Nikon ECLIPSE E200 POL Bedienungsanleitung

Microscope

ECLIPSE E200 POL

Instructions

M260E 04.2.CF.3(1/2)

Thank you for purchasing this Nikon product. This instruction manual is for the users of the Nikon Microscope ECLIPSE

E200 POL describing basic operation of the microscope.

To ensure correct usage, please read this manual thoroughly before using the microscope.

• It is prohibited to reproduce or transmit this manual in any form without the prior consent of Nikon.

• The instructions and specifications in this manual are subject to change without notice.

• Although every effort has been made to ensure the accuracy of this manual, if you find that any part of

this manual is unclear or incorrect, contact your nearest Nikon representative.

• Some ECLIPSE E200 POL microscope sets may come with different components and accessories from

those shown in this manual.

• Also read the manuals for the products used with the microscope, for example, the Nikon

photomicrographic equipment.

Safety Precautions

Warning and Caution Symbols Used in This Manual

Though Nikon products are designed to provide you with utmost safety during use, incorrect usage or disregard of the

instructions may cause personal injury or property damage and will lead to the forfeiture of all claims against warranty.

For your own safety, read the instruction manual carefully and thoroughly before using the product. Do not discard

this manual. Always keep it near the product for easy reference.

Inside this instruction manual, safety instructions are indicated with the symbols shown below. Be sure to follow the

instructions marked with these symbols for your safety.

Symbol Meaning

Disregarding instructions marked with this symbol may lead to death or serious injury.

Disregarding instructions marked with this symbol may lead to injury or property

damage.

Meaning of Symbols Used on the Equipment

The symbol appearing on the product indicates the need for caution at all times during use.

Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the

symbol has been affixed.

Symbol Meaning

Caution! Biohazard

This symbol label attached on the stand reminds you of the following:

• WARNING: Contact of a living body sample to the microscope, presents a biohazard risk.

• To avoid biohazard contamination, do not touch the contaminated portion with your bare

hands.

• Decontaminate the contaminated portion according to the standard procedure of your

laboratory.

Caution for heat

This symbol label attached near the field lens unit (the lamp is set underneath the field lens

unit) reminds you of the following:

• Lamp and its surrounding areas (including the field lens unit) become very hot during and

immediately after illumination.

• Risk of burns. Do not touch the lamp or surrounding areas during and immediately after

illumination.

• Make sure the lamp and its surrounding areas have cooled sufficiently before attempting to

replace the lamp.

Caution

This symbol label attached near the AC inlet reminds you of the following:

• Check the input voltage before turning on the microscope.

(The input voltage is given on the "nameplate" and above the AC inlet.)

• If the input voltage shown differs from the local voltage level, do not turn on the microscope.

Do the following instead:

Different voltage on the nameplate Contact your nearest Nikon representative.

Different voltage above the AC inlet Change the input voltage setting; refer to P.35.

WARNING

CAUTION

Safety Precautions

1. Intended use of the equipment

This microscope is intended mainly for use in microscopic observation of rock and mineral or polymer

materials or substance of living body, using polarized light illumination.

It is designed for the main purposes of analysis of the optical character, in laboratories or hospitals, of

such samples within the fields of mineralogy, high polymer chemistry, medicine.

2. Do not disassemble.

Disassembly may cause malfunction and/or electrical shock, and will lead to the forfeiture of all claims

against warranty. Do not disassemble any part other than those described in this manual. If you

experience any problem with the microscope, notify your nearest Nikon representative.

3. Check the input voltage.

The input voltage is indicated in two places at the rear of the microscope: on the nameplate and above

the AC inlet. Confirm that these input voltage indications correspond to the voltage provided in your

region. If not, follow one of the instructions below. The use of microscopes with the different input

voltage indications will cause overcurrent and overheating, which may result in fire or severe damage

to the microscope.

• If the voltage indication on the nameplate differs:

ÎDo not plug in the microscope. Contact your nearest

Nikon representative.

• If the voltage indication above the AC inlet differs:

ÎRefer to p. 35 and change the input voltage

setting before turning on the power switch.

• For the microscope with the nameplate showing

[100/110/120 V ~]:

The voltage can be set to: AC 100 V, 110 V or 120 V.

