Gilson 333 Bedienungsanleitung

User's Guide

333/334 HPLC Pumps

Trademarks

All product and company names are trademarks™ or registered® trademarks of

their respective holders. Use of the trademark(s) in this document does not imply

any affiliation with or endorsements by the trademark holder(s).

333/334 HPLC PUMPS | USER’S GUIDE

TABLE OF CONTENTS

SAFETY | 7

Electronic and Hazard Symbols | 8

Safety Notices | 8

Lifting | 9

Voltage | 9

Solvents | 9

Replacement Parts | 9

CHAPTER 1 | INTRODUCTION | 11

Description | 12

Unpacking | 15

Technical Speciﬁcations | 16

Customer Service | 18

CHAPTER 2 | INSTALLATION | 19

Electrical Connections | 20

Cabling for TRILUTION LC Control | 24

Cabling for Control from 333 Pump | 24

Twin Pump Systems | 25

Positioning the Pumps | 26

Hydraulic Connections | 28

CHAPTER 3 | OPERATION | 33

Front Panel and Startup | 34

Priming | 34

Pump Operating Parameters | 37

TABLEOFCONTENTS |333/334 HPLC PUMPS

TABLE OF CONTENTS

CHAPTER 4 | MAINTENANCE | 39

Maintenance Overview | 40

Cleaning | 40

Pump Head | 41

Piston Seals | 45

Piston and Bellows | 46

Piston Rinsing Chamber | 47

Reassemble Pump Head | 48

Check Valves | 49

Filters | 51

Maintenance Procedures | 52

Resetting Maintenance Logs | 52

Fuse Replacement | 52

CHAPTER 5 | TROUBLESHOOTING | 53

Error LED (333 Pump) | 54

Error Messages (333 Pump) | 55

Troubleshooting | 56

Repair and Return Policies | 58

APPENDIX A | REPLACEMENT PARTS | 59

333/334 HPLC Pumps | 59

Hydraulic | 59

Pump Head | 61

Electrical | 61

Miscellaneous (Tools, Power Cords, Cables, etc.) | 62

Service Part | 62

APPENDIX B | COLUMN HOLDER | 63

Installation | 63

Part Numbers | 64

333/334 HPLC PUMPS | USER’S GUIDE

TABLE OF CONTENTS

APPENDIX C | INJECTOR | 65

Injection Valve Holder | 66

Connection to Injector | 66

Part Numbers | 66

APPENDIX D | FRONT PANEL CONTROL | 67

Using the Control Panel | 68

Electrical Connections | 69

Basic Control Conﬁgurations | 70

Hardware Conﬁguration | 72

Safety Functions | 74

Electrical Contacts | 76

Manual Operations | 78

Entering Method Programs | 80

Method Program Worksheet | 84

File Management | 86

Good Laboratory Practice (GLP Functions) | 88

Running a Method Program | 92

APPENDIX E | REFERENCE INFORMATION | 93

Liquid Contact Materials | 93

Solvent Miscibility | 95

Chapter1|SaFetY

The instrument is intended to be used in a laboratory by trained technical personnel. For safe

and correct use, both operating and service personnel should follow the instructions contained

in this guide when installing, cleaning, and maintaining the instrument.

The following safety precautions must be observed during all phases of operation, service, and

repair of the instrument. Failure to comply with these precautions or with specific warnings

elsewhere in the user’s guide violates safety standards of design, manufacture, and intended

use of the instrument. Gilson assumes no liability for the customer’s failure to comply with

these requirements.

The instrument has been certified to safety standards required in Canada, Europe, and the

United States. Refer to the rear panel label on the instrument or the Declaration of Conformity

document for the current standards to which the instrument has been found compliant.

IN thIS Chapter

●Electronic and Hazard Symbols | 8

●Safety Notices | 8

●Lifting | 9

●Voltage | 9

●Solvents | 9

●Replacement Parts | 9

333/334 HPLC PUMPS | USER’S GUIDE

8SAFETY |333/334 HPLC PUMPS

SAFETY

Electronic and Hazard Symbols

The following electronic and hazard symbols may appear on the instrument:

Symbol Explanation

~Alternating current

Direct current

Protective conductor terminal

|Electrical power ON

OElectrical power OFF

Caution, risk of electric shock

Caution

Fuse

Safety Notices

The following safety notices may appear in this document:

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, may result in

serious injury

CAUTION

CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor

or moderate injury

NOTICE

NOTICE indicates a potentially hazardous situation which, if not avoided, may result in

equipment damage

333/334 HPLC PUMPS | USER’S GUIDE

SAFETY

Lifting

The instrument exceeds the weight one person can lift safely. Two or more people are required

to lift the instrument safely. Refer to the Technical Specifications for the weight. Always lift the

instrument from the base and follow any unpacking instructions provided with the instrument.

