VWR VisiScope 384 Series Bedienungsanleitung

Model European Catalogue Number

BL384 P 630-1930

TL384 P 630-1931

BL384 PI 630-1932

TL384 PI 630-1933

BL384 PH 630-1934

TL384 PH 630-1935

BL384 PHI 630-2529

TL384 PHI 630-2530

INSTRUCTION MANUAL

Version: 7

Issued: 20, 10, 2014

VWR

Microscope series 384

Table of Contents

Warning

Safety information

Package contents

Unpacking

Intended use

Symbols and conventions

Product specications

Overview

Automatic Light Control (BL384 P and BL384 PI models)

Operation

Observation head

Place the specimen on the stage

Illumination system settings

Adjust interpupillary distance

Focus tension adjustment

Diopter adjustment

Condenser

Numerical aperture setting

Phase rings centering (models -PH)

Troubleshooting

Repair and maintenance

User replaceable accessories and spare parts

Technical service

Warranty

Compliance with local laws and regulations

Disposal

Legal Address of Manufacturer

Country of origin: ITALY

Europe

VWR International bvba

Researchpark Haasrode 2020

Geldenaaksebaan 464

B-3001 Leuven

+ 32 16 385011

http://be.vwr.com

Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of main-

tenance. It is built to high optical and mechanical standards and to withstand daily use.

We remind you that this manual contains important information on safety and maintenance, and that it must

therefore be made accessible to the instrument users.

We decline any responsibility deriving from incorrect instrument use uses that does not comply with this

manual.

Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position.

Users should observe all safety regulations of the region. The equipment has acquired the CE safety label.

However, users have full responsibility to use this equipment safely.

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Package Contents

DESCRIPTION QUANTITY

Microscope stand with nosepiece, stage, condenser 1

Optical head ( BL: binocular; TL: trinocular) 1

Objective 4x 1

Objective 10x 1

Objective 40x 1

Objective 100x 1

Eyepiece WF10x/20mm 2

Immersion oil 1

Dust cover 1

Power supply output 6Vdc 1

Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the contain-

er and lift the top half of the container. Take care to ensure that the optical items (objectives and eyepieces)

do not fall out and get damaged. Using both hands (one around the neck and one around the base), lift the

microscope from the container and put it on a stable desk.

Place the observation head onto the top of the neck and tighten the lock-screw. Insert the eyepieces into the

eye tubes.

Connect the provided power supply to the power supply input jack on the rear of the microscope.

Intended use

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with

caution

Product specications

Model Head Eyepiece

Nosepiece

Objectives Stage Focusing Illuminator Condenser Power supply

TL384 P Trinocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple,

reversed 160mm

E-PLAN

4X/0.10,

10X/0.25,

40X/0.65,

100X/1.25

Double layer

160x140mm,

X-Y 76x52mm

moving range.

Coaxial coarse

and ne

focusing

P-LED system

with adjustable

intensity

Abbe con-

denser, sliding-

in, N.A. 1.25

with centering

system.

External power

supply:

Input 100-

240Vac 50-60Hz

/ Output 6Vdc

BL384 P Binocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple,

reversed 160mm

E-PLAN

4X/0.10,

10X/0.25,

40X/0.65,

100X/1.25

Double layer

160x140mm,

X-Y 76x52mm

moving range.

Coaxial coarse

and ne

focusing

P-LED system

with adjustable

intensity.

Automatic bright-

ness control.

Abbe con-

denser, sliding-

in, N.A. 1.25

with centering

system.

External power

supply:

Input 100-

240Vac 50-60Hz

/ Output 6Vdc

TL384 PI Trinocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple,

reversed IOS

E-PLAN

4X/0.10,

10X/0.25,

40X/0.65,

100X/1.25

Double layer

216x150mm,

X-Y 78x54mm

moving range.

X belt-drive.

Coaxial coarse

and ne

focusing

P-LED system

with adjustable

intensity

Abbe con-

denser, sliding-

in, N.A. 1.25

with centering

system.

External power

supply:

Input 100-

240Vac 50-60Hz

/ Output 6Vdc

BL384 PI Binocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple,

reversed IOS

E-PLAN

4X/0.10,

10X/0.25,

40X/0.65,

100X/1.25

Double layer

216x150mm,

X-Y 78x54mm

moving range.

X belt-drive.

Coaxial coarse

and ne

focusing

P-LED system

with adjustable

intensity.

Automatic bright-

ness control.

Abbe con-

denser, sliding-

in, N.A. 1.25

with centering

system.

