Waters ACQUITY UPLC Betriebsanleitung
ACQUITY UPLC
Column Compartments Operator’s Overview
and Maintenance Information
Revision C
Copyright © Waters Corporation 20102011
All rights reserved
ii
Copyright notice
© 20102011 WATERS CORPORATION. PRINTED IN THE UNITED
STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS
DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY
FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and
should not be construed as a commitment by Waters Corporation. Waters
Corporation assumes no responsibility for any errors that may appear in this
document. This document is believed to be complete and accurate at the time
of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use.
Considerations specific to the column compartments
Warning: Do not use the column heater with solvents that have auto
ignition temperatures below 150 C.
Warning: When dealing with hazardous solvents, run the system in a
hooded environment that provides a sufficient air exchange rate.
Warning: To prevent injury, always observe Good Laboratory Practices
when you handle solvents, change tubing, or operate the column heater.
Consult the Material Safety Data Sheets and the Solvent
Considerations section of the ACQUITY UPLC System Guide regarding
the solvents you use.
Table of Contents iii
Copyright notice ................................................................................................... ii
Considerations specific to the column compartments ................................. ii
Overview ................................................................................................................ 1
Column compartment types ................................................................................ 1
Preparing the column compartments ........................................................... 14
Installation recommendations for fittings........................................................ 14
Installing the standard column heater............................................................. 17
Installing the 30-cm column heater.................................................................. 39
Installing the column manager......................................................................... 45
Installing one CM-Aux module, in a single-stack configuration..................... 48
Installing two CM-Aux modules in a multiple-stack configuration................ 54
Installing the ACQUITY 30-cm column heater/cooler..................................... 82
Installing the optional column in-line filter ..................................................... 87
Installing the column......................................................................................... 89
Using the column compartments ................................................................... 98
Control panels .................................................................................................... 98
Resolving column compartment leak sensor errors ......................................... 99
Maintaining the column compartments ..................................................... 110
Contacting Waters technical service............................................................... 110
Maintenance schedule ..................................................................................... 112
Maintenance considerations............................................................................ 113
Configuring maintenance warnings ............................................................... 113
Replacing the column heater’s leak sensor .................................................... 114
Replacing the 30-cm column heater’s leak sensor ......................................... 119
Replacing the column manager’s leak sensor................................................. 119
Replacing the column manager switching-valve cartridge............................ 122
Replacing the column ...................................................................................... 125
Replacing the active preheater assembly ....................................................... 127
Replacing the column stabilizer assembly (CH-A only)................................. 133
Replacing the frit in the in-line filter unit...................................................... 136
Table of Contents
Overview 1
Overview
Column temperature variations can shift peak retention times and alter peak
shapes, increasing the difficulty of achieving precise results. The ACQUITY
UPLC®column compartments help to ensure precise, reproducible
separations by controlling the column temperature.
Note: If you are using an ACQUITY UPLC system with two dimensional
applications, refer to the ACQUITY UPLC Systems with 2D Technology
Capabilities Guide.
Column compartment types
The following column compartments are available for use with ACQUITY
UPLC systems:
• Column heater (CH-A)
• 30-cm column heater (CH-30A)
• Column manager (CM-A)
• Auxiliary column manager (CM-Aux)
• 30-cm column heater/cooler (ACQUITY UPLC 30-cm CHC)
Each of these column compartments is also available in a bio-compatible
version.
Column heater (CH-A)
A standard component of the basic ACQUITY UPLC system, the column
heater heats the column compartment to any temperature, from 20ºC to
90 ºC, that is at least 5 ºC above the ambient temperature. A standard, active
preheater heats the incoming solvent before it enters the column.
Additionally, you can install a supplementary column stabilizer.
The column heater can accommodate UPLC®columns with dimensions of as
much as 4.6 mm, internal diameter (ID), and 150-mm long.
