Agilent Technologies AssayMAP 96AM Betriebsanleitung



AssayMAP 96AM

Wash Station

Maintenance Guide

Notices

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Installation Guide Part Number

G5542-90004

Edition

Revision B, February 2018

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AssayMAP 96AM Wash Station

Maintenance Guide



96AM Wash Station Maintenance

Guide

This guide contains the following topics:

•“About this guide” on page 2

•“About the 96AM Wash Station” on page 4

•“Required resources and workflow” on page 4

•“Determining if the chimneys are in good condition” on page 6

•“Emptying the 96AM Wash Station” on page 6

•“Uninstalling the 96AM Wash Station” on page 10

•“Replacing damaged chimneys” on page 11

•“Installing the wash station and connecting the tubing” on

page 13

•“Testing the 96AM Wash Station” on page 16

1 96AM Wash Station Maintenance Guide

About this guide

2AssayMAP 96AM Wash Station Maintenance Guide

About this guide

Who should read this guide

The instructions in this guide assume that you have the following:

•An understanding of the AssayMAP Bravo Platform

•Familiarity with the Protein Sample Prep Workbench

•Experience in working on mechanical equipment

What this guide covers

This guide describes how to inspect and replace chimneys in the 96AM Wash

Station.

Figure 96AM Wash Station

The 96AM Wash Station is a component of the AssayMAP autofilling station, as

the following figure shows.

Figure AssayMAP autofilling station, including 96AM Wash Station, Pump Module, tubing, and carboys

Source Waste

1 96AM Wash Station Maintenance Guide

About this guide

AssayMAP 96AM Wash Station Maintenance Guide

Related user guides and where to find them

Accessing the user documentation

You can access the user documentation in the Protein Sample Prep Workbench

as follows:

•Application user guides. In the Protein Sample Prep Workbench, click Utility

Library, locate the utility of interest, and then click Instructions.

•Utility instructions. In the Protein Sample Prep Workbench, click Literature

Library, locate the topic of interest, and then click Open.

Alternatively, you can access the user documentation in the VWorks Knowledge

Base using either of the following methods:

•From within VWorks software, select Help > Knowledge Base or press F1.

•From the Windows desktop, do one of the following:

–Windows 10. Select Start > All Apps > Agilent Technologies > VWorks

Knowledge Base.

–Windows 7. Select Start > All Programs > Agilent Technologies > VVWorks >

User Guides > VWorks Knowledge Base.

•Go to www.agilent.com/chem/askb.

For information on… See…

Potential safety hazards and how to

avoid them

Bravo Platform Safety and

Installation Guide

Using the Protein Sample Prep

Workbench

Literature Library in the Protein

Sample Prep Workbench

Setting up, operating, maintaining, and

troubleshooting the Bravo Platform

Bravo Platform User Guide

Setting teachpoints for the AssayMAP

Bravo Platform

Bravo Platform User Guide

Setting up the Pump Module and the

autofilling reservoirs and wash stations

Pump Module User Guide

Creating and editing VWorks protocols

and application interfaces

VWorks Automation Control User

Guide

Running a syringe test of the Bravo

96AM Head

AssayMAP Syringe Test Utility

User Guide in the Protein Sample

Prep Workbench

Replacing a defective syringe in the

Bravo 96AM Head

AssayMAP Bravo Syringe

Replacement User Guide

1 96AM Wash Station Maintenance Guide

About the 96AM Wash Station

4AssayMAP 96AM Wash Station Maintenance Guide

About the 96AM Wash Station

The 96AM Wash Station has 96 chimneys and is scaled to work with the

syringe probes in the Bravo 96AM Head. The wash station is used for washing

the syringes and washing cartridges or pipette tips that are seated on the

syringe probes.

Figure Chimney detail in the 96AM Wash Station

Fresh wash liquid enters the 96AM Wash Station through two inlet ports (1),

shown in the following figure. The wash liquid flows up through the chimneys,

and the waste overflows from the chimneys and is removed through two outlet

ports (2).

Figure Inlet ports (1) and outlet ports (2) in the 96AM Wash Station

Required resources and workflow

Ensure that you have the following tools and materials:

•Replacement Wash Station Chimneys: large internal diameter, pack 100,

Agilent part number 15586- 002

•Hex driver to uninstall and install 2- mm hex screws

•1 needle- nose pliers to remove the bad chimneys from the 96AM Wash

Station

•Small rubber pad or foam to protect your thumb when seating a new

chimney in the 96AM Wash Station.

