Agilent Technologies 1100 Series Bedienungsanleitung

Agilent 1100 Series

Nano Pump

User Manual

ii 1100 Series Nano Pump User Manual

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G2226-90000

Edition

Edition 07/02

Printed in Germany

Agilent Technologies, Deutschland GmbH

Hewlett-Packard Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Techn ology Li cense s

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

Safety Notices

CAUTION

A CAUTION notice denotes a hazard.

It calls attention to an operating pro-

cedure, practice, or the like that, if

not correctly performed or adhered

to, could result in damage to the

product or loss of important data. Do

not proceed beyond a CAUTION

notice until the indicated conditions

are fully understood and met.

WARNING

A WARNING notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in personal

injury or death. Do not proceed

beyond a WARNING notice until the

indicated conditions are fully

understood and met.

Warning Symbols Used on the

Instrument

The apparatus is marked with this

symbol when the user should refer to

the instruction manual in order to

prevent risk of harm to the operator

and to protect the apparatus against

damage.

1100 Series Nano Pump User Manual iii

In This Manual…

This manual contains information for using your Nano Pump.

1 Introduction - Around your nano pump

In this chapter you will find an introduction to the nano pump

and some hardware details to identify the parts of the pump

and their function.

2 Getting started

In this chapter you will find road maps to help you to start an

analysis with the nano pump. In addition it provides different

ChemStation screens necessary to configure and start an

analysis with the nano pump.

Follow the step by step priming procedure before using the

nano pump in micro mode.

3 Become an expert

In this chapter you will find hints to optimize your nano LC

system to achieve best chromatographic results. It also offers

diagnosis and build-in tests for maintenance features.

Annex A Safety Information

iv 1100 Series Nano Pump User Manual

1100 Series Nano Pump User Manual v

Contents

1 Introduction - Around your Nano Pump

Introduction to the nano pump 2

Nano Pump Main Overview 3

Pumphead Overview 4

Flow connections 5

Fittings and ferrules 6

Electrical connections 7

2 Getting started

Road maps to start an analysis with the nano pump 10

Road map to configure the nano pump 10

Road map to prepare the nano pump for an analysis 10

Road map to start an analysis 11

Using the ChemStation to Configure your Pump 12

Pump configuration screen 12

Pump auxiliary screen 13

Setup pump parameter screen 15

Priming the pump for best results 20

Manually Priming the Solvent Channels. 20

Purging the Pump 21

3 Become an expert

Hints for Successful Use of the Capillaries 26

Hints for Successful Use of the Micro Vacuum Degasser 28

vi 1100 Series Nano Pump User Manual

Hints for Successful Use of the Nano Pump 29

Hints for Using the Fast Composition Change/Reconditioning 30

Purpose 30

How the Function Works 30

Hints for Successful Use of the Micro Well Plate Sampler 32

Hints for Successful Use of the Column and Switching Valve

Compartment 33

Best Practices for Filters 34

The solvent inlet filters 34

Inline filter 35

Hints for Successful use of Solvents and Mobile Phase 36

Hints for Choosing the Primary Flow 37

Hints to Optimize the Compressibility Compensation Setting 39

Diagnosis screens 41

Test screens 44

EMF Screen 45

ASafetyInformation

Safety Information 48

General 48

Operation 48

Safety Symbols 49

Lithium Batteries Information 51

Danish Information 51

Radio Interference 52

Test and Measurement 52

Sound Emission 53

Manufacturer’s Declaration 53

1100 Series Nano Pump User Manual vii

Solvent Information 54

Solvents 54

Agilent Technologies on Internet 55

Index 57

viii 1100 Series Nano Pump User Manual

Agilent 1100 Series Nano Pump

User Manual

Agilent Technologies

Introduction - Around your Nano Pump

Introduction to the nano pump 2

Nano Pump Main Overview 3

Pumphead Overview 4

Flow connections 5

Fittings and ferrules 6

Electrical connections 7

In this chapter you will find an introduction to the nano pump and hardware

details to identify the parts of the pump and their function.

For more information on how to install a nano pump in direct injection mode,

refer to the Service Manual G2226-60100 Chapter 1, Installing the Pump.

For more information on the different stacking possibilities, for a nano pump

included in an online sample enrichment LC/Ion trap MS, refer to the

Orientation Guide G2228-90000.

2 1100 Series Nano Pump User Manual

1 Introduction - Around your Nano Pump

Introduction to the nano pump

The nano pump consists of two identical pumping units in a single housing. It

generates gradients by high-pressure mixing. A solvent selection valve

provides flexibility in the choice of solvents.

Mobile phase composition is produced by mixing the outputs of pump A and

pump B. The solvent selection valve allows the pump A output to originate

from either channel A1 or channel A2. The pump B output may originate from

either channel B1 or channel B2.

The primary flow produced by the two pumping units is proportioned in an

electromagnetic proportional valve (EMPV). The remaining column flow is

measured in a mass flow sensitive flow sensor. The measured flow is

compared with the user-entered column flow setpoint. The flow sensor

controls the EMPV current, causing the EMPV to correctly proportion the

column flow. The primary flow in excess of the required column flow volume

is directed to the waste.

Solvent degassing is not done directly in the pump. A 4-channel, low volume

micro vacuum degasser, available as a separate module, provides degassed

solvents to the pump channel inputs. Solvent degassing is required for best

flow stability and detector stability, especially at the low flow rates required to

run nano LC applications.

The flow range of the nano pump is between 0.1 µl/min and 1 µl/min.

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