External power

supply:

Input 100-

240Vac 50-60Hz

/ Output 6Vdc

BL384 PH

Binocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple,

reversed IOS

E-PLAN

4X/0.10,

10XPH/0.25,

40XPH/0.65,

100XPH/1.25

Double layer

160x140mm,

X-Y 76x52mm

moving range.

Coaxial coarse

and ne

focusing

P-LED system

with adjustable

intensity.

Automatic bright-

ness control.

Phase condens-

er (10x,/20x

40x, 100x)

with darkeld

(dry) and

brighteld

External power

supply:

Input 100-

240Vac 50-60Hz

/ Output 6Vdc

TL384 PH

Trinocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple,

reversed IOS

E-PLAN

4X/0.10,

10XPH/0.25,

40XPH/0.65,

100XPH/1.25

Double layer

160x140mm,

X-Y 76x52mm

moving range.

Coaxial coarse

and ne

focusing

P-LED system

with adjustable

intensity

Phase condens-

er (10x,/20x

40x, 100x)

with darkeld

(dry) and

brighteld

External power

supply:

Input 100-

240Vac 50-60Hz

/ Output 6Vdc

BL384 PHI

Binocular,

360° rotat-

ing,

30° inclined

Wideeld,

10x/20mm Quintuple

nosepiece,

reversed

IOS PLAN

10xPh,

20xPh,

40xPh,

00xPh (oil)

Double layer

mechanical

sliding stage,

216x150mm,

moving range

78x54mm,

Belt-drive in X

direction

Coaxial

coarse and

ne focusing

with

limit stop

P-LED, with

Automatic

Light Control

Phase conden-

ser

(10x/20x, 40x,

100x)

with darkeld

(dry) and

brighteld

External power

supply:

Input 100-

240Vac

50-60Hz.

Output 6Vdc 1A

TL384 PHI

Trinocular

360°rotating

30° inclined

Wide Field

10X/20

Quintuple

nosepiece,

reversed

PLAN

4x, 10xPh,

40xPh,

100xPh (oil)

Double layer

mechanical

sliding stage,

160x140mm,

moving range

78x54mm

Coaxial

coarse and

ne focusing

with

limit stop

P-LED, with

manual

brightness

control

Phase conden-

ser (10x/20x,

40x, 100x)

with darkeld

(dry) and

brighteld

External power

supply:

Input 100-

240Vac

50-60Hz.

Output 6Vdc 1A

PHOTO/VIDEO PORT

HEAD LOCKING

SCREW (1)

REVOLVING NOSEPIECE

STAGE

TRANSLATION KNOBS

BRIGHTNESS

ADJUSTMENT

(LEFT SIDE)

CONDENSER CENTERING

SCREWS (2)

IRIS DIAPHRAGM (3)

EYEPIECE

CONDENSER

LED ILLUMINATOR

OBJECTIVE

DIOPTRIC

ADJUSTMENT RING

Overview

SLIDE CLAMP

FINE FOCUSING KNOB

CONDENSER HEIGHT

ADJUSTMENT (4)

TENSION

ADJUSTMENT KNOB (5)

COARSE FOCUSING KNOB

FOCUS STOP KNOB

INTERPUPILLARY DISTANCE

Automatic Light Control (BL384 P, BL384 PI and BL384 PH models)

BL384 P and BL384 PI models have a special function called “Automatic Light Control”.

The level of light is adjusted by the microscope in order to maintain the same level as the one the user has

chosen, no matter if the aperture of the diaphragm changes, another objective is inserted, opacity of the

sample changes, etc.

1) Set the focus on the sample as described in the previous chapters, using the objective of your choice.

2) Rotate the brightness adjustment knob in order to get a comfortable level of illumination.

NOTE: ALC system works properly with medium-to-high light intensity, do not set the brightness to a

minimum level.

3) Press the ALC button on the side of the microscope.

Now the ALC system is working: if you open or close the aperture diaphragm or change objective, the system

will try to maintain the same level of illumination as the one you stored when you pressed the button.

4) Press the ALC button again to return to a manual control of illumination.

NOTE: ALC system is not suitable for use with phase contrast.

BRIGHTNESS

ADJUSTMENT

KNOB

ALC BUTTON

Operation

Observation head

Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the

lock-screw.

Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide holder. Ensure that the specimen is centred

over the stage opening by adjusting the coaxial stage controls.

Illumination system settings

The microscope is tted with a white LED illuminator. Before turning on the illumination system, read the

section about electrical safety precautions. Insert the plug of the cable into the power socket and turn on the

switch on the back of the main body. Turn the brightness adjustment knob to a brightness suitable for obser-

vation.