CAUTION Ensure that you use only the 96AM Wash Station on the AssayMAP Bravo

Platform. Using a model of the Tip Wash Station other than the 96AM Wash Station can result

in a potential crash with the Bravo 96AM Head.

WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they

brush across your hand. A probe scratch can expose you to any contaminants remaining on

the probes. Wear gloves and use extreme caution to avoid brushing against the probes.

1 96AM Wash Station Maintenance Guide

Required resources and workflow

AssayMAP 96AM Wash Station Maintenance Guide

Perform the following steps in the order given.

Step For this task... See...

1Inspect the chimneys for damage and

irregularities.

“Determining if the

chimneys are in good

condition” on page 6

2Empty the wash station. “Emptying the 96AM Wash

Station” on page 6

3Uninstall the wash station. “Uninstalling the 96AM

Wash Station” on page 10

4Replace the damaged chimneys. “Replacing damaged

chimneys” on page 11

5Install the wash station. “Installing the wash station

and connecting the tubing”

on page 13

6Verify the wash station teachpoint

and adjust it, as necessary:

aInitialize the AssayMAP 1

profile and adjust the

teachpoint for the 96AM Wash

Station.

bUse the AssayMAP Teachpoint

Update protocol to update the

teachpoint in the remaining

profiles in the Protein Sample

Prep Workbench

automatically.

AssayMAP Bravo Platform

Installation Guide (for field

service personnel) or

Bravo Platform User Guide

7Start up the AssayMAP Bravo

Platform and verify that all the

chimneys work consistently.

“Testing the 96AM Wash

Station” on page 16

1 96AM Wash Station Maintenance Guide

Determining if the chimneys are in good condition

6AssayMAP 96AM Wash Station Maintenance Guide

Determining if the chimneys are in good condition

During normal operation of the 96AM Wash Station, the fluid flow out of the

top of each chimney and the shape of the fluid dome at the top of each

chimney must consistent. To ensure consistency in the fluid flow from each

chimney, the chimneys must be:

•Clean and free of any debris.

•Free of deformities or irregularities.

See the good condition chimneys in the following figure. The chimneys

must not be damaged, such as those shown in the figure (possibly from a

head collision).

•Equal in height and installed in the same orientation.

Figure Close up views of 96AM Wash Station chimneys

Chimney irregularities or damaged chimneys can cause large droplets to cling

to the syringe probes, which can introduce variability in the assay.

Emptying the 96AM Wash Station

This topic describes how to use the Pump Reagent task in Bravo Diagnostics to

empty any fluid from the wash station.

Before you start

WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they

brush across your hand. A probe scratch can expose you to any contaminants remaining on

the probes. Wear gloves and use extreme caution to avoid brushing against the probes.

WARNING When you initialize the Bravo Platform, the head and tie bar can move. To

prevent potential injury, keep clear of the device while it is in motion.

CAUTION To prevent potential equipment damage, ensure that the deck is clear of any

obstacles before initializing the AssayMAP Bravo Platform.

1 96AM Wash Station Maintenance Guide

Emptying the 96AM Wash Station

AssayMAP 96AM Wash Station Maintenance Guide

Running the Pump Reagent task to empty the wash station

To empty the wash station using the Pump Reagent task:

1At the carboy that contains the wash solution, remove the lid and lift it so

that the tubing is above the liquid level.

2Ensure that the Protein Sample Prep Workbench is closed, and then start

the VWorks software.

You can use the VWorks desktop icon or the Start menu on the Windows

desktop to start the VWorks software.

3Ensure that Simulation is off.

4In the VWorks window, open the AssayMAP Bravo device file

(AssayMAP Bravo.dev).

To do this, click File > Open. In the Open dialog box, ensure the file type is

Device Files (*.dev), select the file, and then click Open.

The device files are installed in the following default location:

C:\VWorks Workspace\Workbench\Device Files

5Click No when the Would you like to initialize devices now? message appears.

6In the AssayMAP Bravo.dev tab, click Device diagnostics. The Bravo Diagnostics

dialog opens.

CAUTION Using an incorrect profile can cause equipment damage. Ensure that the profile

is correct for the head type and deck configuration.

1 96AM Wash Station Maintenance Guide

Emptying the 96AM Wash Station

8AssayMAP 96AM Wash Station Maintenance Guide

Figure AssayMAP Bravo.dev tab and Profile setting for AssayMAP 1

7Click the Profiles tab, select the Profile name AssayMAP 1, and then click

Initialize this profile.

8If the There appears to be a plate present error message appears, click Ignore and

Continue to continue the homing process.

9When the Verify that it is safe to home the W-axis message appears, click Retry to

continue homing the w-axis.

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