Voltage

Access to the rear panel is necessary. The instrument must be detached from all voltage

sources before service, repair, or exchange of parts. For normal operation, the instrument is to

be grounded through the AC line cord and power supply provided. Failure to do so can result

in a potential shock hazard that could result in serious personal injury.

Use only fuses with the rated current and of the specified type as listed on the rear panel label

on the instrument. The instrument must only be operated with the voltage specified on the rear

panel label of the instrument and with the grounded AC line cord and power supply provided.

Solvents

Observe safe laboratory practices when handling solvents. If working with hazardous solvents

or flammable liquids, ensure that there is proper ventilation and that adequate protection such

as safety glasses, gloves, and protective clothing are used.

If dangerous liquids are used, precautions should be taken to limit potential hazards from leaks

and/or spillage through the use of a non-flammable tray or use of a fume hood, etc.

If there is the potential of explosive gases being developed, a fume hood or other means should

be used to safely manage that risk.

Refer to the Material Safety Data Sheets for the solvents before use.

Replacement Parts

Be sure to use only replacement parts mentioned in the user’s guide. Do not repair the instrument

or change parts not listed in the user’s guide. If it is necessary to replace parts not listed, please

contact your local Gilson representative.

Dieses Handbuch passt für folgende Modelle

Inhaltsverzeichnis

Andere Gilson Laborausrüstung Handbücher

Gilson

Gilson P10M Bedienungsanleitung

Gilson

Gilson repetman Handbuch

Gilson

Gilson GX-27X Series Bedienungsanleitung

Gilson

Gilson Pipetting Aid Bedienungsanleitung

Gilson

Gilson SGA-5RT Bedienungsanleitung

Gilson

Gilson PIPETMAN G Bedienungsanleitung

Gilson

Gilson Pipetman M Bedienungsanleitung

Gilson

Gilson ASPEC GX-271 Bedienungsanleitung

Gilson

Gilson PIPETMAN P2L Bedienungsanleitung

Gilson

Gilson pipetman Bedienungsanleitung

Gilson

Gilson SS-15D Bedienungsanleitung

Gilson

Gilson P10M Bedienungsanleitung

Gilson

Gilson PIPETMAN L Bedienungsanleitung

Gilson

Gilson macroman Bedienungsanleitung

Gilson

Gilson PIPETMAN 8X200 Bedienungsanleitung

Gilson

Gilson 223 Bedienungsanleitung

Gilson

Gilson MyPIPETMAN Select Bedienungsanleitung

Gilson

Gilson Gilson Test-Master TM-5 Bedienungsanleitung

Gilson

Gilson microman e Bedienungsanleitung

Gilson

Gilson MyPipetman P2 Bedienungsanleitung

Gilson

Gilson CPC Series Bedienungsanleitung

Gilson

Gilson Trackman Bedienungsanleitung

Gilson

Gilson CENTRY 103 Minicentrifuge Bedienungsanleitung

Gilson

Gilson MICROMAN E M10E Bedienungsanleitung

Beliebte Laborausrüstung Handbücher anderer Marken

Qiagen

Qiagen DML 3000 Bedienungsanleitung

Queensgate

Queensgate NANOSCAN OP400 Programmierhandbuch

Conviron

Conviron GEN1000-GE Bedienungsanleitung

Parr Instrument

Parr Instrument 4560 Bedienungsanleitung

IKA

IKA IC control Bedienungsanleitung

Integra

Integra DOSE IT Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5800 ICP-OES Bedienungsanleitung

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Bedienungsanleitung

NI PXI-5422 Handbuch

Collomix

Collomix Aqix Bedienungsanleitung

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Bedienungsanleitung

Ocean Insight

Ocean Insight FLAME-NIR+ Bedienungsanleitung

Parker

Parker ALIGN-MG-NA Bedienungsanleitung

BD 644787 Bedienungsanleitung

DENTAURUM

DENTAURUM Compact Megaplus Bedienungsanleitung

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Bedienungsanleitung

VWR

VWR SAS Super IAQ Bedienungsanleitung

illumina

illumina MiSeqDx Bedienungsanleitung