Adjust interpupillary distance

Hold the right and left side of the observation head with both hands and adjust the interpupillary distance by

turning the two parts until one circle of light can be seen, when looking down the microscope with both eyes.

The white dot (°) placed on the right eyepiece shows the set interpupillary distance. Just remember this value

to help on later settings.

Focus tension adjustment

The tension of the coarse focusing knob is preset by factory. To change the tension according to your prefer-

ence, just rotate the knob clockwise in order to increase it.

Excessive tension could damage the mechanism of focus. A too loosed tension causes the descent of the

stage by gravity or a sudden loss of focus. In this case, rotate the knob to increase the tension.

Diopter adjustment

Turn the dioptric adjustment ring on the right eyepiece dioptric up to align the bottom with the graduated ring.

Turn the coarse focus knob in order to focus the slide with an objective with low magnication. Adjust the

ne focus knob until you obtain a clear and dened picture observing with the right eye, and then repeat the

operation with the left dioptric compensation ring and the left eye. When the image appears in focus, choose

the necessary objective with the revolving nosepiece.

Condenser

Raise or lower the condenser through the knob to obtain a clear and uniform illumination of the sample.

To center the condenser: completely close the iris diaphragm. Using the condenser centering screws, move

the diaphragm in the center of the eld of view. Then gradually expand the diaphragm until it is tangent to the

edges of the eld of view. If necessary, you can perform an additional adjustment.

The condenser is centered when the edges of iris diaphragm are tangent to the eld of view.

Numerical aperture setting

The value of the numerical aperture (N.A.) of the diaphragm is an indication of the contrast of the illumination

system. Matching the value of illumination system’s N.A. with that of the objective ensures the best results in

terms of contrast and image quality. To set the numerical aperture of the Illuminator, adjust the opening of the

iris diaphragm. In this way you control contrast and image resolution. For samples with low contrast set the

iris to about 75% of the value of the objective’s numerical aperture.

Phase rings centering (models -PH)

For models equipped with phase contrast set, you have to perform the centering of the phase rings.

Remove an eyepiece from the head and insert the centering telescope in the empty tube.

Insert the 10x objective rotating the nosepiece.

Rotate the turret of the condenser until you reach the inscription “10”.

Loosening the lock screw of the centering telescope, focus on the light ring that you observe.

Rotate the two centering screws in order to move the bright ring until it is perfectly aligned with the dark ring.

Repeat for the other objectives (only as a verication of the correct centering).

Phase rings will be centered when you see an image like this:

Troubleshooting

Review the information in the table below to troubleshoot operating problems.

PROBLEM CAUSE SOLUTION

LIGHT DOESN’T TURN ON Power supply not connected Check that the 6Vdc power supply

jack is well inserted on the rear of the

microscope

Rotate the brightness adjustment con-

trol and check if there is an increase in

the light output

IMAGE CANNOT BE SEEN OR

IS DARK.

The iris diaphragm aperture

is not completely opened

Completely open the iris diaphragm

aperture

Brightness level is low Rotate the brightness adjustment po-

tentiometer

Objective is not aligned with

the optical axis

Rotate the nosepiece until an objective

is well inserted in the optical path (it

“clicks”)

IMAGE IS UNCLEAR, BLURRED

OR HAS INSUFFICIENT CON-

TRAST.

Objectives or lters are dirty Wipe them clean

The iris diaphragm aperture

is not opened correctly

Open the iris diaphragm aperture

completely

Condenser at wrong height Rotate the condenser knob until you

see a uniform illumination

Repair and maintenance

Microscopy environment

This microscope is recommended for use in a clean, dry and shock free environment with a temperature of

0-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.

After using the microscope

The microscope should always be kept vertical when moving it so that no moving parts, such as the eye-

pieces, fall out.

Never mishandle or impose unnecessary force on the microscope. Never attempt to service the microscope

yourself. Turn down the illumination intensity control and turn the light off. Cover the microscope with the in-

cluded dust cover, and keep it in a dry and clean place.

Electrical safety precautions

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off-position.

Users should observe all safety regulations of the region. The equipment has acquired the CE safety label.

However, users do have full responsibility to use this equipment safely.

Cleaning the optics

If the optical parts require cleaning rst use compressed air.

If that is not sufcient use a soft lint-free cloth with water and a mild detergent.

And as a nal option use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.

Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or

near electric equipment. Use these chemicals in a well ventilated room.

Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the op-

tics. Do not disassemble objectives or eyepieces in an attempt to clean them.

For the best results, use the VWR cleaning kit (see catalogue number below).

If you need to send the microscope to manufacturer for maintenance, please use the packaging if possible.

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