WPI REG2 Bedienungsanleitung

INSTRUCTION MANUAL

Serial No._____________________

030813

Regal Pipettes

World Precision Instruments

www.wpiinc.com

Regal Pipetters

World Precision Instruments iii

may be reproduced or translated into any language, in any form, without prior written permission of

World Precision Instruments, Inc.

CONTENTS

ABOUT THIS MANUAL ..................................................................................................................... 1

INTRODUCTION.................................................................................................................................. 2

Cautions and Warnings .............................................................................................................. 2

Parts List ........................................................................................................................................... 2

Single Channel Pipettes......................................................................................................... 2

INSTRUMENT DESCRIPTION.......................................................................................................... 3

Single/Multi-Channel Crystal Pipettes.................................................................................... 3

Setting the Dispense Volume....................................................................................................4

OPERATING INSTRUCTIONS........................................................................................................... 4

Aspirating Liquid .......................................................................................................................... 4

Dispensing Liquid.......................................................................................................................... 5

Exchanging Tip Ejector Unit (Single channel only)............................................................ 5

MAINTENANCE .................................................................................................................................. 6

Calibrating the Pipette................................................................................................................. 6

ACCESSORIES....................................................................................................................................... 7

Pipette Tips (Universal)............................................................................................................... 7

SPECIFICATIONS.................................................................................................................................. 9

Single Channel Pipettes .............................................................................................................. 9

WARRANTY ........................................................................................................................................11

Claims and Returns ....................................................................................................................11

Repairs ............................................................................................................................................11

iv World Precision Instruments

Regal Pipetters

World Precision Instruments 1

ABOUT THIS MANUAL

The following symbols are used in this guide:

This symbol indicates a CAUTION. Cautions warn against actions that can

cause damage to equipment. Please read these carefully.

This symbol indicates a WARNING. Warnings alert you to actions that can

cause personal injury or pose a physical threat. Please read these carefully.

NOTES and TIPS contain helpful information.

Fig. 1—The Regal Pipetters come in a variety of sizes.

2 World Precision Instruments

INTRODUCTION

Regal Pipettes use the air displacement method (where displaced air volume by

the motion of the piston is equal to the liquid volume drawn into the pipette). They

are highly accurate. Within ten complete revolutions of the dial, you can set the

minimum and maximum volumes. For ease of use, the dial is ﬁxed to the plunger.

The light plunger action and ergonomic handle reduce fatigue, making results more

precise. Regal pipettes are available as in a variety of sizes up to 10mL.

Cautions and Warnings

CAUTION: Do not autoclave the pipettes.

CAUTION: Do not use pipettes with organic solvents.

CAUTION: Do not lay the pipette down when a ﬁlled tip is attached.

CAUTION: NEVER use a pipette without a tip attached.

Parts List

After unpacking, verify that there is no visible damage to the product. Verify that all

items are included.

Single Channel Pipettes

(1) Regal Pipette

(1) Calibration tool

(1) package of disposable pipette tips

(1) Instruction Manual

(1) Inspection Certiﬁcate

Unpacking

Upon receipt of this instrument, make a thorough inspection of the contents and

check for possible damage. Missing cartons or obvious damage to cartons should be

noted on the delivery receipt before signing. Concealed damage should be reported

at once to the carrier and an inspection requested. Please read the section entitled

“Claims and Returns” on page 11 of this manual. Please contact WPI Customer

Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com.

Regal Pipetters

World Precision Instruments 3

Returns: Do not return any goods to WPI without obtaining prior approval (RMA

# required) and instructions from WPI’s Returns Department. Goods returned

(unauthorized) by collect freight may be refused. If a return shipment is necessary,

use the original container, if possible. If the original container is not available, use a

suitable substitute that is rigid and of adequate size. Wrap the instrument in paper

or plastic surrounded with at least 100mm (four inches) of shock absorbing material.

For further details, please read the section entitled “Claims and Returns” on page

11 of this manual.

INSTRUMENT DESCRIPTION

Single/Multi-Channel Crystal Pipettes

Nose Coneo

Tip Ejector Unit

r Un

ect

Connecting Nut

Tip Ejector Button

Push Button

Adjusting Dial

First Stop Position

Button

osition

Second Stop Position

Btt

cto

PhB

Push B

Ejec

Tip

Sto

First

Seco

Fig. 2—Single channel Regal pipettes

4 World Precision Instruments

Push Button–The push button is designed with two stop positions. The ﬁrst stop

position displaces the air volume indicated on the dial. The second stop position

dispenses the liquid retained in the tip.

Adjustment Dial–Turn the dial to the right (clockwise) to increase the dispensing

volume.

Tip Ejector Button–Push the button down to eject the pipette tip.

Connecting Nut–Secures the tip ejector unit. DO NOT REMOVE THIS NUT.

Tip Ejector Unit–This section houses the inner workings of the pipette. DO NOT

DISASSEMBLE.

Nose cone–Position a disposable pipette tip on the nose cone.

Setting the Dispense Volume

Rotate the adjustment dial or the push button to dial in the aliquot volume you want

to dispense. Rotate the dial to the left to decrease and to the right to increase the

volume. An example of each pipette display below shows how to read the volume

set for each pipette.

REG2 REG10 REG20 REG50 REG100 REG200 REG1000 REG5K REG10K

101001000

272572727

555055555

1.25L 7.5L 12.5L50L75L 125L 750 2500L 7.5mL

OPERATING INSTRUCTIONS

Aspirating Liquid

1. Adjust the delivering volume by turning the adjusting dial to the desired volume.

2. Install the appropriate pipette tip on the nose cone (Fig. 3). The table below

shows which tips are compatible with Regal pipettes.