To reduce dispersion associated with dead volume, and minimize the length of
tubing between system instruments, the column compartment is directly
above the sample manager and connected to the optical detector (on top of the
column heater). For MS applications, you can mount the column
compartment, on a bracket to the right-hand side of the system stack,
reducing the distance to the mass spectrometer’s inlet probe.
2
A receptacle on the column heater’s right-hand side receives the column's
eCord™ chip. The eCord column chip stores column manufacturing and usage
data, which you can access from the ACQUITY UPLC Console.
The column heater does not have its own power supply; it draws power from
the sample manager. Thus the column heater is automatically powered-on
when you power on the sample manager.
Restriction: The ACQUITY column heater (CH-A) cannot be used in
conjunction with the optional 30-cm column heater (CH-30A).
Active preheater
The standard, active preheater uses an electric heating element to directly
heat the column inlet tubing. The rapid heating, low volume design reduces
gradient delay and extra-column bandspreading. It ensures that high flow
rate separations occur at the column temperature, not at the temperature of
the incoming solvent. By controlling the temperature of the mobile phase at
the point of use—the column inlet—temperature differences introduced by
variations in the system configuration are eliminated.
Active preheater:
If you use the active preheater when running an HPLC or UPLC method
originally developed for use with a column stabilizer, adjusting the column
temperature can be necessary.
Overview 3
The following diagram shows the location of the column heater in the
ACQUITY UPLC system.
TP03240
Bottle tray
Detector
Column heater (CH-A)
Sample manager - flow through needle /
Sample manager - fixed loop
Solvent manager
4
Column heater, major components:
The following diagram shows the column heater’s major components.
Column heater components:
Component Description
Column
compartment
Contains the column, column heater, in-line filter, and
active preheater or column stabilizer assembly. The
column compartment insulates the column from changes
in ambient temperature. For MS applications, the
assembly can be mounted on an external bracket on the
right-hand side of the chassis, reducing the distance to
the inlet probe of a mass spectrometer.
In-line filter Protects the analytical column against contamination by
trapping impurities before they reach the column.
Active preheater
assembly
Heats the incoming solvent to the specified column
temperature before the solvent enters the column.
Active preheater
key
In the 30-cm column heater, installed in the control port
not occupied by the active preheater.
Active preheater
retainer
Secures the active preheater in the column
compartment.
eCord receptacle Receives the column eCord™ chip, which stores column
manufacturing and usage data.
Column
compartment tray
Location of
column
Fitting Active preheater
retainer
Active preheater
assembly
Overview 5
30-cm Column heater (CH-30A)
The optional 30-cm column heater heats the column compartment to any
temperature, from 20ºC to 90 ºC, that is at least 5 ºC above ambient. The
30-cm column heater mounts on the side of the system stack.
An active preheater heats the incoming solvent before it enters the column.
The active preheater can be located at the top or bottom of the column
compartment, to accommodate UPLC or HPLC columns. You can disable the
active preheater when solvent pre-heating is unnecessary.
Column stabilizer
(optional)
Passively heats the incoming solvent before the solvent
enters the column.
Drip tray Captures any leakage from the column or column
connections and routes it into the drip management
system.
Column heater components: (Continued)
Component Description
6
The column heater can accommodate UPLC®columns of 4.6 mm, or less, ID
and 150 mm, or less, length and HPLC columns of 4.6 mm, or less, ID and
300 mm, or less, length. For columns that exceed 4.6 mm ID, use the
ACQUITY UPLC 30-cm column heater/cooler with the appropriate
compatibility kit.
A receptacle atop the column heater receives the column’s eCord™ chip. The
chip stores column manufacturing and usage data, which you can access from
the ACQUITY UPLC Console.
The column heater does not have its own power supply; it draws power from
the sample manager. Thus the column heater is automatically powered-on
when you power on the sample manager. Do not use the 30-cm column heater
(CH-30A) in conjunction with the standard ACQUITY column heater (CH-A).
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