Hitachi TM4000 Technisches Datenblatt

(1) Turn on the earth leakage breaker on the back of the main unit.

(2) Turn on the power switch on the right side of the main unit to start the

device.

(3) When the unit is turned on the Evacuation process will start

automatically and the EVAC LED will blink BLUE.

If you want to load a sample, press the EVAC/AIR button. The AIR LED will

then blink WHITE. Once the specimen chamber is at air, the AIR LED will be

stop blinking and remain WHITE.

(4) Turn on the PC. Start the TM4000/TM4000Plus application by double-

clicking the TM4000 programme shortcut. The TM4000 start screen will be

displayed until the Graphical User Interface (GUI) is loaded.

Bulk specimen (Conductive/Non-conductive) Water / Oil containing specimen

(5) Put the conductive tape on the specimen stub and attach

a specimen on it. (5) When observing water containing specimens such as

biological and botanical specimens, food items and so on,

use a paste to stick a specimen on the stub.

(6) Attach the specimen stub to the specimen holder, and adjust its height using a height gauge so that the

gap width becomes about 1 mm.

TM4000/TM4000Plus

Simple Manual

Front of TM4000

1. Starting The Instrument

2. Preparing Specimen

Page 1

(7) Ensure the chamber is at air, ie AIR LED is not

blinking and is WHITE.

If the EVAC LED is Blue, press the EVAC/AIR switch. The

AIR LED (WHITE) should start blinking. When the AIR

LED stops blinking and remains illuminated the

chamber is at AIR.

3. Loading the Specimen

(8) After the AIR LED stops blinking wait approximately

five seconds or more before slowly drawing out the

specimen stage.

(9) Insert specimen stub assembly into the stage base.

(10) OPTIONAL Lightly tighten the hex screw to secure

the specimen stub assembly to the stage base.

(11) Use the X, Y knobs to centre the specimen stage

by lining up the centre position mark with the guides.

(12) Press the EVAC/AIR Switch to evacuate the

chamber. The EVAC LED will blink BLUE while the

chamber is being evacuated. Once evacuation is

complete (~2.5 min) the EVAC LED will stop blinking

and remain illuminated (BLUE)

(13) Select Accelerating Voltage / Observation condition.

4. Starting Observation

(14) Select Vacuum level

(15) Select Detector. Can change selection later so not

critical to select at this point.

(16) Click the button to turn on the high voltage.

(17) Use the auto functions

a) Set desired field of view using the X, Y knobs on the

front of the TM4000.

b) Adjust the Brightness and Contrast (click the Auto

B/C button.

c) Perform focusing (click the Auto Focus button)

a) Perform manual focus adjustment if

required.

Page 2

(18) Click the “Save” button to capture the image

5. Saving Captured Images

(20) Click the button to turn off the high voltage.

6. Stopping Observation

(21) To remove the sample press the EVAC/AIR switch to

introduce air into the specimen chamber. Wait until the AIR

LED (WHITE) stops blinking and remains illuminated.

(22) Slowly draw out the specimen stage, undo the hex

mounting screw and remove the specimen stub assembly

from the stage.

(23) Close the specimen stage door.

(24) If you are not looking at any other specimens, press the

EVAC/AIR switch to evacuate the chamber. The EVAC LED

(BLUE) will blink while the chamber is evacuated and will stop

blinking and remain illuminated once the chamber is under

vacuum (~2.5 min)

(19) After the image capturing process is completed

the Save Image dialog will be displayed. Input a file

name and save the image.

NOTE: Quick Save will save the image on the screen, ie

lower resolution than if you use the Save button.

(25) Make sure the chamber is under vacuum, ie EVAC LED

is not blinking and remains illuminated (BLUE)

7. Shutting Down The Instrument

(26) Close the TM4000 Graphical User Interface (GUI)

(27) Turn off the Power Switch on the right side of the

TM4000.

NOTE: Always leave the chamber under vacuum when

system not in use. This helps keep dust and moisture out of

chamber to ensure the microscope remains in good

condition.

Page 3

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