Correct

Position

Fig. 3—Proper position for setting the tips

Regal Pipetters

World Precision Instruments 5

Regal Tip Compatibility Chart

Pipette WPI Universal Tips Stands

REG2, REG10 500191, 500192, 500199, 500202, 500203,

500204

504591

REG20 500191, 500192, 500193, 500194, 500199,

500200, 500202, 500203, 500204, 500205,

500206

REG50, REG100,

REG200

500193, 500194, 500200, 500205, 500206,

500207

REG1000 500195, 500196, 500201

REG5K 500197, 500198

REG10K 504588, 504589, 504590

3. Press the push button down to the ﬁrst stop position.

4. Hold the pipette vertically and immerse the tip into the solution so that it is just

deep enough to draw in the liquid (about 2–4mm).

5. After 2–3 seconds, slowly release the push button and allow it to return to the

initial position. The solution is drawn up after a brief interval.

6. Slowly remove the tip from the liquid, keeping the pipette in a vertical position.

CAUTION: Do not lay the pipette down when a ﬁlled tip is attached.

Dispensing Liquid

1. To dispense the liquid, position the pipette tip close to the inner wall of the

receiving vessel and press the push button down to the ﬁrst stop position.

2. Press the push button down to the second stop position to remove all the liquid

from the tip.

3. Press the tip ejector button to remove the used pipette tip.

Exchanging Tip Ejector Unit (Single channel only)

NOTE: The CRYSTAL-ML10 has no tip ejector.

• To remove the tip ejector unit, hold the tip ejector button down and pull out

the tip ejector unit from the ejection rod along the nose cone. Do not lose the

spring on the rod.

• To install the tip ejector unit, hold down the tip ejector button and check the

position of ejector connection. While holding down the tip ejector button, ﬁt the

tip ejector unit on the rod. Push the ejector unit ﬁrmly onto the rod.

CAUTION: The stopper on the tip ejector button may be damaged if you do

not hold down the tip ejector button while reassembling.

6 World Precision Instruments

MAINTENANCE

There is no need to dis-assemble the pipette. Disassembly voids the warranty.

Calibrating the Pipette

Pipettes should be calibrated annually. The accuracy of the calibration depends

on the equipment used and your attention to detail. For best results, follow these

guidelines:

• Use distilled water for calibration.

• Make sure the pipette, tips, water and balance are all at the same room

temperature. Keep them in the same location for two hours prior to calibration.

• Atmospheric pressure and room temperature can affect the calibration

measurements. Many calibration software packages are available. Using such

software allows you to correct for the environmental effects.

1. Set the pipette to the maximum volume. Place a disposable tip on the pipette.

Using a balance, measure the weight of three successive aliquots of distilled

water to the nearest 0.0001 of a gram.

2. Record each value and then average the three values.

3. Set the pipette to its minimum volume, and repeat steps 1–2.

4. Locate the calibration tool that came with your pipette. Clip it onto the pipette

shaft with the teeth pointed toward the pipette body. Then, line up the teeth

with the holes in the top of the adjustment dial.

Fig. 4—(Left) The calibration tool came with your pipette.

Fig. 5—(Center) Clip the calibration tool on the shaft of the pipette push button.

Fig. 6—(Right) Push the teeth on the calibration tool into the holes in the adjustment dial.

5. Rotate the calibration tool counter-clockwise to increase the aliquot dispensed

and clockwise to decrease it.

6. Repeat steps 1–3 until the minimum and maximum volumes are accurate.

Regal Pipetters

World Precision Instruments 7

ACCESSORIES

REG2 Regal Pipette, 0.2–2L

REG10 Regal Pipette, 1–10L

REG20 Regal Pipette, 2–20L

REG50 Regal Pipette, 5–50L

REG100 Regal Pipette, 10–100L

REG200 Regal Pipette, 20–200L

REG1000 Regal Pipette, 100–1000L

REG5K Regal Pipette, 1000–5000L

REG10K Regal Pipette, 1–10mL

503787 Silicone grease

GERS5 Regal Pipetters (set of any 5) & stand

GERS6 Regal Pipetters (set of any 6) & stand

GERS7 Regal Pipetters (set of any 7) & stand

504591 Stand for Regal Pipetters (holds 8)

Pipette Tips (Universal)

Standard Universal Tips

Tip Volume For Pipetter Bulk Part No. Rack Part No.

0.1 - 10 μL

REG2

REG10

REG20

Bag of 1000 500191 960

(10 racks of 96) 500192

5 - 200 μL

REG20

REG50

REG100

REG200

Bag of 1000 500193 960

(10 racks of 96) 500194

100-1000 μL REG1K Bag of 1000 500195

1000

(10 racks of 100)

500196

500 - 5000 μL

REG5K Bag of 250 500197 500

(10 racks of 50) 500198

1000 - 10,000 μL

REG10K Bag of 1000 504588 250

(10 racks of 25) 504589

Universal Filter Tips (Sterile)

Tip Volume For Pipetter Rack Part No.

0.1 - 10 μL

REG2

REG10

REG20

960 (10 racks of 96) 500199

10 - 200 μL

REG20

REG50

REG100

REG200

960 (10 racks of 96) 500200

100-1000 μL REG1K

1000 (10 racks of 100)

500201

1000 - 10,000 μL

REG10K 250 (10 racks of 25) 504590

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