• For the microscope with the nameplate showing

[220/230/240 V ~]:

The voltage can be set to: AC 220 V, 230 V or 240 V.

4. Use the specified lamp, fuse, and power cord.

Use the specified lamp and fuse. Use the power cord provided. Using an incorrect lamp, fuse, or power

cord may damage the instrument or cause a fire. (Also see p. 50 on power cord.)

If using an extension cord, only use a cord that includes a protective earth (PE) wire.

• Specified Lamp

Halogen lamp 6 V-20 W (PHILIPS 7388 or OSRAM HLX64250) or

Halogen lamp 6 V-30 W (PHILIPS 5761)

• Specified Fuse

250 V, 1 A, time-lag low-breaking type, 5x20 miniature fuse x2

Input Voltage

Indications

WARNING

Safety Precautions

5. Heat at the light source

The lamp becomes hot during use. Do not remove the field lens unit while the lamp is on, and be sure

the lamp has been off for 30 minutes before touching it.

• When changing the lamp bulb, make sure that the lamp is cool enough to touch (the light should be

off at least 30 minutes).

• Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so could

result in burns.

• Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum benzine,

acetone, thinner, or alcohol near a hot lamp, as a fire could result.

6. Hazardous sample

This microscope is mainly for use in microscopic observation of rock and mineral or polymer materials

or substance of living body, using polarized light illumination.

When handling a living body sample, check to determine whether the sample is hazardous.

Handle hazardous samples according to the standard procedure of your laboratory.

If the sample is of an infectious nature, wear rubber gloves to avoid infection, and be careful not to

touch a sample. In the event of contact of a sample to the microscope, decontaminate the

contaminated portion according to the standard procedure of your laboratory.

1. Turn off power switch before assembling the microscope, replacing the lamp or fuse, and

plugging in or unplugging the power cord.

Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or fire.

Also turn off the power switch and then unplug the power cord before assembling the microscope, and

before changing the lamp or fuse. To turn off the power, turn the power switch to {.

2. Keep the microscope free of moisture and foreign matter.

Keep the microscope free of moisture to prevent short circuiting that could result in overheating or

other malfunctions. If water splashes on the microscope, immediately turn off the power switch (turn

the switch to {) and unplug the power cord. Then, wipe off the water with a dry cloth. Short circuiting

can also result when foreign matter is trapped inside the microscope. If foreign matter or water has

entered the microscope, do not use the microscope and contact your nearest Nikon representative.

3. Disposal of the microscope

To avoid biohazard risk, dispose of the microscope as the contaminated equipment according to the

standard procedure of your laboratory.

WARNING

CAUTION

Safety Precautions

Notes on Handling the System

（1）Installation

This microscope is a precision instrument. Using the microscope in an unfavorable environment

could result in malfunctions or degraded performance. Consider the following conditions when

choosing the installation location.

• Observation conditions are better if light from windows and bright room light can be avoided.

• Install the microscope in a location with a room temperature of 0°to 40°C and with a

maximum relative humidity of 85%. High temperature and humidity are to be avoided

because they promote mold growth and condensation, which may damage the microscope.

• Dirt and dust degrade optical performance and are to be avoided.

• Vibrations in the environment will degrade the image. Install the microscope in a location

free of vibrations.

• Install the microscope on a solid table and keep the microscope level.

• Select a layout that allows easy detachment of the power cord from the AC inlet of this

microscope in the event of emergency.

• This microscope emits a weak electromagnetic wave. Do not place a precision electronic

device near the microscope as precision could be degraded. Also, avoid placing a radio or TV

near the microscope as reception of sound and images may be hampered.

（2）Carrying the Microscope

This microscope is a precision instrument. Handle it

gently. Strong shocks and forcible operation will

damage the instrument. Shocks to the objectives,

especially, could degrade image precision.

• When carrying the microscope, hold it at its upper

rear and lower front ends.

• Do not hold the focus knobs, the eyepiece tube, or

the stage. These parts could easily come off and

could result in malfunctions.

（3）Handling the Lamp

Do not touch the glass part of the lamp with bare hands. Wear gloves or use a cloth when

handling the lamp so as not to leave fingerprints on the surface. Wipe off any fingerprints or

stains using a clean cloth moistened with alcohol. Fingerprints will etch into the hot surface of

the lamp and reduce the brightness, damage the lamp or reduce its service life.

