deleting protocols 5-20

5.5 Saving data files 5-23

5.6 General information 5-26

5.7 Operating the shaker 5-29

5.8 Operating the centrifuge 5-32

6Maintenance Procedures 6-1

6.1 Regular maintenance procedure 6-4

6.2 Daily maintenance procedure 6-4

6.3 Monthly maintenance procedure 6-5

6.4 Periodic maintenance procedure 6-6

Contents

QIAcube User Manual 06/2008 Contents-3

6.4.1 Cleaning the centrifuge 6-6

6.4.2 Cleaning the instrument 6-10

6.4.3 Tightness test 6-10

6.5 Decontaminating the QIAcube 6-11

6.6 Cleaning the QIAcube centrifuge after a plastic crash 6-12

6.6.1 Opening the centrifuge lid 6-12

6.6.2 Cleaning the rotor and buckets 6-12

6.6.3 Cleaning the centrifuge 6-13

6.6.4 Cleaning the worktable 6-15

6.6.5 Cleaning the gripper unit 6-16

6.6.6 Installing the centrifuge rotor and buckets 6-17

6.6.7 Operating the centrifuge after cleaning 6-18

7Troubleshooting 7-1

7.1 General information 7-1

7.2 Resolving error messages and warnings 7-1

7.2.1 Load check errors 7-2

7.2.2 Protocol errors 7-4

7.2.3 File system errors 7-7

7.2.4 General instrument errors 7-8

7.2.5 General troubleshooting 7-14

7.2.6 Protocol interruption 7-14

7.2.7 Centrifuge 7-15

7.2.8 Shaker 7-20

7.2.9 Touchscreen 7-20

7.2.10 USB stick 7-20

7.2.11 Reagent volume detection 7-21

Contents

Contents-4 QIAcube User Manual 06/2008

8Glossary 8-1

Appendix A A-1

Technical data A-1

Environmental conditions A-1

Waste Electrical and Electronic Equipment (WEEE) A-3

FCC declaration A-4

EC declaration of conformity A-6

Appendix B B-1

Loading the centrifuge and shaker B-1

Appendix C C-1

QIAcube accessories C-1

Appendix D D-1

Liability clause D-1

Index Index-1

Safety Information

QIAcube User Manual 06/2008 1-1

1Safety Information

Before using the QIAcube, it is essential that you read this

user manual carefully and pay particular attention to the

safety information. The instructions and safety information in

the user manual must be followed to ensure safe operation

of the instrument and to maintain the instrument in a safe

condition.

The following types of safety information appear throughout

this manual.

WARNING

The term WARNING is used to inform you about situations

that could result in personal injury to you or other

persons.

Details about these circumstances are given in a box like

this one.

CAUTION

The term CAUTION is used to inform you about situations

that could result in damage to the instrument or other

equipment.

Details about these circumstances are given in a box like

this one.

The advice given in this manual is intended to supplement,

not supersede, the normal safety requirements prevailing in

the user’s country.

1.1 Proper use

WARNING

Risk of personal injury and material damage [W1]

Improper use of the QIAcube may cause personal injuries

or damage to the instrument.

The QIAcube must only be operated by qualified personnel

who have been appropriately trained.

Servicing of the QIAcube must only be performed by

QIAGEN Field Service Specialists.

Perform the maintenance as described in Section 6. QIAGEN

charges for repairs that are required due to incorrect

maintenance.

Safety Information

1-2 QIAcube User Manual 06/2008

WARNING

Risk of personal injury and material damage [W2]

The QIAcube is too heavy to be lifted by one person. To

avoid personal injury or damage to the instrument, do not

lift the instrument alone.

WARNING

Risk of personal injury and material damage [W3]

Do not attempt to move the QIAcube during operation.

CAUTION

Damage to the instrument [C1]

Avoid spilling water or chemicals onto the QIAcube.

Damage caused by water or chemical spillage will void

your warranty.

In case of emergency, switch off the QIAcube at the power

switch at the front of the instrument and unplug the power

cord from the power outlet.

CAUTION

Damage to the instrument [C2]

Only use QIAGEN®spin columns and QIAGEN Spin Kits

with the QIAcube. Damage caused by use of other types of

spin column or chemistries will void your warranty.

1.2 Electrical safety

Disconnect the line power cord from the power outlet before

servicing.

Safety Information

QIAcube User Manual 06/2008 1-3

WARNING

Electrical hazard [W4]

Any interruption of the protective conductor (earth/ground

lead) inside or outside the instrument or disconnection of

the protective conductor terminal is likely to make the

instrument dangerous.

Intentional interruption is prohibited.

Lethal voltages inside the instrument

When the instrument is connected to line power, terminals

may be live, and opening covers or removing parts is likely

to expose live parts.

To ensure satisfactory and safe operation of the QIAcube,

follow the advice below:

The line power cord must be connected to a line power

outlet that has a protective conductor (earth/ground).

Do not adjust or replace internal parts of the instrument.

Do not operate the instrument with any covers or parts

removed.

If liquid has spilled inside the instrument, switch off the

instrument, disconnect it from the power outlet, and

contact QIAGEN Technical Services.

If the instrument becomes electrically unsafe, prevent other

personnel from operating it, and contact QIAGEN Technical

Services; the instrument may be electrically unsafe when:

It or the line power cord appears to be damaged.

It has been stored under unfavorable conditions for a

prolonged period.

It has been subjected to severe transport stresses.

1.3 Environment

Operating conditions

WARNING

Explosive atmosphere [W5]

The QIAcube is not designed for use in an explosive

atmosphere.

Safety Information

1-4 QIAcube User Manual 06/2008

WARNING

Risk of explosion [W6]

The QIAcube is intended for use with reagents and

substances supplied with QIAGEN kits. Use of other

reagents and substances may lead to fire or explosion.

CAUTION

Damage to the instrument [C3]

Direct sunlight may bleach parts of the instrument and

cause damage to plastic parts.

The QIAcube must be located out of direct sunlight.

1.4 Biological safety

Specimens and reagents containing materials from humans

should be treated as potentially infectious. Use safe

laboratory procedures as outlined in publications such as

Biosafety in Microbiological and Biomedical Laboratories,

HHS ( www.cdc.gov/od/ohs/biosfty/biosfty.htm ).

Samples

Samples may contain infectious agents. You should be aware

of the health hazard presented by such agents and should

use, store, and dispose of such samples according to the

required safety regulations.

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