Hach TenSette 19700-01 Bedienungsanleitung

19700-88

TenSette Pipet

Model 19700-01

©Hach Company, 1983 - 1998. All rights are reserved. 5-12-93--9ED

Rev. 1, 10/98

General Information

The Hach TenSette™ Pipet is a precision device for dispensing small quantities

of liquids. Rugged construction and precision manufacturing ensure prolonged

use and dependability with a minimum of service. Ten separate volumes (from

0.10 to 1.00 mL in 0.10-mL increments) can be selected simply by dialing the

desired setting. Reproducibility is within two percent for the 0.10, 0.20 and

0.30-mL settings. All other settings will deliver within one percent or better.

Nonwettable polyethylene pipet tips ensure quantitative transfer of aqueous

solutions.

The TenSette Pipet is factory-set to dispense sample volumes of 0.10, 0.20, 0.30,

0.40, 0.50, 0.60, 0.70, 0.80, 0.90, and 1.00 mL at an altitude of 1000 feet (305

meters) above sea level. Sample volumes will accommodate any standard

additions procedure and are well-suited for any procedure requiring precision

dispensing of small amounts of solutions.

For best results, always use a new tip for each pipetting operation. After being

used several times, the pipet tip may retain some liquid, causing an error in

delivery, Each pipet is supplied with 100 tips. Order Hach replacements, Cat.

No. 21856-96 for best results.

Always use careful and even hand movements for best reproducibility. If the

pipet does not operate smoothly, disassemble and coat the piston and retainer

with high-quality stopcock grease. The metering turret also may be lightly

coated with grease.

Sometimes the column of solution may rise in the tip while moving the pipet

between the source and the receiving vessel. This is normal and will not affect

the volume delivered as long as the proper pipetting technique is used.

For best accuracy, both the room temperature and the solution being pipetted

should be between 20 and 25ºC. Avoid palming the pipet an unnecessarily long

time prior to use because the aliquot volume could be affected by elevated

temperatures.

Never lay the pipet down with solution in the tip. Solution could leak into the

pipet and cause corrosion.

Operating Instructions

1. Attach a clean tip. Holding the TenSette in one hand, gently press the tip

onto the tapered nose of the pipet until the tip is held firmly and a good seal is

obtained.

2. Turn the turret cap to align the desired volume on the volume-setting ring

with the mark on the housing assembly.

3. Press down on the turret cap with the thumb, using a smooth motion, until the

turret reaches the stop. Immerse the tip about 5 mm (¼") below the surface of

the solution to avoid drawing air into the tip. Do not insert the tip any deeper,

or the delivery volume may be affected.

4. While maintaining a constant pressure, allow the turret to return to the

extended position. Do not let the turret snap into place, or the delivery volume

may be affected.

5. With the turret up, withdraw the tip from the liquid and move it to the

receiving vessel. Avoid placing pressure on the cap while moving the pipet.

6. Use the thumb and forefinger to twist the turret cap to the next higher position

on the volume-setting ring to assure full blowout and quantitative transfer of the

sample. The "F" position provides full blowout for the 1.0-mL setting.

Figure 1 TenSette Pipet, Exploded View

7. With the tip in contact with the side of the receiving vessel, slowly and

smoothly press down on the cap until the turret reaches the stop and the solution

is completely discharged.

Maintenance

Periodically disassemble the lower body. Clean and lubricate the piston and

retainer to ensure smooth operation. See Figure 1. During reassembly, apply a

light coat of stopcock grease to the piston shaft and o-ring seal. Slide the

coupling over the housing extension and proceed with reassembly. Tighten the

coupling hand-tight only.

If the TenSette Pipet is to be used at an altitude significantly different than 1000

feet (305 meters) above sea level, the delivery volume will be affected. See

Figure 2. The instrument can be reset for greater accuracy as follows:

1. Remove the turret cap screw, turret cap and volume-setting ring. Loosen the

turret set screw by one or two turns.

2. Place a narrow-necked vessel (for example, a 25-mL volumetric flask) on an

analytical balance and zero the balance (or record the weight if the balance

is non zeroing).

3. Weigh the quantity of water dispensed at the 0.10-mL setting. Use only

high-quality demineralized water at 20-25ºC.

4. If the weight of the water is other than 0.10 g, use a small screwdriver to

give the turret adjustment screw (inside the metering turret) a slight turn.

Turn clockwise to increase delivery volume.

5. Zero the balance (or record the weight) just prior to adding another 0.10 mL

of demineralized water. If the added weight is other than 0.10 g, repeat

Steps 3 through 5.

Figure 2 Effect of Altitude on Delivery

6. When the weight of the water delivered at the 0.10-mL setting is 0.10 g, set

the turret for a delivery of 1.00 mL.

7. Zero the balance (or record the weight) just prior to adding 1.00 mL of

water. If the weight is other than 1.00 g, adjust the delivery volume as

described in Step 4. When the pipet delivers 1.00 g at the 1.00-mL setting,

check the 0.10-mL setting again.

8. When the delivery volume is accurate for both the 1.00-mL and the 0.10-mL

settings, tighten the turret set screw and replace the volume-setting ring,

turret cap and turret cap screw.

Warranty

The Hach TenSette Pipet has been carefully engineered and constructed for

years of accurate, dependable service. It is fully guaranteed for one year.

REPAIR SERVICE

For instrument service, please contact the Hach Factory Service Center serving

your location.

In the United States: In Canada:

Hach Company Hach Sales & Service Canada Ltd.

100 Dayton Ave. 1313 Border Street, Unit 34

P.O. Box 907 Winnipeg, Manitoba

Ames, Iowa 50010 R3H 0X4

800-227-4224 (U.S.A. only) 800-665-7635

FAX: (515) 232-1276 (204) 632-5598

Telephone: (515)-232-2533 FAX: (204) 694-5134

All other locations:

Hach Company, World Headquarters

P.O. Box 389

Loveland, Colorado, 80539 U.S.A

Telephone (303) 669-3050

FAX (303) 669-2932

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