Handle the lamp gently. Shocks and vibrations will damage the lamp or reduce its service life.

When changing the lamp, be sure that the contact is not damaged. If the contact is damaged,

the lamp may not light up or may overheat. Insert the lamp’s contact pins fully into the socket

holes. If the pins are loose, the lamp could come off or result in a contact failure, which will

cause overheating or smoke. Also, make sure that the field lens unit is securely attached.

Do not break the used lamps; instead dispose of them as special industrial waste or according to

the laws applicable to your municipal waste system.

Safety Precautions

Notes on Handling the System

（4）Refocusing

When changing specimens using the refocusing

mechanism, gently lower the stage by hand taking

care not to hit the field lens with the condenser holder

(p. 15).

（5）Focus Knobs

Do not turn the right and left focus knobs

simultaneously in opposite directions. Do not turn the

coarse focus knob any further after the stage has

been moved up or down to its limit. These operations

will damage the focusing mechanism.

(The coarse focus knob has a protection device. The

knob turns freely for a while after it has reached its

upper limit.)

（6）Oil-Immersion Observation

Use only a minimum quantity of oil. If too much oil is applied, surplus oil could flow out to the

stage and the condenser which could lead to degraded performance.

When using petroleum benzine or absolute alcohol to wipe off immersion oil or

to clean the lenses, follow the instructions provided by their manufacturers.

Absolute alcohol and petroleum benzine are inflammable. Take great care when

handling them.

（7）Shipping Clamps

The microscope is held tightly by the clamps

during shipment. Be sure to remove the

clamps before use. For details, see p. 35.

Make sure not

to hit the field

Do not turn the knobs in o

osite directions.

Shi

Clam

Shi

Clam

sShi

Clam

WARNING

Contents

Safety Precautions .............................................................................................. 2

I Nomenclature of Each Part............................................................................. 8

II Switches and Controls ..................................................................................10

III A Quick Microscopic Procedure.......................................................................12

IV Microscopy (Detailed Procedure) ....................................................................14

V Miscellaneous Operations..............................................................................31

1 Oil-Immersion Observation................................................................................... 31

2 Adjusting the Torque of the Coarse Focus Knob ....................................................... 32

3 Upper Limit Bolt .................................................................................................. 33

4 Cord Hangers...................................................................................................... 33

VI Assembly ...................................................................................................34

1 Assembly ........................................................................................................... 34

1) Input Voltage Check ...................................................................................... 34

2) Removal of Shipping Clamps .......................................................................... 35

3) Attaching the Intermediate Tube ..................................................................... 36

4) Attaching the Eyepiece Tube and the Eyepieces................................................. 36

5) Attaching the Analyzer Slider.......................................................................... 37

6) Attaching the Objectives ................................................................................ 37

7) Attaching the Condenser ................................................................................ 38

8) Connecting the Power Cord ............................................................................ 38

9) Other Accessories ......................................................................................... 38

2 Replacement of Consumable Materials ................................................................... 39

1) Replacing the Lamp ....................................................................................... 39

2) Replacing the Fuse ........................................................................................ 41

VII Optical Characteristics..................................................................................42

VIII Troubleshooting tables .................................................................................44

IX Care and Maintenance..................................................................................49

X Technical Specifications ................................................................................50

Nomenclature of Each Part

The microscope is made up of the following components.

(1) Basic unit

(2) Eyepieces

(3) Eyepiece tube

(4) Objectives

Objectives with various magnifying powers are available.

(5) Condenser

Used for condensing light. The condenser should be positioned slightly lower than its

upper limit.

Adjust the aperture diaphragm ring according to the objective.

(6) Polarizing intermediate tube

Used with the analyzer slider and the P-CL plate attached.

Equipped with the Bertrand lens.

(7) Polarizer

Adjust the vibration direction together with the analyzer before use.

(8) Field lens unit

Draw out the field lens unit when changing lamp.

(9) Lamp

Halogen lamp 6 V-20 W or 6 V-30 W is used.

(10) Fuse

Two 250 V 1 A time-lag low-breaking type fuses are used.

(11) Power cord

Use the power cord provided.

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